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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

WEE1 Gene

protein-coding   GIFtS: 68
GCID: GC11P009595

WEE1 homolog (S. pombe)

(Previous name: wee1+ (S. pombe) homolog )
 Explore 27 diseases affiliated with
WEE1 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for WEE1    

Aliases
for WEE1 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
WEE1 Homolog (S. Pombe)1 2     Wee1+ (S. Pombe) Homolog1
Wee1A Kinase2 3     WEE1A2
WEE1hu2 3     WEE1+ Homolog2
EC 2.7.10.23 8     Wee1-Like Protein Kinase2

External Ids:    HGNC: 127611   Entrez Gene: 74652   Ensembl: ENSG000001664837   OMIM: 1935255   UniProtKB: P302913   

Export aliases for WEE1 gene to outside databases

Previous GC identifers: GC11P010605 GC11P010164 GC11P009633 GC11P009558 GC11P009550 GC11P009266


Summaries
for WEE1 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for WEE1:
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases.
This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the
transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
(provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: WEE1_HUMAN, P30291
Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from
cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on
'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and
a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation
of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is
hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation

Gene Wiki entry for WEE1 (Wee1-like protein kinase)


Genomic Views
for WEE1 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000011.9  NC_018922.1  NT_009237.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the WEE1 gene promoter:
         p53   MyoD   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 4): WEE1 promoter sequence
   Search SABiosciences Chromatin IP Primers for WEE1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WEE1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p15.3-p15.1   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15.3-p15.1

WEE1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WEE1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P009595:  view genomic region     (about GC identifiers)

Start:
 9,595,228 bp from pter      End:
 9,615,004 bp from pter
Size:
 19,777 bases      Orientation:
 plus strand

Proteins
for WEE1 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: WEE1_HUMAN, P30291 (See protein sequence)
Recommended Name: Wee1-like protein kinase  
Size: 646 amino acids; 71597 Da
Cofactor: Binds 2 magnesium ions per subunit
Subcellular location: Nucleus
6/8 PDB 3D structures from and Proteopedia for WEE1 (see all 8):
1X8B (3D)        2IN6 (3D)        2IO6 (3D)        2Z2W (3D)        3BI6 (3D)        3BIZ (3D)    
Secondary accessions: B3KVE1 D3DQV0
Alternative splicing: 2 isoforms:  P30291-1   P30291-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for WEE1: NX_P30291

Post-translational modifications:

  • Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in
    • post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123
    by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex,
    leading to its ubiquitination and degradation at the onset of G2/M phase1
  • Dephosphorylated at Thr-239 by CTDP11
  • Ubiquitinated and degraded at the onset of G2/M phase1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P30291

    • 4/5 DME Specific Peptides for WEE1 (P30291) (see all 5)
     WAEDDHM  RLDGCIYAIK  VEEGDSRFLANE  YAIKRSKKPLAGS 

    WEE1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001137448.1  NP_003381.1  

    ENSEMBL proteins: 
     ENSP00000402084   ENSP00000299613   ENSP00000434446   ENSP00000434148   ENSP00000435945  
     ENSP00000432284  
    Reactome Protein details: P30291
    Human Recombinant Protein Products: 
    EMD Millipore Purified and/or Recombinant WEE1 Protein
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein: WEE1
    OriGene Protein Over-expression Lysate: WEE1
    OriGene Custom Protein Services for WEE1 
    GenScript Custom Purified and Recombinant Proteins Services for WEE1
    Novus Biologicals WEE1 Proteins
    Novus Biologicals WEE1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for WEE1

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus TAS8348613
    GO:0005654nucleoplasm TAS--
    GO:0005737cytoplasm IEA--


    WEE1 for ontologies           About GeneDecksing



    WEE1 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of WEE1
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    Cell Signaling Technology (CST) Antibodies for WEE1 
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    Novus Biologicals WEE1 Antibodies
    Abcam antibodies for WEE1 
    Uscn Antibodies for WEE1
    ThermoFisher Antibodies for WEE1

    Assay Products for WEE1: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for WEE1
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for WEE1

    Protein Domains /
    Families
    for WEE1 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    WEE1 for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR017164 Wee1-like_protein_kinase
     IPR017441 Protein_kinase_ATP_BS
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_cat_dom

    Graphical View of Domain Structure for InterPro Entry P30291

    ProtoNet protein and cluster: P30291

    UniProtKB/Swiss-Prot: WEE1_HUMAN, P30291
    Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily
    Similarity: Contains 1 protein kinase domain


    Function
    for WEE1 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: WEE1_HUMAN, P30291
    Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from
    cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on
    'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and
    a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation
    of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is
    hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation
    Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
    Enzyme regulation: Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity
    is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis
    combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis,
    although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might
    regulate the subcellular location

         Genatlas biochemistry entry for WEE1:
    wee1 tyrosine kinase,yeast wee1 homolog,involved in cell cycle regulation

    Enzyme Number (IUBMB): EC 2.7.10.21 2

    miRNA
    Products:            		miRTarBase miRNAs that target WEE1:
    hsa-mir-17 (MIRT005853), hsa-mir-195 (MIRT000222), hsa-mir-106b (MIRT005868), hsa-mir-424 (MIRT000659), hsa-mir-20a (MIRT005860), hsa-mir-503 (MIRT000644), hsa-mir-372 (MIRT000057)

    OriGene 3'-UTR Clone (see all 2): WEE1
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat WEE1
    8/45 QIAGEN miScript miRNA Assays for microRNAs that regulate WEE1 (see all 45):
    hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-106a hsa-miR-15a hsa-miR-503 hsa-miR-219-5p hsa-miR-508-3p
    SwitchGear 3'UTR luciferase reporter plasmid: WEE1 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WEE1 (see all 7)
    OriGene shRNA RFP: WEE1
    OriGene siRNA: WEE1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WEE1

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for WEE1

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WEE1 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WEE1 (see all 3)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 2): WEE1 (NM_003390)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WEE1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WEE1 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for WEE1
    Search LifeMap BioReagents cell lines for WEE1

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WEE1

    Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287magnesium ion binding IEA--
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IEA--
    GO:0004713protein tyrosine kinase activity TAS7774574
    GO:0004715non-membrane spanning protein tyrosine kinase activity IEA--


    WEE1 for ontologies           About GeneDecksing


    9 GenomeRNAi human phenotypes for WEE1:
     Cell cycle / mitosis defect  Decreased cell number, increas  Decreased substrate adherent c  Gemcitabine induced cell-death 
     Increased cell death in HCC-19  Increased cell number in S  Increased number of mitotic ce  S arrest 
     Upregulation of Wnt/beta-caten 

    Animal Models:
         1 MGI phenotypic allele for Wee1 (no phenotypes)

    WEE1 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for WEE1 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/20 super-pathways (see all 20) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Ubiquitinated Orc1 is degraded by the proteasome
    		Cyclin E associated events during G1/S transition 0.67
    		Cell Cycle Checkpoints0.42
    		Cyclin A:Cdk2-associated events at S phase entry0.66
    		S Phase0.40
    2G2/M DNA damage checkpoint
    		G2/M DNA damage checkpoint1.00
    		G2/M DNA replication checkpoint0.30
    		Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex0.56
    3Cell cycle
    		Cell cycle1.00
    		G1 to S cell cycle control0.38
    		Cell cycle0.57
    4Cell cycle_Role of 14-3-3 proteins in cell cycle regulation
    		Cell cycle_Role of 14-3-3 proteins in cell cycle regulation1.00
    		14-3-3 and Cell Cycle Regulation0.45
    		Cell cycle Role of 14-3-3 proteins in cell cycle regulation1.00
    5Cell cycle_Role of SCF complex in cell cycle regulation
    		Cell cycle_Role of SCF complex in cell cycle regulation1.00
    		Cell cycle Role of SCF complex in cell cycle regulation0.98

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    2 EMD Millipore Pathways for WEE1
        Cell cycle Role of 14-3-3 proteins in cell cycle regulation
    Cell cycle Role of SCF complex in cell cycle regulation

    4 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for WEE1
        14-3-3 and Cell Cycle Regulation
    Cyclins and Cell Cycle Regulation
    Molecular Mechanisms of Cancer
    Mitotic Roles of Polo Like Kinases

    2 Cell Signaling Technology (CST) Pathways for WEE1
        Cell Cycle / Checkpoint Control
    DNA Damage

    2 GeneGo (Thomson Reuters) Pathways for WEE1
        Cell cycle Role of SCF complex in cell cycle regulation
    Cell cycle Role of 14-3-3 proteins in cell cycle regulation

    4 BioSystems Pathways for WEE1 
        Cell cycle
    Integrated Breast Cancer Pathway
    G1 to S cell cycle control
    PLK1 signaling events

    5/18        Reactome Pathways for WEE1 (see all 18)
        Mitotic G1-G1/S phases
    Cell Cycle
    S Phase
    Factors involved in megakaryocyte development and platelet production
    G2/M Transition


    1         Kegg Pathway  (Kegg details for WEE1):
        Cell cycle


    WEE1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for WEE1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/51 Interacting proteins for WEE1 (P302911, 2, 3 ENSP000004020844) via UniProtKB, MINT, STRING, and/or I2D (see all 51)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    YWHABP319462, 3, ENSP000003001614MINT-16603 MINT-16602 MINT-50988 I2D: score=4 STRING: ENSP00000300161
    YWHAZP631042, 3MINT-3294834 MINT-3294458 MINT-3295375 I2D: score=2 
    BTRCQ9Y2971, 3, ENSP000003592064EBI-914695,EBI-307461 I2D: score=4 STRING: ENSP00000359206
    SFNP319472, 3, ENSP000003409894MINT-3975741 I2D: score=2 STRING: ENSP00000340989
    FBXW11Q9UKB11, 3, ENSP000002650944EBI-914695,EBI-355189 I2D: score=4 STRING: ENSP00000265094
    About this table

    Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000075cell cycle checkpoint TAS--
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000084S phase of mitotic cell cycle TAS--
    GO:0000086G2/M transition of mitotic cell cycle TAS--
    GO:0000226microtubule cytoskeleton organization IEA--


    WEE1 for ontologies           About GeneDecksing



    Drugs & Compounds
    for WEE1 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    WEE1 for compounds           About GeneDecksing

    EMD Millipore small molecules for WEE1:
    Small Molecule - inhibitor
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for WEE1

    3 HMDB Compounds for WEE1    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--

    6 DrugBank Compounds for WEE1    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    3-(9-HYDROXY-1,3-DIOXO-4-PHENYL-2,3-DIHYDROPYRROLO[3,4-C]CARBAZOL-6(1H)-YL)PROPANOIC ACID-- --target--10592235
    4-(2-chlorophenyl)-8-(2-hydroxyethyl)-6-methylpyrrolo[3,4-e]indole-1,3(2H,6H)-dione-- --target--10592235
    8-bromo-4-(2-chlorophenyl)-N-(2-hydroxyethyl)-6-methyl-1,3-dioxo-1,2,3,6-tetrahydropyrrolo[3,4-e]indole-7-carboxamide-- --target--10592235
    9-HYDROXY-4-PHENYL-6H-PYRROLO[3,4-C]CARBAZOLE-1,3-DIONE-- --target--10592235
    9-HYDROXY-6-(3-HYDROXYPROPYL)-4-(2-METHOXYPHENYL)PYRROLO[3,4-C]CARBAZOLE-1,3(2H,6H)-DIONE-- --target--10592235
    N-[4-(2-CHLOROPHENYL)-1,3-DIOXO-1,2,3,6-TETRAHYDROPYRROLO[3,4-C]CARBAZOL-9-YL]FORMAMIDE-- --target--10592235

    4 Novoseek chemical compound relationships for WEE1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 53.8 24 14711880 (2), 7813446 (2), 1384126 (1), 15558017 (1) (see all 19)
    berberine 36.1 5 16619512 (4), 16440412 (1)
    curcumin 31 3 17201156 (1), 14534529 (1), 16596191 (1)
    oxygen 0 1 17201156 (1)

    Search CenterWatch for drugs/clinical trials and news about WEE1 

    Transcripts
    for WEE1 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for WEE1 gene (2 alternative transcripts): 
    NM_001143976.1  NM_003390.3  

    Unigene Cluster for WEE1:

    WEE1 homolog (S. pombe)
    Hs.249441  [show with all ESTs]
    Unigene Representative Sequence: BC070052
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000450114(uc001mhs.3) ENST00000299613(uc001mht.3) ENST00000524549(uc001mhu.3)
    ENST00000524612 ENST00000530712 ENST00000530175 ENST00000532275 ENST00000527848


    miRNA
    Products:             		 OriGene 3'-UTR Clone (see all 2): WEE1
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat WEE1
    8/45 QIAGEN miScript miRNA Assays for microRNAs that regulate WEE1 (see all 45):
    hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-106a hsa-miR-15a hsa-miR-503 hsa-miR-219-5p hsa-miR-508-3p
    SwitchGear 3'UTR luciferase reporter plasmid: WEE1 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WEE1 (see all 7)
    OriGene shRNA RFP: WEE1
    OriGene siRNA: WEE1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WEE1
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WEE1 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WEE1 (see all 3)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 2): WEE1 (NM_003390)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WEE1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WEE1 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WEE1
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat WEE1
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WEE1
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WEE1

    Additional cDNA sequence: 

    AK122837.1 BC051831.1 BC070052.1 U10564.1 X62048.1 

    8 DOTS entries:

    DT.315661  DT.95290127  DT.92432867  DT.95373536  DT.100036218  DT.100787721  DT.120680022  DT.120680029 

    24/226 AceView cDNA sequences (see all 226):

    BM718727 T28628 BG611243 U10564 BQ024511 CB113055 AI589239 BC070052 
    BM695000 BU689760 AU126299 CB149989 BF478052 BE349189 AI473778 N34248 
    BX117974 AW949527 BU619270 AI474012 AI961592 AU280199 BC051831 CD517624 

    GeneLoc Exon Structure


    Expression
    for WEE1 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    WEE1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTTGCACTTG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    WEE1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 2 LifeMap Cells 
    NameCategory
    PureStem™ progenitor U31 (Embryonic Progenitor Cell)
    Line H7 (WA07) (Embryonic Stem Cell)Early Embryo, Inner Cell Mass
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See WEE1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for WEE1

    SOURCE GeneReport for Unigene cluster: Hs.249441
        SABiosciences Expression via Pathway-Focused PCR Arrays including WEE1: 
              Cell Cycle in human mouse rat
              Circadian Rhythms in human mouse rat
              Cancer PathwayFinder in human mouse rat

    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WEE1
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    In Situ
    Assay Products:     
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    Orthologs
    for WEE1 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for WEE1 gene from 7/24 species (see all 24)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves WEE11 WEE1 homolog (S. pombe) 77.19(n)
    81.5(a)    		   423045  NM_001031181.2  NP_001026352.2 
    lizard
    (Anolis carolinensis)    		 Reptilia WEE16
    		 --
    		 75(a)
    		 1 ↔ 1
    		 1(64144482-64158740)
    African clawed frog
    (Xenopus laevis)    		 Amphibia Xl.173932 Xenopus laevis mRNA for Wee1B kinase, complete cds 80.09(n)    AB071983.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii Dr.283462 Danio rerio cDNA clone IMAGE3815602, partial cds 77.65(n)    BC058071.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta wee1 , 3 mitotic checkpoint protein tyrosine kinase3
    CG4488-PA1    		 43(a)3
    50.87(n)1
    48.75(a)1    		   2 27C43
    339651  NM_057687.31  NP_477035.11 
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons WEE16
    		 Wee1-like protein kinase
    		 22(a)
    		 1 → many
    		 1(673306-676378)
    rice
    (Oryza sativa)    		 Liliopsida --
    		 wee1-like protein kinase, putative, expressed
    		 23(a)
    		 1 → many
    		 2(1859994-1864612)


    ENSEMBL Gene Tree for WEE1 (if available)
    TreeFam Gene Tree for WEE1 (if available) 

    Paralogs
    for WEE1 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for WEE1 gene
    WEE22  PKMYT12  
    3 SIMAP similar genes for WEE1 using alignment to 7 protein entries:     WEE1_HUMAN (see all proteins):
    DKFZp686I18166    WEE2    PKMYT1

    WEE1 for paralogs           About GeneDecksing


    5 Pseudogenes.org Pseudogenes for WEE1
    PGOHUM00000236992 PGOHUM00000237324 PGOHUM00000245419 PGOHUM00000245420 PGOHUM00000245423


    Genomic Variants
    for WEE1 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/285 NCBI SNPs in WEE1 are shown (see all 285)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 11 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs37638721,2
    		C,F,A,H,--9593427(-) ACATCG/AGTGGA 1 -- us2k124Minor allele frequency- A:0.32EA NS NA WA CSA 3839
    rs79406721,2
    		C,F,--9593574(+) GGCCAG/AGCGCG 1 -- us2k16Minor allele frequency- A:0.16NA WA EA 364
    rs1901861011,2
    		--9593608(+) CACTTC/TGGGAG 1 -- us2k10--------
    rs1453731771,2
    		--9593627(+) GGGCAC/GATCAC 1 -- us2k10--------
    rs1826376821,2
    		--9593668(+) AACATA/GGTGAA 1 -- us2k10--------
    rs1873177221,2
    		--9593781(+) AACCCA/GGGAGG 1 -- us2k10--------
    rs114441711,2
    		C,--9593857(+) TGTCGA/-AAAAA 1 -- us2k11Minor allele frequency- -:0.00CSA 2
    rs113703751,2
    		C--9593858(+) AAAAA-/ACCAAA 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs754099041,2
    		C,--9593870(+) CCAAAC/GCAAAC 1 -- us2k13Minor allele frequency- G:0.06NA WA 240
    rs618768301,2
    		C,--9594137(+) CGCTTC/GAACTC 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for WEE1 (9595228 - 9615004 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for WEE1: --

    SABiosciences Cancer Mutation PCR Assays
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    Disorders / Diseases
    for WEE1 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    WEE1 for disorders           About GeneDecksing

    OMIM gene information: 193525    OMIM disorders: --

    20/27 diseases for WEE1 (see all 27):    About MalaCards
    beckwith-wiedemann syndrome    suppurative lymphadenitis    oral squamous cell carcinoma    acute promyelocytic leukemia
    squamous cell carcinoma    lymphadenitis    sporadic breast cancer    chronic lymphocytic leukemia
    lymphocytic leukemia    pituitary adenoma    insulin resistance    lipodystrophy
    breast cancer    leukemia    adenoma    hepatocellular carcinoma
    neurodegeneration    infertility    nasopharyngitis    immunodeficiency

    1 disease from the University of Copenhagen DISEASES database for WEE1:
    Suppurative lymphadenitis

    6 Novoseek disease relationships for WEE1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    beckwith-wiedemann syndrome 40.1 1 7842740 (1)
    cancer 18.8 7 19500427 (2), 20159553 (1), 11585773 (1), 15735666 (1)
    tumors 3.68 7 14760118 (1), 19288185 (1), 19887545 (1), 14726685 (1) (see all 7)
    leukemia 0 2 16619512 (1), 18351645 (1)
    cancer lung 0 2 14760118 (2)
    immunodeficiency 0 1 16968670 (1)

    Human Genome Epidemiology (HuGE) Navigator: WEE1 (5 documents)
    Tumor Gene Database (TGDB): WEE1

    Export disorders for WEE1 gene to outside databases

    Publications
    for WEE1 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for WEE1 gene, integrated from 9 sources (see all 215):
    (articles sorted by number of sources associating them with WEE1)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Wee1(+)-like gene in human cells. (PubMed id 1840647)1, 2, 3 Igarashi M.... Okayama H. (1991)
    2. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. (PubMed id 7743995)1, 2, 9 Watanabe N.... Hunter T. (1995)
    3. Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15. (PubMed id 8428596)1, 2, 9 McGowan C.H. and Russell P. (1993)
    4. Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1). (PubMed id 11528126)1, 2, 9 Cichutek A.... Zabel B.U. (2001)
    5. M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP. (PubMed id 15070733)1, 2, 9 Watanabe N....Osada H. (2004)
    6. Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. (PubMed id 15837193)1, 2, 9 Squire C.J.... Baker E.N. (2005)
    7. Fcp1-dependent dephosphorylation is required for M-phase-promoting factor inactivation at mitosis exit. (PubMed id 22692537)1, 2 Visconti R.... Grieco D. (2012)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    9. Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. (PubMed id 15150265)1, 2 Lu R.... Nakanishi M. (2004)
    10. A bifunctional regulatory element in human somatic We e1 mediates cyclin A/Cdk2 binding and Crm1-dependent nuclear export. (PubMed id 19858290)1, 9 Li C....Enders G.H. (2010)

    External Searches for WEE1 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing WEE1 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 7465 HGNC: 12761 AceView: WEE1 Ensembl:ENSG00000166483 euGenes: HUgn7465
    ECgene: WEE1 Kegg: 7465 H-InvDB: WEE1

    Other Databases showing WEE1 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing WEE1 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for WEE1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for WEE1 Genetics and Cytogenetics in Oncology and Haematology

    Intellectual Property
    for WEE1 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for WEE1 gene:
    Search GeneIP for patents involving WEE1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
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    About This Section

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