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Aliases for WASH1 Gene

Aliases for WASH1 Gene

  • WAS Protein Family Homolog 1 2 3
  • Family With Sequence Similarity 39, Member E 2 3
  • CXYorf1-Like Protein On Chromosome 9 3 4
  • FAM39E 3 4
  • WASH 3 6
  • FLJ00075 Protein 3
  • Protein FAM39E 4

External Ids for WASH1 Gene

Previous Symbols for WASH1 Gene

  • FAM39E

Summaries for WASH1 Gene

GeneCards Summary for WASH1 Gene

WASH1 (WAS Protein Family Homolog 1) is a Protein Coding gene. GO annotations related to this gene include actin binding and alpha-tubulin binding.

UniProtKB/Swiss-Prot for WASH1 Gene

  • Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for WASH1 Gene

Genomics for WASH1 Gene

Genomic Location for WASH1 Gene

Start:
14,511 bp from pter
End:
29,739 bp from pter
Size:
15,229 bases
Orientation:
Minus strand

Genomic View for WASH1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for WASH1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for WASH1 Gene

Regulatory Elements for WASH1 Gene

Proteins for WASH1 Gene

  • Protein details for WASH1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    A8K0Z3-WASH1_HUMAN
    Recommended name:
    WAS protein family homolog 1
    Protein Accession:
    A8K0Z3
    Secondary Accessions:

    Protein attributes for WASH1 Gene

    Size:
    465 amino acids
    Molecular mass:
    50328 Da
    Quaternary structure:
    • Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASH1, WASH2P or WASH3P), FAM21 (FAM21A or FAM21C), KIAA1033/SWIP, KIAA0196/strumpellin and CCDC53. The WASH core complex associates via FAM21 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex (PubMed:19922875, PubMed:20498093). Interacts (via WHD1 region) with FAM21C; the interaction is direct (PubMed:19922874). Interacts with VPS35; mediates the association with the retromer CSC complex. Interacts with FKBP15. Interacts with alpha-tubulin. Interacts with BECN1; WASH1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity). Interacts with EXOC1, EXOC4, EXOC8; in MMP14-positive endosomes in breast tumor cells; indicative for an association with the exocyst complex (PubMed:24344185).
    Miscellaneous:
    • WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (PubMed:18159949). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.

neXtProt entry for WASH1 Gene

Proteomics data for WASH1 Gene at MOPED

Post-translational modifications for WASH1 Gene

  • Ubiquitinated at Lys-220 via Lys-63-linked ubiquitin chains by the TRIM27:MAGEL2 E3 ubiquitin ligase complex, leading to promote endosomal F-actin assembly.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for WASH1 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for WASH1 Gene

Domains for WASH1 Gene

Gene Families for WASH1 Gene

HGNC:
  • WASH :WAS protein homologs

Protein Domains for WASH1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for WASH1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

A8K0Z3

UniProtKB/Swiss-Prot:

WASH1_HUMAN
Domain:
  • The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro.:
    • A8K0Z3
  • Contains 1 WH2 domain.:
    • A8K0Z3
Family:
  • Belongs to the WASH1 family.:
    • A8K0Z3
genes like me logo Genes that share domains with WASH1: view

Function for WASH1 Gene

Molecular function for WASH1 Gene

UniProtKB/Swiss-Prot Function: Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).

Gene Ontology (GO) - Molecular Function for WASH1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003779 actin binding IEA --
GO:0005515 protein binding IPI 19922874
GO:0031625 ubiquitin protein ligase binding IPI 23452853
GO:0043014 alpha-tubulin binding IDA 19922874
genes like me logo Genes that share ontologies with WASH1: view

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for WASH1 Gene

Localization for WASH1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for WASH1 Gene

Early endosome membrane. Recycling endosome membrane. Late endosome. Cytoplasmic vesicle, autophagosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Localization to the endosome membrane is mediated via its interaction with FAM21 (PubMed:19922874). Localizes to MMP14-positive late endosomes and transiently to invadipodia (PubMed:24344185). Localized to Salmonella typhimurium entry sites (By similarity). {ECO:0000250 UniProtKB:Q8VDD8, ECO:0000269 PubMed:19922874, ECO:0000269 PubMed:24344185}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for WASH1 Gene COMPARTMENTS Subcellular localization image for WASH1 gene
Compartment Confidence
endosome 5
cytoskeleton 2
cytosol 2
nucleus 2
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for WASH1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000145 colocalizes_with exocyst IDA 24344185
GO:0005769 early endosome ISS --
GO:0005770 late endosome IEA --
GO:0005776 autophagic vacuole IEA --
GO:0005814 centriole IEA --
genes like me logo Genes that share ontologies with WASH1: view

Pathways for WASH1 Gene

SuperPathways for WASH1 Gene

No Data Available

Interacting Proteins for WASH1 Gene

Gene Ontology (GO) - Biological Process for WASH1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006887 exocytosis IMP 24344185
GO:0015031 protein transport IEA --
GO:0016197 endosomal transport ISS --
GO:0022617 extracellular matrix disassembly IMP 24344185
GO:0030335 positive regulation of cell migration IMP 22114305
genes like me logo Genes that share ontologies with WASH1: view

No data available for Pathways by source for WASH1 Gene

Transcripts for WASH1 Gene

mRNA/cDNA for WASH1 Gene

(16) REFSEQ mRNAs :
(9) Additional mRNA sequences :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for WASH1 Gene

WAS protein family homolog 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for WASH1 Gene

No ASD Table

Relevant External Links for WASH1 Gene

GeneLoc Exon Structure for
WASH1
ECgene alternative splicing isoforms for
WASH1

Expression for WASH1 Gene

mRNA expression in normal human tissues for WASH1 Gene

mRNA expression in normal human tissues for WASH1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, MOPED, and MaxQB for WASH1 Gene

SOURCE GeneReport for Unigene cluster for WASH1 Gene Hs.461200

genes like me logo Genes that share expressions with WASH1: view

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for WASH1 Gene

Orthologs for WASH1 Gene

This gene was present in the common ancestor of animals.

Orthologs for WASH1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia WASH1 35
  • 98.21 (n)
  • 96.77 (a)
cow
(Bos Taurus)
Mammalia WASH1 35
  • 86.21 (n)
  • 88.58 (a)
dog
(Canis familiaris)
Mammalia WASH1 35
  • 87.24 (n)
  • 89.25 (a)
mouse
(Mus musculus)
Mammalia Wash 35
  • 80.75 (n)
  • 82.11 (a)
rat
(Rattus norvegicus)
Mammalia Wash 35
  • 80.1 (n)
  • 82.11 (a)
chicken
(Gallus gallus)
Aves WASH1 35
  • 72.9 (n)
  • 76.56 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia wash1 35
  • 67.26 (n)
  • 71.08 (a)
zebrafish
(Danio rerio)
Actinopterygii wash 35
  • 67.17 (n)
  • 66.59 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010756 35
  • 46.28 (n)
  • 31.83 (a)
fruit fly
(Drosophila melanogaster)
Insecta wash 35
  • 42.69 (n)
  • 33.58 (a)
Species with no ortholog for WASH1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for WASH1 Gene

ENSEMBL:
Gene Tree for WASH1 (if available)
TreeFam:
Gene Tree for WASH1 (if available)

Paralogs for WASH1 Gene

Selected SIMAP similar genes for WASH1 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with WASH1: view

No data available for Paralogs for WASH1 Gene

Variants for WASH1 Gene

Sequence variations from dbSNP and Humsavar for WASH1 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type MAF
rs3971592 -- 31,479(+) tgggc(A/G)acaga upstream-variant-2KB
rs9777085 -- 30,818(+) gaacc(C/T)gtcca upstream-variant-2KB
rs11490466 -- 15,933(+) CTGCT(G/T)CTTCT missense, reference
rs11555811 -- 18,481(-) CCAGC(A/G)GGTAG reference, missense
rs12354145 -- 19,971(+) GCTGT(C/T)TTAAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for WASH1 Gene

Variant ID Type Subtype PubMed ID
nsv8400 CNV Gain+Loss 18304495
nsv891939 CNV Loss 21882294
esv23366 CNV Gain+Loss 19812545
nsv471464 CNV Duplication 19718026
nsv482128 CNV Gain 20164927
esv1391017 CNV Deletion 17803354
esv1199599 CNV Deletion 17803354
esv989554 CNV Insertion 20482838

Relevant External Links for WASH1 Gene

HapMap Linkage Disequilibrium report
WASH1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for WASH1 Gene

Disorders for WASH1 Gene

(1) University of Copenhagen DISEASES for WASH1 Gene

genes like me logo Genes that share disorders with WASH1: view

No data available for UniProtKB/Swiss-Prot for WASH1 Gene

Publications for WASH1 Gene

  1. Human subtelomeric WASH genes encode a new subclass of the WASP family. (PMID: 18159949) Linardopoulou E.V. … Trask B.J. (PLoS Genet. 2007) 2 3 4
  2. A FAM21-containing WASH complex regulates retromer-dependent sorting. (PMID: 19922874) Gomez T.S. … Billadeau D.D. (Dev. Cell 2009) 3 4
  3. The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex. (PMID: 19922875) Derivery E. … Gautreau A. (Dev. Cell 2009) 3 4
  4. Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region. (PMID: 10655549) Ciccodicola A. … D'Urso M. (Hum. Mol. Genet. 2000) 3 4
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4

Products for WASH1 Gene

Sources for WASH1 Gene

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