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WDFY2 Gene

protein-coding GIFtS: 46

GC13P051056

WD repeat and FYVE domain containing 2
(Previous name: WD40 and FYVE domain containing 2 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
OTTHUMP00000018795 ^²
PROF ^²
RP11-147H23.1 ^²
WDF2 ^², ³
ZFYVE22 ^¹, ², ³

Descriptions
WD repeat and FYVE domain containing 2 ^²
WD40 and FYVE domain containing 2 ^¹, ²
WD40- and FYVE domain-containing protein 2 ^³
Zinc finger FYVE domain-containing protein 22 ^³
propeller-FYVE protein ^²

External Ids
HGNC: 20482¹
Entrez Gene: 115825²
UniProtKB: Q96P53³
Ensembl: ENSG00000139668⁷

Search outside databases for aliases for WDFY2 gene

Previous GC identifers: GC13P046146 GC13P051095 GC13P049956

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for WDFY2:

This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The

function of this gene is unknown. An alternatively spliced transcript variant of this gene may

exist. [provided by RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the WDFY2 gene

Entrez Gene cytogenetic band: 13q14.3 Ensembl cytogenetic band: 13q14.3 HGNC cytogenetic band: 13q14.12

WDFY2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13 GeneLoc Exon Structure

GeneLoc location for GC13P051056: (about GC identifiers)

Start:	51,056,485 bp from pter
End:	51,234,172 bp from pter
Size:	177,688 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000013.9 NT_024524.13

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: WDFY2_HUMAN, Q96P53 (See protein sequence)

Recommended Name: WD repeat and FYVE domain-containing protein 2

Size: 400 amino acids; 45154 Da

Secondary accessions: Q96CS1

REFSEQ proteins: NP_443182.1

ENSEMBL proteins:

ENSP00000298125

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (WDFY2)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for WDFY2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Monoclonal and Polyclonal Antibodies from Abnova (WDFY2)
	Novus Biologicals Antibody for WDFY2

Assays for WDFY2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q96P53

ProtoNet protein and cluster: Q96P53

1 Blocks protein family: IPB000306 Zn-finger

UniProtKB/Swiss-Prot: WDFY2_HUMAN, Q96P53

Similarity: Contains 1 FYVE-type zinc finger

Similarity: Contains 7 WD repeats

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (WDFY2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (WDFY2)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052950

Applied Biosystems Silencer® siRNAs for WDFY2

Sigma-Aldrich siRNA for WDFY2

Sigma-Aldrich shRNA for WDFY2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for WDFY2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_052950
Myc/DDK tagged cDNA clone in CMV expression vector: NM_052950
untagged cDNA clones in CMV expression vector (see all 2): NM_052950

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_052950

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008270	zinc ion binding	IEA	--
GO:0046872	metal ion binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Gene Network Central^TM Interacting Genes and Proteins Network for WDFY2

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for WDFY2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (WDFY2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (WDFY2)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052950

Sigma-Aldrich siRNA for WDFY2

Sigma-Aldrich shRNA for WDFY2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_052950

REFSEQ mRNAs for WDFY2 gene:

NM_052950.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_052950

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_052950
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_052950
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_052950

Additional cDNA sequence:

AF411978.1 AK054833.1 AK058149.1 AK093224.1 AK309815.1 AK311177.1 BC014004.2 CR612847.1

17 DOTS entries:

DT.312154 DT.95343339 DT.100022742 DT.97816844 DT.100660634 DT.100751290 DT.312155 DT.120769969

DT.40238189 DT.86838664 DT.120769966 DT.120769981 DT.40132095 DT.75148371 DT.91927360 DT.95370092

DT.99932067

24/137 AceView cDNA sequences (see all 137 ):

BX099703 AI417133 AW614396 AA864300 NM_052950 T87541 BU616176 AA004280

BQ888546 BC014004 BX644610 BQ431026 AA703241 CB217004 AA701982 BQ574560

BM672061 CD358198 CR612847 BU689194 AI057330 T87542 BU102054 BU520776

highest scoring ESTs for WDFY2:

AA703241 AA845630 AA701982 AA716631 AA864300 AF411978 AI057330 AI240613 AK058149 AL562744

Unigene Cluster for WDFY2:

WD repeat and FYVE domain containing 2

Hs.208550 [show with all ESTs]

Unigene Representative Sequence: NM_052950

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000298125

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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WDFY2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for WDFY2

¹ / ² / ³

4 probe-sets matching WDFY2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
58323_at²^{, 3}	U95-B	1	1.00	1.00	0.96	0.71	AA703241	1.00	1.00	1.00	1
75991_at²^{, 3}	U95-D	1	0.94	1.00	0.61	1.29	AA845630	1.00	1.00	1.00	1
227490_at²^{, 3}	U133-B	1	1.00	1.00	--	--	T87542	0.80	1.00	0.91	1
227490_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks WDFY2 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TGCACAGTGG

SOURCE GeneReport for Unigene cluster: Hs.208550

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for WDFY2 gene from 5/10 species (see all 10 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	WDFY2¹	--	WD repeat and FYVE domain containing 2	90.56(n) 95.56(a)	606922 XM_843402.1 XP_848495.1
chimpanzee (Pan troglodytes)	WDFY2¹	--	WD repeat and FYVE domain containing 2	99.67(n) 99.5(a)	452730 XM_001158979.1 XP_001158979.1
rat (Rattus norvegicus)	Wdfy2¹	--	WD repeat and FYVE domain containing 2	91.75(n) 97.75(a)	305956 XM_224296.4 XP_224296.3
mouse (Mus musculus)	Wdfy2^{1, 5}	14⁵	WD repeat and FYVE domain containing 2^{1, 5}	92.42(n)¹ 98(a)¹	268752¹ NM_175546.3¹ NP_780755.1¹ AA171276⁵ AK031001⁵ (see all 14)
chicken (Gallus gallus)	WDFY2¹	--	WD repeat and FYVE domain containing 2	84.25(n) 93(a)	770107 XM_001232665.1 XP_001232666.1

About this table Species with no ortholog for WDFY2

ENSEMBL Gene Tree for WDFY2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for WDFY2 gene

WDFY1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/488 NCBI SNPs in WDFY2 are shown (see all 488 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 170)

AB	SNP ID	Valid	Chr 13 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs12856726^1,2	C,F,H	51232620(+)	`CTGCTC/TCCAAC`	1	--	ut3¹		4	EU EA WA	420
	rs9535753^1,2	F,H	51233202(+)	`CCCTCT/CGTATT`	1	--	ut3¹		4	EU EA WA	420
	rs2296029^1,2	C,F	51233766(+)	`AAAGCC/TGTTTT`	1	--	ut3¹		4	EA NA	1610
	rs4542553^1,2	H	51233245(+)	`TTATCC/ATAATG`	1	--	ut3¹		4	EU EA WA	418
	rs4436689^1,2	H	51232894(+)	`gcaacC/Ataaat`	1	--	ut3¹		4	EU EA WA	418
	rs9596568^1,2	H	51232412(+)	`TCCCAG/TTGTTT`	1	--	ut3¹		4	EU EA WA	416
	rs1885854^1,2	C,F,H,O	51211196(+)	`GTGACC/TGCTCT`	1	T/T	syn¹ ese³		8	NA EU EA WA	632
	rs1923874^1,2	A,C,F,H,O	51161530(-)	`AATTCC/TTCCTC`	1	--	int¹		15	NA EU EA WA	1342
	rs9535717^1,2	A,C,F,H	51083939(+)	`AGGAGA/GTACCT`	1	--	int¹ trp³		4	EU EA WA	418
	rs4555048^1,2	A,C,F,H	51083856(+)	`TTGCCA/GGGGGT`	1	--	int¹		4	EU EA WA	418

About this table

HapMap Linkage Disequilibrium images for WDFY2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610418

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for WDFY2 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis. (PubMed id 16873553)^{1, 3} Hayakawa A....Corvera S. (2006)
WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta. (PubMed id 17313651)³ Fritzius T....Moelling K. (2007)
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)³ Kimura K.... Sugano S. (2006)
A WD-FYVE protein binds to the kinases Akt and PKCzeta/lambda. (PubMed id 16792529)³ Fritzius T....Moelling K. (2006)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)³ Ota T....Sugano S. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 115825	HGNC: 20482	AceView: WDFY2	Ensembl:ENSG00000139668	euGenes: HUgn115825
ECgene: WDFY2	H-InvDB: WDFY2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for WDFY2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for WDFY2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for WDFY2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for WDFY2		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for WDFY2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (WDFY2)
	Antibodies (WDFY2)
	Chimera RNAi (WDFY2)
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

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Search OriGene for WDFY2

Search Tocris compounds for WDFY2

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for WDFY2

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WDFY2 Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for WDFY2

Summaries
for WDFY2

Genomic Location
for WDFY2

Proteins
for WDFY2

Protein Domains/
Families
for WDFY2

Gene Function
for WDFY2

Pathways & Interactions
for WDFY2

Drugs & Compounds
for WDFY2

Transcripts
for WDFY2

Expression
for WDFY2

Orthologs
for WDFY2

Paralogs
for WDFY2

SNPs/Variants
for WDFY2

Disorders & Mutations
for WDFY2

Medical News
for WDFY2

Publications
for WDFY2

Search for WDFY2

Genome Databases showing WDFY2

Other Databases showing WDFY2

Specialized Databases showing WDFY2

Licensable Technologies
for WDFY2

Services
for WDFY2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

WDFY2 Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor WDFY2

Summariesfor WDFY2

Genomic Locationfor WDFY2

Proteinsfor WDFY2

Protein Domains/Familiesfor WDFY2

Gene Functionfor WDFY2

Pathways & Interactionsfor WDFY2

Drugs & Compoundsfor WDFY2

Transcriptsfor WDFY2

Expression for WDFY2

Orthologsfor WDFY2

Paralogsfor WDFY2

SNPs/Variantsfor WDFY2

Disorders & Mutationsfor WDFY2

Medical Newsfor WDFY2

Publicationsfor WDFY2

Search for WDFY2

Genome Databases showing WDFY2

Other Databases showing WDFY2

Specialized Databases showing WDFY2

Licensable Technologiesfor WDFY2

Servicesfor WDFY2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for WDFY2

Summaries
for WDFY2

Genomic Location
for WDFY2

Proteins
for WDFY2

Protein Domains/
Families
for WDFY2

Gene Function
for WDFY2

Pathways & Interactions
for WDFY2

Drugs & Compounds
for WDFY2

Transcripts
for WDFY2

Expression
for WDFY2

Orthologs
for WDFY2

Paralogs
for WDFY2

SNPs/Variants
for WDFY2

Disorders & Mutations
for WDFY2

Medical News
for WDFY2

Publications
for WDFY2

Licensable Technologies
for WDFY2

Services
for WDFY2