Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



WASF1 Gene

protein-coding   GIFtS: 64
GCID: GC06M110421

WAS Protein Family, Member 1

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
WAS Protein Family, Member 11 2     WASP Family Protein Member 12 3
SCAR12 3 5     WAVE2 5
WAVE12 3 5     Homology Of Dictyostelium Scar 12
Protein WAVE-12 3     Wiskott-Aldrich Syndrome Protein Family Member 12
Verprolin Homology Domain-Containing Protein 12 3     KIAA02693

External Ids:    HGNC: 127321   Entrez Gene: 89362   Ensembl: ENSG000001122907   OMIM: 6050355   UniProtKB: Q925583   

Export aliases for WASF1 gene to outside databases

Previous GC identifers: GC06M110256 GC06M110443 GC06M110466 GC06M110527 GC06M107987


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for WASF1 Gene:
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical
role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane
ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin
polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in
regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein
have been found for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for WASF1 Gene:
WASF1 (WAS protein family, member 1) is a protein-coding gene. Diseases associated with WASF1 include wiskott-aldrich syndrome, and shigellosis. GO annotations related to this gene include actin binding. An important paralog of this gene is WASF2.

UniProtKB/Swiss-Prot: WASF1_HUMAN, Q92558
Function: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and
small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that
regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction
with the Arp2/3 complex

Gene Wiki entry for WASF1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000006.12  NT_025741.16  NC_018917.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the WASF1 gene promoter:
         HFH-3   AML1a   Nkx2-5   Egr-4   Evi-1   FOXI1   AREB6   S8   FOXO1a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidWASF1 promoter sequence
   Search Chromatin IP Primers for WASF1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WASF1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q21   Ensembl cytogenetic band:  6q21   HGNC cytogenetic band: 6q21

WASF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WASF1 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M110421:  view genomic region     (about GC identifiers)

Start:
110,421,022 bp from pter      End:
110,501,207 bp from pter
Size:
80,186 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: WASF1_HUMAN, Q92558 (See protein sequence)
Recommended Name: Wiskott-Aldrich syndrome protein family member 1  
Size: 559 amino acids; 61652 Da
Subunit: Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within
the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and
BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The
complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with
SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via
N-terminus)
Sequence caution: Sequence=BAA13399.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
1 PDB 3D structure from and Proteopedia for WASF1:
3P8C (3D)    
Secondary accessions: E1P5F2 Q5SZK7

Explore the universe of human proteins at neXtProt for WASF1: NX_Q92558

Explore proteomics data for WASF1 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See WASF1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001020105.1  NP_001020106.1  NP_001020107.1  NP_003922.1  

    ENSEMBL proteins: 
     ENSP00000376366   ENSP00000376365   ENSP00000407041   ENSP00000265601   ENSP00000357934  
     ENSP00000402663   ENSP00000404142   ENSP00000376368   ENSP00000376367   ENSP00000352425  
    Reactome Protein details: Q92558

    WASF1 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for WASF1
    OriGene Custom MassSpec
    OriGene Custom Protein Services for WASF1
    GenScript Custom Purified and Recombinant Proteins Services for WASF1
    Novus Biologicals WASF1 Protein
    Novus Biologicals WASF1 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for WASF1

    WASF1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of WASF1
    R&D Systems Antibodies for WASF1 (WASF1/WAVE1)
    OriGene Antibodies for WASF1
    OriGene Custom Antibody Services for WASF1
    Novus Biologicals WASF1 Antibodies
    Abcam antibodies for WASF1
    Cloud-Clone Corp. Antibodies for WASF1
    Search ThermoFisher Antibodies for WASF1
    LSBio Antibodies in human, mouse, rat for WASF1

    WASF1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for WASF1
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for WASF1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for WASF1
    Cloud-Clone Corp. CLIAs for WASF1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    AKAP: A-kinase anchor proteins

    2 InterPro protein domains:
     IPR028288 SCAR/WAVE_fam
     IPR003124 WH2_dom

    Graphical View of Domain Structure for InterPro Entry Q92558

    ProtoNet protein and cluster: Q92558

    1 Blocks protein domain: IPB003124 Actin-binding WH2

    UniProtKB/Swiss-Prot: WASF1_HUMAN, Q92558
    Domain: Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain
    Similarity: Belongs to the SCAR/WAVE family
    Similarity: Contains 1 WH2 domain


    WASF1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: WASF1_HUMAN, Q92558
    Function: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and
    small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that
    regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction
    with the Arp2/3 complex

         Genatlas biochemistry entry for WASF1:
    WAS protein family,member1,widely expressed,strongly in brain,associating with the Arp2/3 complex a nucleating
    core for actin polymerization in vitro,potentially involved in the regulation of RAC induced of actin
    cytoskeleton required for membrane ruffling

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779actin binding IEA--
    GO:0005515protein binding IPI9843499
    GO:0032403protein complex binding IMP--
    GO:0048365contributes to Rac GTPase binding IMP--
         
    WASF1 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for WASF1:
     Increased G1 DNA content 

         7 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Wasf1):
     behavior/neurological  digestive/alimentary  growth/size/body  liver/biliary system  mortality/aging 
     muscle  nervous system 

    WASF1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Wasf1tm1Jsco for WASF1

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for WASF1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for WASF1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for WASF1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for WASF1

    miRNA
    Products:
        
    miRTarBase miRNAs that target WASF1:
    hsa-let-7b-5p (MIRT052008), hsa-mir-122-5p (MIRT023340)

    Block miRNA regulation of human, mouse, rat WASF1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate WASF1 (see all 29):
    hsa-miR-140-5p hsa-miR-607 hsa-miR-34b* hsa-miR-429 hsa-miR-4328 hsa-miR-29a hsa-miR-29c hsa-miR-449a
    SwitchGear 3'UTR luciferase reporter plasmidWASF1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for WASF1
    Predesigned siRNA for gene silencing in human, mouse, rat WASF1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for WASF1

    Clone
    Products:
         
    OriGene clones in human, mouse for WASF1 (see all 21)
    OriGene ORF clones in mouse, rat for WASF1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): WASF1 (NM_001024935)
    Sino Biological Human cDNA Clone for WASF1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WASF1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WASF1

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for WASF1
    Browse ESI BIO Cell Lines and PureStem Progenitors for WASF1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WASF1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    WASF1_HUMAN, Q92558: Cytoplasm, cytoskeleton. Cell junction, synapse (By similarity). Note=Dot-like pattern in
    the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    cytosol2
    mitochondrion2
    nucleus2
    endosome1

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ----
    GO:0005739mitochondrion ----
    GO:0005741mitochondrial outer membrane IEA--
    GO:0005856cytoskeleton TAS10970852
    GO:0015629actin cytoskeleton TAS9843499

    WASF1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for WASF1 About   (see all 18)  
    See pathways by source

    SuperPathContained pathways About
    1Regulation of actin cytoskeleton
    Regulation of actin cytoskeleton0.57
    Regulation of Actin Cytoskeleton0.57
    2Fcgamma receptor (FCGR) dependent phagocytosis
    Regulation of actin dynamics for phagocytic cup formation0.77
    Fcgamma receptor (FCGR) dependent phagocytosis0.77
    3Actin Nucleation by ARP-WASP Complex
    Actin Nucleation by ARP-WASP Complex0.66
    RhoA Pathway0.35
    4Apoptotic Pathways in Synovial Fibroblasts
    Rac1 Pathway0.65
    Actin-Based Motility by Rho Family GTPases0.62
    5Salmonella infection (KEGG)
    Shigellosis0.30
    Salmonella infection0.30

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for WASF1 (see all 9)
        RhoA Pathway
    Actin-Based Motility by Rho Family GTPases
    Internalin Pathway
    Transendothelial Migration of Leukocytes
    Actin Nucleation by ARP-WASP Complex

    3 GeneGo (Thomson Reuters) Pathways for WASF1
        Signal transduction PKA signaling
    Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases
    G-protein signaling RAC1 in cellular process

    3 BioSystems Pathways for WASF1
        Regulation of Actin Cytoskeleton
    E2F transcription factor network
    RAC1 signaling pathway


    1 Reactome Pathway for WASF1
        Regulation of actin dynamics for phagocytic cup formation


    Selected Kegg Pathways  (Kegg details for WASF1) (see all 6):
        Adherens junction
    Fc gamma R-mediated phagocytosis
    Regulation of actin cytoskeleton
    Bacterial invasion of epithelial cells
    Shigellosis


    WASF1 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including WASF1: 
              Cytoskeleton Regulators in human mouse rat
              Adherens Junctions in human mouse rat
              Cell Motility in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for WASF1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for WASF1 (Q925581, 2, 3 ENSP000003524254) via UniProtKB, MINT, STRING, and/or I2D (see all 80)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABL1P005192, 3, ENSP000003614234MINT-16419 MINT-16418 MINT-16420 I2D: score=3 STRING: ENSP00000361423
    PFN1P077371, 3, ENSP000002256554EBI-1548747,EBI-713780 I2D: score=3 STRING: ENSP00000225655
    BRK1Q8WUW12, 3MINT-17058 I2D: score=2 
    NCKAP1Q9Y2A72, 3, ENSP000003542514MINT-8307391 MINT-15828 I2D: score=3 STRING: ENSP00000354251
    CYFIP2Q96F072, 3, ENSP000003909484MINT-14901 I2D: score=3 STRING: ENSP00000390948
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006461protein complex assembly NAS10381382
    GO:0006928cellular component movement TAS2775266
    GO:0016601Rac protein signal transduction IMP--
    GO:0030036actin cytoskeleton organization ----
    GO:0030041actin filament polymerization TAS9843499

    WASF1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for WASF1



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for WASF1 gene (4 alternative transcripts): 
    NM_001024934.1  NM_001024935.1  NM_001024936.1  NM_003931.2  

    Unigene Cluster for WASF1:

    WAS protein family, member 1
    Hs.75850  [show with all ESTs]
    Unigene Representative Sequence: NM_003931
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000392587 ENST00000392586 ENST00000444391 ENST00000265601 ENST00000368938
    ENST00000447287 ENST00000419252 ENST00000392589(uc003ptv.1) ENST00000392588(uc003ptw.1)
    ENST00000359451(uc003ptx.1 uc003pty.1 uc003ptz.1)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat WASF1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate WASF1 (see all 29):
    hsa-miR-140-5p hsa-miR-607 hsa-miR-34b* hsa-miR-429 hsa-miR-4328 hsa-miR-29a hsa-miR-29c hsa-miR-449a
    SwitchGear 3'UTR luciferase reporter plasmidWASF1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for WASF1
    Predesigned siRNA for gene silencing in human, mouse, rat WASF1
    Clone
    Products:
         
    OriGene clones in human, mouse for WASF1 (see all 21)
    OriGene ORF clones in mouse, rat for WASF1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): WASF1 (NM_001024935)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WASF1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WASF1
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for WASF1
    OriGene qSTAR qPCR primer pairs in human, mouse for WASF1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WASF1
      QuantiTect SYBR Green Assays in human, mouse, rat WASF1
      QuantiFast Probe-based Assays in human, mouse, rat WASF1

    Additional mRNA sequence: 

    AF134303.1 AK097838.1 BC044591.1 BC068546.1 D87459.1 

    20 DOTS entries:

    DT.97786672  DT.65284123  DT.95178572  DT.100736715  DT.95359203  DT.451630  DT.40116047  DT.100730920 
    DT.100792169  DT.121322679  DT.100047436  DT.121322706  DT.121322771  DT.121322718  DT.97781907  DT.100658902 
    DT.121322689  DT.121322726  DT.121322836  DT.75173015 

    Selected AceView cDNA sequences (see all 122):

    BM458302 AF134303 AI306688 CR622893 AK097838 F10585 AU142666 CB215686 
    BI461486 D87459 CD171651 BX477915 NM_003931 AW590436 BC044591 BX425187 
    AI928895 CR601979 AW612159 AA349197 BE787088 BG492540 BI462401 D57242 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for WASF1    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12 ^ 13a · 13b
    SP1:              -                                               -     -           -                           
    SP2:              -     -                                                           -                           
    SP3:                    -                                                           -                           
    SP4:                                                                                                            


    ECgene alternative splicing isoforms for WASF1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    WASF1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TAACTTACAT
    WASF1 Expression
    About this image


    WASF1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 6) fully expand
     
     Brain (Nervous System)    fully expand to see all 3 entries
             Cerebellum
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Ovary (Reproductive System)
             Oviduct
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    WASF1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    WASF1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.75850

    UniProtKB/Swiss-Prot: WASF1_HUMAN, Q92558
    Tissue specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus,
    small intestine and peripheral blood

        Pathway & Disease-focused RT2 Profiler PCR Arrays including WASF1: 
              Cytoskeleton Regulators in human mouse rat
              Adherens Junctions in human mouse rat
              Cell Motility in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for WASF1
    OriGene qSTAR qPCR primer pairs in human, mouse for WASF1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WASF1
    QuantiTect SYBR Green Assays in human, mouse, rat WASF1
    QuantiFast Probe-based Assays in human, mouse, rat WASF1
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WASF1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for WASF1 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Wasf11 , 5 WASP family 15
    WAS protein family, member 11
    91(n)1
    98.39(a)1
      10 (22.07 cM)5
    837671  NM_031877.31  NP_114083.11 
     408834755 
    chicken
    (Gallus gallus)
    Aves WASF11 WAS protein family, member 1 82.83(n)
    94.63(a)
      421761  XM_004940308.1  XP_004940365.1 
    lizard
    (Anolis carolinensis)
    Reptilia WASF16
    WAS protein family, member 1
    91(a)
    1 ↔ 1
    1(191461288-191492470)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.241262 Xenopus laevis transcribed sequence with moderate similarity more 74.16(n)    BU901885.1 
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkeyp-88f5.51 si:dkeyp-88f5.5 72.9(n)
    91.81(a)
      100001221  XM_001335766.5  XP_001335802.5 
    fruit fly
    (Drosophila melanogaster)
    Insecta SCAR3 actin binding 48(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea wve-16
    Protein WVE-1, isoform a
    31(a)
    1 → many
    I(11924785-11929507) WBGene00006958


    ENSEMBL Gene Tree for WASF1 (if available)
    TreeFam Gene Tree for WASF1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for WASF1 gene
    WASF22  WASF32  
    2 SIMAP similar genes for WASF1 using alignment to 4 protein entries:     WASF1_HUMAN (see all proteins):
    WASF2    WASF3

    WASF1 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for WASF1
    PGOHUM00000240837


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for WASF1 (see all 1393)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1132739341,2
    F--110420610(+) TTTTTG/ATAGAG 4 -- ds50011Minor allele frequency- A:0.50CSA 2
    rs1839956661,2
    --110420818(+) CCACAA/GGCAGT 4 -- ds50010--------
    rs1120091191,2
    F--110420920(+) ATTATG/ATAGCC 4 -- ds50011Minor allele frequency- A:0.50CSA 2
    rs1500493891,2
    --110420986(+) AAATTA/GTGATA 4 -- ds50010--------
    rs1454120941,2
    --110420994(+) ATAAAC/TCATTT 4 -- ds50010--------
    rs1410881441,2
    --110421073(+) AGTTAG/TGTAAG 4 -- ut310--------
    rs1469367901,2
    C--110421128(+) TAACTC/TCTATA 4 -- ut310--------
    rs1452104381,2
    C--110421230(+) TAATG-/AAAAAA 4 -- ut310--------
    rs1379371451,2
    --110421277(+) CTAAAC/TGTACT 4 -- ut310--------
    rs1153223341,2
    C,F--110421286(+) CTTTCT/CGAGTG 4 -- ut311Minor allele frequency- C:0.03WA 118

    HapMap Linkage Disequilibrium report for WASF1 (110421022 - 110501207 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 9 variations for WASF1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2676234CNV Deletion23128226
    esv2732567CNV Deletion23290073
    nsv436521CNV Deletion17901297
    esv267753CNV Insertion20981092
    esv33528CNV Loss17666407
    nsv886536CNV Loss21882294
    nsv348708CNV Loss16902084
    nsv886535CNV Loss21882294
    dgv2001e1CNV Complex17122850

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing WASF1
    DNA2.0 Custom Variant and Variant Library Synthesis for WASF1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 605035    OMIM disorders: --

    15 diseases for WASF1:    
    About MalaCards
    wiskott-aldrich syndrome    shigellosis    acute lymphocytic leukemia    gout
    mental retardation    multiple sclerosis    ovarian cancer    tuberculosis
    alzheimer's disease    leukemia    pancreatitis    endotheliitis
    cerebritis    neuronitis    breast cancer


    WASF1 for disorders           About GeneDecksing

    4 Novoseek inferred disease relationships for WASF1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    scar1 94.6 34 9889097 (8), 12601690 (6), 11747816 (6), 12429845 (4) (see all 5)
    wiskott-aldrich syndrome 91.9 16 12601690 (1), 12853106 (1), 18287015 (1), 9889097 (1) (see all 16)
    scar 54.5 5 11282031 (1), 17314411 (1), 14765121 (1), 12482910 (1) (see all 5)
    mental retardation 15.5 2 12578964 (1), 17215396 (1)

    Human Genome Epidemiology (HuGE) Navigator: WASF1 (1 document)

    Export disorders for WASF1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for WASF1 gene, integrated from 10 sources (see all 97):
    (articles sorted by number of sources associating them with WASF1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. (PubMed id 9889097)1, 2, 3, 9 Machesky L.M. and Insall R.H. (Curr. Biol. 1998)
    2. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. (PubMed id 9843499)1, 3, 9 Miki H....Takenawa T. (EMBO J. 1998)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. (PubMed id 11130076)1, 2 Miki H.... Takenawa T. (Nature 2000)
    5. SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development. (PubMed id 9732292)1, 2 Bear J.E.... Saxe C.L. III (J. Cell Biol. 1998)
    6. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. (PubMed id 9039502)1, 2 Nagase T.... Nomura N. (DNA Res. 1996)
    7. [Interaction of WAVE1 and genes involved in multiple drug resistance in children with acute myeloblastic leukemia]. (PubMed id 20426950)1, 9 Yang M.H....Cao L.Z. (Zhonghua Er Ke Za Zhi 2010)
    8. [Expression of WAVE1 in childhood acute lymphocytic leukemia and in the apoptosis of Jurkat cells induced by adriamycin]. (PubMed id 18947485)1, 9 Wang Z....Xu W.Q. (Zhongguo Dang Dai Er Ke Za Zhi 2008)
    9. [Role of WAVE1 in K562 leukemia cells invasion and its mechanism]. (PubMed id 19731823)1, 9 He Y.L....Xu W.Q. (Zhonghua Xue Ye Xue Za Zhi 2009)
    10. Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex. (PubMed id 10381382)1, 9 Suetsugu S.... Takenawa T. (Biochem. Biophys. Res. Commun. 1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 8936 HGNC: 12732 AceView: WASF1 Ensembl:ENSG00000112290 euGenes: HUgn8936
    ECgene: WASF1 Kegg: 8936 H-InvDB: WASF1

    (According to HUGE)
    About This Section

    TryGeneCards Plus
    HUGE: KIAA0269

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for WASF1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for WASF1 gene:
    Search GeneIP for patents involving WASF1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Kits and Assays available from EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Small Molecules at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of WASF1
     
     EMD Millipore genomic analysis products

      
     Antibodies for WASF1 (WASF1/WAVE1)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for WASF1   OriGene RNAi products in human, mouse, rat for WASF1  
     OriGene qPCR primer pairs and template standards for WASF1   OriGene Protein Over-expression Lysate for WASF1  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for WASF1  
     OriGene qSTAR qPCR primer pairs in human, mouse for WASF1   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for WASF1   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for WASF1   OriGene Custom Protein Services for WASF1  

     
     
     Block miRNA regulation of human, mouse, rat WASF1 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing WASF1
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WASF1 Predesigned siRNA for gene silencing in human, mouse, rat WASF1
     QuantiFast Probe-based Assays in human, mouse, rat WASF1 QuantiTect SYBR Green Assays in human, mouse, rat WASF1
     PCR Arrays including human, mouse, rat WASF1 Search Chromatin IP Primers for WASF1
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WASF1  GeneGlobe Interaction Network for WASF1
     Regulatory tfbs in WASF1 promoter
     GenScript Custom Purified and Recombinant Proteins Services for WASF1 GenScript cDNA clones with any tag delivered in your preferred vector for WASF1
     GenScript Custom Assay Services for WASF1 GenScript Custom overexpressing Cell Line Services for WASF1
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for WASF1
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for WASF1
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     WASF1 antibodies
     WASF1 proteins
     WASF1 lysates
     Antibodies for WASF1
     See all of Abcam's Antibodies, Kits and Proteins for WASF1
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for WASF1
     Antibodies for WASF1
     ELISAs for WASF1
     CLIAs for WASF1



     Browse ESI BIO Cell Lines and PureStem Progenitors for WASF1
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WASF1
     SwitchGear 3'UTR luciferase reporter plasmids for WASF1
     SwitchGear Promoter luciferase reporter plasmids for WASF1
     Search ThermoFisher Antibodies for WASF1
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WASF1
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for WASF1
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for WASF1
     LSBio Antibodies in human, mouse, rat for WASF1
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      WASF1 gene at Home site.
    Version: 3.12.162 20 Aug 2014
    hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4