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WDHD1 Gene

protein-coding   GIFtS: 51

GC14M054477
WD repeat and HMG-box DNA binding protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AND-1 1, 2
AND1 3
And-1 3
Descriptions
Acidic nucleoplasmic DNA-binding protein 1 2, 3
WD repeat and HMG-box DNA binding protein 1 2
External Ids
HGNC: 231701
Entrez Gene: 111692
UniProtKB: O757173
Ensembl: ENSG000001985547
Search outside databases for aliases for WDHD1 gene

Previous GC identifers: GC14M053397 GC14M053372

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for WDHD1:
The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high
mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may
function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and
cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin
assembly, transcription and replication. Alternative splicing results in two transcript variants
encoding different isoforms. [provided by RefSeq]

UniProtKB/Swiss-Prot: WDHD1_HUMAN, O75717
Function: Binds DNA with high affinity. May also be involved in protein-protein interactions (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WDHD1 gene  

Entrez Gene cytogenetic band: 14q22.3   Ensembl cytogenetic band:  14q22.3   HGNC cytogenetic band: 14q22.2

WDHD1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M054477:     (about GC identifiers)

Start:
54,476,692 bp from pter
End:
54,563,557 bp from pter
Size:
86,866 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: WDHD1_HUMAN, O75717 (See protein sequence)
Recommended Name: WD repeat and HMG-box DNA-binding protein 1  
Size: 1129 amino acids; 125967 Da
Subcellular location: Nucleus, nucleoplasm (By similarity)
PDB structures from and Proteopedia :
2D7L (3D)    

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001008397.1  NP_009017.1  


    ENSEMBL proteins: 
    ENSP00000352085 ENSP00000353793 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (WDHD1)
    Human Recombinant Proteins from Abnova (WDHD1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005654 nucleoplasm TAS9175701
    GO:0005730NOT nucleolus IDA18029348
    GO:0005737 cytoplasm TAS9175701
    About this table

    Antibodies for WDHD1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for WDHD1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (WDHD1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (WDHD1)
    Novus Biologicals Antibodies for WDHD1

    Assays for WDHD1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/7 InterPro domains/families (see all 7 ):
     IPR019782 WD40_repeat_2
     IPR019775 WD40_repeat_CS
     IPR000910 HMG_HMG1/HMG2
     IPR019781 WD40_repeat_sg
     IPR001680 WD40_repeat


       GeneDecks  WDHD1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O75717

    ProtoNet protein and cluster: O75717

    1 Blocks protein family: IPB001680 G-protein beta WD-40 repeat signature

    UniProtKB/Swiss-Prot: WDHD1_HUMAN, O75717
    Similarity: Contains 1 HMG box DNA-binding domain
    Similarity: Contains 7 WD repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDHD1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WDHD1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001008396

                  Applied Biosystems Silencer® siRNAs for WDHD1

                  Sigma-Aldrich siRNA for WDHD1  
                         Sigma-Aldrich shRNA for WDHD1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001008396
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001008396
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001008396 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_007086

    UniProtKB/Swiss-Prot: WDHD1_HUMAN, O75717
    Function: Binds DNA with high affinity. May also be involved in protein-protein interactions (By
    similarity)

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding TAS9175701
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  WDHD1 


    1 Interacting protein for WDHD1 (ENSP000003537933) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MCM10ENSP000003549453STRING (score=.926)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for WDHD1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDHD1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WDHD1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001008396

                  Sigma-Aldrich siRNA for WDHD1  
                         Sigma-Aldrich shRNA for WDHD1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001008396  NM_007086  

    REFSEQ mRNAs for WDHD1 gene (2 alternative transcripts): 

    NM_001008396.1   NM_007086.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001008396  NM_007086  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001008396
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001008396
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001008396 

    Additional cDNA sequence: 

    AJ006266.1 AK001538.1 AK001585.1 AK001620.1 AK293005.1 AY517556.1 BC000622.2 BC005040.1 
    BC043349.1 BC063041.1 

    11 DOTS entries:

    DT.107598  DT.97817273  DT.120786586  DT.101980015  DT.86857255  DT.97793240  DT.100023279  DT.440168 
    DT.95365607  DT.95365608  DT.99931138 

    24/112 AceView cDNA sequences (see all 112 ):

    AI203233 BQ439066 AL709945 BM978997 AA315413 BE274400 AW613020 AK001538 
    BU509101 BC000622 BC063041 CB122476 AI126514 AI339309 CF529821 AA732499 
    AW500820 AI275133 BC043349 BC005040 BQ012014 BE046969 BQ435406 AI080213 

    highest scoring ESTs for WDHD1:

    AA315413 AJ006266 AW961190 BC000622 BC005040 BC043349 BC063041 BE274400 BE275392 BE386196 

    Unigene Cluster for WDHD1:

    WD repeat and HMG-box DNA binding protein 1
    Hs.385998  [show with all ESTs]
    Unigene Representative Sequence: NM_007086


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000359167  ENST00000360586  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    WDHD1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for WDHD1

    1 / 2 / 3

    7 probe-sets matching WDHD1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    40355_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AJ006266 0.80 1.00 0.91 1

    216228_s_at2, 3 U133-A 1 1.00 1.00 -- -- AK001538 0.40 1.00 0.76 1

    204727_at2, 3 U133-A 1 1.00 1.00 -- -- AW772140 0.60 1.00 0.82 1

    204728_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_007086 0.60 1.00 0.82 1

    216228_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    204727_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    204728_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATTTTGTGT

    SOURCE GeneReport for Unigene cluster: Hs.385998

    Expression variation in blood from EXPOLDB for WDHD1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for WDHD1 gene from 5/15 species (see all 15 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    WDHD11   -- WD repeat and HMG-box DNA binding protein 1 91.63(n)
    90.06(a)
    490701  XM_547823.2  XP_547823.2 
    chimpanzee
    (Pan troglodytes)
    WDHD11   -- WD repeat and HMG-box DNA binding protein 1 99.73(n)
    99.47(a)
    452918  XM_001161828.1  XP_001161828.1 
    cow
    (Bos taurus)
    WDHD11   -- WD repeat and HMG-box DNA binding protein 1 91.08(n)
    89.24(a)
    540681  XM_612615.3  XP_612615.3 
    rat
    (Rattus norvegicus)
    Wdhd11   -- WD repeat and HMG-box DNA binding protein 1 83.9(n)
    82.44(a)
    305827  XM_223933.4  XP_223933.4 
    mouse
    (Mus musculus)
    Wdhd11, 5 145
    WD repeat and HMG-box DNA binding protein 11, 5 85(n)1
    83.89(a)1
    2189731  NM_172598.21  NP_766186.21 
     AK0363905  AK0424435  (see all 11)
    About this table        Species with no ortholog for WDHD1

    ENSEMBL Gene Tree for WDHD1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/334 NCBI SNPs in WDHD1 are shown (see all 334 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 93)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 14 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs71467751,2
    A,C,F,H54477507(+) AAGACG/ATCATC 2 -- ut31 ese37Minor allele frequency- A:0.07EU EA WA NA 560
    rs171281161,2
    C,F,H54527790(+) GTGGTA/GGATTG 2 L/P mis111Minor allele frequency- G:0.04NA EU EA WA 968
    rs71471471,2
    C,F,H54477694(+) AATTGG/TTTGGA 2 -- ut3111Minor allele frequency- T:0.07EU EA WA NA 980
    rs80200321,2
    C,F,H54532212(+) TAGAAA/GATCAC 2 F/L mis18Minor allele frequency- G:0.01EU EA WA 840
    rs171280821,2
    C,F,H54477023(+) TTAGTC/GTCTGC 2 -- ut31 ese311Minor allele frequency- G:0.05NA EU EA WA 980
    rs13541,2
    F,H54476742(+) tctccG/Aaacct 2 -- ut31 ese35Minor allele frequency- A:0.07EU EA WA 426
    rs71512851,2
    F,H54477742(+) AAAGGC/TCCTGC 2 -- ut314Minor allele frequency- T:0.01EU EA WA 418
    rs74929581,2
    H54477282(+) AAATTA/GTGGTA 2 -- ut314Minor allele frequency- G:0.00EU EA WA 420
    rs21830851,2
    A,C,F,H,O54542141(-) ACCTCT/GATGTC 2 -- int17Minor allele frequency- G:0.38EU EA WA NA 554
    rs28781741,2
    A,C,F,H,O54540374(+) GTCTTT/CGCTCA 2 -- int18Minor allele frequency- C:0.40EU EA WA NA 628
    About this table

    HapMap Linkage Disequilibrium images for WDHD1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 608126

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for WDHD1 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 11169 HGNC: 23170 AceView: WDHD1 Ensembl:ENSG00000198554 euGenes: HUgn11169
    ECgene: WDHD1 H-InvDB: WDHD1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for WDHD1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for WDHD1-related products

     Millipore Custom Antibody & Bulk Services
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     Predesigned and custom siRNAs for WDHD1 Antibodies for WDHD1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for WDHD1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for WDHD1

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