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WFDC10B Gene

protein-coding   GIFtS: 40
GCID: GC20M044314

WAP Four-Disulfide Core Domain 10B

  Search for WFDC10B
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
WAP Four-Disulfide Core Domain 10B1 2
WAP122 3
Protease Inhibitor WAP122
Protein WFDC10B2

External Ids:    HGNC: 204791   Entrez Gene: 2806642   Ensembl: ENSG000001829317   UniProtKB: Q8IUB33   

Export aliases for WFDC10B gene to outside databases

Previous GC identifers: GC20U900001 GC20M044951 GC20M044998 GC20M043746 GC20M041054


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for WFDC10B Gene:
This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP
signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions
as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters:
centromeric and telomeric. This gene belongs to the telomeric cluster. Two alternatively spliced transcript
variants have been found for this gene, and they encode distinct isoforms. (provided by RefSeq, Jul 2008)

GeneCards Summary for WFDC10B Gene:
WFDC10B (WAP four-disulfide core domain 10B) is a protein-coding gene. GO annotations related to this gene include peptidase inhibitor activity.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000020.11  NC_018931.2  NT_011362.11  
Regulatory elements:
   Regulatory transcription factor binding sites in the WFDC10B gene promoter:
         AhR   RFX1   Pax-6   Arnt   Gfi-1   MEF-2A   aMEF-2   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidWFDC10B promoter sequence
   Search Chromatin IP Primers for WFDC10B

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WFDC10B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.12   Ensembl cytogenetic band:  20q13.12   HGNC cytogenetic band: 20q13.12

WFDC10B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WFDC10B gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M044314:  view genomic region     (about GC identifiers)

Start:
44,313,290 bp from pter      End:
44,333,658 bp from pter
Size:
20,369 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: WF10B_HUMAN, Q8IUB3 (See protein sequence)
Recommended Name: Protein WFDC10B precursor  
Size: 73 amino acids; 8325 Da
Secondary accessions: A6PVD7 Q0VAG0 Q0VAG1 Q5TGZ5 Q8IUB4
Alternative splicing: 2 isoforms:  Q8IUB3-1   Q8IUB3-2   

Explore the universe of human proteins at neXtProt for WFDC10B: NX_Q8IUB3

Explore proteomics data for WFDC10B at MOPED


See WFDC10B Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (2 alternative transcripts): 
NP_742003.1  NP_742143.1  

ENSEMBL proteins: 
 ENSP00000327628   ENSP00000337466  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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HGNC Gene Families:
WFDC: WAP four-disulfide core domain containing

1 InterPro protein domain:
 IPR008197 WAP-type_4-diS_core

Graphical View of Domain Structure for InterPro Entry Q8IUB3

ProtoNet protein and cluster: Q8IUB3

1 Blocks protein domain: IPB008197 4-disulphide core signature

UniProtKB/Swiss-Prot: WF10B_HUMAN, Q8IUB3
Similarity: Contains 1 WAP domain


WFDC10B for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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     Gene Ontology (GO): 1 molecular function term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0030414peptidase inhibitor activity IEA--
     
WFDC10B for ontologies           About GeneDecksing


Phenotypes:
     1 GenomeRNAi human phenotype for WFDC10B:
 Cells with protrusions 

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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
WF10B_HUMAN, Q8IUB3: Secreted (Potential)
Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
extracellular3
cytosol2
nucleus2

Gene Ontology (GO): 1 cellular component term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005576extracellular region IEA--

WFDC10B for ontologies           About GeneDecksing


(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for WFDC10B
Interactions:

    Search GeneGlobe Interaction Network for WFDC10B

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for WFDC10B (WF10B)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for WFDC10B gene (2 alternative transcripts): 
NM_172006.2  NM_172131.2  

Unigene Cluster for WFDC10B:

WAP four-disulfide core domain 10B
Hs.237392  [show with all ESTs]
Unigene Representative Sequence: NM_172006
2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000330523(uc002xpb.3) ENST00000335769(uc002xpc.3)
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Additional mRNA sequence: 

AF454506.1 AF454507.1 BC121071.1 BC121072.1 

3 DOTS entries:

DT.100015336  DT.100015335  DT.65286404 

6 AceView cDNA sequences:

NM_172131 NM_172006 AF454506 AF454507 BV192880 BI035811 

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for WFDC10B    About this scheme

ExUns: 1 ^ 2 ^ 3 ^ 4
SP1:                        
SP2:              -         


ECgene alternative splicing isoforms for WFDC10B

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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WFDC10B expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: --
WFDC10B Expression
About this image

WFDC10B Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

WFDC10B Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.237392

UniProtKB/Swiss-Prot: WF10B_HUMAN, Q8IUB3
Tissue specificity: Ubiquitously expressed

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(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of human and chimp.

Orthologs for WFDC10B gene from 1 species (see representative species )    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
chimpanzee
(Pan troglodytes)
Mammalia WFDC10B6
WAP four-disulfide core domain 10B
98(a)
1 ↔ 1
20(42673039-42701246)


ENSEMBL Gene Tree for WFDC10B (if available)
TreeFam Gene Tree for WFDC10B (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for WFDC10B gene
3 SIMAP similar genes for WFDC10B using alignment to 1 protein entry:     WF10B_HUMAN:
WFDC10A    WFDC11    WFDC9

WFDC10B for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for WFDC10B (see all 366)    About this table    
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 20 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs22812111,2
C,F,A,H--44312858(+) ATTATC/TCTCAT 2 -- ds500116Minor allele frequency- T:0.23NS EA NA WA 1150
rs801530091,2
C,F--44312929(+) CCAAGG/ACTAAG 2 -- ds50011Minor allele frequency- A:0.03WA 118
rs1863346281,2
--44312950(+) CAGTGC/TTTGGG 2 -- ds50010--------
rs1910322951,2
--44312957(+) TGGGGC/GCCTCC 2 -- ds50010--------
rs18257751,2
C,F,O,A,H--44312991(+) TGCCAC/TCCATC 2 -- ds500110Minor allele frequency- N:0.00EA NA WA 584
rs1465248131,2
--44312992(+) GCCACC/TCATCT 2 -- ds50010--------
rs60738561,2
C,F,A,H--44313020(+) TCTGAT/CTCCAG 2 -- ds50018Minor allele frequency- C:0.47NA WA CSA 19
rs1153527811,2
C,F--44313058(+) GAGACG/ACTGCA 2 -- ds50011Minor allele frequency- A:0.03WA 118
rs1436628021,2
--44313117(+) CAAGGC/GGCTGG 2 -- ds50010--------
rs1160481271,2
C,F--44313160(+) AAAGAA/GGGAGA 2 -- ds50011Minor allele frequency- G:0.03WA 118

HapMap Linkage Disequilibrium report for WFDC10B (44313290 - 44333658 bp)

Structural Variations
     Database of Genomic Variants (DGV) 1 variation for WFDC10B:    About this table    
Variant IDTypeSubtypePubMed ID
nsv525287CNV Loss19592680

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing WFDC10B
DNA2.0 Custom Variant and Variant Library Synthesis for WFDC10B

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for WFDC10B gene integrated from 10 sources:
(articles sorted by number of sources associating them with WFDC10B)
    Utopia: connect your pdf to the dynamic
world of online information

  1. A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. (PubMed id 12206714)1, 2, 3 Clauss A.... Lundwall A. (Biochem. J. 2002)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  3. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (Nature 2001)
  4. The evolution of a genetic locus encoding small serine proteinase inhibitors. (PubMed id 15950183)1 Clauss A....Lundwall A. (Biochem. Biophys. Res. Commun. 2005)
  5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 280664 HGNC: 20479 AceView: WFDC10B Ensembl:ENSG00000182931 euGenes: HUgn280664
ECgene: WFDC10B H-InvDB: WFDC10B

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for WFDC10B Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for WFDC10B gene:
Search GeneIP for patents involving WFDC10B

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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 CLIAs for WFDC10B



 Browse ESI BIO Cell Lines and PureStem Progenitors for WFDC10B
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WFDC10B
 SwitchGear 3'UTR luciferase reporter plasmids for WFDC10B
 SwitchGear Promoter luciferase reporter plasmids for WFDC10B
 Search ThermoFisher Antibodies for WFDC10B
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WFDC10B
 inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for WFDC10B
 inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for WFDC10B
 Search LSBio for Antibodies for WFDC10B
Customized transgenic rodents for:
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GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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