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WARS2 Gene

protein-coding   GIFtS: 59

GC01M119286
tryptophanyl tRNA synthetase 2, mitochondrial
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
(Mt)TrpRS 3
EC 6.1.1.2 3
OTTHUMP00000014272 2
OTTHUMP00000014273 2
TrpRS 1, 2, 3
Descriptions
Tryptophan--tRNA ligase 3
mitochondrial tryptophanyl tRNA synthetase 2 2
tryptophan tRNA ligase 2, mitochondrial 2
tryptophan-tRNA ligase 2
tryptophanyl tRNA synthetase 2, mitochondrial 2
External Ids
HGNC: 127301
Entrez Gene: 103522
UniProtKB: Q9UGM63
Ensembl: ENSG000001168747
Search outside databases for aliases for WARS2 gene

Previous GC identifers: GC01M120022 GC01M118413 GC01M118720 GC01M118920

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for WARS2:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because
of their central role in linking amino acids with nucleotide triplets contained in tRNAs,
aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution.
Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a
mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase.
Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Gene Wiki entry for WARS2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WARS2 gene  

Entrez Gene cytogenetic band: 1p13.3-p13.1   Ensembl cytogenetic band:  1p12   HGNC cytogenetic band: 1p12

WARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M119286:     (about GC identifiers)

Start:
119,375,362 bp from pter
End:
119,484,818 bp from pter
Size:
109,457 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_019273.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYWM_HUMAN, Q9UGM6 (See protein sequence)
Recommended Name: Tryptophanyl-tRNA synthetase, mitochondrial precursor  
Size: 360 amino acids; 40147 Da
Subcellular location: Mitochondrion matrix
Secondary accessions: Q53FT4 Q5VUD2 Q86TQ0

REFSEQ proteins (2 alternative transcripts): 
NP_056651.1  NP_957715.1  


ENSEMBL proteins: 
ENSP00000235521 ENSP00000358434 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (WARS2)
Human Recombinant Proteins from Abnova (WARS2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for WARS2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for WARS2
Browse R&D Systems for Antibodies
Antibodies from Abcam (WARS2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (WARS2)
Novus Biologicals Antibodies for WARS2

Assays for WARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR002305 aa-tRNA-synth_Ib
 IPR001412 aa-tRNA-synth_I_CS
 IPR014729 Rossmann-like_a/b/a_fold
 IPR002306 Trp-tRNA-synth_Ib


   GeneDecks  WARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9UGM6

ProtoNet protein and cluster: Q9UGM6

1 Blocks protein family: IPB002305 Aminoacyl-tRNA synthetase

UniProtKB/Swiss-Prot: SYWM_HUMAN, Q9UGM6
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (WARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_201263

              Applied Biosystems Silencer® siRNAs for WARS2

              Sigma-Aldrich siRNA and siRNA Panels for WARS2  
                     Sigma-Aldrich shRNA for WARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for WARS2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_015836
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_015836
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_015836 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_015836

UniProtKB/Swiss-Prot: SYWM_HUMAN, Q9UGM6
Catalytic activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Enzyme Number (IUBMB): EC 6.1.1.2 

Genatlas biochemistry entry for WARS2:
tryptophanyl-tRNA synthetase 2,mitochondrial

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0004830 tryptophan-tRNA ligase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for WARS2 (Maps provided by GeneGo):
 ATP metabolism

   GeneDecks  WARS2 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  WARS2  (Your Favorite Gene powered by Ingenuity) 
 Aminoacyl-tRNA Biosynthesis
 Tryptophan Metabolism

   GeneDecks  WARS2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for WARS2):
 hsa00380 Tryptophan metabolism
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  WARS2 for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  WARS2 


5/11 Interacting proteins for WARS2 (ENSP000002355213) via UniProtKB, MINT, and/or STRING (see all 11 )
InteractantInteraction Details
GeneCardExternal ID(s)
WARSENSP000003474953STRING (score=.976)
TDO2ENSP000002815253STRING (score=.937)
DDCENSP000003399283STRING (score=.899)
IDO1ENSP000002535133STRING (score=.899)
TPH1ENSP000003435503STRING (score=.899)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006436 tryptophanyl-tRNA aminoacylation IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for WARS2
4 Novoseek chemical compound relationships for WARS2 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
trna(trp) 85.97 3 11600701 (2), 7743129 (1)
aminoacyl-trna 77.25 3 7743129 (1), 8643392 (1), 12438642 (1)
trna 58.41 4 7743129 (1), 8643392 (1), 17637340 (1)
atp 6.14 1 7743129 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (WARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_201263

              Sigma-Aldrich siRNA and siRNA Panels for WARS2  
                     Sigma-Aldrich shRNA for WARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_015836  NM_201263  

REFSEQ mRNAs for WARS2 gene (2 alternative transcripts): 

NM_015836.3   NM_201263.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_015836  NM_201263  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_015836
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_015836
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_015836 

Additional cDNA sequence: 

AJ242739.1 AK056365.1 AK223197.1 AK296088.1 AK296799.1 AK299547.1 AK300295.1 AK313740.1 
BC021722.1 BC037821.2 BC039889.1 BC044575.1 CR590727.1 CR599172.1 CR621696.1 

10 DOTS entries:

DT.97840154  DT.95270607  DT.313244  DT.444801  DT.91854676  DT.91744934  DT.97840153  DT.100813783 
DT.121449946  DT.101956725 

24/137 AceView cDNA sequences (see all 137 ):

BM985401 BE275735 BQ014588 NM_015836 BQ432000 BM834499 BC039889 BX355578 
BU839073 AA194238 Z40749 BM802067 AW195495 BU181461 AL519147 CR590727 
BP336381 CR621696 CR599172 BE394734 AW068958 NM_201263 BX355579 BF447233 

highest scoring ESTs for WARS2:

AA227572 AA251468 AA332220 AJ242739 AL709389 BC021722 BC039889 BC044575 BE274756 BE275735 

Unigene Cluster for WARS2:

Tryptophanyl tRNA synthetase 2, mitochondrial
Hs.523506  [show with all ESTs]
Unigene Representative Sequence: BC039889


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for WARS2

ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7a · 7b
SP1:                    -                                             
SP2:                    -           -           -                     
SP3:                                                                  

About this scheme

ECgene alternative splicing isoforms for WARS2

2 Ensembl transcripts including schematic representations:
ENST00000235521  ENST00000369426  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

WARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for WARS2

1 / 2 / 3

8 probe-sets matching WARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
49539_at2, 3 U95-B 1 1.00 1.00 0.88 1.03 R40635 0.60 1.00 0.82 1

62728_at2, 3 U95-C 1 1.00 1.00 0.71 1.16 AI984673 0.60 1.00 0.82 1
62729_g_at2, 3 U95-C 1 1.00 1.00 0.87 1.15 AI984673 0.60 1.00 0.82 1

65548_at2, 3 U95-C 1 0.94 1.00 0.74 0.67 AI927965 0.60 1.00 0.82 1

218766_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_015836 0.60 1.00 0.82 1

222734_at2, 3 U133-B 1 1.00 1.00 -- -- BF515963 0.40 1.00 0.76 1

218766_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222734_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  WARS2 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GGTAAAGGAA

SOURCE GeneReport for Unigene cluster: Hs.523506
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for WARS2 gene from 5/19 species (see all 19 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
WARS21   -- tryptophanyl tRNA synthetase 2, mitochondrial 84.64(n)
87.65(a)
483145  XM_540263.2  XP_540263.2 
chimpanzee
(Pan troglodytes)
WARS21   -- tryptophanyl tRNA synthetase 2, mitochondrial 99.44(n)
100(a)
457162  XM_513688.2  XP_513688.1 
cow
(Bos taurus)
WARS21   -- tryptophanyl tRNA synthetase 2, mitochondrial 88.43(n)
89.44(a)
532903  NM_001034582.1  NP_001029754.1 
rat
(Rattus norvegicus)
Wars21   -- tryptophanyl tRNA synthetase 2 (mitochondrial) 84.63(n)
84.17(a)
690654  XM_001075111.1  XP_001075111.1 
mouse
(Mus musculus)
Wars21, 5 35
tryptophanyl tRNA synthetase 2 (mitochondrial)1, 5 85.28(n)1
86.39(a)1
705601  NM_027462.31  NP_081738.21 
 AI4133755  AK0176025  (see all 15)
About this table        Species with no ortholog for WARS2

ENSEMBL Gene Tree for WARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/523 NCBI SNPs in WARS2 are shown (see all 523 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 192)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs26452941,2
A,C,F,H,O119376110(+) TCCAAT/CACCCA 2 -- ut3111Minor allele frequency- C:0.44NA EU EA WA 976
rs45080581,2
A,C,F,H119485773(+) CAGTTT/CTTCTG 2 -- ng314Minor allele frequency- C:0.20EU EA WA 420
rs10579911,2
A,C,F,H119376693(-) CACACG/TTTATC 2 -- ut31 ese38Minor allele frequency- T:0.25MN NA EU EA WA 744
rs89281,2
A,C,F,H119375597(-) ACGCCG/CCATCC 2 -- ut31 ese38Minor allele frequency- C:0.30MN NA EU EA WA 966
rs10579901,2
A,C,F,H119376755(-) GAGTAA/GTTTGG 2 -- ut3110Minor allele frequency- N:0.01MN EA NA EU WA 2306
rs120309721,2
C,F,H119375812(+) TTTCAG/AGTGTC 2 -- ut31 ese37Minor allele frequency- A:0.26NA EU EA WA 554
rs37905491,2
C,F,H119377341(+) GCCAGC/GCGGGT 2 A/P mis1 ut31 ese312Minor allele frequency- G:0.17EA NA EU WA 2064
rs37905481,2
C,F,H119376820(+) TCAGAC/GAGCTA 2 -- ut315Minor allele frequency- G:0.17EA EU WA 1906
rs120264091,2
C,F,H119376060(+) AGTTCG/ACATCC 2 -- ut31 ese37Minor allele frequency- A:0.25NA EU EA WA 558
rs170231011,2
C,F,H119377061(+) CCTATA/GGAAAA 2 L/P mis1 ut31 ese37Minor allele frequency- G:0.01NA EU EA WA 546
About this table

HapMap Linkage Disequilibrium images for WARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604733

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/24 PubMed articles for WARS2 gene (see all 24 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 10352 HGNC: 12730 AceView: WARS2 Ensembl:ENSG00000116874 euGenes: HUgn10352
ECgene: WARS2 H-InvDB: WARS2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for WARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for WARS2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for WARS2 Antibodies for WARS2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for WARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Antibodies for WARS2

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