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Aliases & Descriptions for WASL
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| CR16 5 | | DKFZp779G0847 2 | | MGC48327 2 | | N-WASP 1, 2, 3 | | NWASP 1, 2 | | WIPF3 5 |
| | | Descriptions |
|---|
| Wiskott-Aldrich syndrome gene-like 2 | | Wiskott-Aldrich syndrome gene-like protein 2 | | Wiskott-Aldrich syndrome-like 2 | | neural Wiskott-Aldrich syndrome protein 2 |
|
| | Search outside databases for aliases for WASL genePrevious GC identifers: GC07M121799 GC07M122862 GC07M122876 GC07M122915 |
Summaries for WASL(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for WASL: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq] UniProtKB/Swiss-Prot: WASL_HUMAN, O00401Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expressionGene Wiki entry for WASL |
Genomic Location for WASL
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the WASL gene 
Entrez Gene cytogenetic band: 7q31.3 Ensembl cytogenetic band: 7q31.32 HGNC cytogenetic band: 7q31.3WASL Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07M123109:
(about GC identifiers)
Start:
|
123,109,233 bp from pter |
End:
|
123,176,352 bp from pter |
Size:
|
67,120 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr7-,CRA_TCAG 122,710,624-122,777,740 |
RefSeq DNA sequence:- NC_000007.12 NT_007933.14 NT_079596.2
| Proteins for WASL
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: WASL_HUMAN, O00401 (See
protein sequence)Recommended Name: Neural Wiskott-Aldrich syndrome protein Size: 505 amino acids; 54827 Da
Subunit: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11 (By similarity). Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly
Subcellular location: Cytoplasm, cytoskeleton (By similarity). Nucleus (By similarity). Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity)
PDB structure from and Proteopedia :2FF3 (3D)
 2VCP (3D)
 
Secondary accessions: Q7Z746Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_003932.3
ENSEMBL proteins: ENSP00000223023
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: WASL 
5 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for WASL: Assays for WASL: | Protein
Domains/ Families for WASL(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry O00401
ProtoNet protein and cluster: O00401 1 Blocks protein family: IPB003124 Actin-binding WH2
UniProtKB/Swiss-Prot: WASL_HUMAN, O00401Similarity: Contains 1 CRIB domainSimilarity: Contains 1 WH1 domainSimilarity: Contains 2 WH2 domains | Gene Function for WASL
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003941
Applied Biosystems Silencer® siRNAs for WASL
Sigma-Aldrich siRNA for WASL  Sigma-Aldrich shRNA Panels and shRNA for WASL  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003941                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003941                                  untagged cDNA clone in CMV expression vector: NM_003941 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_003941
UniProtKB/Swiss-Prot: WASL_HUMAN, O00401Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expressionGenatlas biochemistry entry for WASL:Wiskott-Aldrich syndrome protein-like,64kDa,neural,concentrated at the nerve terminal region,inducing long actin microspikes when coexpressed with active CDC4211 MGI mutant phenotypes (inferred from 5 alleles ) (MGI details for Wasl):
3 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for WASL
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
5/8 Sigma-Aldrich "Your Favorite Gene" Pathways for WASL (Your Favorite Gene powered by Ingenuity) (see all 8
) 
Gene Network CentralTM Interacting Genes and Proteins Network for WASL 
5/78 Interacting proteins for WASL (O004011, 2 ENSP000002230233) via UniProtKB, MINT, and/or STRING (see all 78
)About this table
5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6
): About this table
|
Drugs & Compounds for WASL(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for WASL 10 Novoseek chemical compound relationships for WASL gene
| Compound |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| wiskostatin |
95.26 |
12 |
17405146 (2), 15235593 (2), 18667055 (2), 15800060 (1) (see all 7) |
| pip2 |
61.97 |
8 |
8895577 (2), 12147689 (2), 11792818 (1), 17302440 (1) (see all 6) |
| tyrosine |
31.85 |
19 |
12431372 (4), 8895577 (2), 17474908 (2), 14676198 (2) (see all 10) |
| proline |
25.22 |
3 |
17229736 (1), 12147689 (1), 10973991 (1) |
| phosphoinositide |
24.80 |
4 |
16949824 (1), 10953003 (1), 11865026 (1) |
| phosphatidylinositol |
12.78 |
5 |
11694514 (2), 11052943 (1), 18923421 (1), 10219243 (1) |
| glucose |
4.05 |
2 |
11694514 (1), 12456725 (1) |
| lipid |
0.00 |
5 |
17988218 (2), 12477732 (2), 17302440 (1) |
| latex |
0.00 |
2 |
11401525 (1), 12551957 (1) |
| adp |
0.00 |
3 |
11792818 (2) |
About this table
|
Transcripts for WASL(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003941
Sigma-Aldrich siRNA for WASL  Sigma-Aldrich shRNA Panels and shRNA for WASL  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_003941 REFSEQ mRNAs for WASL gene: NM_003941.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_003941               OriGene GFP tagged cDNA clone in CMV expression vector: NM_003941                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003941                                  untagged cDNA clone in CMV expression vector: NM_003941  Additional cDNA sequence: AK131016.1 BX640852.1 CR591885.1 10 DOTS entries: DT.218480 DT.75117246 DT.95268724 DT.121053298 DT.65287633 DT.121053301 DT.121053311 DT.75118576 DT.92421825 DT.100701098 24/159 AceView cDNA sequences (see all 159
):CB127157 W73531 BE891172 BQ717939 CN484199 AI969375 BU952498 AI521769 BU951055 AI653277 BQ286524 CD107134 CB132489 CD359638 AI620950 BQ425087 BE504979 AW044456 BM751087 N34978 AI352141 BU902219 CB123879 BQ269867
highest scoring ESTs for WASL:D88460 AA448782 AA625268 AA860954 AI261600 AI459169 AI478180 AI478447 AI742750 AI760111 Unigene Clusters for WASL: Wiskott-Aldrich syndrome-like Hs.143728 [show with all ESTs], Hs.708402 [show with all ESTs]Unigene Representative Sequences: NM_003941, BX640852
GeneLoc Exon Structure
1 Ensembl transcript including schematic representation: ENST00000223023
|
Expression for WASL
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| WASL expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for WASL
1 / 2 / 3 9 probe-sets matching WASL gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene clusters: Hs.143728 Hs.708402
Expression variation in blood from EXPOLDB for WASL |
Orthologs for WASL
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for WASL gene from 5/12 species (see all 12
)
About this table Species with no ortholog for WASL
ENSEMBL Gene Tree for WASL | Paralogs for WASL(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for WASL gene
- WAS2
|
SNPs/Variants for WASL(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for WASL (up to first 250kb)
|
Disorders & Mutations for WASL
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 612432
9 Novoseek disease relationships for WASL gene
| Disease |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| wiskott-aldrich syndrome |
95.50 |
66 |
11952639 (2), 15085951 (2), 17092940 (2), 11432863 (2) (see all 54) |
| scar1 |
87.03 |
8 |
11747816 (6), 14561714 (1), 11029465 (1) |
| scar |
40.05 |
6 |
12872157 (1), 10940259 (1), 11747836 (1), 15362118 (1) (see all 6) |
| cancer |
1.98 |
1 |
17985201 (1) |
| shock |
0.00 |
4 |
12871950 (2), 15791211 (1), 17535252 (1) |
| cystic fibrosis |
0.00 |
1 |
17306384 (1) |
| carcinoma |
0.00 |
7 |
15084285 (2), 15362118 (1), 18362183 (1) |
| tumors |
0.00 |
5 |
17092940 (3), 15699051 (1) |
| metastasis |
0.00 |
1 |
17985201 (1) |
About this table
|
Medical News for WASL(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for WASL (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/185 PubMed articles for WASL gene (see all 185
):- Identification of N-WASP homologs in human and rat brain. (PubMed id 9322739)1, 2, 3, 4 Fukuoka M....Takenawa T. (1997)
- Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP. (PubMed id 9422512)1, 2, 3 Miki H....Takenawa T. (1998)
- The structural basis of actin interaction with multiple WH2/beta- thymosin motif-containing proteins. (PubMed id 16531231)1, 3, 4 Aguda A.H.... Robinson R.C. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- The DNA sequence of human chromosome 7. (PubMed id 12853948)3, 4 Hillier L.W.... Wilson R.K. (2003)
- Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility. (PubMed id 10491394)3, 4 Egile C.... Carlier M.-F. (1999)
- Neural Wiskott-Aldrich syndrome protein is implicated in the actin- based motility of Shigella flexneri. (PubMed id 9582270)3, 4 Suzuki T.... Sasakawa C. (1998)
- Translocation of N-WASP by nuclear localization and export signals into the nucleus modulates expression of HSP90. (PubMed id 12871950)1, 3 Suetsugu S. and Takenawa T. (2003)
- Interaction of HSP90 to N-WASP leads to activation and protection from proteasome-dependent degradation. (PubMed id 15791211)1, 3 Park S.J....Takenawa T. (2005)
- N-WASP is a putative tumour suppressor in breast cancer cells, in vitro and in vivo, and is associated with clinical outcome in patients with breast cancer. (PubMed id 17985201)1, 3 Martin T.A....Jiang W.G. (2008)
|
Search for WASL
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing WASL
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing WASL
(According to HUGE)
About This Section
| -- |
Specialized Databases showing WASL(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for WASL(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for WASL:

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|
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| Antibodies & Assays for WASL  (N-WASP) |
| | | | Search Tocris compounds for WASL |
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Incremental update: 13 Oct 2009
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