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WDR33 Gene

protein-coding   GIFtS: 53

GC02M128179
WD repeat domain 33
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ11294 1, 2
WDC146 1, 2, 3
Descriptions
WD repeat domain 33 2
WD repeat-containing protein WDC146 3
External Ids
HGNC: 256511
Entrez Gene: 553392
UniProtKB: Q9C0J83
Ensembl: ENSG000001367097
Search outside databases for aliases for WDR33 gene

Previous GC identifer: GC02M128557

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for WDR33:
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved
regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which
may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are
involved in a variety of cellular processes, including cell cycle progression, signal
transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the
protein is localized to the nucleus. This gene may play important roles in the mechanisms of
cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants
encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Gene Wiki entry for WDR33
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WDR33 gene  

Entrez Gene cytogenetic band: 2q14.3   Ensembl cytogenetic band:  2q14.3   HGNC cytogenetic band: 2q21.1

WDR33 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M128179:     (about GC identifiers)

Start:
128,178,278 bp from pter
End:
128,285,215 bp from pter
Size:
106,938 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_022135.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: WDR33_HUMAN, Q9C0J8 (See protein sequence)
Recommended Name: WD repeat-containing protein 33  
Size: 1336 amino acids; 145891 Da
Subcellular location: Nucleus
Secondary accessions: Q587J1

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_001006623.1  NP_001006624.1  NP_060853.3  


    ENSEMBL proteins: 
    ENSP00000325377 ENSP00000376730 ENSP00000387186 ENSP00000386861 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (WDR33)
    Human Recombinant Proteins from Abnova (WDR33)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA11162572
    About this table

    Antibodies for WDR33: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for WDR33
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (WDR33), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibodies for WDR33

    Assays for WDR33: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/7 InterPro domains/families (see all 7 ):
     IPR019782 WD40_repeat_2
     IPR019775 WD40_repeat_CS
     IPR019781 WD40_repeat_sg
     IPR001680 WD40_repeat
     IPR017986 WD40_repeat_region


       GeneDecks  WDR33 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9C0J8

    ProtoNet protein and cluster: Q9C0J8

    1 Blocks protein family: IPB008160 Collagen triple helix repeat

    UniProtKB/Swiss-Prot: WDR33_HUMAN, Q9C0J8
    Similarity: Contains 1 collagen-like domain
    Similarity: Contains 7 WD repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDR33)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001006622

                  Applied Biosystems Silencer® siRNAs for WDR33

                  Sigma-Aldrich siRNA and siRNA Panels for WDR33  
                         Sigma-Aldrich shRNA for WDR33  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for WDR33
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001006622
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001006622
                                     untagged cDNA clones in CMV expression vector (see all 4): BC013990 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018383

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674 molecular_function ND--
    GO:0005515 protein binding IPI17353931
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for WDR33 (Q9C0J81) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RNPS1Q152871EBI-712637, EBI-395959
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006301 postreplication repair NAS11162572
    GO:0007283 spermatogenesis NAS11162572
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for WDR33

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDR33)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001006622

                  Sigma-Aldrich siRNA and siRNA Panels for WDR33  
                         Sigma-Aldrich shRNA for WDR33  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001006622  NM_001006623  NM_018383  

    REFSEQ mRNAs for WDR33 gene (3 alternative transcripts): 

    NM_001006622.1   NM_001006623.1   NM_018383.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001006622  NM_001006623  NM_018383  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001006622
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001006622
                                     untagged cDNA clones in CMV expression vector (see all 4): BC013990 

    Additional cDNA sequence: 

    AB044749.1 AK002156.1 AK098003.1 AK123510.1 AK223319.1 AL834365.1 BC005401.1 BC010283.1 
    BC013990.2 BC068484.1 BC108671.1 CR591938.1 

    20 DOTS entries:

    DT.85105571  DT.101981406  DT.210433  DT.99988925  DT.410657  DT.95193952  DT.114076  DT.95277296 
    DT.425610  DT.100777349  DT.216281  DT.100751287  DT.100777350  DT.40121162  DT.92436263  DT.95287644 
    DT.120966393  DT.120966449  DT.121488446  DT.91988481 

    24/298 AceView cDNA sequences (see all 298 ):

    AA773744 CK820474 BC068484 BX103711 AI039494 BG283913 BE859089 BM751741 
    BQ277834 AA491257 BQ896760 BF063402 CA395595 Z41772 BX115033 F10034 
    AA284061 BF931513 BM928629 BQ011321 AA927896 BM718079 BU538276 AA339047 

    highest scoring ESTs for WDR33:

    AA827926 AI480300 N48582 AA460084 AA630806 AB044749 AK002156 AL561047 AU138991 BC005401 

    Unigene Cluster for WDR33:

    WD repeat domain 33
    Hs.620490  [show with all ESTs]
    Unigene Representative Sequence: NM_018383


    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for WDR33 (see all 8 )

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^
    SP1:                    -     -                                         -           -     -     -                             -                                 
    SP2:                    -     -                                         -           -     -                                                                     
    SP3:                                                                                                                                                            
    SP4:                    -     -                                                                                                                                 
    SP5:                    -                                                                                                                                       

    ExUns: 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25 ^ 26 ^ 27
    SP1:                                                            
    SP2:                                                            
    SP3:                    -     -                                 
    SP4:                                                            
    SP5:                                                            

    About this scheme

    ECgene alternative splicing isoforms for WDR33

    4 Ensembl transcripts including schematic representations:
    ENST00000322313  ENST00000393006  ENST00000409658  ENST00000408998  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    WDR33 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for WDR33

    1 / 2 / 3

    16 probe-sets matching WDR33 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    48067_at2, 3 U95-B 1 1.00 1.00 0.62 1.21 R24833 0.20 1.00 0.72 1

    62171_at2, 3 U95-C 1 1.00 1.00 0.75 1.32 AA827926 0.80 1.00 0.91 1

    76384_at2, 3 U95-D 1 1.00 1.00 0.80 1.09 AI791123 0.40 1.00 0.76 1

    82067_at2, 3 U95-E 1 1.00 1.00 0.80 0.50 AI480300 0.80 1.00 0.91 1
    47485_at2, 3 U95-B 1 1.00 1.00 0.85 1.01 AA259167 0.60 1.00 0.82 1
    53512_at2, 3 U95-B 2 0.94 0.94 0.71 0.91 N48582 0.80 1.00 0.91 1

    223147_s_at2, 3 U133-B 1 1.00 1.00 -- -- AB044749 0.80 0.88 0.84 1

    222763_s_at2, 3 U133-B 1 1.00 1.00 -- -- AW161711 0.60 1.00 0.82 1

    229141_at2, 3 U133-B 1 1.00 1.00 -- -- AW275379 0.40 1.00 0.76 1

    218851_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018383 0.60 1.00 0.82 1

    223146_at2, 3 U133-B 1 1.00 1.00 -- -- BE222527 0.40 1.00 0.76 1

    223147_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222763_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    229141_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    218851_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    223146_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  WDR33 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGCTCTGTCA

    SOURCE GeneReport for Unigene cluster: Hs.620490

    UniProtKB/Swiss-Prot: WDR33_HUMAN, Q9C0J8
    Tissue specificity: Most highly expressed in testis

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for WDR33 gene from 5/19 species (see all 19 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    WDR331   -- WD repeat domain 33 94.24(n)
    98.43(a)
    483865  XM_540985.2  XP_540985.2 
    chimpanzee
    (Pan troglodytes)
    WDR331   -- WD repeat domain 33 99.83(n)
    99.93(a)
    470527  XM_001139347.1  XP_001139347.1 
    rat
    (Rattus norvegicus)
    Wdr331   -- WD repeat domain 33 90.43(n)
    97.07(a)
    307524  XM_226076.4  XP_226076.2 
    mouse
    (Mus musculus)
    Wdr331, 5 185
    WD repeat domain 331, 5 90.24(n)1
    96.54(a)1
    743201  NM_028866.21  NP_083142.21 
     AA3872035  AB0861915  (see all 28)
    chicken
    (Gallus gallus)
    WDR331   -- WD repeat domain 33 81.33(n)
    90.51(a)
    424753  XM_422575.2  XP_422575.2 
    About this table        Species with no ortholog for WDR33

    ENSEMBL Gene Tree for WDR33
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for WDR33 gene
    PRB42  PRB32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/648 NCBI SNPs in WDR33 are shown (see all 648 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 177)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs75813601,2
    A,F,H128285718(+) CCacgA/Gtgcaa 3 -- ng314Minor allele frequency- G:0.19EU EA WA 408
    rs175997711,2
    C,F,H128208989(+) TGCCTA/TATGTG 2 -- int1 ng517Minor allele frequency- T:0.09NA EU EA WA 546
    rs101864041,2
    A,F,H128285495(+) GGGCCA/GGTACG 3 -- ng314Minor allele frequency- G:0.02EU EA WA 418
    rs175341231,2
    C,F,H128238677(+) GATACC/TGGGAC 3 R/Q mis1 int116Minor allele frequency- T:0.05NA EU EA WA 1474
    rs29231,2
    C,F,H128180088(+) CATGTC/GTTGTG 1 -- ut31 ese38Minor allele frequency- G:0.02MN NA EU EA WA 742
    rs101889861,2
    A,F,H128285437(+) ACGTGT/GATGCT 3 -- ng314Minor allele frequency- G:0.02EU EA WA 418
    rs134080781,2
    A,F,H128210693(+) GTCAAA/GTAGGT 2 -- ut31 int14Minor allele frequency- G:0.02EU EA WA 418
    rs75671851,2
    F,H128285313(+) GGTGCC/AGAGCC 3 -- ng314Minor allele frequency- A:0.01EU EA WA 408
    rs116866071,2
    C,H128211735(+) GAACCA/GAGAAG 2 -- ut31 int1 trp34Minor allele frequency- G:0.00EU EA WA 414
    rs126150781,2
    F,H128193937(+) GGCCAG/CGTGGA 1 R/P mis1 ese34Minor allele frequency- C:0.01EU EA WA 394
    About this table

    HapMap Linkage Disequilibrium images for WDR33 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for WDR33 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55339 HGNC: 25651 AceView: WDR33 Ensembl:ENSG00000136709 euGenes: HUgn55339
    ECgene: WDR33 H-InvDB: WDR33
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for WDR33 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for WDR33:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for WDR33 Antibodies for WDR33
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