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WAPAL Gene

protein-coding   GIFtS: 54

GC10M088185
wings apart-like homolog (Drosophila)
(Previous name: KIAA0261 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: KIAA0261)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FOE 1, 2, 3, 5
KIAA0261 1, 2, 3, 5
WAPL 1, 2, 3, 5
Descriptions
Friend of EBNA2 protein 3
friend of EBNA2 (Epstein-Barr virus nuclear protein 2) 2
wings apart-like homolog 2
wings apart-like homolog (Drosophila) 2
wings apart-like protein 2
External Ids
HGNC: 232931
Entrez Gene: 230632
UniProtKB: Q7Z5K23
Ensembl: ENSG000000626507
Search outside databases for aliases for WAPAL gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: WAPL_HUMAN, Q7Z5K2
Function: May play a role in cell growth

Gene Wiki entry for WAPAL

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the WAPAL gene  

Entrez Gene cytogenetic band: 10q23.2   Ensembl cytogenetic band:  10q23.2   HGNC cytogenetic band: 10q23.31

WAPAL Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M088185:     (about GC identifiers)

Start:
88,184,993 bp from pter
End:
88,271,552 bp from pter
Size:
86,560 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_030059.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: WAPL_HUMAN, Q7Z5K2 (See protein sequence)
Recommended Name: Wings apart-like protein homolog  
Size: 1190 amino acids; 132946 Da
Subunit: Isoform 2 interacts with Epstein-Barr virus EBNA2
Subcellular location: Isoform 2: Nucleus
Sequence caution: Sequence=CAH73376.1; Type=Erroneous gene model prediction; Sequence=CAI16708.1;
Type=Erroneous gene model prediction;
Secondary accessions: Q5VSK5 Q8IX10 Q92549
Alternative splicing: 3 isoforms:  Q7Z5K2-1   Q7Z5K2-2   Q7Z5K2-3   (Named isoforms)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_055860.1  

    ENSEMBL proteins: 
    ENSP00000345162 ENSP00000361146 ENSP00000361145 ENSP00000298767 ENSP00000263070 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (WAPL)
    Human Recombinant Proteins from Abnova (WAPAL)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for WAPAL: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for WAPAL
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (WAPL), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (WAPAL)
    Novus Biologicals Antibodies for WAPAL

    Assays for WAPAL: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR012502 WAPL


       GeneDecks  WAPAL for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q7Z5K2

    ProtoNet protein and cluster: Q7Z5K2

    1 Blocks protein family: IPB012502 Wings apart-like

    UniProtKB/Swiss-Prot: WAPL_HUMAN, Q7Z5K2
    Similarity: Contains 1 WAPL domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WAPAL)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WAPAL)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_290471

                  Applied Biosystems Silencer® siRNAs for WAPAL

                  Sigma-Aldrich siRNA for WAPAL  
                         Sigma-Aldrich shRNA for WAPAL  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for WAPAL
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015045
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015045
                                     untagged cDNA clone in CMV expression vector: NM_015045 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_015045

    UniProtKB/Swiss-Prot: WAPL_HUMAN, Q7Z5K2
    Function: May play a role in cell growth

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI15231747 17112726 17113138
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for WAPAL (Q7Z5K21 ENSP000003451623) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PDS5BQ9NTI51, ENSP000003138513EBI-1022242, EBI-1175604 STRING (score=.748)
    RAD21O602161EBI-1022242, EBI-80739
    XRN1Q8IZH21EBI-1022242, EBI-372406
    --ENSP000003502263STRING (score=.82)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0044419 interspecies interaction between organisms IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for WAPAL

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (WAPAL)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (WAPAL)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_290471

                  Sigma-Aldrich siRNA for WAPAL  
                         Sigma-Aldrich shRNA for WAPAL  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_015045  

    REFSEQ mRNAs for WAPAL gene: 

    NM_015045.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_015045  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015045
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015045
                                     untagged cDNA clone in CMV expression vector: NM_015045 

    Additional cDNA sequence: AB426836.1 

    12 DOTS entries:

    DT.100804691  DT.95178571  DT.317564  DT.100795230  DT.95069768  DT.100795231  DT.95069767  DT.100795229 
    DT.100795228  DT.92434415  DT.95069764  DT.100795227 

    24/272 AceView cDNA sequences (see all 272 ):

    AW512613 AW237620 BM504085 AI127633 AI469993 AI337178 AF479418 AA630197 
    AA583298 AA703644 AI654429 AI311541 AA325842 AI628928 AI005540 AA748902 
    CB243594 BM478100 AI434763 AW130069 CB218952 BX344489 AI830404 AA682854 

    highest scoring ESTs for WAPAL:

    AA772785 AB065003 AF479418 D87450 AA195661 AA195774 AA233730 AA249352 AA281581 AA281866 

    Unigene Clusters for WAPAL:

    Wings apart-like homolog (Drosophila)
    Hs.203099  [show with all ESTs], Hs.714876
    Unigene Representative Sequences: AB065003, R99673


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for WAPAL

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^ 21a · 21b ·
    SP1:                                      -     -                                                     -                                                         
    SP2:                          -           -     -                                                     -                                                         
    SP3:                                                                                                  -                 -     -                                 
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 21c
    SP1:      
    SP2:      
    SP3:      
    SP4:      
    SP5:      

    About this scheme

    ECgene alternative splicing isoforms for WAPAL

    5 Ensembl transcripts including schematic representations:
    ENST00000342368  ENST00000372076  ENST00000372075  ENST00000298767  ENST00000263070  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    WAPAL expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for WAPAL

    1 / 2 / 3

    6 probe-sets matching WAPAL gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    40086_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D87450 0.80 1.00 0.91 1

    212267_at2, 3 U133-A 1 1.00 1.00 -- -- D87450 0.80 1.00 0.91 1

    212264_s_at2, 3 U133-A 1 1.00 1.00 -- -- BE645850 0.60 1.00 0.82 1

    1554441_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    212267_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    212264_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.203099 Hs.714876

    UniProtKB/Swiss-Prot: WAPL_HUMAN, Q7Z5K2
    Tissue specificity: Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely
    espressed with a high level in skeletal muscle and heart

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for WAPAL gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    WAPAL1   -- wings apart-like homolog (Drosophila) 94.26(n)
    96.03(a)
    612854  XM_859725.1  XP_864818.1 
    mouse
    (Mus musculus)
    Wapal1, 5 145
    wings apart-like homolog (Drosophila)1, 5 91.29(n)1
    93.61(a)1
    2189141  NM_001004436.21  NP_001004436.21 
     AA6750515  AB1673495  (see all 18)
    chicken
    (Gallus gallus)
    WAPAL1   -- wings apart-like homolog (Drosophila) 80.1(n)
    82.29(a)
    423608  XM_421493.2  XP_421493.2 
    zebrafish
    (Danio rerio)
    BM775252.12   --   -- 78.62(n)  BM775252.1 
    worm
    (Caenorhabditis elegans)
    wapl-11   -- WAPL (Drosophila Wings APart-Like cohesin interactor) 42.8(n)
    31.94(a)
    177211  NM_068165.2  NP_500566.1 
    About this table        Species with no ortholog for WAPAL

    ENSEMBL Gene Tree for WAPAL
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/462 NCBI SNPs in WAPAL are shown (see all 462 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 102)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs49342071,2
    A,C,F,H88184509(+) ATATCT/CATAGC 1 -- ng518Minor allele frequency- C:0.37EU EA WA 838
    rs19171601,2
    A,C,F,H88272802(-) CCCCGA/GTTATA 1 -- ng31 tfbs35Minor allele frequency- G:0.07MN EU EA WA 604
    rs108876211,2
    A,C,F,H88267437(+) TTCAAC/TGACCA 1 V/I mis1 ese38Minor allele frequency- T:0.17EU EA WA 836
    rs112020581,2
    C,F,H88272591(+) ACAGCG/ATTCTA 1 -- ng314Minor allele frequency- A:0.21EU EA WA 414
    rs19171611,2
    A,C,F88271759(-) CTAGAG/AGTGGC 1 -- ng312Minor allele frequency- A:0.13NA 182
    rs173310201,2
    F,H88186273(+) GTCTTC/GTCTAA 1 -- ut31 ese37Minor allele frequency- G:0.01NA EU EA WA 558
    --
    rs22897381,2
    C88271324(-) GCCTGC/GGCCCG 1 -- ut51 ese30--------
    --
    rs115940631,2
    H88196006(+) ATCCTC/GCTCCT 1 E/Q mis1 ese3 trp34Minor allele frequency- G:0.00EU EA WA 406
    rs37585191,2
    H88185371(+) GTGCTC/TGACAA 1 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 414
    rs70697991,2
    H88187157(+) AAATCC/TAAACA 1 -- ut314Minor allele frequency- T:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for WAPAL (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610754

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/17 PubMed articles for WAPAL gene (see all 17 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 23063 HGNC: 23293 AceView: KIAA0261 Ensembl:ENSG00000062650 euGenes: HUgn23063
    ECgene: WAPAL H-InvDB: WAPAL
    (According to HUGE)
    About This Section

    HUGE: KIAA0261
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for WAPAL:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for WAPAL-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for WAPAL Antibodies for WAPAL
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for WAPAL Browse small molecules at Sigma-Aldrich
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     Antibodies for WAPAL

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