V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

WASF3 Gene

protein-coding   GIFtS: 65

GC13P026029
WAS protein family, member 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
Brush-1 2
KIAA0900 1, 2, 3
OTTHUMP00000018160 2
SCAR3 1, 2, 3, 5
WAVE3 1, 2, 3, 5
Descriptions
Protein WAVE-3 3
Verprolin homology domain-containing protein 3 3
WAS protein family, member 3 2
WASP family Verprolin-homologous protein 3 2
WASP family protein member 3 3
External Ids
HGNC: 127341
Entrez Gene: 108102
UniProtKB: Q9UPY63
Ensembl: ENSG000001329707
Search outside databases for aliases for WASF3 gene

Previous GC identifers: GC13P025111 GC13P021111 GC13P026061 GC13P024929

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for WASF3:
This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a
protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin
occurs through a C-terminal verprolin homology domain in all family members. The multiprotein
complex serves to tranduce signals that involve changes in cell shape, motility or function.
[provided by RefSeq]

UniProtKB/Swiss-Prot: WASF3_HUMAN, Q9UPY6
Function: Downstream effector molecules involved in the transmission of signals from tyrosine
kinase receptors and small GTPases to the actin cytoskeleton

Gene Wiki entry for WASF3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WASF3 gene  

Entrez Gene cytogenetic band: 13q12   Ensembl cytogenetic band:  13q12.13   HGNC cytogenetic band: 13q12

WASF3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13P026029:     (about GC identifiers)

Start:
26,029,840 bp from pter
End:
26,161,082 bp from pter
Size:
131,243 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000013.9  NT_024524.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: WASF3_HUMAN, Q9UPY6 (See protein sequence)
Recommended Name: Wiskott-Aldrich syndrome protein family member 3  
Size: 502 amino acids; 55293 Da
Subunit: Binds actin and the Arp2/3 complex
Subcellular location: Cytoplasm, cytoskeleton
Secondary accessions: O94974

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006637.2  

    ENSEMBL proteins: 
    ENSP00000370877 ENSP00000335055 ENSP00000354325 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (WASF3)
    Human Recombinant Proteins from Abnova (WASF3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005856 cytoskeleton IEA--
    About this table

    Antibodies for WASF3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for WASF3
    R&D Systems Antibodies for WASF3 (WASF3/WAVE3)
    Cell Signaling Technology (CST) Antibodies for WASF3  (WAVE3)
    Antibodies from Abcam (WASF3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (WASF3)
    Novus Biologicals Antibodies for WASF3

    Assays for WASF3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR003124 WH2_actin_bd


       GeneDecks  WASF3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UPY6

    ProtoNet protein and cluster: Q9UPY6

    1 Blocks protein family: IPB003124 Actin-binding WH2

    UniProtKB/Swiss-Prot: WASF3_HUMAN, Q9UPY6
    Domain: Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology
    (VPH) domain
    Similarity: Belongs to the SCAR/WAVE family
    Similarity: Contains 1 WH2 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WASF3)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006646

                  Applied Biosystems Silencer® siRNAs for WASF3

                  Sigma-Aldrich siRNA for WASF3  
                         Sigma-Aldrich shRNA for WASF3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for WASF3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006646
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006646
                                     untagged cDNA clone in CMV expression vector: NM_006646 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006646

    UniProtKB/Swiss-Prot: WASF3_HUMAN, Q9UPY6
    Function: Downstream effector molecules involved in the transmission of signals from tyrosine
    kinase receptors and small GTPases to the actin cytoskeleton

    Genatlas biochemistry entry for WASF3:
    WAS protein family,member 3,widely expressed,strongly in brain,associating with the Arp2/3 complex
    a nucleating core for actin polymerization in vitro

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779 actin binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    2 Invitrogen iPath™ Online BioAtlas - Pathways for WASF3 (Maps provided by GeneGo):
     CDC42 in cellular processes
     Focal adhesion complex formation: WASF-proteins and activation of RAP GTP-ase

       GeneDecks  WASF3 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for WASF3):
     hsa04520 Adherens junction

       GeneDecks  WASF3 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  WASF3 


    5/12 Interacting proteins for WASF3 (ENSP000003350553) via UniProtKB, MINT, and/or STRING (see all 12 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BAIAP2ENSP000003163383STRING (score=.967)
    ACTBENSP000003499603STRING (score=.943)
    WASLENSP000002230233STRING (score=.922)
    WASENSP000003658913STRING (score=.896)
    ACTG1ENSP000003315143STRING (score=.873)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006461 protein complex assembly TAS10381382
    GO:0030041 actin filament polymerization TAS10381382
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for WASF3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WASF3)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006646

                  Sigma-Aldrich siRNA for WASF3  
                         Sigma-Aldrich shRNA for WASF3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    REFSEQ mRNAs for WASF3 gene: 

    NM_006646.5   


                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006646
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006646
                                     untagged cDNA clone in CMV expression vector: NM_006646 

    Additional cDNA sequence: 

    AB020707.2 AB026543.1 AF134305.1 AF454702.1 AK289845.1 AK294725.1 AL133590.1 BC050283.1 
    S69790.1 

    6 DOTS entries:

    DT.216021  DT.120780437  DT.120780442  DT.40224092  DT.91917303  DT.95077878 

    24/118 AceView cDNA sequences (see all 118 ):

    NM_006646 Z39727 BM671339 BM661863 BM462444 H39517 BQ446736 BM703028 
    BM693418 AI424535 AI127514 F11179 BU736040 Z44600 AA629542 T08945 
    BU729251 BM668695 BU176547 AF134305 AB020707 N34667 AW771824 BG773052 

    highest scoring ESTs for WASF3:

    S69790 AA063205 AA080918 AA084352 AA164760 AA393357 AA398689 AA625453 AA629542 AA701002 

    Unigene Cluster for WASF3:

    WAS protein family, member 3
    Hs.635221  [show with all ESTs]
    Unigene Representative Sequence: NM_006646


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000381468  ENST00000335327  ENST00000361042  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    WASF3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for WASF3

    1 / 2 / 3

    3 probe-sets matching WASF3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    1058_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 S69790 1.00 1.00 1.00 1

    204042_at2, 3 U133-A 1 1.00 1.00 -- -- AB020707 0.80 1.00 0.91 1

    204042_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  WASF3 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATACATATTA

    SOURCE GeneReport for Unigene cluster: Hs.635221

    Expression variation in blood from EXPOLDB for WASF3

    UniProtKB/Swiss-Prot: WASF3_HUMAN, Q9UPY6
    Tissue specificity: Expressed in ovary and brain

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for WASF3 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    WASF31   -- WAS protein family, member 3 89.97(n)
    96.22(a)
    477331  XM_534525.2  XP_534525.2 
    chimpanzee
    (Pan troglodytes)
    WASF31   -- WAS protein family, member 3 93.7(n)
    91.75(a)
    452502  XM_001157344.1  XP_001157344.1 
    cow
    (Bos taurus)
    WASF31   -- WAS protein family, member 3 86.09(n)
    94.01(a)
    540674  XM_865488.2  XP_870581.1 
    mouse
    (Mus musculus)
    Wasf31, 5 55
    WAS protein family, member 31, 5 86.03(n)1
    93.81(a)1
    2458801  NM_145155.21  NP_660137.11 
     AF4547035  AI8540465  (see all 8)
    chicken
    (Gallus gallus)
    WASF31   -- WAS protein family, member 3 79.31(n)
    87.03(a)
    418934  XM_417128.2  XP_417128.2 
    About this table        Species with no ortholog for WASF3

    ENSEMBL Gene Tree for WASF3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for WASF3 gene
    WASF42  WASF22  WASF12  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/877 NCBI SNPs in WASF3 are shown (see all 877 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 298)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 13 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs19275161,2
    A,C,F,H,O26158589(-) ATATTT/CGACAG 1 -- ut3111Minor allele frequency- C:0.21NA EU EA WA 974
    rs5420661,2
    A,C,F,H,O26028462(+) CTAAAC/TATAGA 1 -- ng5112Minor allele frequency- N:0.00EU EA WA NA 1048
    rs4812201,2
    A,C,F,H26029032(-) CATCTA/CATAGA 1 -- ng514Minor allele frequency- C:0.39EU EA WA 412
    rs47695041,2
    A,C,F,H26028516(+) AAGTCA/GTTTTA 1 -- ng517Minor allele frequency- G:0.39NA EU EA WA 524
    rs20254131,2
    A,C,F,O26158914(-) GCTCAC/TTCAGG 1 -- ut31 ese33Minor allele frequency- T:0.41NA 138
    rs175483961,2
    C,F,H26158831(+) TACACC/TGCTGA 1 -- ut31 ese37Minor allele frequency- T:0.11NA EU EA WA 544
    rs95538541,2
    A,C,H26029270(+) GGTTAC/TAGGGT 1 -- ng514Minor allele frequency- T:0.00EU EA WA 396
    rs95123321,2
    A,F,H26161549(+) CATGTT/GGGGGT 1 -- ng314Minor allele frequency- G:0.30EU EA WA 420
    rs105073701,2
    C,F,H26159373(+) TAAGAC/TGCTTG 1 -- ut3112Minor allele frequency- T:0.01NS NA EU EA WA 664
    rs101591,2
    C,F,H26160949(+) TGTTGT/AAAATT 1 -- ut318Minor allele frequency- A:0.01MN EU EA WA NA 651
    About this table

    HapMap Linkage Disequilibrium images for WASF3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605068

    2 Novoseek disease relationships for WASF3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    ganglioneuroblastoma 81.80 2 12185600 (1), 12856283 (1)
    wiskott-aldrich syndrome 72.66 1 16417486 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/22 PubMed articles for WASF3 gene (see all 22 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10810 HGNC: 12734 AceView: WASF3 Ensembl:ENSG00000132970 euGenes: HUgn10810
    ECgene: WASF3 H-InvDB: WASF3
    (According to HUGE)
    About This Section

    HUGE: KIAA0900
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for WASF3 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for WASF3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for WASF3-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for WASF3 Antibodies for WASF3
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for WASF3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (WASF3)
     Antibodies (WASF3)
     Search for Chimera RNAi
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Antibodies & Assays for WASF3  (WAVE3)

     Recombinant Proteins
    (WASF3)
     Antibodies (WASF3)
     Search OriGene for WASF3
     Search Tocris compounds for WASF3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for WASF3

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    WASF3 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com