Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

WNK2 Gene

protein-coding GIFtS: 56
GCID: GC09P095947

WNK lysine deficient protein kinase 2

(Previous name: serologically defined colon cancer antigen 43 )
(Previous symbols: SDCCAG43, PRKWNK2)

Explore 9 diseases affiliated with
WNK2 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
WNK Lysine Deficient Protein Kinase 2¹ ²		Protein Kinase With No Lysine 2² ³
Serologically Defined Colon Cancer Antigen 43¹ ² ³		EC 2.7.11.1³ ⁸
PRKWNK2¹ ² ³		P/OKcl.13²
SDCCAG43¹ ² ³		Mitogen-Activated Protein Kinase Kinase Kinase²
NY-CO-43¹ ²		Protein Kinase, Lysine Deficient 2²
KIAA1760¹ ³		Serine/Threonine-Protein Kinase WNK2²
Antigen NY-CO-43² ³		PRK⁵
Protein Kinase Lysine-Deficient 2² ³		EC 2.7.11⁸

External Ids:	HGNC: 14542¹	Entrez Gene: 65268²	Ensembl: ENSG00000165238⁷	OMIM: 606249⁵	UniProtKB: Q9Y3S1³

Export aliases for WNK2 gene to outside databases

Previous GC identifers: GC09P093027 GC09P094987 GC09P065623

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for WNK2:

The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine

found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase

activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved

lysine. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1

Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell

signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters

and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and

inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell

cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on

phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of

the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity

Gene Wiki entry for WNK2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000009.11 NC_018920.1 NT_008470.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the WNK2 gene promoter:
C/EBPbeta RP58 AP-1 ATF-2 Lmo2 MyoD Egr-4 Evi-1 c-Jun Pax-4a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: WNK2 promoter sequence

Search SABiosciences Chromatin IP Primers for WNK2

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WNK2

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q22.3 Ensembl cytogenetic band: 9q22.31 HGNC cytogenetic band: 9q22.3

WNK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WNK2 gene location

GeneLoc information about chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09P095947: view genomic region (about GC identifiers)

Start:	95,947,198 bp from pter	End:	96,082,854 bp from pter
Size:	135,657 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1 (See protein sequence)

Recommended Name: Serine/threonine-protein kinase WNK2

Size: 2297 amino acids; 242676 Da

Cofactor: Magnesium (By similarity)

Subunit: Forms a complex with the phosphorylated form of STK39 (By similarity)

Subcellular location: Cytoplasm. Cell membrane

Caution: Cys-224 is present instead of the conserved Lys which is expected to be an active site residue. Lys-207

appears to fulfill the required catalytic function

Sequence caution: Sequence=AAH37965.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

Secondary accessions: Q5VWF1 Q5VWF2 Q8IY36 Q9C0A3 Q9H3P4

Alternative splicing: 4 isoforms: Q9Y3S1-1 Q9Y3S1-2 Q9Y3S1-3 Q9Y3S1-4 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for WNK2: NX_Q9Y3S1

Post-translational modifications:

Autophosphorylated¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q9Y3S1

4/13 DME Specific Peptides for WNK2 (Q9Y3S1) (see all 13)

EYPYSEC

IEQMKDV

YDESVDV

PPIIHRD

WNK2 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_006639.3

ENSEMBL proteins:

ENSP00000412465 ENSP00000297954 ENSP00000415038 ENSP00000414622 ENSP00000390441

ENSP00000413325 ENSP00000378860 ENSP00000378858 ENSP00000297876 ENSP00000411181

ENSP00000348347

Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant WNK2 Protein
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for WNK2
	GenScript Custom Purified and Recombinant Proteins Services for WNK2
	Novus Biologicals WNK2 Protein
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for WNK2

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IDA	17667937
GO:0005886	plasma membrane	IEA	--

WNK2 for ontologies About GeneDecksing

WNK2 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of WNK2
	Browse R&D Systems for Antibodies
	Browse OriGene Antibodies OriGene Custom Antibody Services for WNK2
	GenScript Custom Superior Antibodies Services for WNK2
	Novus Biologicals WNK2 Antibodies
	Search for Antibodies for WNK2 at Abcam
	Uscn Antibodies for WNK2
	Search ThermoFisher Antibodies for WNK2

Assay Products for WNK2:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for WNK2
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for WNK2

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

WNK2 for domains About GeneDecksing

4 InterPro domains/families:

IPR011009 Kinase-like_dom

IPR024678 Kinase_OSR1/WNK_CCT

IPR008271 Ser/Thr_kinase_AS

IPR000719 Prot_kinase_cat_dom

Graphical View of Domain Structure for InterPro Entry Q9Y3S1

ProtoNet protein and cluster: Q9Y3S1

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1

Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily

Similarity: Contains 1 protein kinase domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1

Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell

signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters

and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and

inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell

cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on

phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of

the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity

Catalytic activity: ATP + a protein = ADP + a phosphoprotein

Enzyme regulation: Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes

increased activity (By similarity)

Enzyme Numbers (IUBMB): EC 2.7.11.1¹ ² EC 2.7.11²

miRNA Products:		OriGene 3'-UTR Clone: WNK2 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat WNK2
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate WNK2
		SwitchGear 3'UTR luciferase reporter plasmid: WNK2 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WNK2 (see all 4) OriGene shRNA RFP: WNK2 OriGene siRNA: WNK2
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WNK2

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for WNK2

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2 (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: WNK2 (NM_006648)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for WNK2
		Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat WNK2

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for WNK2
		Search LifeMap BioReagents cell lines for WNK2

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNK2

Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004674	protein serine/threonine kinase activity	ISS	--
GO:0005524	ATP binding	ISS	--
GO:0016772	transferase activity, transferring phosphorus-containing groups	--	--

WNK2 for ontologies About GeneDecksing

1 GenomeRNAi human phenotype for WNK2:

Increased cell death HMECs cel

Animal Models:
Mouse knock-out Wnk2^tm1Lex for WNK2
1 MGI phenotypic allele

for Wnk2 (no phenotypes)

WNK2 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for WNK2

STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

5/8 Interacting proteins for WNK2 (Q9Y3S1^{2, 3} ENSP00000297954⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 8)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ABL1	P00519²^,³, ENSP00000361423⁴	MINT-8110875 I2D: score=3 STRING: ENSP00000361423
FYN	P06241²^,³, ENSP00000357656⁴	MINT-8111619 I2D: score=3 STRING: ENSP00000357656
ATXN1	P54253³, ENSP00000244769⁴	I2D: score=2 STRING: ENSP00000244769
UBE2I	P63279³, ENSP00000324897⁴	I2D: score=1 STRING: ENSP00000324897
YWHAZ	P63104³, ENSP00000309503⁴	I2D: score=2 STRING: ENSP00000309503

About this table

Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006468	protein phosphorylation	IDA	--
GO:0007243	intracellular protein kinase cascade	ISS	--
GO:0008285	negative regulation of cell proliferation	IMP	17667937
GO:0046777	protein autophosphorylation	IDA	17667937
GO:0070373	negative regulation of ERK1 and ERK2 cascade	IMP	17667937

WNK2 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for WNK2

3 HMDB Compounds for WNK2 About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Magnesium	Magnesium (see all 2)	7439-95-4	--

Search CenterWatch for drugs/clinical trials and news about WNK2

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for WNK2 gene:

NM_006648.3

Unigene Cluster for WNK2:

WNK lysine deficient protein kinase 2

Hs.654856 [show with all ESTs]

Unigene Representative Sequence: AB051547

18/19 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19):

ENST00000448039(uc010mrd.1) ENST00000297954(uc004ati.1) ENST00000432730(uc011lud.1)

ENST00000411624(uc004atk.3) ENST00000464625 ENST00000478583 ENST00000448251(uc004atl.1)

ENST00000462595 ENST00000453718 ENST00000474009 ENST00000479696 ENST00000467401

ENST00000460335 ENST00000471076 ENST00000395477(uc004atj.3) ENST00000395475(uc010mrc.1)

ENST00000349097 ENST00000427277

miRNA Products:		OriGene 3'-UTR Clone: WNK2 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat WNK2
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate WNK2
		SwitchGear 3'UTR luciferase reporter plasmid: WNK2 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WNK2 (see all 4) OriGene shRNA RFP: WNK2 OriGene siRNA: WNK2
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WNK2

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2 (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: WNK2 (NM_006648)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for WNK2
		Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat WNK2

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WNK2 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat WNK2
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WNK2
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WNK2

Additional cDNA sequence:

AB044546.1 AB051547.2 AJ242724.4 AK000694.1 AK055151.1 AK093910.1 AK124314.1 AK310390.1

BC037965.1

14 DOTS entries:

DT.100022041 DT.100739040 DT.91801966 DT.442052 DT.95325142 DT.425812 DT.91651393 DT.99999119

DT.40218586 DT.100016290 DT.121164654 DT.40109714 DT.92419749 DT.91789567

24/110 AceView cDNA sequences (see all 110):

AI632414 BQ222235 AW500011 BG231979 AW001397 BE561168 CA946368 AA662951

AL134251 AA283935 AW419087 BC037965 NM_006648 AB051547 AJ242724 AI217214

AA368833 BQ925250 BM671000 AW337451 AA367042 AA970341 BM703415 W73992

GeneLoc Exon Structure

5/17 Alternative Splicing Database (ASD) splice patterns (SP) for WNK2 (see all 17) About this scheme

ExUns:	1	^	2	^	3	^	4a	·	4b	·	4c	^	5	^	6	^	7a	·	7b	^	8	^	9	^	10	^	11	^	12a	·	12b	·	12c	^	13	^	14	^	15a	·	15b	^	16a	·	16b	^	17	^	18a	·	18b	^
SP1:																																															-
SP2:							-																-
SP3:																																															-
SP4:
SP5:																																					-										-

ExUns:	19	^	20	^	21a	·	21b	^	22a	·	22b	·	22c	^	23	^	24	^	25	^	26a	·	26b	^	27	^	28	^	29	^	30	^	31a	·	31b	·	31c	^	32a	·	32b	^	33	^	34	^	35
SP1:					-																												-						-				-		-
SP2:
SP3:					-
SP4:
SP5:

ECgene alternative splicing isoforms for WNK2

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

WNK2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTGGTCAGTG

About this image

WNK2 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Pancreatic endoderm/endocrine precursor-like cells (A scalable, suspensi...)

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See WNK2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for WNK2

SOURCE GeneReport for Unigene cluster: Hs.654856

UniProtKB/Swiss-Prot: WNK2_HUMAN, Q9Y3S1

Tissue specificity: Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain,

skeletal muscle and colon

SABiosciences Custom PCR Arrays for WNK2

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WNK2 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat WNK2
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WNK2
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WNK2

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNK2

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for WNK2 gene from 7/23 species (see all 23) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	WNK2¹	WNK lysine deficient protein kinase 2	69.94(n) 73.74(a)		415984 XM_414326.3 XP_414326.3
lizard (Anolis carolinensis)	Reptilia	WNK2⁶ --	--	53(a) 13(a)	1 ↔ 1 possible ortholog	GL343225.1(1588925-1672311) 1(205439218-205490633)
zebrafish (Danio rerio)	Actinopterygii	CABZ01066573.1⁶	--	71(a)	1 ↔ 1	25(1497582-1507054)
fruit fly (Drosophila melanogaster)	Insecta	Wnk⁶	--	15(a)	1 → many	3L(21536950-21548028)
worm (Caenorhabditis elegans)	Secernentea	wnk-1⁶	mammalian WNK-type protein kinase homolog family m...	20(a)	1 → many	IV(9200100-9212216)
thale cress (Arabidopsis thaliana)	eudicotyledons	AT5G55560⁶ WNK3⁶ (see all 11)	putative serine/threonine-protein kinase WNK3 (see all 11)	40(a) 33(a) (see all 11)	many ↔ many many ↔ many (see all 11)	5(22506302-22507879) 3(17873012-17875220)
rice (Oryza sativa)	Liliopsida	Os.2221²	Oryza sativa mitogen activated protein kinase kinase more	77.64(n)		AK100426.1

ENSEMBL Gene Tree for WNK2 (if available)
TreeFam Gene Tree for WNK2 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for WNK2 gene

WNK1² WNK4² NRBP1² NRBP2² WNK3²

7 SIMAP similar genes for WNK2 using alignment to 11 protein entries: WNK2_HUMAN (see all proteins):

WNK3 STK11 WNK4 NEK6 CDK4 STK17A

WNK1

WNK2 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 9 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs138695382^1,2	C,F	other	96061517(+)	`AGCCAG/ATGATG`	2	/N /S	mis¹		2		NA EU	5047
rs147169141^1,2	C,	--	95945238(+)	`CAGGC-/TACAGG`	1	--	us2k¹		0	--	--	--	--
rs190937585^1,2		--	95945327(+)	`CCAGCA/GGGGCT`	1	--	us2k¹		0	--	--	--	--
rs76786528^1,2	C,	--	95945359(+)	`GGTAAC/GAGTTG`	1	--	us2k¹		2		NA	122
rs151254187^1,2		--	95945759(+)	`AGAGAA/TCATGG`	1	--	us2k¹		0	--	--	--	--
rs182832047^1,2		--	95945976(+)	`GCCTCC/TCTGGG`	1	--	us2k¹		0	--	--	--	--
rs140424063^1,2		--	95946067(+)	`GGCTGC/GGCTCC`	1	--	us2k¹		0	--	--	--	--
rs114883971^1,2	F,	--	95946153(+)	`CGCGCC/TGGGCT`	1	--	us2k¹		1		WA	118
rs75476871^1,2	C,	--	95946232(+)	`CCCTGA/CGGGGC`	1	--	us2k¹		0	--	--	--	--
rs7045686^1,2	C,	--	95946332(+)	`GCGCCC/TGCCAG`	1	--	us2k¹		6		NA CSA	129

HapMap Linkage Disequilibrium report for WNK2 (95947198 - 96082854 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 6 variations for WNK2
2 CNVs: 96192 96193
4 Indels: 13182 100713 96194 13183

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing WNK2

DNA2.0 Custom Variant and Variant Library Synthesis for WNK2

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

WNK2 for disorders           About GeneDecksing

OMIM gene information: 606249    OMIM disorders: --

9 diseases for WNK2:    About MalaCards

colon cancer pseudohypoaldosteronism type ii pseudohypoaldosteronism kidney disease

pancreatic cancer pancreatitis ataxia hypertension

neuronitis

1 disease from the University of Copenhagen DISEASES database for WNK2:

Pseudohypoaldosteronism

Human Genome Epidemiology (HuGE) Navigator: WNK2 (4 documents)

Export disorders for WNK2 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for WNK2 gene, integrated from 9 sources (see all 28):
(articles sorted by number of sources associating them with WNK2)

Utopia: connect your pdf to the dynamic
world of online information

WNK kinases, a novel protein kinase subfamily in multi-cellular organisms. (PubMed id 11571656)^{1, 2, 3} Verissimo F. and Jordan P. (2001)
WNK2 modulates MEK1 activity through the Rho GTPase pathway. (PubMed id 18593598)^{1, 2, 9} Moniz S....Jordan P. (2008)
WNK2 is a novel regulator of essential neuronal cation-chloride cotransporters. (PubMed id 21733846)^{1, 2} Rinehart J.... Lifton R.P. (2011)
Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2. (PubMed id 17667937)^{1, 2} Moniz S....Jordan P. (2007)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Molecular basis of T cell-mediated recognition of pancreatic cancer cells. (PubMed id 11280764)^{1, 2} Ito M....Itoh K. (2001)
Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 11214970)^{1, 2} Nagase T.... Ohara O. (2000)
Characterization of human colon cancer antigens recognized by autologous antibodies. (PubMed id 9610721)^{1, 3} Scanlan M.J....Old L.J. (1998)
Epigenome scans and cancer genome sequencing converge on WNK2, a kinase-independent suppressor of cell growth. (PubMed id 17578925)^{1, 9} Hong C....Costello J.F. (2007)
System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)² Rigbolt K.T....Blagoev B. (2011)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 65268	HGNC: 14542	AceView: PRKWNK2	Ensembl:ENSG00000165238	euGenes: HUgn65268
ECgene: WNK2	H-InvDB: WNK2

(According to HUGE)
About This Section

HUGE: KIAA1760

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for WNK2	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for WNK2	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for WNK2 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for WNK2 gene:
Search GeneIP for patents involving WNK2

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

	Browse Small Molecules at EMD Millipore
	Browse for Gene Knock-down Tools from EMD Millipore
	Browse Kits and Assays available from EMD Millipore
	Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
	EMD Millipore Mono- and Polyclonal Antibodies for the study of WNK2
	EMD Millipore Purified and/or Recombinant WNK2 Protein

	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	Browse OriGene Antibodies		OriGene shRNA RFP for WNK2
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WNK2		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WNK2
	Browse OriGene Protein Over-expression Lysates		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for WNK2		OriGene 3'-UTR Clone for WNK2
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WNK2
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for WNK2		OriGene Custom Protein Services for WNK2
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat WNK2
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing WNK2
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WNK2
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WNK2
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WNK2
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WNK2

	GenScript Custom Purified and Recombinant Proteins Services for WNK2		GenScript cDNA clones with any tag delivered in your preferred vector for WNK2
	GenScript Custom Assay Services for WNK2		GenScript Custom Superior Antibodies Services for WNK2
	GenScript Custom overexpressing Cell Line Services for WNK2		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Regulatory tfbs in WNK2 promoter
	Search Chromatin IP Primers for WNK2
	RT² qPCR Primer Assay in human, mouse, rat WNK2
	GNC Network for WNK2
	SABiosciences Custom PCR Arrays for WNK2

Search Tocris compounds for WNK2

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	WNK2 antibodies
	WNK2 proteins

	Search for Antibodies for WNK2 at Abcam
	See all of Abcam's Antibodies, Kits and Proteins for WNK2
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

WNK2 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for WNK2

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WNK2

	SwitchGear 3'UTR luciferase reporter plasmids for WNK2
	SwitchGear Promoter luciferase reporter plasmids for WNK2

Search ThermoFisher Antibodies for WNK2

Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat WNK2

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes WNK2 gene at Home site.

hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

WNK2 Gene

WNK lysine deficient protein kinase 2

Aliases
for WNK2 gene

Summaries
for WNK2 gene

Genomic Views
for WNK2 gene

Proteins
for WNK2 gene

Protein Domains /
Families
for WNK2 gene

Function
for WNK2 gene

Pathways & Interactions
for WNK2 gene

Drugs & Compounds
for WNK2 gene

Transcripts
for WNK2 gene

Expression
for WNK2 gene

Orthologs
for WNK2 gene

Paralogs
for WNK2 gene

Genomic Variants
for WNK2 gene

Disorders / Diseases
for WNK2 gene

Publications
for WNK2 gene

External Searches for WNK2 gene

Genome Databases showing WNK2 gene

Other Databases showing WNK2 gene

Specialized Databases showing WNK2 gene

Intellectual Property
for WNK2 gene

Products
for WNK2 gene

View Random Gene

Advanced Search

Export list of displayed genes

WNK2 Gene

WNK lysine deficient protein kinase 2

Aliasesfor WNK2 gene

Summariesfor WNK2 gene

Genomic Viewsfor WNK2 gene

Proteinsfor WNK2 gene

Protein Domains /Familiesfor WNK2 gene

Functionfor WNK2 gene

Pathways & Interactionsfor WNK2 gene

Drugs & Compoundsfor WNK2 gene

Transcriptsfor WNK2 gene

Expression for WNK2 gene

Orthologsfor WNK2 gene

Paralogsfor WNK2 gene

Genomic Variantsfor WNK2 gene

Disorders / Diseasesfor WNK2 gene

Publicationsfor WNK2 gene

External Searches for WNK2 gene

Genome Databases showing WNK2 gene

Other Databases showing WNK2 gene

Specialized Databases showing WNK2 gene

Intellectual Propertyfor WNK2 gene

Productsfor WNK2 gene

View Random Gene

Aliases
for WNK2 gene

Summaries
for WNK2 gene

Genomic Views
for WNK2 gene

Proteins
for WNK2 gene

Protein Domains /
Families
for WNK2 gene

Function
for WNK2 gene

Pathways & Interactions
for WNK2 gene

Drugs & Compounds
for WNK2 gene

Transcripts
for WNK2 gene

Expression
for WNK2 gene

Orthologs
for WNK2 gene

Paralogs
for WNK2 gene

Genomic Variants
for WNK2 gene

Disorders / Diseases
for WNK2 gene

Publications
for WNK2 gene

Intellectual Property
for WNK2 gene

Products
for WNK2 gene