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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

WDYHV1 Gene

protein-coding   GIFtS: 45
GCID: GC08P124428

WDYHV motif containing 1

(Previous name: chromosome 8 open reading frame 32 )
(Previous symbol: C8orf32)
 Explore 1 disease affiliated with
WDYHV1 via our new
 Human Malady Compendium 
Biological research products
for WDYHV1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
WDYHV Motif Containing 11 2     Chromosome 8 Open Reading Frame 321
C8orf321 2 3     Nt(Q)-Amidase1
Protein NH2-Terminal Glutamine Deamidase2 3     Protein N-Terminal Glutamine Amidohydrolase2
N-Terminal Gln Amidase2 3     EC 3.5.1.-3
WDYHV Motif-Containing Protein 12 3     NTAQ13
FLJ102041     Nt(Q)-Amidase1

External Ids:    HGNC: 254901   Entrez Gene: 550932   Ensembl: ENSG000001567957   UniProtKB: Q96HA83   

Export aliases for WDYHV1 gene to outside databases

Previous GC identifers: GC08P124500 GC08P119754


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: NTAQ1_HUMAN, Q96HA8
Function: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end
rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein
susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on
substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not
deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on
substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes
deamidation of N-terminal glutamine (By similarity)

Gene Wiki entry for WDYHV1 (C8orf32)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000008.10  NC_018919.1  NT_008046.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the WDYHV1 gene promoter:
         HFH-3   AML1a   RREB-1   FOXI1   CREB   HEN1   deltaCREB   FOXO4   ZIC2/Zic2   TGIF   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for WDYHV1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WDYHV1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24.13   Ensembl cytogenetic band:  8q24.13   HGNC cytogenetic band: 8q24.13

WDYHV1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WDYHV1 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P124428:  view genomic region     (about GC identifiers)

Start:
124,428,965 bp from pter      End:
124,479,470 bp from pter
Size:
50,506 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NTAQ1_HUMAN, Q96HA8 (See protein sequence)
Recommended Name: Protein N-terminal glutamine amidohydrolase  
Size: 205 amino acids; 23680 Da
Subunit: Monomer
Subcellular location: Cytoplasm, cytosol (By similarity). Nucleus (By similarity)
1 PDB 3D structure from and Proteopedia for WDYHV1:
3C9Q (3D)    
Secondary accessions: Q9NW95

Explore the universe of human proteins at neXtProt for WDYHV1: NX_Q96HA8

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96HA8

  • WDYHV1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_060494.1  
    ENSEMBL proteins: 
     ENSP00000287387   ENSP00000430427   ENSP00000434252   ENSP00000430390   ENSP00000428615  
     ENSP00000429258  

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    Uscn Proteins for WDYHV1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ISS--
    GO:0005829cytosol ISS--


    WDYHV1 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for WDYHV1


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    WDYHV1 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR023128 Prot_N_Gln_amidohydro_ab_roll

    Graphical View of Domain Structure for InterPro Entry Q96HA8

    ProtoNet protein and cluster: Q96HA8

    UniProtKB/Swiss-Prot: NTAQ1_HUMAN, Q96HA8
    Similarity: Belongs to the NTAQ1 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: NTAQ1_HUMAN, Q96HA8
    Function: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end
    rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein
    susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on
    substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not
    deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on
    substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes
    deamidation of N-terminal glutamine (By similarity)

    Enzyme Number (IUBMB): EC 3.5.1.-1

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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI19060904
    GO:0016811hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides ----
    GO:0070773protein-N-terminal glutamine amidohydrolase activity ISS--


    WDYHV1 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for WDYHV1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 24)

    5/33 Interacting proteins for WDYHV1 (Q96HA81, 2, 3 ENSP000002873874) via UniProtKB, MINT, STRING, and/or I2D (see all 33)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HPRT1P004921, 2, 3, ENSP000002985564EBI-741158,EBI-748210 MINT-67224 I2D: score=5 STRING: ENSP00000298556
    HSD17B14Q9BPX11, 2, 3, ENSP000002632784EBI-741158,EBI-742664 MINT-66311 I2D: score=5 STRING: ENSP00000263278
    JUPP149231, 2, 3, ENSP000003111134EBI-741158,EBI-744449 MINT-66801 I2D: score=5 STRING: ENSP00000311113
    NME1P155311, 2, 3, ENSP000000130344EBI-741158,EBI-741141 MINT-66144 I2D: score=5 STRING: ENSP00000013034
    SMN1Q166372, 3, ENSP000003700834MINT-68375 I2D: score=5 STRING: ENSP00000370083
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006464cellular protein modification process ISS--


    WDYHV1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for WDYHV1
    Search CenterWatch for drugs/clinical trials and news about WDYHV1 / NTAQ1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for WDYHV1 gene: 
    NM_018024.1  

    Unigene Cluster for WDYHV1:

    WDYHV motif containing 1
    Hs.18029  [show with all ESTs]
    Unigene Representative Sequence: AK293492
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000287387(uc003yqn.1) ENST00000523984(uc011lij.1) ENST00000524254
    ENST00000522194 ENST00000517609 ENST00000519199 ENST00000523551 ENST00000523356
    ENST00000518125

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    Additional cDNA sequence: 

    AK001066.1 AK225643.1 AK293492.1 BC008781.2 

    6 DOTS entries:

    DT.449017  DT.100788165  DT.91763765  DT.91763760  DT.100788162  DT.95088716 

    24/136 AceView cDNA sequences (see all 136):

    AI093302 CB161316 AI871929 AL538334 BU632739 BM468411 BF437211 AI400386 
    AI057379 CA313379 BE673522 AK001066 BI916514 NM_018024 AI399793 AU119047 
    AI271660 CR600640 AI422883 BX119233 AA253324 BF973681 BE889754 CB104761 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for WDYHV1    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d
    SP1:                    -                                                               
    SP2:                                            -                                       
    SP3:                    -                       -                                       
    SP4:                                                                                    
    SP5:                                            -     -                                 


    ECgene alternative splicing isoforms for WDYHV1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    WDYHV1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See WDYHV1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for WDYHV1

    SOURCE GeneReport for Unigene cluster: Hs.18029
        SABiosciences Custom PCR Arrays for WDYHV1
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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for WDYHV1 gene from 9/24 species (see all 24)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Wdyhv11 , 5 WDYHV motif containing 11, 5 83.09(n)1
    85.22(a)1
      15 (24.38 cM)5
    767731  NM_029734.11  NP_084010.11 
     581414365 
    chicken
    (Gallus gallus)
    Aves WDYHV11 WDYHV motif containing 1 74.69(n)
    70.68(a)
      420345  XM_418452.3  XP_418452.1 
    lizard
    (Anolis carolinensis)
    Reptilia WDYHV16
    --
    65(a)
    1 ↔ 1
    4(6784763-6792124)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.127942 Transcribed sequence with weak similarity to protein more 74.67(n)    BX716305.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wdyhv11 WDYHV motif containing 1 67.51(n)
    65.99(a)
      560935  NM_001044947.1  NP_001038412.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta tun1 tungus 50.09(n)
    49.19(a)
      36743  NM_137217.2  NP_611061.1 
    worm
    (Caenorhabditis elegans)
    Secernentea R06C7.61 Protein R06C7.6 49.79(n)
    41.25(a)
      187641  NM_059647.1  NP_492048.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT2G417601 protein N-terminal glutamine amidohydrolase 54.62(n)
    39.35(a)
      818775  NM_129740.3  NP_565959.1 
    rice
    (Oryza sativa)
    Liliopsida Os05g03879001 hypothetical protein 54.64(n)
    48.52(a)
      4338665  NM_001061962.1  NP_001055427.1 


    ENSEMBL Gene Tree for WDYHV1 (if available)
    TreeFam Gene Tree for WDYHV1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/646 NCBI SNPs in WDYHV1 are shown (see all 646    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1874234201,2
    --124426971(+) CTGGTC/TTTTGT 1 -- us2k10--------
    rs1904547181,2
    --124427015(+) AAACCC/TGCAAG 1 -- us2k10--------
    rs1396169901,2
    --124427302(+) CAAGAC/TTTAGA 1 -- us2k10--------
    rs1495729861,2
    --124427403(+) TGATGC/TTTAGT 1 -- us2k10--------
    rs1442389301,2
    --124427605(+) ACACAC/GGAACT 1 -- us2k10--------
    rs1816681301,2
    --124427811(+) TATTAA/GTATCA 1 -- us2k10--------
    rs113065611,2
    C,F--124427841(+) CACTA-/TTTTTT 1 -- us2k1 trp33Minor allele frequency- T:0.33NA CSA 6
    rs1487099291,2
    --124427978(+) TACAGA/GCGCGC 1 -- us2k10--------
    rs1855577381,2
    --124428024(+) GAGACA/GGAGTT 1 -- us2k10--------
    rs1424660251,2
    --124428115(+) GGCGTA/GAGCCA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for WDYHV1 (124428965 - 124479470 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for WDYHV1: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    WDYHV1 for disorders           About GeneDecksing

    1 disease for WDYHV1:    About MalaCards
    ataxia


    Export disorders for WDYHV1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for WDYHV1 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with WDYHV1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (2002)
    3. An empirical framework for binary interactome mapping . (PubMed id 19060904)1 Venkatesan K....Vidal M. (2009)
    4. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
    5. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. (PubMed id 16713569)1 Lim J.... Zoghbi H.Y. (2006)
    6. Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (2005)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    8. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)1 Maruyama K. and Sugano S. (1994)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 55093 HGNC: 25490 AceView: FLJ10204 Ensembl:ENSG00000156795 euGenes: HUgn55093
    ECgene: WDYHV1 H-InvDB: WDYHV1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for WDYHV1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for WDYHV1 gene:
    Search GeneIP for patents involving WDYHV1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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