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Aliases for WEE1 Gene

Aliases for WEE1 Gene

  • WEE1 G2 Checkpoint Kinase 2 3 5
  • Wee1A Kinase 3 4
  • EC 2.7.10.2 4 61
  • WEE1hu 3 4
  • Wee1+ (S. Pombe) Homolog 2
  • Wee1-Like Protein Kinase 3
  • WEE1 Homolog (S. Pombe) 2
  • WEE1+ Homolog 3
  • WEE1 Homolog 3
  • WEE1A 3

External Ids for WEE1 Gene

Previous GeneCards Identifiers for WEE1 Gene

  • GC11P010605
  • GC11P010164
  • GC11P009633
  • GC11P009558
  • GC11P009550
  • GC11P009266
  • GC11P009595

Summaries for WEE1 Gene

Entrez Gene Summary for WEE1 Gene

  • This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

CIViC summary for WEE1 Gene

GeneCards Summary for WEE1 Gene

WEE1 (WEE1 G2 Checkpoint Kinase) is a Protein Coding gene. Among its related pathways are PLK1 signaling events and Cell cycle Role of 14-3-3 proteins in cell cycle regulation. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is WEE2.

UniProtKB/Swiss-Prot for WEE1 Gene

  • Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on Tyr-15. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on Tyr-15 and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gene Wiki entry for WEE1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for WEE1 Gene

Genomics for WEE1 Gene

Regulatory Elements for WEE1 Gene

Enhancers for WEE1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH11G009611 2.1 FANTOM5 Ensembl ENCODE dbSUPER 16.7 +42.4 42424 8.5 MLX CREB3L1 ZFP64 FEZF1 DMAP1 YY1 ZNF143 ZNF263 SP3 TBX21 SWAP70 WEE1 RPL21P97 ENSG00000238387 ZNF143 DENND5A GC11M009621 GC11M009608
GH11G009565 1.9 FANTOM5 ENCODE dbSUPER 11.9 -1.4 -1403 15.2 MLX CREB3L1 ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF263 SP3 NFYC SWAP70 WEE1 RPL21P97 AKIP1 ZNF143 TMEM41B DENND5A PIR40365
GH11G009748 1.7 FANTOM5 Ensembl ENCODE dbSUPER 13 +175.0 174950 1.1 HDAC1 HDGF SIN3A ARID4B NFXL1 RAD21 GLIS2 ZNF143 ZNF207 RCOR1 SWAP70 WEE1 RPL21P97 ENSG00000238387 GC11P009745 PIR37097
GH11G009551 1.7 Ensembl ENCODE dbSUPER 12.5 -21.4 -21388 1.8 CREB3L1 MLX ZFP64 DMAP1 YY1 SLC30A9 ZNF263 SP3 NFYC MEF2D SNORA3B WEE1 ENSG00000238387 RPL27A RPL21P97 AKIP1 ZNF143 PIR40365 LOC644656
GH11G009751 2 FANTOM5 Ensembl ENCODE dbSUPER 10.3 +186.1 186119 15.9 MLX CREB3L1 ZFP64 FEZF1 DMAP1 SLC30A9 ZNF143 ZNF263 SP3 GLIS1 SBF2 SWAP70 TMEM9B-AS1 ENSG00000254397 RPL21P97 SBF2-AS1 ENSG00000238387 WEE1 CTR9 NRIP3
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around WEE1 on UCSC Golden Path with GeneCards custom track

Promoters for WEE1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000036604 1350 4801 HNRNPUL1 FOXA2 MLX CREB3L1 ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1

Transcription factor binding sites by QIAGEN in the WEE1 gene promoter:

Genomic Location for WEE1 Gene

Chromosome:
11
Start:
9,573,650 bp from pter
End:
9,593,458 bp from pter
Size:
19,809 bases
Orientation:
Plus strand

Genomic View for WEE1 Gene

Genes around WEE1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
WEE1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for WEE1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for WEE1 Gene

Proteins for WEE1 Gene

  • Protein details for WEE1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P30291-WEE1_HUMAN
    Recommended name:
    Wee1-like protein kinase
    Protein Accession:
    P30291
    Secondary Accessions:
    • B3KVE1
    • D3DQV0

    Protein attributes for WEE1 Gene

    Size:
    646 amino acids
    Molecular mass:
    71597 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for WEE1 Gene

    Alternative splice isoforms for WEE1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for WEE1 Gene

Selected DME Specific Peptides for WEE1 Gene

P30291:
  • WAEDDHM
  • RLDGCIYAIK
  • YAIKRSKKPLAGS
  • GEFGSVFKCVKRLDGC
  • VEEGDSRFLANE

Post-translational modifications for WEE1 Gene

  • Dephosphorylated at Thr-239 by CTDP1.
  • Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
  • Ubiquitinated and degraded at the onset of G2/M phase.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for WEE1 Gene

Domains & Families for WEE1 Gene

Gene Families for WEE1 Gene

Graphical View of Domain Structure for InterPro Entry

P30291

UniProtKB/Swiss-Prot:

WEE1_HUMAN :
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
genes like me logo Genes that share domains with WEE1: view

Function for WEE1 Gene

Molecular function for WEE1 Gene

GENATLAS Biochemistry:
wee1 tyrosine kinase,yeast wee1 homolog,involved in cell cycle regulation
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.
UniProtKB/Swiss-Prot Function:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on Tyr-15. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on Tyr-15 and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Enzyme Numbers (IUBMB) for WEE1 Gene

Gene Ontology (GO) - Molecular Function for WEE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0000287 magnesium ion binding IEA --
GO:0004672 protein kinase activity IEA --
GO:0004713 protein tyrosine kinase activity TAS 7774574
GO:0004715 non-membrane spanning protein tyrosine kinase activity IEA --
genes like me logo Genes that share ontologies with WEE1: view
genes like me logo Genes that share phenotypes with WEE1: view

Animal Models for WEE1 Gene

MGI Knock Outs for WEE1:
  • Wee1 tm1b(EUCOMM)Wtsi

Animal Model Products

  • Taconic Biosciences Mouse Models for WEE1

miRNA for WEE1 Gene

miRTarBase miRNAs that target WEE1

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for WEE1 Gene

Localization for WEE1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for WEE1 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for WEE1 gene
Compartment Confidence
nucleus 5
cytoskeleton 2
mitochondrion 2
cytosol 2
plasma membrane 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for WEE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 8348613
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with WEE1: view

Pathways & Interactions for WEE1 Gene

genes like me logo Genes that share pathways with WEE1: view

SIGNOR curated interactions for WEE1 Gene

Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for WEE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000086 G2/M transition of mitotic cell cycle TAS --
GO:0000226 microtubule cytoskeleton organization IEA --
GO:0006468 protein phosphorylation IEA --
GO:0007049 cell cycle IEA --
genes like me logo Genes that share ontologies with WEE1: view

Drugs & Compounds for WEE1 Gene

(15) Drugs for WEE1 Gene - From: DrugBank, ApexBio, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Resveratrol Experimental, Investigational Pharma inhibitor Wee1 kinase inhibtor,potent and ATP-competitive, Others, Cyclooxygenase inhibitor 122
3-(9-HYDROXY-1,3-DIOXO-4-PHENYL-2,3-DIHYDROPYRROLO[3,4-C]CARBAZOL-6(1H)-YL)PROPANOIC ACID Experimental Pharma Target 0
4-(2-chlorophenyl)-8-(2-hydroxyethyl)-6-methylpyrrolo[3,4-e]indole-1,3(2H,6H)-dione Experimental Pharma Target 0
8-bromo-4-(2-chlorophenyl)-N-(2-hydroxyethyl)-6-methyl-1,3-dioxo-1,2,3,6-tetrahydropyrrolo[3,4-e]indole-7-carboxamide Experimental Pharma Target 0

(1) Additional Compounds for WEE1 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0

(1) ApexBio Compounds for WEE1 Gene

Compound Action Cas Number
MK-1775 Wee1 kinase inhibtor,potent and ATP-competitive 955365-80-7
genes like me logo Genes that share compounds with WEE1: view

Drug Products

Transcripts for WEE1 Gene

Unigene Clusters for WEE1 Gene

WEE1 homolog (S. pombe):
Representative Sequences:

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for WEE1 Gene

No ASD Table

Relevant External Links for WEE1 Gene

GeneLoc Exon Structure for
WEE1
ECgene alternative splicing isoforms for
WEE1

Expression for WEE1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for WEE1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for WEE1 Gene

This gene is overexpressed in Heart (66.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for WEE1 Gene



Protein tissue co-expression partners for WEE1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of WEE1 Gene:

WEE1

SOURCE GeneReport for Unigene cluster for WEE1 Gene:

Hs.249441

Evidence on tissue expression from TISSUES for WEE1 Gene

  • Nervous system(4.4)
  • Blood(4.3)
  • Intestine(2.5)
  • Liver(2.2)
genes like me logo Genes that share expression patterns with WEE1: view

Primer Products

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for WEE1 Gene

Orthologs for WEE1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for WEE1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia WEE1 34 35
  • 99.17 (n)
cow
(Bos Taurus)
Mammalia WEE1 35
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia WEE1 34 35
  • 93.24 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia WEE1 35
  • 89 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Wee1 34
  • 88.35 (n)
mouse
(Mus musculus)
Mammalia Wee1 34 16 35
  • 87.06 (n)
oppossum
(Monodelphis domestica)
Mammalia WEE1 35
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves WEE1 34 35
  • 77.05 (n)
lizard
(Anolis carolinensis)
Reptilia WEE1 35
  • 74 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.5859 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.17393 34
zebrafish
(Danio rerio)
Actinopterygii wee1 34
  • 65.76 (n)
Dr.28346 34
fruit fly
(Drosophila melanogaster)
Insecta wee 36 34 35
  • 51.51 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007798 34
  • 26.82 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SWE1 35
  • 13 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7177 35
  • 43 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3882 34
Species where no ortholog for WEE1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for WEE1 Gene

ENSEMBL:
Gene Tree for WEE1 (if available)
TreeFam:
Gene Tree for WEE1 (if available)

Paralogs for WEE1 Gene

Paralogs for WEE1 Gene

(2) SIMAP similar genes for WEE1 Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with WEE1: view

Variants for WEE1 Gene

Sequence variations from dbSNP and Humsavar for WEE1 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type
rs1000025321 -- 9,577,851(+) CAGGC(A/G)TGTAT intron-variant
rs1000062488 -- 9,576,655(+) CTGTT(A/G)ATGAG reference, missense
rs1000202111 -- 9,583,965(+) CTCAG(C/T)CTCCC intron-variant
rs1000249893 -- 9,572,126(+) GGTGA(A/C)ACTCC upstream-variant-2KB
rs1000435073 -- 9,577,053(+) CAAAA(A/G)TTTAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for WEE1 Gene

Variant ID Type Subtype PubMed ID
esv2744094 CNV deletion 23290073
nsv1069562 CNV deletion 25765185
nsv1113358 CNV deletion 24896259
nsv1143533 CNV deletion 24896259
nsv499766 OTHER inversion 21111241
nsv553467 CNV loss 21841781
nsv7215 OTHER inversion 18451855
nsv7672 CNV insertion 18451855

Variation tolerance for WEE1 Gene

Residual Variation Intolerance Score: 32.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.78; 47.24% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for WEE1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
WEE1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for WEE1 Gene

Disorders for WEE1 Gene

Relevant External Links for WEE1

Genetic Association Database (GAD)
WEE1
Human Genome Epidemiology (HuGE) Navigator
WEE1
Tumor Gene Database (TGDB):
WEE1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
WEE1

No disorders were found for WEE1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for WEE1 Gene

Publications for WEE1 Gene

  1. Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. (PMID: 15837193) Squire C.J. … Baker E.N. (Structure 2005) 3 4 22 64
  2. M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP. (PMID: 15070733) Watanabe N. … Osada H. (Proc. Natl. Acad. Sci. U.S.A. 2004) 3 4 22 64
  3. Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1). (PMID: 11528126) Cichutek A. … Zabel B.U. (Cytogenet. Cell Genet. 2001) 3 4 22 64
  4. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. (PMID: 7743995) Watanabe N. … Hunter T. (EMBO J. 1995) 3 4 22 64
  5. Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15. (PMID: 8428596) McGowan C.H. … Russell P. (EMBO J. 1993) 3 4 22 64

Products for WEE1 Gene

Sources for WEE1 Gene

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