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WNT5A Gene

protein-coding   GIFtS: 71
GCID: GC03M055474

Wingless-Type MMTV Integration Site Family, Member 5A

  See WNT5A-related diseases
at
MalaCards
 

Aliases
for WNT5A gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section
Aliases
Wingless-Type MMTV Integration Site Family, Member 5A1 2
WNT-5A Protein1 2
hWNT5A2
Protein Wnt-5a2

External Ids:    HGNC: 127841   Entrez Gene: 74742   Ensembl: ENSG000001142517   OMIM: 1649755   UniProtKB: P412213   

Export aliases for WNT5A gene to outside databases

Previous GC identifers: GC03M054370 GC03M054625 GC03M055356 GC03M055458 GC03M055457


Summaries
for WNT5A gene

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for WNT5A Gene:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These
proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell
fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both
the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor
frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating
developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this
gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript
variants. (provided by RefSeq, Jan 2012)

GeneCards Summary for WNT5A Gene:
WNT5A (wingless-type MMTV integration site family, member 5A) is a protein-coding gene. Diseases associated with WNT5A include robinow syndrome, autosomal dominant, and laband syndrome. GO annotations related to this gene include cytokine activity and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is WNT5B.

UniProtKB/Swiss-Prot: WNT5A_HUMAN, P41221
Function: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit
canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin
signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation
through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene
expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation.
Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells
and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for
extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits
type II collagen expression in chondrocytes

Gene Wiki entry for WNT5A Gene


Genomic Views
for WNT5A gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NT_022517.19  NC_018914.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the WNT5A gene promoter:
         GATA-3   AP-1   ATF-2   POU3F2 (N-Oct-5b)   POU3F2 (N-Oct-5a)   POU3F2   Egr-4   COMP1   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 7): WNT5A promoter sequence
   Search Chromatin IP Primers for WNT5A

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WNT5A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p21-p14   Ensembl cytogenetic band:  3p14.3   HGNC cytogenetic band: 3p21-p14

WNT5A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WNT5A gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M055474:  view genomic region     (about GC identifiers)

Start:
 55,499,743 bp from pter      End:
 55,523,973 bp from pter
Size:
 24,231 bases      Orientation:
 minus strand

Proteins
for WNT5A gene

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, antibodies-online, and/or GeneTex,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section
UniProtKB/Swiss-Prot: WNT5A_HUMAN, P41221 (See protein sequence)
Recommended Name: Protein Wnt-5a precursor  
Size: 380 amino acids; 42339 Da
Subunit: Homooligomer; disulfide-linked, leading to inactivation. Interacts with PORCN. Interacts with WLS (By
similarity)
Secondary accessions: A8K4A4 Q6P278
Alternative splicing: 2 isoforms:  P41221-1   P41221-2   

Explore the universe of human proteins at neXtProt for WNT5A: NX_P41221

Explore proteomics data for WNT5A at MOPED

Post-translational modifications: 

  • Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor
    binding (By similarity)1
  • Glycosylation is necessary for secretion but not for activity (By similarity)1
  • Palmitoylation at Ser-244 is required for efficient binding to frizzled receptors. It is also required for
    subsequent palmitoylation at Cys-104. Palmitoylation is necessary for proper trafficking to cell surface (By
    similarity)1
  • Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers,
    leading to inactivation of WNT5A (By similarity)1
  • Glycosylation2 at Asn114, Asn120, Asn312, Asn326

    • See WNT5A Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001243034.1  NP_003383.2  

    ENSEMBL proteins: 
     ENSP00000417310   ENSP00000420104   ENSP00000418184   ENSP00000264634  
    Reactome Protein details: P41221

    WNT5A Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant WNT5A Protein
    R&D Systems Recombinant & Natural Proteins for WNT5A (Wnt-5a)
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    OriGene Protein Over-expression Lysate for WNT5A
    OriGene MassSpec for WNT5A
    OriGene Custom Protein Services for WNT5A
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    Novus Biologicals WNT5A Proteins
    Novus Biologicals WNT5A Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for WNT5A

         		 Search eBioscience for Proteins for WNT5A 

         		 Search GeneTex for Proteins for WNT5A 

         		antibodies-online proteins for WNT5A (13 products) 

         		antibodies-online peptides for WNT5A

    WNT5A Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of WNT5A
    R&D Systems Antibodies for WNT5A (Wnt-5a)
    Cell Signaling Technology (CST) Antibodies for WNT5A 
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    Novus Biologicals WNT5A Antibodies
    Search for Antibodies for WNT5A at Abcam
    Cloud-Clone Corp. Antibodies for WNT5A
    ThermoFisher Antibody for WNT5A
    antibodies-online antibodies for WNT5A (67 products) 

    WNT5A Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for WNT5A
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for WNT5A
    Cloud-Clone Corp. CLIAs for WNT5A
    Search eBioscience for ELISAs for WNT5A 
    antibodies-online kits for WNT5A (13 products) 

    Domains /
    Families
    for WNT5A gene

    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GenesLikeMe)
    About This Section

    		 HGNC Gene Families:
    ENDOLIG: Endogenous ligands
    WNT: Wingless-type MMTV integration sites

    3 InterPro protein domains:
     IPR026538 Wnt5a
     IPR005817 Wnt
     IPR018161 Wnt_CS

    Graphical View of Domain Structure for InterPro Entry P41221

    ProtoNet protein and cluster: P41221

    1 Blocks protein domain: IPB005816 Secreted growth factor Wnt protein

    UniProtKB/Swiss-Prot: WNT5A_HUMAN, P41221
    Similarity: Belongs to the Wnt family


    Find genes that share domains with WNT5A           About GenesLikeMe


    Function
    for WNT5A gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
    About This Section
    Molecular Function:

         UniProtKB/Swiss-Prot Summary: WNT5A_HUMAN, P41221
    Function: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit
    canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin
    signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation
    through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene
    expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation.
    Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells
    and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for
    extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits
    type II collagen expression in chondrocytes

         Genatlas biochemistry entry for WNT5A:
    wingless-type MMTV integration site 5A,Drosophila wingless (wg) segment polarity gene homolog,expressed in
    developing face,ventral area of midbrain,limbs an in adult brain,lung,modulating cell fate and cell behavior
    during vertebrate development

         Summary: 
    During embryonic development, WNT5A as signaling molecule is secreted from the following cells:
                
    Neural Tube: Floor Plate Cells (Spinal Floor Plate)

    It affects the following cells:
    Neural Tube: Somatic Motor Neurons (Motor Neural Progenitor Domain)

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700sequence-specific DNA binding transcription factor activity IMP19847889
    GO:0005102receptor binding ----
    GO:0005109frizzled binding IPI18174455
    GO:0005110frizzled-2 binding IEA--
    GO:0005115receptor tyrosine kinase-like orphan receptor binding IPI18287027
         
    Find genes that share ontologies with WNT5A           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for WNT5A:
     Increased G1 DNA content  Synthetic lethal with imatinib 

         15 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Wnt5a):
     cellular  craniofacial  digestive/alimentary  embryogenesis  growth/size/body 
     hearing/vestibular/ear  homeostasis/metabolism  limbs/digits/tail  liver/biliary system  mortality/aging 
     nervous system  renal/urinary system  reproductive system  respiratory system  skeleton 

    Find genes that share phenotypes with WNT5A           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Wnt5atm1Amc for WNT5A

       genOway: Develop your customized and physiologically relevant rodent model for WNT5A

    miRNA
    Products:            		miRTarBase miRNAs that target WNT5A:
    hsa-mir-186-5p (MIRT021154), hsa-mir-16-5p (MIRT031969), hsa-mir-155-5p (MIRT020965), hsa-mir-30a-5p (MIRT005162), hsa-mir-92a-3p (MIRT049710), hsa-mir-21-5p (MIRT030983)

    Block miRNA regulation of human, mouse, rat WNT5A using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate WNT5A (see all 85):
    hsa-miR-576-3p hsa-miR-323-3p hsa-miR-579 hsa-miR-520f hsa-miR-300 hsa-miR-218-1* hsa-miR-200a hsa-miR-3074-3p
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat WNT5A

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for WNT5A

    Clone
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    OriGene ORF clones in mouse, rat for WNT5A
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    GenScript: all cDNA clones in your preferred vector: WNT5A (NM_003392)
    Sino Biological Human cDNA Clone for WNT5A
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WNT5A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WNT5A
    Addgene plasmids for WNT5A 

    Cell Line
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    ESI BIO PureStem Progenitor for WNT5A: 
    PureStem Z11, Meso Progenitor
    In Situ Assay
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         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNT5A

    Flow Cytometry
    Products:       
         		eBioscience FlowRNA Probe Sets ( VA1-12202) for WNT5A 


    Localization
    for WNT5A gene

    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
    About This Section
    Subcellular locations from UniProtKB/Swiss-Prot
    WNT5A_HUMAN, P41221: Secreted, extracellular space, extracellular matrix
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    extracellular5
    golgi apparatus3
    plasma membrane3
    cytoskeleton2
    nucleus2
    cytosol1
    lysosome1
    mitochondrion1

    Gene Ontology (GO): Selected cellular component terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region TAS--
    GO:0005578proteinaceous extracellular matrix IEA--
    GO:0005615extracellular space IDA18703641
    GO:0005788endoplasmic reticulum lumen TAS--
    GO:0005796Golgi lumen TAS--

    Find genes that share ontologies with WNT5A           About GenesLikeMe


    Pathways & Interactions
    for WNT5A gene

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GenesLikeMe, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
    About This Section





    SuperPaths for WNT5A About   (see all 26)  
    See pathways by source

    		      Powered
    by    		     
    SuperPathContained pathways About
    1Wnt signaling pathway (KEGG)
    		Wnt signaling pathway0.40
    		Wnt Signaling Pathway NetPath0.37
    		Wnt Signaling Pathways0.40
    2Wnt Pathway
    		Wnt Pathway0.56
    		WNT ligand biogenesis and trafficking0.56
    3CDK-mediated phosphorylation and removal of Cdc6
    		Asymmetric localization of PCP proteins0.75
    		WNT5A-dependent internalization of FZD40.00
    		PCP/CE pathway0.70
    		WNT5A-dependent internalization of FZD2, FZD5 and ROR20.00
    4beta-catenin independent WNT signaling
    		beta-catenin independent WNT signaling0.79
    		Ca2+ pathway0.43
    		Signaling by Wnt0.79
    5Wnt Signaling Pathway (WikiPathways)
    		Wnt Signaling Pathway0.55
    		DNA damage response (only ATM dependent)0.32
    		Wnt Signaling Pathway and Pluripotency0.55


    Find genes that share SuperPaths with WNT5A           About GenesLikeMe

    Pathways by source                                   See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for WNT5A
        Wnt Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for WNT5A (see all 8)
        GSK3 Signaling
    Molecular Mechanisms of Cancer
    Presenilin-Mediated Signaling
    Human Embryonic Stem Cell Pluripotency
    Colorectal Cancer Metastasis

    1 Cell Signaling Technology (CST) Pathway for WNT5A
        Wnt / Hedgehog / Notch

    1 Tocris Bioscience Pathway for WNT5A
        Wnt Pathway

    2 GeneGo (Thomson Reuters) Pathways for WNT5A
        Transcription Androgen Receptor nuclear signaling
    Translation Non-genomic (rapid) action of Androgen Receptor

    Selected BioSystems Pathways for WNT5A (see all 8)
        MicroRNAs in cardiomyocyte hypertrophy
    Wnt Signaling Pathway
    Wnt Signaling Pathway and Pluripotency
    DNA damage response (only ATM dependent)
    Wnt Signaling Pathway NetPath


    Selected Reactome Pathways for WNT5A (see all 8)
        WNT5A-dependent internalization of FZD2, FZD5 and ROR2
    negative regulation of TCF-dependent signaling by WNT ligand antagonists
    WNT5A-dependent internalization of FZD4
    TCF dependent signaling in response to WNT
    PCP/CE pathway


    Selected Kegg Pathways  (Kegg details for WNT5A) (see all 8):
        Wnt signaling pathway
    Hedgehog signaling pathway
    Hippo signaling pathway
    Melanogenesis
    HTLV-I infection

        Pathway & Disease-focused RT2 Profiler PCR Arrays including WNT5A (see all 10): 
              Wound Healing in human mouse rat
              WNT Signaling Pathway in human mouse rat
              Adipogenesis in human mouse rat
              Estrogen Receptor Signaling in human mouse rat
              Notch Signaling Targets in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for WNT5A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for WNT5A (P412213 ENSP000002646344) via UniProtKB, MINT, STRING, and/or I2D (see all 119)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    FZD1Q9UP383, ENSP000002879344I2D: score=3 STRING: ENSP00000287934
    FZD5Q134673, ENSP000003546074I2D: score=1 STRING: ENSP00000354607
    KLQ9UEF73, ENSP000003694424I2D: score=1 STRING: ENSP00000369442
    RYKP349253, ENSP000002960844I2D: score=1 STRING: ENSP00000296084
    ROR2Q019743, ENSP000003648604I2D: score=2 STRING: ENSP00000364860
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 130):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000187activation of MAPK activity IDA--
    GO:0001667ameboidal cell migration IEA--
    GO:0001736establishment of planar polarity IEA--
    GO:0001756somitogenesis IEA--
    GO:0001837epithelial to mesenchymal transition IEP12841867

    Find genes that share ontologies with WNT5A           About GenesLikeMe



    Drugs & Compounds
    for WNT5A gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, Sets of similar genes according to GenesLikeMe)
    About This Section
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for WNT5A

    5 Novoseek inferred chemical compound relationships for WNT5A gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glycogen 33.3 6 10873564 (1), 18407462 (1), 17986005 (1), 12915680 (1) (see all 5)
    tyrosine 23.2 12 18615587 (3), 17986005 (2), 17463182 (1), 19561643 (1) (see all 8)
    retinoic acid 0.428 9 15592517 (3), 15925444 (1)
    calcium 0 8 17463182 (2), 18929644 (2), 14766423 (1), 19420254 (1) (see all 6)
    paraffin 0 1 14514922 (1)



    Find genes that share compounds with WNT5A           About GenesLikeMe



    Transcripts
    for WNT5A gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section
    REFSEQ mRNAs for WNT5A gene (3 alternative transcripts): 
    NM_001256105.1  NM_003392.4  XM_006713324.1  

    Unigene Cluster for WNT5A:

    Wingless-type MMTV integration site family, member 5A
    Hs.643085  [show with all ESTs]
    Unigene Representative Sequence: NM_003392
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000474267(uc003dhm.3 uc010hmw.3 uc003dhn.3 uc010hmx.3)
    ENST00000493406 ENST00000497027 ENST00000482079 ENST00000497817 ENST00000264634

    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate WNT5A (see all 85):
    hsa-miR-576-3p hsa-miR-323-3p hsa-miR-579 hsa-miR-520f hsa-miR-300 hsa-miR-218-1* hsa-miR-200a hsa-miR-3074-3p
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    Clone
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    Addgene plasmids for WNT5A 
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for WNT5A
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WNT5A
      QuantiTect SYBR Green Assays in human, mouse, rat WNT5A
      QuantiFast Probe-based Assays in human, mouse, rat WNT5A
    Flow Cytometry
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    Additional mRNA sequence: 

    AK021503.1 AK090582.1 AK290375.1 AK290869.1 BC064694.1 BC074783.2 EF028086.1 

    6 DOTS entries:

    DT.95294716  DT.440946  DT.120885032  DT.100744120  DT.102824816  DT.70101564 

    Selected AceView cDNA sequences (see all 129):

    BE671209 AW238894 AL700501 BM746350 AU118341 N51822 AI339491 BF589063 
    AA932802 AW055279 BC074783 BX105276 BX503521 AI750799 AW069504 AL079945 
    CA429390 AI819089 AA903835 BE220278 BE045609 AI683487 AI634753 CB992663 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for WNT5A    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7
    SP1:              -     -     -           -     -                           
    SP2:              -     -     -                                             
    SP3:                                                                        
    SP4:                                                                        
    SP5:                    -     -                                             


    ECgene alternative splicing isoforms for WNT5A

    Expression
    for WNT5A gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GenesLikeMe, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    WNT5A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCATTCAGAT
    WNT5A Expression
    About this image


    WNT5A expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 16) fully expand
     
     Brain (Nervous System)    fully expand to see all 6 entries
             Adult Endothelial Cells Blood Brain Barrier
             Midbrain tegmentum
     
     Dermis (Integumentary System)    fully expand to see all 6 entries
             Dermal Mesenchyme Cells Dorsal Subectodermal Mesenchyme
     
     Neurons
             nGnG Amacrine Cells Inner Nuclear Layer
     
     Eye (Sensory Organs)    fully expand to see all 4 entries
             nGnG Amacrine Cells Inner Nuclear Layer
             Periocular Mesenchyme
     
     Fibroblasts
             Dermal Fibroblasts Dorsal Dermis
             Detroit 551
    WNT5A Protein expression data from MOPED1, PaxDb2 and MaxQB3:     About this image

    WNT5A Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.643085

    UniProtKB/Swiss-Prot: WNT5A_HUMAN, P41221
    Tissue specificity: Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue
    and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not
    in stromal cells (at protein level)

        Pathway & Disease-focused RT2 Profiler PCR Arrays including WNT5A (see all 10): 
              Wound Healing in human mouse rat
              WNT Signaling Pathway in human mouse rat
              Adipogenesis in human mouse rat
              Estrogen Receptor Signaling in human mouse rat
              Notch Signaling Targets in human mouse rat

    Primer
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    Orthologs
    for WNT5A gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of animals.

    Orthologs for WNT5A gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Wnt5a1 , 5 wingless-related MMTV integration site 5A1, 5 90.09(n)1
    98.68(a)1    		   14 (16.80 cM)5
    224181  NM_009524.31  NP_033550.21 
     285047505 
    chicken
    (Gallus gallus)    		 Aves WNT5A1 wingless-type MMTV integration site family, member more 84.74(n)
    90(a)    		   395703  NM_204887.1  NP_990218.1 
    lizard
    (Anolis carolinensis)    		 Reptilia WNT5A6
    		 wingless-type MMTV integration site family, member...
    		 89(a)
    		 1 ↔ 1
    		 2(161755890-161775075)
    African clawed frog
    (Xenopus laevis)    		 Amphibia wnt5a-B2 wingless-type MMTV integration site family, member more 78.67(n)    L19716.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii wnt5a2 wingless-type MMTV integration site family, member more 81.32(n)   30105  NM_130937.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea cwn-23 WNT-2 precursor 43(a)
    (best of 3)    		   IV(10068050-10070141)   --


    ENSEMBL Gene Tree for WNT5A (if available)
    TreeFam Gene Tree for WNT5A (if available)

    Paralogs
    for WNT5A gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68,Sets of similar genes according to GenesLikeMe)
    About This Section

    Paralogs for WNT5A gene
    WNT5B2  WNT162  WNT62  WNT112  WNT22  WNT2B2  WNT3A2  WNT12  
    WNT42  WNT7A2  WNT7B2  WNT32  
    17 SIMAP similar genes for WNT5A using alignment to 4 protein entries:     WNT5A_HUMAN (see all proteins):
    WNT5B    WNT7B    WNT2B    WNT4    WNT2    WNT7A
    WNT3    WNT3A    WNT8A    WNT11    WNT8B    WNT16
    WNT1    WNT6    WNT9A    WNT9B    WNT10B

    Find genes that share paralogs with WNT5A           About GenesLikeMe



    Genomic Variants
    for WNT5A gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section


    Selected SNPs for WNT5A (see all 466)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 3 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    VAR_0666234
    		Robinow syndrome autosomal dominant (DRS)4--see VAR_0666232 C S mis40--------
    VAR_0666294
    		Robinow syndrome autosomal dominant (DRS)4--see VAR_0666292 C R mis40--------
    rs5903861,2
    		C,F,O,H--55432052(-) AAAACG/AATGTT 2 -- ds500121Minor allele frequency- A:0.13MN NS EA NA WA 2302
    rs795865241,2
    		F--55432053(+) ACATCG/ATTTTA 2 -- ds50012Minor allele frequency- A:0.04WA NA 238
    rs5895571,2
    		C,F--55432224(-) CATTTT/GTCTTT 2 -- ds500111Minor allele frequency- G:0.12EA NA MN WA 663
    rs764434201,2
    		F--55432272(+) ACCATA/GTCATC 2 -- ds50011Minor allele frequency- G:0.01WA 118
    rs1892330101,2
    		--55432296(+) TGTACA/GTGTGT 2 -- ds50010--------
    rs124972541,2
    		C,F,H--55432309(+) TTCTGA/GATACC 2 -- ds500130Minor allele frequency- N:0.00EA NA NS CSA WA 2962
    rs10478981,2
    		C,F,A,H--55432426(-) TTCATG/ATCTTG 2 -- ut3115Minor allele frequency- A:0.47MN NS EA NA CSA WA 970
    rs1816804181,2
    		--55432586(+) ACATTA/TAAAAA 2 -- ut310--------

    HapMap Linkage Disequilibrium report for WNT5A (55499743 - 55523973 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for WNT5A:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv517851CNV Gain19592680
    dgv1458e1CNV Complex17122850

    Human Gene Mutation Database (HGMD): WNT5A
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing WNT5A
    DNA2.0 Custom Variant and Variant Library Synthesis for WNT5A

    Disorders / Diseases
    for WNT5A gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB, Sets of similar genes according to GenesLikeMe)
    About This Section

    		 OMIM gene information: 164975   
    OMIM disorders: 180700  
    UniProtKB/Swiss-Prot: WNT5A_HUMAN, P41221
  • Robinow syndrome autosomal dominant (DRS) [MIM:180700]: A disease characterized by short-limb dwarfism,
    costovertebral segmentation defects and abnormalities of the head, face and external genitalia. The clinical
    signs are generally milder in dominant cases. Note=The disease is caused by mutations affecting the gene
    represented in this entry

    • 9 diseases for WNT5A:    
    About MalaCards
    robinow syndrome, autosomal dominant    laband syndrome    wnt5a-related robinow syndrome, autosomal dominant    robinow syndrome
    zimmermann-laband syndrome    epstein-barr virus-associated gastric carcinoma    mayer-rokitansky-kuster-hauser syndrome    breast cancer
    prostate cancer

    1 inferred disease relationship from the University of Copenhagen DISEASES database for WNT5A:
    Melanoma

    Find genes that share disorders with WNT5A           About GenesLikeMe

    Selected Novoseek inferred disease relationships for WNT5A gene (see all 14)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    breast cancer 38.3 51 18927296 (4), 15701836 (3), 19237581 (3), 16880514 (3) (see all 18)
    invasive cancer 28.7 2 19901340 (1), 9815976 (1)
    metastasis 27 13 18927296 (2), 19901340 (2), 16880514 (2), 19244247 (1) (see all 8)
    tumors 25.8 38 11809689 (4), 16314637 (4), 15735754 (3), 15701836 (2) (see all 18)
    cancer 23.2 19 19603030 (4), 9367869 (2), 9716023 (2), 18294932 (1) (see all 11)
    melanoma 22.6 17 19901340 (4), 19470740 (3), 14514922 (2), 17992121 (1) (see all 9)
    mammary tumor 21.5 2 12471617 (1), 19519951 (1)
    familial adenomatous polyposis 10.4 1 17463182 (1)
    primary tumor 7.6 1 15701836 (1)
    carcinoma squamous cell 3.42 6 16314637 (3), 19467340 (1), 19956879 (1), 12841867 (1)

    Genetic Association Database (GAD): WNT5A
    Human Genome Epidemiology (HuGE) Navigator: WNT5A (10 documents)

    Export disorders for WNT5A gene to outside databases

    Publications
    for WNT5A gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section


    PubMed articles for WNT5A gene, integrated from 10 sources (see all 284):
    (articles sorted by number of sources associating them with WNT5A)

    1. Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. (PubMed id 8288227)1, 2, 3, 9 Clark C.C.... Iozzo R.V. (Genomics 1993)
    2. Wnt-5a has tumor suppressor activity in thyroid carcinoma. (PubMed id 15735754)1, 2, 9 Kremenevskaja N.... Brabant G. (Oncogene 2005)
    3. WNT5A mutations in patients with autosomal dominant Robinow syndrome. (PubMed id 19918918)1, 2 Person A.D.... Lohr J.L. (Dev. Dyn. 2010)
    4. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    5. Analysis of eight genes modulating interferon gamma and human genetic susceptibility to tuberculosis: a case-control association study. (PubMed id 20525402)1, 4 MAPller M....Hoal E.G. (BMC Infect. Dis. 2010)
    6. Studies with Wnt genes and nonsyndromic cleft lip and palate. (PubMed id 20890934)1, 4 Menezes R....Vieira A.R. (Birth Defects Res. Part A Clin. Mol. Teratol. 2010)
    7. Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. (PubMed id 20634891)1, 4 Jugessur A....Murray J.C. (PLoS ONE 2010)
    8. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility &quot;hot-spot&quot;. (PubMed id 20628624)1, 4 Johnatty S.E.... . (PLoS Genet. 2010)
    9. High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men. (PubMed id 19453261)1, 4 Yerges L.M....Zmuda J.M. (J. Bone Miner. Res. 2009)
    10. Variation in WNT genes is associated with non-syndromic cleft lip with or without cleft palate. (PubMed id 18413325)1, 4 Chiquet B.T....Hecht J.T. (Hum. Mol. Genet. 2008)

    External Searches for WNT5A gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing WNT5A gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 7474 HGNC: 12784 AceView: WNT5A Ensembl:ENSG00000114251 euGenes: HUgn7474
    ECgene: WNT5A Kegg: 7474 H-InvDB: WNT5A

    Other Databases showing WNT5A gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing WNT5A gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for WNT5A Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for WNT5A Genetics and Cytogenetics in Oncology and Haematology

    Intellectual Property
    for WNT5A gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for WNT5A gene:
    Search GeneIP for patents involving WNT5A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
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    About This Section


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