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Aliases for WASH2P Gene

Aliases for WASH2P Gene

  • WAS Protein Family Homolog 2 Pseudogene 2 3
  • Family With Sequence Similarity 39, Member B 2 3
  • FAM39B 3 4
  • CXYorf1-Like Protein On Chromosome 2 4
  • Protein FAM39B 4

External Ids for WASH2P Gene

Previous HGNC Symbols for WASH2P Gene

  • FAM39B

Previous GeneCards Identifiers for WASH2P Gene

  • GC02P114060
  • GC02P114341

Summaries for WASH2P Gene

GeneCards Summary for WASH2P Gene

WASH2P (WAS Protein Family Homolog 2 Pseudogene) is a Pseudogene.

UniProtKB/Swiss-Prot for WASH2P Gene

  • Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for WASH2P Gene

Genomics for WASH2P Gene

Regulatory Elements for WASH2P Gene

Genomic Location for WASH2P Gene

Start:
113,583,653 bp from pter
End:
113,599,043 bp from pter
Size:
15,391 bases
Orientation:
Plus strand

Genomic View for WASH2P Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for WASH2P Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for WASH2P Gene

Proteins for WASH2P Gene

  • Protein details for WASH2P Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q6VEQ5-WASH2_HUMAN
    Recommended name:
    WAS protein family homolog 2
    Protein Accession:
    Q6VEQ5
    Secondary Accessions:

    Protein attributes for WASH2P Gene

    Size:
    465 amino acids
    Molecular mass:
    50312 Da
    Quaternary structure:
    • Component of the WASH core complex also known as WASH regulatory complex SHRC composed of WASH (WASH1, WASH2P or WASH3P), FAM21 (FAM21A or FAM21C), KIAA1033/SWIP, KIAA0196/Strumpellin and CCDC53. The WASH core complex associates with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex. Interacts (via WHD1 region) with FAM21C; the interaction is direct. Interacts with alpha-tubulin. Interacts with BECN1; WASH1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2). Interacts with EXOC1, EXOC4, EXOC8; in MMP14-positive endosomes in breast tumor cells; indicative for an association with the exocyst complex (By similarity).
    Miscellaneous:
    • WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (PubMed:18159949). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.
    SequenceCaution:
    • Sequence=AL078621; Type=Erroneous termination; Positions=25; Note=Translated as Arg.; Evidence={ECO:0000305}; Sequence=AL078621; Type=Erroneous termination; Positions=180; Note=Translated as Glu.; Evidence={ECO:0000305};

neXtProt entry for WASH2P Gene

Proteomics data for WASH2P Gene at MOPED

Post-translational modifications for WASH2P Gene

No Post-translational modifications

No data available for DME Specific Peptides for WASH2P Gene

Domains for WASH2P Gene

Gene Families for WASH2P Gene

HGNC:
  • WASH :WAS protein homologs

Protein Domains for WASH2P Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for WASH2P Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q6VEQ5

UniProtKB/Swiss-Prot:

WASH2_HUMAN :
  • Q6VEQ5
Domain:
  • The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro.
  • Contains 1 WH2 domain.
Family:
  • Belongs to the WASH1 family.
genes like me logo Genes that share domains with WASH2P: view

Function for WASH2P Gene

Molecular function for WASH2P Gene

UniProtKB/Swiss-Prot Function: Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).
genes like me logo Genes that share phenotypes with WASH2P: view

Animal Models for WASH2P Gene

MGI Knock Outs for WASH2P:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for WASH2P

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , miRNA , Transcription Factor Targeting and HOMER Transcription for WASH2P Gene

Localization for WASH2P Gene

Subcellular locations from UniProtKB/Swiss-Prot for WASH2P Gene

Early endosome membrane. Recycling endosome membrane. Late endosome. Cytoplasmic vesicle, autophagosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Localization to the endosome membrane is mediated via its interaction with FAM21. {ECO:0000250 UniProtKB:A8K0Z3}.

No data available for Subcellular locations from COMPARTMENTS and Gene Ontology (GO) - Cellular Components for WASH2P Gene

Pathways for WASH2P Gene

SuperPathways for WASH2P Gene

No Data Available

Interacting Proteins for WASH2P Gene

Selected Interacting proteins: Q6VEQ5-WASH2_HUMAN for WASH2P Gene via MINT I2D

Gene Ontology (GO) - Biological Process for WASH2P Gene

None

No data available for Pathways by source for WASH2P Gene

Transcripts for WASH2P Gene

mRNA/cDNA for WASH2P Gene

Unigene Clusters for WASH2P Gene

WAS protein family homolog 2 pseudogene:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for WASH2P

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for WASH2P
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for WASH2P Gene

No ASD Table

Relevant External Links for WASH2P Gene

GeneLoc Exon Structure for
WASH2P
ECgene alternative splicing isoforms for
WASH2P

Expression for WASH2P Gene

mRNA expression in normal human tissues for WASH2P Gene

Integrated Proteomics: protein expression from ProteomicsDB and MOPED for WASH2P Gene

SOURCE GeneReport for Unigene cluster for WASH2P Gene Hs.446466

genes like me logo Genes that share expressions with WASH2P: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for WASH2P Gene

Orthologs for WASH2P Gene

This gene was present in the common ancestor of human and mouse.

Orthologs for WASH2P Gene

Organism Taxonomy Gene Similarity Type Details
mouse
(Mus musculus)
Mammalia Wash 16
Species with no ortholog for WASH2P:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • cow (Bos Taurus)
  • dog (Canis familiaris)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for WASH2P Gene

ENSEMBL:
Gene Tree for WASH2P (if available)
TreeFam:
Gene Tree for WASH2P (if available)

Paralogs for WASH2P Gene

Selected SIMAP similar genes for WASH2P Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with WASH2P: view

No data available for Paralogs for WASH2P Gene

Variants for WASH2P Gene

Sequence variations from dbSNP and Humsavar for WASH2P Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type MAF
rs20604 -- 113,587,130(+) AAGGG(A/G)AGGGA intron-variant
rs367650 -- 113,594,179(+) TGCAC(A/G)TGGAG intron-variant
rs413632 -- 113,587,280(+) TATTC(A/T)AGGCA intron-variant
rs421144 -- 113,589,826(+) AGGAT(C/T)CCACA intron-variant
rs424703 -- 113,585,696(+) AGAGG(A/G)ACAAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for WASH2P Gene

Variant ID Type Subtype PubMed ID
esv380 CNV CNV 17122850
essv901 CNV CNV 17122850
nsv821507 CNV Duplication 20802225
esv24774 CNV Gain 19812545
nsv10133 CNV Gain 18304495

Relevant External Links for WASH2P Gene

HapMap Linkage Disequilibrium report
WASH2P

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for WASH2P Gene

Disorders for WASH2P Gene

No disorders were found for WASH2P Gene.

No data available for UniProtKB/Swiss-Prot for WASH2P Gene

Publications for WASH2P Gene

  1. Human subtelomeric WASH genes encode a new subclass of the WASP family. (PMID: 18159949) Linardopoulou E.V. … Trask B.J. (PLoS Genet. 2007) 2 3 4
  2. Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region. (PMID: 10655549) Ciccodicola A. … D'Urso M. (Hum. Mol. Genet. 2000) 3 4
  3. Diverse fates of paralogs following segmental duplication of telomeric genes. (PMID: 15233989) Wong A. … Ledbetter D.H. (Genomics 2004) 3 4
  4. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PMID: 16344560) Kimura K. … Sugano S. (Genome Res. 2006) 3
  5. Characterization of the murine orthologue of a novel human subtelomeric multigene family. (PMID: 11701968) Gianfrancesco F. … D'Urso M. (Cytogenet. Cell Genet. 2001) 3

Products for WASH2P Gene

  • QIAGEN qRT-PCR Assays for microRNAs that regulate WASH2P
    • QuantiTect SYBR Green Assays in human,mouse,rat
    • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
    • QuantiFast Probe-based Assays in human,mouse,rat
    • Predesigned siRNA for gene silencing in human,mouse,rat for WASH2P
    • Block miRNA regulation of WASH2P using miScript Target Protectors

    Sources for WASH2P Gene

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