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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

WNT4 Gene

protein-coding GIFtS: 65
GCID: GC01M022443

wingless-type MMTV integration site family, member 4

Explore 36 diseases affiliated with
WNT4 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Wingless-Type MMTV Integration Site Family, Member 4¹ ²
WNT-4¹ ²
SERKAL² ⁵
Protein Wnt-4²

External Ids:	HGNC: 12783¹	Entrez Gene: 54361²	Ensembl: ENSG00000162552⁷	OMIM: 603490⁵	UniProtKB: P56705³

Export aliases for WNT4 gene to outside databases

Previous GC identifers: GC01M022016 GC01M021481 GC01M021592 GC01M021915 GC01M022190 GC01M022318 GC01M020688

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for WNT4:

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins

have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and

patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule

shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4

protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a

concerted role in both the control of female development and the prevention of testes formation. This gene and another

two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this

gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: WNT4_HUMAN, P56705

Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein.

May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over

only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast

tissue

Gene Wiki entry for WNT4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000001.10 NC_018912.1 NT_004610.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the WNT4 gene promoter:
AhR Spz1 p53 FOXO3 MyoD FOXO3b HEN1 FOXO3a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: WNT4 promoter sequence

Search SABiosciences Chromatin IP Primers for WNT4

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WNT4

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.23-p35.1 Ensembl cytogenetic band: 1p36.12 HGNC cytogenetic band: 1p36.23-p35.1

WNT4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WNT4 gene location

GeneLoc information about chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01M022443: view genomic region (about GC identifiers)

Start:	22,443,798 bp from pter	End:	22,470,462 bp from pter
Size:	26,665 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: WNT4_HUMAN, P56705 (See protein sequence)

Recommended Name: Protein Wnt-4 precursor

Size: 351 amino acids; 39052 Da

Subunit: Interacts with PORCN (By similarity)

Subcellular location: Secreted, extracellular space, extracellular matrix

Secondary accessions: Q5TZQ0 Q96T81 Q9BXF5 Q9H1J8 Q9UJM2

Explore the universe of human proteins at neXtProt for WNT4: NX_P56705

Post-translational modifications:

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent

palmitoylation at Cys-78. Palmitoylation is necessary for proper trafficking to cell surface (By similarity)¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P56705

WNT4 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_110388.2

ENSEMBL proteins:

ENSP00000290167 ENSP00000403334 ENSP00000388925 ENSP00000441033

Reactome Protein details: P56705
Human Recombinant Protein Products for WNT4:

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	Novus Biologicals WNT4 Protein Novus Biologicals WNT4 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for WNT4

Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005578	proteinaceous extracellular matrix	IEA	--
GO:0005615	extracellular space	IDA	16959810
GO:0005737	cytoplasm	IDA	16959810
GO:0005794	Golgi apparatus	IBA	--
GO:0005886	plasma membrane	IBA	--

WNT4 for ontologies About GeneDecksing

WNT4 Antibody Products:

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	ThermoFisher Antibodies for WNT4

Assay Products for WNT4:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for WNT4
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for WNT4

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

WNT4 for domains About GeneDecksing

3 InterPro domains/families:

IPR009142 Wnt4

IPR005817 Wnt

IPR018161 Wnt_CS

Graphical View of Domain Structure for InterPro Entry P56705

ProtoNet protein and cluster: P56705

1 Blocks protein family: IPB005816 Secreted growth factor Wnt protein

UniProtKB/Swiss-Prot: WNT4_HUMAN, P56705

Similarity: Belongs to the Wnt family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: WNT4_HUMAN, P56705

Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein.

May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over

only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast

tissue

Summary:

During embryonic development, WNT4 as signaling molecule is secreted from the following cells: Interzone Cells in Stylopod Synovial Joint, Interzone Cells in Zeugopod Synovial Joint (see all 4).

It affects the following cells: Articular Cartilage Cells in Autopod Articular Cartilage, Articular Cartilage Cells in Zeugopod Articular Cartilage (see all 4).

Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001664	G-protein coupled receptor binding	IBA	--
GO:0003714	transcription corepressor activity	ISS	--
GO:0005102	receptor binding	--	--
GO:0005109	frizzled binding	IEA	--
GO:0005201	extracellular matrix structural constituent	NAS	--

WNT4 for ontologies About GeneDecksing

Phenotypes:
14 MGI mutant phenotypes (inferred from 10 alleles) (MGI details for Wnt4):

cellular	embryogenesis	endocrine/exocrine gland	growth/size	hematopoietic system
immune system	integument	limbs/digits/tail	mortality/aging	nervous system
no phenotypic analysis	normal	renal/urinary system	reproductive system

WNT4 for phenotypes About GeneDecksing

Animal Models:
Mouse knock-outs for WNT4: Wnt4^tm1.2Boer Wnt4^tm1Amc Wnt4^tm1.2Bhr

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for WNT4

miRNA Products:		OriGene 3'-UTR Clone: WNT4 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat WNT4
		8/47 QIAGEN miScript miRNA Assays for microRNAs that regulate WNT4 (see all 47):
		hsa-miR-194* hsa-miR-517a hsa-miR-15a hsa-miR-517c hsa-miR-503 hsa-miR-650 hsa-miR-29b-1* hsa-miR-424

		SwitchGear 3'UTR luciferase reporter plasmid: WNT4 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WNT4 (see all 7) OriGene shRNA RFP: WNT4 OriGene siRNA: WNT4
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		Sirion Biotech Custom design and validation of potent shRNA sequences against WNT4

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for WNT4
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Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WNT4 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WNT4 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: WNT4 (NM_030761)
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Cell Line Products:		GenScript Custom overexpressing Cell Line Services for WNT4
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		Sirion Biotech Customized stable knockdown cell line services for WNT4 Sirion Biotech Customized inducible knockdown cell line services for WNT4 Sirion Biotech Customized stable overexpressing cell line services for WNT4 Sirion Biotech Customized inducible overexpressing cell line services for WNT4

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNT4

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/14 super-pathways (see all 14) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Wnt Signaling Pathway

Wnt Signaling Pathway	1.00	Wnt Signaling Pathway	0.33
Wnt Signaling Pathway and Pluripotency	0.55	Wnt signaling pathway	0.30
DNA damage response (only ATM dependent)	0.35	Wnt Signaling Pathway NetPath	0.22

Basal cell carcinoma

	Basal cell carcinoma	1.00			Wnt Pathway	0.37
	Hedgehog signaling pathway	0.41			Melanogenesis	0.32

Translation_Non-genomic (rapid) action of Androgen Receptor

Translation_Non-genomic (rapid) action of Androgen Receptor

1.00

Translation Non-genomic (rapid) action of Androgen Receptor

0.99

Transcription_Androgen Receptor nuclear signaling

Transcription_Androgen Receptor nuclear signaling

1.00

Transcription Androgen Receptor nuclear signaling

0.99

Nanog in Mammalian ESC Pluripotency

Nanog in Mammalian ESC Pluripotency

1.00

GSK3 Signaling

0.61

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for WNT4

	Transcription Androgen Receptor nuclear signaling
	Translation Non-genomic (rapid) action of Androgen Receptor

1 R&D Systems Pathway for WNT4

Wnt Signaling Pathway

5/7 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for WNT4 (see all 7)

	GSK3 Signaling
	Presenilin-Mediated Signaling
	Colorectal Cancer Metastasis
	Human Embryonic Stem Cell Pluripotency
	Nanog in Mammalian ESC Pluripotency

1 Tocris Bioscience Pathway for WNT4

Wnt Pathway

2 GeneGo (Thomson Reuters) Pathways for WNT4

	Transcription Androgen Receptor nuclear signaling
	Translation Non-genomic (rapid) action of Androgen Receptor

4 BioSystems Pathways for WNT4

	DNA damage response (only ATM dependent)
	Wnt Signaling Pathway and Pluripotency
	Wnt Signaling Pathway
	Wnt Signaling Pathway NetPath

4 Reactome Pathways for WNT4

	Signal Transduction
	Signaling by GPCR
	GPCR ligand binding
	Class B/2 (Secretin family receptors)

5 Kegg Pathways (Kegg details for WNT4):

	Wnt signaling pathway
	Hedgehog signaling pathway
	Melanogenesis
	Pathways in cancer
	Basal cell carcinoma

WNT4 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for WNT4

STRING Interaction Network Preview (showing 5 interactants - click image to see 19)

5/45 Interacting proteins for WNT4 (P56705³ ENSP00000290167⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 45)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
FZD1	Q9UP38³, ENSP00000287934⁴	I2D: score=2 STRING: ENSP00000287934
FZD5	Q13467³, ENSP00000354607⁴	I2D: score=2 STRING: ENSP00000354607
FZD6	O60353³, ENSP00000351605⁴	I2D: score=2 STRING: ENSP00000351605
PTEN	P60484³, ENSP00000361021⁴	I2D: score=1 STRING: ENSP00000361021
PORCN	Q9H237³, ENSP00000322304⁴	I2D: score=5 STRING: ENSP00000322304

About this table

Gene Ontology (GO): 5/71 biological process terms (GO ID links to tree view) (see all 71): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001656	metanephros development	IBA	--
GO:0001658	branching involved in ureteric bud morphogenesis	IBA	--
GO:0001822	kidney development	IEP	15312687
GO:0001823	mesonephros development	--	--
GO:0001837	epithelial to mesenchymal transition	IEP	12841867

WNT4 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

WNT4 for compounds About GeneDecksing

EMD Millipore small molecules for WNT4:
Small Molecule - inhibitor

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for WNT4
2 Novoseek chemical compound relationships for WNT4 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
testosterone	13.4	2	15317892 (1), 12949260 (1)
progesterone	6.8	2	17711857 (1), 12949260 (1)

Search CenterWatch for drugs/clinical trials and news about WNT4

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for WNT4 gene:

NM_030761.4

Unigene Cluster for WNT4:

Wingless-type MMTV integration site family, member 4

Hs.25766 [show with all ESTs]

Unigene Representative Sequence: NM_030761

4 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000290167(uc001bfs.4) ENST00000415567 ENST00000441048 ENST00000542383(uc010odt.2)

miRNA Products:		OriGene 3'-UTR Clone: WNT4 Browse OriGene MicroRNA Expression Plasmids
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		8/47 QIAGEN miScript miRNA Assays for microRNAs that regulate WNT4 (see all 47):
		hsa-miR-194* hsa-miR-517a hsa-miR-15a hsa-miR-517c hsa-miR-503 hsa-miR-650 hsa-miR-29b-1* hsa-miR-424

		SwitchGear 3'UTR luciferase reporter plasmid: WNT4 3' UTR sequence

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		Sirion Biotech Custom design and validation of potent shRNA sequences against WNT4

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		GenScript: all cDNA clones in your preferred vector: WNT4 (NM_030761)
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		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WNT4
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WNT4

Additional cDNA sequence:

AB061675.1 AF316543.1 AK296058.1 AY009398.1 AY358947.1 BC007386.2 BC057781.1 BT020125.1

3 DOTS entries:

DT.70104953 DT.70105154 DT.97800841

24/50 AceView cDNA sequences (see all 50):

NM_030761 BQ268938 AI139742 BC057781 CK821661 BE218554 AI634496 BM128213

CK821662 AW137060 AF316543 BQ269212 BF448386 AY009398 BM894135 AY358947

AI971394 AI871742 BE549894 BU947973 BM673340 BM544112 AI267902 AI634480

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

WNT4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CAACAAGACG

About this image

WNT4 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

10/25 LifeMap In Vivo Development Anatomical Compartments/Cells (see all 25)

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Kidney

Pretubular Aggregates

Pretubular Aggregate Cells

Selective marker (cell-identifying gene)

Kidney

Ovary

Ovigerous Cord

Ovarian Somatic Cells

Ovary

Cartilage

Autopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Stylopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Zeugopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Kidney

Renal Vesicle

Distal Renal Vesicle Cells

Kidney

Renal Vesicle

Proximal Renal Vesicle Cells

Kidney

S-shaped Body Medial Segment

S-shaped Body Medial Cells

Kidney

Lateral Plate Mesoderm

Intraembryonic Coelom

Coelomic Epithelial Cells

Reproductive System

Limb

Autopod Synovial Joint

Interzone Cells

Cartilage

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 4 LifeMap Cells

Name

Category

mK4 (Cell line)

Kidney

mK3 (Cell line)

Kidney

Secondary follicles (Primary Cell)

Ovary, Reproductive System

Renal-like cells (Derivation of renal-...)

See WNT4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for WNT4

SOURCE GeneReport for Unigene cluster: Hs.25766

SABiosciences Expression via Pathway-Focused PCR Arrays including WNT4:

Notch Signaling Targets in human mouse rat

Estrogen Receptor Signaling in human mouse rat

Hedgehog Signaling Pathway in human mouse rat

WNT Signaling Pathway in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WNT4 Browse OriGene validated miRNA SYBR primer pairs
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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WNT4

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNT4

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for WNT4 gene from 6/20 species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	WNT4¹	wingless-type MMTV integration site family, member more	81.2(n) 85.47(a)		395561 NM_204783.1 NP_990114.1
lizard (Anolis carolinensis)	Reptilia	WNT4⁶	--	87(a)	1 ↔ 1	AAWZ02037479(11289-12989)
African clawed frog (Xenopus laevis)	Amphibia	Xwnt-4²	(Xwnt-4)	77.18(n)		U13183.1
zebrafish (Danio rerio)	Actinopterygii	wnt4a²	wnt4a	80.56(n)		30123 U25141.1
honey bee (Apis mellifera)	Insecta	--	--	40(a)	1 ↔ 1	Group1.29(123474-142553)
worm (Caenorhabditis elegans)	Secernentea	cwn-1^{1 , 3}	Expression: embryo, L1 larva, L2 larva, L3 larva, more³ Protein CWN-1¹	46(a) (best of 5)³ 50.64(n)¹ 46.88(a)¹		II(131165-133577)³ 173399¹ NM_061267.3¹ NP_493668.1¹

ENSEMBL Gene Tree for WNT4 (if available)
TreeFam Gene Tree for WNT4 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for WNT4 gene

WNT5B² WNT16² WNT5A² WNT6² WNT11² WNT2² WNT2B² WNT3A²

WNT1² WNT10B² WNT7A² WNT7B² WNT10A² WNT3²

18 SIMAP similar genes for WNT4 using alignment to 5 protein entries: WNT4_HUMAN (see all proteins):

WNT2 WNT2B WNT3A WNT3 WNT7A WNT5A

WNT7B WNT16 WNT5B WNT11 WNT10B WNT1

WNT9B WNT9A WNT6 WNT8B WNT8A WNT10A

WNT4 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 1 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs35008390^1,2	C	--	22443317(+)	`GATCTC/-CCCAT`	1	--	ds500¹		1		NA	2
rs58119053^1,2	C	--	22443319(+)	`TCCCA-/CTCAGC`	1	--	ds500¹		0	--	--	--	--
rs12406495^1,2	H	--	22443465(+)	`AGAAAA/CTAAGT`	1	--	ds500¹		0	--	--	--	--
rs72879291^1,2	C,F	--	22443482(+)	`CCCTCA/TTCCCC`	1	--	ds500¹		4		WA CSA NA	242
rs144742430^1,2		--	22443587(+)	`AGCTGA/GTCATG`	1	--	ds500¹		0	--	--	--	--
rs2235530^1,2	C,F,H	--	22443760(-)	`GAAGAG/AACTTG`	1	--	ds500¹		17		EA NS NA CSA	3416
rs114373349^1,2	F	--	22443763(+)	`GTCTCT/GTCTCC`	1	--	ds500¹		1		WA	118
rs148106570^1,2		--	22443796(+)	`GTCACA/GATTGC`	1	--	ds500¹		0	--	--	--	--
rs190231992^1,2	C	--	22443827(+)	`TTATCA/GTGCAT`	1	--	ut3¹		0	--	--	--	--
rs141883380^1,2		--	22443862(+)	`ATGGCA/CAAGAG`	1	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for WNT4 (22443798 - 22470462 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for WNT4: --
Human Gene Mutation Database (HGMD): WNT4

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing WNT4

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

WNT4 for disorders           About GeneDecksing

OMIM gene information: 603490
OMIM disorders: 611812  158330
UniProtKB/Swiss-Prot: WNT4_HUMAN, P56705

Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called

Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by

utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the

genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has

an incidence of approximately 1 in 5'000 newborn girls

Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL)

[MIM:611812]; also known as SERKAL syndrome

Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330]

20/36 diseases for WNT4 (see all 36): About MalaCards

rokitansky-kuster-hauser syndrome mayer-rokitansky-kuster-hauser syndrome 46, xy disorders of sex development serkal syndrome

dosage-sensitive sex reversal disorders of sex development gonadal dysgenesis mullerian aplasia

sex reversal mullerian aplasia and hyperandrogenism premature ovarian failure virilization

polycystic ovary syndrome hyperandrogenism basal cell carcinoma chronic lymphocytic leukemia

breast-ovarian cancer eaf lymphocytic leukemia pituitary adenoma

3 Novoseek disease relationships for WNT4 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
virilization	60.8	4	15317892 (1), 15988165 (1), 15581876 (1), 11283799 (1)
tumors	6.47	1	8168088 (1)
breast cancer	0	1	16501043 (1)

GeneTests: WNT4

46,XY Disorder of Sex Development and 46,XY Complete Gonadal Dysgenesis

Human Genome Epidemiology (HuGE) Navigator: WNT4 (4 documents)

Export disorders for WNT4 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for WNT4 gene, integrated from 9 sources (see all 110):
(articles sorted by number of sources associating them with WNT4)

Utopia: connect your pdf to the dynamic
world of online information

Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. (PubMed id 8168088)^{1, 2, 3, 9} Huguet E.L.... Harris A.L. (1994)
WNT4 deficiency-a clinical phenotype distinct from the classic Mayer- Rokitansky-Kuster-Hauser syndrome: a case report. (PubMed id 16959810)^{1, 2, 9} Biason-Lauber A....Schoenle E.J. (2007)
A WNT4 mutation associated with Muellerian-duct regression and virilization in a 46,XX woman. (PubMed id 15317892)^{1, 2, 9} Biason-Lauber A.... Schoenle E.J. (2004)
Up-regulation of wnt-4 signaling and dosage-sensitive sex reversal in humans. (PubMed id 11283799)^{1, 2, 9} Jordan B.K.... Vilain E. (2001)
Identification and functional analysis of a new WNT4 gene mutation among 28 adolescent girls with primary amenorrhea and Muellerian duct abnormalities: a French collaborative study. (PubMed id 18182450)^{1, 2} Philibert P.... Sultan C. (2008)
SERKAL syndrome: an autosomal-recessive disorder caused by a loss-of- function mutation in WNT4. (PubMed id 18179883)^{1, 2} Mandel H....Sprecher E. (2008)
The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)^{1, 2} Gregory S.G.... Bentley D.R. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)^{1, 2} Clark H.F.... Gray A.M. (2003)
Down-regulation of Wnt-4 and up-regulation of Wnt-5a expression by epithelial-mesenchymal transition in human squamous carcinoma cells. (PubMed id 12841867)^{1, 9} Taki M....Nagayama M. (2003)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 54361	HGNC: 12783	AceView: WNT4	Ensembl:ENSG00000162552	euGenes: HUgn54361
ECgene: WNT4	Kegg: 54361	H-InvDB: WNT4

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for WNT4	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for WNT4	Genetics and Cytogenetics in Oncology and Haematology
GeneReviews	http://www.ncbi.nlm.nih.gov/sites/GeneTests/lab/gene/WNT4

Intellectual Property for WNT4 gene
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Patent Information for WNT4 gene:
Search GeneIP for patents involving WNT4

GeneCards and IP:
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wingless-type MMTV integration site family, member 4

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wingless-type MMTV integration site family, member 4

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