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WFDC10A Gene

protein-coding   GIFtS: 37
GCID: GC20P044258

WAP Four-Disulfide Core Domain 10A

(Previous name: chromosome 20 open reading frame 146)
(Previous symbol: C20orf146)
  Search for WFDC10A
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
WAP Four-Disulfide Core Domain 10A1 2     Chromosome 20 Open Reading Frame 1461
C20orf1461 2 3     dJ688G8.32
Putative Protease Inhibitor WAP10A2 3     WAP Four-Disulfide Core Domain Protein 10A2
WAP102 3     

External Ids:    HGNC: 161391   Entrez Gene: 1408322   Ensembl: ENSG000001803057   UniProtKB: Q9H1F03   

Export aliases for WFDC10A gene to outside databases

Previous GC identifers: GC20P043946 GC20P044896 GC20P044943 GC20P043691 GC20P040999


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for WFDC10A Gene:
This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP
signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions
as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters:
centromeric and telomeric. This gene belongs to the telomeric cluster. (provided by RefSeq, Jul 2008)

GeneCards Summary for WFDC10A Gene:
WFDC10A (WAP four-disulfide core domain 10A) is a protein-coding gene. GO annotations related to this gene include serine-type endopeptidase inhibitor activity.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000020.11  NC_018931.2  NT_011362.11  
Regulatory elements:
   Regulatory transcription factor binding sites in the WFDC10A gene promoter:
         Ik-2   HNF-1   STAT3   Nkx5-1   HNF-3beta   HNF-1A   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidWFDC10A promoter sequence
   Search Chromatin IP Primers for WFDC10A

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WFDC10A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.12   Ensembl cytogenetic band:  20q13.12   HGNC cytogenetic band: 20q13.11

WFDC10A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WFDC10A gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P044258:  view genomic region     (about GC identifiers)

Start:
44,258,165 bp from pter      End:
44,259,835 bp from pter
Size:
1,671 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: WF10A_HUMAN, Q9H1F0 (See protein sequence)
Recommended Name: WAP four-disulfide core domain protein 10A precursor  
Size: 79 amino acids; 8943 Da
Secondary accessions: A2RRE9 Q5TGZ7

Explore the universe of human proteins at neXtProt for WFDC10A: NX_Q9H1F0

Explore proteomics data for WFDC10A at MOPED


See WFDC10A Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_542791.1  
ENSEMBL proteins: 
 ENSP00000361726  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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HGNC Gene Families:
WFDC: WAP four-disulfide core domain containing

1 InterPro protein domain:
 IPR008197 WAP-type_4-diS_core

Graphical View of Domain Structure for InterPro Entry Q9H1F0

ProtoNet protein and cluster: Q9H1F0

1 Blocks protein domain: IPB008197 4-disulphide core signature

UniProtKB/Swiss-Prot: WF10A_HUMAN, Q9H1F0
Similarity: Contains 1 WAP domain


WFDC10A for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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     Gene Ontology (GO): 2 molecular function terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0004867serine-type endopeptidase inhibitor activity IEA--
GO:0030414peptidase inhibitor activity ----
     
WFDC10A for ontologies           About GeneDecksing


Phenotypes:
     1 GenomeRNAi human phenotype for WFDC10A:
 Decreased cilium length after  

Animal Models:
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hsa-miR-299-5p hsa-miR-1324 hsa-miR-29b-1*
SwitchGear 3'UTR luciferase reporter plasmidWFDC10A 3' UTR sequence
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
WF10A_HUMAN, Q9H1F0: Secreted (Potential)
Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
extracellular3

Gene Ontology (GO): 1 cellular component term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005576extracellular region IEA--

WFDC10A for ontologies           About GeneDecksing


(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for WFDC10A
Interactions:

    Search GeneGlobe Interaction Network for WFDC10A

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Tocris compounds for WFDC10A (WF10A)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for WFDC10A gene: 
NM_080753.2  

Unigene Cluster for WFDC10A:

WAP four-disulfide core domain 10A
Hs.199380  [show with all ESTs]
Unigene Representative Sequence: BG194425
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000372643(uc002xoz.3)
miRNA
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hsa-miR-299-5p hsa-miR-1324 hsa-miR-29b-1*
SwitchGear 3'UTR luciferase reporter plasmidWFDC10A 3' UTR sequence
Inhib. RNA
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  QuantiFast Probe-based Assays in human, mouse, rat WFDC10A

Additional mRNA sequence: 

AY038182.1 BC131579.1 

1 DOTS entry:

DT.100000412 

7 AceView cDNA sequences:

CR598861 AY038182 NM_080753 AI673291 BX380556 BX330465 BG181924 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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WFDC10A expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: AGCATCCTGT
WFDC10A Expression
About this image

WFDC10A Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

WFDC10A Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.199380
    Custom PCR Arrays for WFDC10A
Primer
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In Situ
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(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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  --

ENSEMBL Gene Tree for WFDC10A (if available)
TreeFam Gene Tree for WFDC10A (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for WFDC10A gene
2 SIMAP similar genes for WFDC10A using alignment to 1 protein entry:     WF10A_HUMAN:
WFDC10B    WFDC11

WFDC10A for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Structural Variations
     Database of Genomic Variants (DGV) 2 variations for WFDC10A:    About this table    
Variant IDTypeSubtypePubMed ID
nsv912893CNV Loss21882294
nsv7342OTHER Inversion18451855

Site Specific Mutation Identification with PCR Assays
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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for WFDC10A gene integrated from 10 sources:
(articles sorted by number of sources associating them with WFDC10A)
    Utopia: connect your pdf to the dynamic
world of online information

  1. A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. (PubMed id 12206714)1, 2, 3 Clauss A.... Lundwall A. (Biochem. J. 2002)
  2. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (Nature 2001)
  3. The evolution of a genetic locus encoding small serine proteinase inhibitors. (PubMed id 15950183)1 Clauss A....Lundwall A. (Biochem. Biophys. Res. Commun. 2005)
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 140832 HGNC: 16139 AceView: WFDC10A Ensembl:ENSG00000180305 euGenes: HUgn140832
ECgene: WFDC10A H-InvDB: WFDC10A

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for WFDC10A Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for WFDC10A gene:
Search GeneIP for patents involving WFDC10A

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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