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WNK2 Gene

protein-coding   GIFtS: 62
GCID: GC09P095947

WNK Lysine Deficient Protein Kinase 2

(Previous name: serologically defined colon cancer antigen 43)
(Previous symbols: SDCCAG43, PRKWNK2)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
WNK Lysine Deficient Protein Kinase 21 2     NY-CO-432
Serologically Defined Colon Cancer Antigen 431 2 3     P/OKcl.132
PRKWNK21 2 3     Mitogen-Activated Protein Kinase Kinase Kinase2
SDCCAG431 2 3     Protein Kinase, Lysine Deficient 22
Antigen NY-CO-432 3     Serine/Threonine-Protein Kinase WNK22
Protein Kinase Lysine-Deficient 22 3     KIAA17603
Protein Kinase With No Lysine 22 3     PRK5
EC 2.7.11.13 8     EC 2.7.118

External Ids:    HGNC: 145421   Entrez Gene: 652682   Ensembl: ENSG000001652387   OMIM: 6062495   UniProtKB: Q9Y3S13   

Export aliases for WNK2 gene to outside databases

Previous GC identifers: GC09P093027 GC09P094987 GC09P065623


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for WNK2 Gene:
The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase
superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling
survival, and proliferation. Alternative splicing results in multiple transcript variants. (provided by RefSeq,
Sep 2013)

GeneCards Summary for WNK2 Gene:
WNK2 (WNK lysine deficient protein kinase 2) is a protein-coding gene. Diseases associated with WNK2 include pseudohypoaldosteronism type ii, and pseudohypoaldosteronism. GO annotations related to this gene include protein serine/threonine kinase activity. An important paralog of this gene is WNK1.

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1
Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell
signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride
cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B,
SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and
the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this
modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by
controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display
reciprocal activity

Gene Wiki entry for WNK2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000009.12  NT_008470.20  NC_018920.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the WNK2 gene promoter:
         C/EBPbeta   RP58   AP-1   ATF-2   Lmo2   MyoD   Egr-4   Evi-1   c-Jun   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidWNK2 promoter sequence
   Search Chromatin IP Primers for WNK2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WNK2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q22.3   Ensembl cytogenetic band:  9q22.31   HGNC cytogenetic band: 9q22.3

WNK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WNK2 gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P095947:  view genomic region     (about GC identifiers)

Start:
95,947,198 bp from pter      End:
96,082,854 bp from pter
Size:
135,657 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase WNK2  
Size: 2297 amino acids; 242676 Da
Cofactor: Magnesium (By similarity)
Subunit: Forms a complex with the phosphorylated form of STK39 (By similarity)
Caution: Cys-224 is present instead of the conserved Lys which is expected to be an active site residue. Lys-207
appears to fulfill the required catalytic function
Sequence caution: Sequence=AAH37965.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: Q5VWF1 Q5VWF2 Q8IY36 Q9C0A3 Q9H3P4
Alternative splicing: 4 isoforms:  Q9Y3S1-1   Q9Y3S1-2   Q9Y3S1-3   Q9Y3S1-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for WNK2: NX_Q9Y3S1

Explore proteomics data for WNK2 at MOPED

Post-translational modifications: 

  • Autophosphorylated1
  • Ubiquitination2 at Lys349
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for WNK2 (Q9Y3S1) (see all 13)
     EYPYSEC  IEQMKDV  YDESVDV  PPIIHRD 


    See WNK2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001269323.1  NP_006639.3  

    ENSEMBL proteins: 
     ENSP00000412465   ENSP00000297954   ENSP00000415038   ENSP00000414622   ENSP00000390441  
     ENSP00000413325   ENSP00000378860   ENSP00000378858   ENSP00000297876   ENSP00000411181  
     ENSP00000348347  
    Reactome Protein details: Q9Y3S1

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: WNK lysine deficient protein kinase 2
    Wnk family

    4 InterPro protein domains:
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom
     IPR024678 Kinase_OSR1/WNK_CCT

    Graphical View of Domain Structure for InterPro Entry Q9Y3S1

    ProtoNet protein and cluster: Q9Y3S1

    UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1
    Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily
    Similarity: Contains 1 protein kinase domain


    WNK2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: WNK2_HUMAN, Q9Y3S1
    Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell
    signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride
    cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B,
    SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and
    the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this
    modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by
    controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display
    reciprocal activity
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes
    increased activity (By similarity)

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity ISS--
    GO:0004713protein tyrosine kinase activity ----
    GO:0005524ATP binding ISS--
    GO:0016772transferase activity, transferring phosphorus-containing groups ----
         
    WNK2 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for WNK2:
     Increased cell death HMECs cel 

         1 MGI phenotypic allele for Wnk2 (no phenotypes)

    WNK2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Wnk2tm1Lex for WNK2

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    WNK2_HUMAN, Q9Y3S1: Cytoplasm. Cell membrane
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    nucleus3
    cytosol2
    endosome2
    lysosome1
    vacuole1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA17667937
    GO:0005886plasma membrane IEA--

    WNK2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for WNK2 About                                                                                                See pathways by source

    SuperPathContained pathways About
    1Ion channel transport
    Ion channel transport0.58
    Stimuli-sensing channels0.58
    2Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds
    Transmembrane transport of small molecules0.47

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for WNK2
        Stimuli-sensing channels



    WNK2 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for WNK2
    Interactions:

        GeneGlobe Interaction Network for WNK2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

    Selected Interacting proteins for WNK2 (Q9Y3S12, 3 ENSP000002979544) via UniProtKB, MINT, STRING, and/or I2D (see all 8)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABL1P005192, 3, ENSP000003614234MINT-8110875 I2D: score=3 STRING: ENSP00000361423
    FYNP062412, 3, ENSP000003576564MINT-8111619 I2D: score=3 STRING: ENSP00000357656
    ATXN1P542533, ENSP000002447694I2D: score=2 STRING: ENSP00000244769
    UBE2IP632793, ENSP000003248974I2D: score=1 STRING: ENSP00000324897
    YWHAZP631043, ENSP000003095034I2D: score=2 STRING: ENSP00000309503
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468protein phosphorylation IDA--
    GO:0008285negative regulation of cell proliferation IMP17667937
    GO:0035556intracellular signal transduction ISS--
    GO:0046777protein autophosphorylation IDA17667937
    GO:0070373negative regulation of ERK1 and ERK2 cascade IMP17667937

    WNK2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for WNK2

    3 HMDB Compounds for WNK2    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for WNK2 gene (2 alternative transcripts): 
    NM_001282394.1  NM_006648.3  

    Unigene Cluster for WNK2:

    WNK lysine deficient protein kinase 2
    Hs.654856  [show with all ESTs]
    Unigene Representative Sequence: AB051547
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19):
    ENST00000448039(uc010mrd.1) ENST00000297954(uc004ati.1) ENST00000432730(uc011lud.1)
    ENST00000411624(uc004atk.3) ENST00000464625 ENST00000478583 ENST00000448251(uc004atl.1)
    ENST00000462595 ENST00000453718 ENST00000474009 ENST00000479696 ENST00000467401
    ENST00000460335 ENST00000471076 ENST00000395477(uc004atj.3) ENST00000395475(uc010mrc.1)
    ENST00000349097 ENST00000427277
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    Additional mRNA sequence: 

    AB044546.1 AB051547.2 AJ242724.4 AK000694.1 AK055151.1 AK093910.1 AK124314.1 AK310390.1 
    BC037965.1 

    14 DOTS entries:

    DT.100022041  DT.100739040  DT.91801966  DT.442052  DT.95325142  DT.425812  DT.91651393  DT.99999119 
    DT.40218586  DT.100016290  DT.121164654  DT.40109714  DT.92419749  DT.91789567 

    Selected AceView cDNA sequences (see all 110):

    BU732203 CA946368 BQ925250 BC037965 BG231979 BQ637666 AA367042 AL134251 
    BE561168 AI217214 BQ222235 AI632414 BM671000 AB051547 AJ242724 AA970341 
    BM703415 AA368833 AW500011 AW419087 AW001397 AW337451 NM_006648 W73992 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for WNK2 (see all 17)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c ^ 13 ^ 14 ^ 15a · 15b ^ 16a · 16b ^ 17 ^ 18a · 18b ^
    SP1:                                                                                                                                            -               
    SP2:                    -                                               -                                                                                       
    SP3:                                                                                                                                            -               
    SP4:                                                                                                                                                            
    SP5:                                                                                                              -                             -               

    ExUns: 19 ^ 20 ^ 21a · 21b ^ 22a · 22b · 22c ^ 23 ^ 24 ^ 25 ^ 26a · 26b ^ 27 ^ 28 ^ 29 ^ 30 ^ 31a · 31b · 31c ^ 32a · 32b ^ 33 ^ 34 ^ 35
    SP1:              -                                                                                   -                 -           -     -         
    SP2:                                                                                                                                                
    SP3:              -                                                                                                                                 
    SP4:                                                                                                                                                
    SP5:                                                                                                                                                


    ECgene alternative splicing isoforms for WNK2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    WNK2 expression in normal human tissues (normalized intensities)      WNK2 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GTGGTCAGTG
    WNK2 Expression
    About this image


    WNK2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Ovary (Reproductive System)
             Oviduct
     
     Brain (Nervous System)
             Cerebellum
    WNK2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    WNK2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.654856

    UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1
    Tissue specificity: Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart,
    brain, skeletal muscle and colon

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for WNK2 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Wnk21 , 5 WNK lysine deficient protein kinase 21, 5 84.44(n)1
    86.2(a)1
      13 (25.07 cM)5
    756071  NM_029361.31  NP_083637.21 
     490363035 
    chicken
    (Gallus gallus)
    Aves WNK21 WNK lysine deficient protein kinase 2 66.27(n)
    67.77(a)
      415984  XM_414326.4  XP_414326.4 
    lizard
    (Anolis carolinensis)
    Reptilia WNK26
    WNK lysine deficient protein kinase 2
    58(a)
    1 ↔ 1
    GL343225.1(1588925-1672311)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia wnk21 WNK lysine deficient protein kinase 2 65.74(n)
    61.11(a)
      100488072  XM_002943063.2  XP_002943109.2 
    zebrafish
    (Danio rerio)
    Actinopterygii WNK26
    WNK lysine deficient protein kinase 2
    34(a)
    1 ↔ 1
    Zv9_NA994(132-19741) ENSDARG00000077051
    fruit fly
    (Drosophila melanogaster)
    Insecta Wnk6
    --
    21(a)
    1 → many
    3L(21536950-21548028)
    worm
    (Caenorhabditis elegans)
    Secernentea wnk-16
    Protein WNK-1, isoform l (wnk-1) mRNA, complete cd...
    25(a)
    1 → many
    IV(9200116-9212232) WBGene00006941
    rice
    (Oryza sativa)
    Liliopsida Os.22212 Oryza sativa mitogen activated protein kinase kinase more 77.64(n)    AK100426.1 


    ENSEMBL Gene Tree for WNK2 (if available)
    TreeFam Gene Tree for WNK2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for WNK2 gene
    WNK12  WNK42  NRBP12  NRBP22  WNK32  
    7 SIMAP similar genes for WNK2 using alignment to 11 protein entries:     WNK2_HUMAN (see all proteins):
    WNK3    STK11    WNK4    NEK6    CDK4    STK17A
    WNK1

    WNK2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for WNK2 (see all 3109)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2074703751,2
    Cuntested196055487(+) CCCCCA/G/TCCCCT 1 -- int10--------
    rs1386953821,2
    C,Funtested196061517(+) AGCCAG/ATGATG 2 /N /S mis12Minor allele frequency- A:0.00NA EU 5047
    rs557995421,2
    C--65661820(+) CCAGG-/TGGCCTT 1 -- int11Minor allele frequency- TG:0.00NA 2
    rs1471691411,2
    C--95945238(+) CAGGC-/TACAGG 1 -- us2k10--------
    rs1909375851,2
    --95945327(+) CCAGCA/GGGGCT 1 -- us2k10--------
    rs767865281,2
    C,F--95945359(+) GGTAAC/GAGTTG 1 -- us2k12Minor allele frequency- G:0.02NA 122
    rs1512541871,2
    C--95945759(+) AGAGAA/TCATGG 1 -- us2k10--------
    rs1828320471,2
    --95945976(+) GCCTCC/TCTGGG 1 -- us2k10--------
    rs1404240631,2
    --95946067(+) GGCTGC/GGCTCC 1 -- us2k10--------
    rs1148839711,2
    F--95946153(+) CGCGCC/TGGGCT 1 -- us2k11Minor allele frequency- T:0.13WA 118

    HapMap Linkage Disequilibrium report for WNK2 (95947198 - 96082854 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for WNK2 (see all 16):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2738782CNV Deletion23290073
    esv2668317CNV Deletion23128226
    esv2738781CNV Deletion23290073
    nsv509310CNV Insertion20534489
    esv1576972CNV Insertion17803354
    esv2506086CNV Insertion19546169
    esv1441694CNV Insertion17803354
    nsv893582CNV Loss21882294
    nsv415569CNV Loss16902084
    nsv893580CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing WNK2
    DNA2.0 Custom Variant and Variant Library Synthesis for WNK2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 606249    OMIM disorders: --

    8 diseases for WNK2:    About MalaCards
    pseudohypoaldosteronism type ii    pseudohypoaldosteronism    kidney disease    hypertension
    ataxia    pancreatic cancer    pancreatitis    neuronitis

    1 disease from the University of Copenhagen DISEASES database for WNK2:
    Pseudohypoaldosteronism

    WNK2 for disorders           About GeneDecksing

    Genetic Association Database (GAD): WNK2
    Human Genome Epidemiology (HuGE) Navigator: WNK2 (4 documents)

    Export disorders for WNK2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for WNK2 gene, integrated from 10 sources (see all 34):
    (articles sorted by number of sources associating them with WNK2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. WNK kinases, a novel protein kinase subfamily in multi-cellular organisms. (PubMed id 11571656)1, 2, 3 Verissimo F. and Jordan P. (Oncogene 2001)
    2. WNK2 modulates MEK1 activity through the Rho GTPase pathway. (PubMed id 18593598)1, 2, 9 Moniz S.... Jordan P. (Cell. Signal. 2008)
    3. WNK2 is a novel regulator of essential neuronal cation-chloride cotransporters. (PubMed id 21733846)1, 2 Rinehart J.... Lifton R.P. (J. Biol. Chem. 2011)
    4. Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals. (PubMed id 19851296)1, 4 Oguri M....Yamada Y. (Am. J. Hypertens. 2010)
    5. Association of genetic variants with hemorrhagic stroke in Japanese individuals. (PubMed id 20198315)1, 4 Yoshida T....Yamada Y. (Int. J. Mol. Med. 2010)
    6. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    7. Association of gene polymorphisms with chronic kidney disease in Japanese individuals. (PubMed id 19724895)1, 4 Yoshida T....Yamada Y. (Int. J. Mol. Med. 2009)
    8. Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2. (PubMed id 17667937)1, 2 Moniz S.... Jordan P. (Oncogene 2007)
    9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    10. Molecular basis of T cell-mediated recognition of pancreatic cancer cells. (PubMed id 11280764)1, 2 Ito M....Itoh K. (Cancer Res. 2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 65268 HGNC: 14542 AceView: PRKWNK2 Ensembl:ENSG00000165238 euGenes: HUgn65268
    ECgene: WNK2 H-InvDB: WNK2

    (According to HUGE)
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    HUGE: KIAA1760

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for WNK2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for WNK2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for WNK2 gene:
    Search GeneIP for patents involving WNK2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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