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WDFY1 Gene

protein-coding   GIFtS: 50

GC02M224448
WD repeat and FYVE domain containing 1
(Previous name: WD40 and FYVE domain containing 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FENS-1 1, 2, 3
KIAA1435 1, 3
WDF1 1, 2, 3
ZFYVE17 1, 2, 3
Descriptions
Phosphoinositide-binding protein 1 3
WD repeat and FYVE domain containing 1 2
WD40 and FYVE domain containing 1 1, 2
WD40- and FYVE domain-containing protein 1 3
Zinc finger FYVE domain-containing protein 17 3
phosphoinositide-binding protein SR1 2
External Ids
HGNC: 204511
Entrez Gene: 575902
UniProtKB: Q8IWB73
Ensembl: ENSG000000854497
Search outside databases for aliases for WDFY1 gene

Previous GC identifers: GC02M223461 GC02M224704 GC02M224942 GC02M224565

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for WDFY1:
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell
signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. This gene encodes
a protein which contains a single FYVE domain and multiple WD40 repeats. This protein is localized
to endosomes. [provided by RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WDFY1 gene  

Entrez Gene cytogenetic band: 2q36.1   Ensembl cytogenetic band:  2q36.1   HGNC cytogenetic band: 2q36.2

WDFY1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M224448:     (about GC identifiers)

Start:
224,448,309 bp from pter
End:
224,518,296 bp from pter
Size:
69,988 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: WDFY1_HUMAN, Q8IWB7 (See protein sequence)
Recommended Name: WD repeat and FYVE domain-containing protein 1  
Size: 410 amino acids; 46324 Da
Subunit: Binds PtdIns3P in vitro with high specificity over other phosphoinositides
Subcellular location: Early endosome
Secondary accessions: Q9H9D5 Q9P2B3

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_065881.1  

    ENSEMBL proteins: 
    ENSP00000233055 ENSP00000272881 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (FENS1)
    Human Recombinant Proteins from Abnova (WDFY1)
                  OriGene Purified Recombinant Human Protein: WDFY1 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA11739631
    GO:0005769 early endosome IDA11739631
    GO:0005829 cytosol IDA11739631
    About this table

    Antibodies for WDFY1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (FENS1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (WDFY1)
    Novus Biologicals Antibody for WDFY1

    Assays for WDFY1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR019782 WD40_repeat_2
     IPR019775 WD40_repeat_CS
     IPR000306 Znf_FYVE
     IPR019781 WD40_repeat_sg
     IPR001680 WD40_repeat


       GeneDecks  WDFY1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8IWB7

    ProtoNet protein and cluster: Q8IWB7

    1 Blocks protein family: IPB000306 Zn-finger

    UniProtKB/Swiss-Prot: WDFY1_HUMAN, Q8IWB7
    Similarity: Contains 1 FYVE-type zinc finger
    Similarity: Contains 7 WD repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDFY1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WDFY1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020830

                  Applied Biosystems Silencer® siRNAs for WDFY1

                  Sigma-Aldrich siRNA for WDFY1  
                         Sigma-Aldrich shRNA for WDFY1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for WDFY1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020830
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020830
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020830 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_020830

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005545 phosphatidylinositol binding IDA11739631
    GO:0008270 zinc ion binding NAS11739631
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008150 biological_process ND--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for WDFY1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WDFY1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WDFY1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020830

                  Sigma-Aldrich siRNA for WDFY1  
                         Sigma-Aldrich shRNA for WDFY1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_020830  

    REFSEQ mRNAs for WDFY1 gene: 

    NM_020830.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_020830  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020830
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020830
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020830 

    Additional cDNA sequence: 

    AB037856.1 AF411977.1 AJ310568.1 AK022888.1 AK023415.1 BC040525.1 BC065934.1 

    7 DOTS entries:

    DT.415418  DT.91770521  DT.95150414  DT.100027471  DT.120940389  DT.91770519  DT.100034723 

    24/276 AceView cDNA sequences (see all 276 ):

    AF411977 AA450337 BG177582 BF447259 AA721432 BG759326 AW070738 AA411290 
    AW243572 BG109779 CA398252 CD171698 BU167040 BM470844 BG119034 BM541399 
    AK023415 BM786152 NM_020830 BP364649 AA406307 BM692877 BF223120 BX115673 

    highest scoring ESTs for WDFY1:

    AB037856 AF411977 AI695418 AJ310568 AK022888 AL036048 AW963897 BC040525 BC065934 BE262784 

    Unigene Cluster for WDFY1:

    WD repeat and FYVE domain containing 1
    Hs.708107  [show with all ESTs]
    Unigene Representative Sequence: BC040525


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for WDFY1

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11 ^ 12
    SP1:                                            -     -                 -                                             
    SP2:                          -     -           -     -                 -                                             
    SP3:                                                                                                                  
    SP4:                                                                    -                                             
    SP5:                                                                                                                  

    About this scheme

    ECgene alternative splicing isoforms for WDFY1

    2 Ensembl transcripts including schematic representations:
    ENST00000233055  ENST00000352510  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    WDFY1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for WDFY1

    1 / 2 / 3

    6 probe-sets matching WDFY1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    64936_r_at2, 3 U95-C 1 1.00 1.00 0.74 0.71 AI547017 0.60 1.00 0.82 1

    64393_at2, 3 U95-C 1 1.00 1.00 0.77 1.29 AI695418 0.80 0.88 0.84 1

    224800_at2, 3 U133-B 1 1.00 1.00 -- -- AK022888 0.80 0.88 0.84 1

    233559_s_at2, 3 U133-B 1 1.00 1.00 -- -- AK023415 0.60 1.00 0.82 1

    224800_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    233559_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTATATTTAT

    SOURCE GeneReport for Unigene cluster: Hs.708107
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for WDFY1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    WDFY11   -- WD repeat and FYVE domain containing 1 87.66(n)
    95.3(a)
    488552  XM_545670.2  XP_545670.2 
    chimpanzee
    (Pan troglodytes)
    WDFY11   -- WD repeat and FYVE domain containing 1 99.27(n)
    99.51(a)
    744585  XM_001166904.1  XP_001166904.1 
    cow
    (Bos taurus)
    WDFY11   -- WD repeat and FYVE domain containing 1 90.98(n)
    97.8(a)
    614729  NM_001046542.1  NP_001040007.1 
    mouse
    (Mus musculus)
    Wdfy15 1 WD repeat and FYVE domain containing 1   -- BQ031098 
    chicken
    (Gallus gallus)
    WDFY11   -- WD repeat and FYVE domain containing 1 81.78(n)
    91.91(a)
    424806  NM_001031310.1  NP_001026481.1 
    About this table        Species with no ortholog for WDFY1

    ENSEMBL Gene Tree for WDFY1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for WDFY1 gene
    WDFY22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/508 NCBI SNPs in WDFY1 are shown (see all 508 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 137)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs6664381,2
    A,C,F,H,O224448949(+) CAATAA/GTATTT 1 -- ut318Minor allele frequency- G:0.24MN NA EU EA WA 736
    rs6477291,2
    A,C,F,H,O224447946(+) ACTAGA/CTAGCT 1 -- ng518Minor allele frequency- C:0.34EU EA WA 838
    rs5711111,2
    A,C,F,H224451617(-) TGGGCG/AGCGTC 1 -- ut31 ese36Minor allele frequency- A:0.37EU EA WA NA 594
    rs4964711,2
    C,F,H,O224450047(+) AAGTCA/TATGTT 1 -- ut3113Minor allele frequency- T:0.12NS MN NA EU EA WA 848
    rs6787461,2
    A,C,F,H224450237(+) TGGTAC/TGATCC 1 -- ut31 ese310Minor allele frequency- T:0.15NS MN EU EA WA 710
    rs67408771,2
    A,C,F,H224448006(+) CAAGCG/AGCAGA 1 -- ng517Minor allele frequency- A:0.12EU EA WA NA 560
    --
    rs31877271,2
    C,F,H224448576(-) CTCTTT/AGAGTA 1 -- ut31 ese38Minor allele frequency- A:0.06MN NA EU EA WA 742
    rs104981551,2
    C,F,H224449644(+) GCTATG/CGAGTA 1 -- ut31 ese316Minor allele frequency- C:0.13NS EU EA WA NA 1088
    rs14388321,2
    C,F,H224520196(+) TGGTTT/CTTGTG 1 -- ng315Minor allele frequency- C:0.17MN EU EA WA 604
    rs104981561,2
    C,F,H224449886(+) TGCTTG/AAACAA 1 -- ut3112Minor allele frequency- A:0.14NS EU EA WA NA 662
    About this table

    HapMap Linkage Disequilibrium images for WDFY1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    5 PubMed articles for WDFY1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 57590 HGNC: 20451 AceView: WDFY1andAP1S3 Ensembl:ENSG00000085449 euGenes: HUgn57590
    ECgene: WDFY1 H-InvDB: WDFY1
    (According to HUGE)
    About This Section

    HUGE: KIAA1435
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for WDFY1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for WDFY1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for WDFY1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Search for Antibodies & Assays

     Recombinant Proteins
    (FENS1)
     Antibodies (FENS1)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for WDFY1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for WDFY1

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