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Aliases for VRK1 Gene

Aliases for VRK1 Gene

  • Vaccinia Related Kinase 1 2 3
  • Vaccinia-Related Kinase 1 3 4
  • EC 2.7.11.1 4 63
  • PCH1A 3 6
  • Serine/Threonine-Protein Kinase VRK1 3
  • Vaccinia Virus B1R-Related Kinase 1 3
  • PCH1 3

External Ids for VRK1 Gene

Previous GeneCards Identifiers for VRK1 Gene

  • GC14P094774
  • GC14P091080
  • GC14P095253
  • GC14P096333
  • GC14P097263
  • GC14P077447

Summaries for VRK1 Gene

Entrez Gene Summary for VRK1 Gene

  • This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

GeneCards Summary for VRK1 Gene

VRK1 (Vaccinia Related Kinase 1) is a Protein Coding gene. Diseases associated with VRK1 include pontocerebellar hypoplasia type 1a and pontocerebellar hypoplasia type 1. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include identical protein binding and protein serine/threonine kinase activity. An important paralog of this gene is CSNK1G2.

UniProtKB/Swiss-Prot for VRK1 Gene

  • Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

Gene Wiki entry for VRK1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for VRK1 Gene

Genomics for VRK1 Gene

Regulatory Elements for VRK1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for VRK1 Gene

Start:
96,797,304 bp from pter
End:
96,931,722 bp from pter
Size:
134,419 bases
Orientation:
Plus strand

Genomic View for VRK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for VRK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for VRK1 Gene

Proteins for VRK1 Gene

  • Protein details for VRK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99986-VRK1_HUMAN
    Recommended name:
    Serine/threonine-protein kinase VRK1
    Protein Accession:
    Q99986
    Secondary Accessions:
    • Q3SYL2

    Protein attributes for VRK1 Gene

    Size:
    396 amino acids
    Molecular mass:
    45476 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for VRK1 Gene

neXtProt entry for VRK1 Gene

Proteomics data for VRK1 Gene at MOPED

Selected DME Specific Peptides for VRK1 Gene

Post-translational modifications for VRK1 Gene

  • Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis.
  • Ubiquitination at Lys85, Lys112, Lys188, Lys301, Lys307, Lys314, and Lys329
  • Modification sites at PhosphoSitePlus

Other Protein References for VRK1 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for VRK1 (VRK1)

Domains for VRK1 Gene

Gene Families for VRK1 Gene

Protein Domains for VRK1 Gene

Suggested Antigen Peptide Sequences for VRK1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q99986

UniProtKB/Swiss-Prot:

VRK1_HUMAN :
  • Q99986
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
genes like me logo Genes that share domains with VRK1: view

Function for VRK1 Gene

Molecular function for VRK1 Gene

GENATLAS Biochemistry: vaccinia-related kinase 1,widely expressed
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation: Active in presence of Mn(2+), Mg(2+) and Zn(2+), but is not functional with Ca(2+) or Cu(2+). Has a higher affinity for Mn(2+) than for Mg(2+). RAN inhibits its autophosphorylation and its ability to phosphorylate histone H3.
UniProtKB/Swiss-Prot Function: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

Enzyme Numbers (IUBMB) for VRK1 Gene

Gene Ontology (GO) - Molecular Function for VRK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IDA 22194607
GO:0004674 protein serine/threonine kinase activity IDA 21543316
GO:0005515 protein binding IPI 15105425
GO:0005524 ATP binding IEA --
GO:0016772 transferase activity, transferring phosphorus-containing groups --
genes like me logo Genes that share ontologies with VRK1: view
genes like me logo Genes that share phenotypes with VRK1: view

Animal Model Products

CRISPR Products

miRNA for VRK1 Gene

miRTarBase miRNAs that target VRK1

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for VRK1

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for VRK1 Gene

Localization for VRK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for VRK1 Gene

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for VRK1 Gene COMPARTMENTS Subcellular localization image for VRK1 gene
Compartment Confidence
golgi apparatus 5
nucleus 5
cytosol 4
cytoskeleton 2
mitochondrion 2

Gene Ontology (GO) - Cellular Components for VRK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IDA --
GO:0005795 Golgi stack IDA 19103756
genes like me logo Genes that share ontologies with VRK1: view

Pathways for VRK1 Gene

genes like me logo Genes that share pathways with VRK1: view

Pathways by source for VRK1 Gene

1 Cell Signaling Technology pathway for VRK1 Gene

Gene Ontology (GO) - Biological Process for VRK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle TAS --
GO:0006468 protein phosphorylation IDA 22194607
GO:0006897 endocytosis IBA --
GO:0007067 mitotic nuclear division IEA --
GO:0007077 mitotic nuclear envelope disassembly TAS --
genes like me logo Genes that share ontologies with VRK1: view

Compounds for VRK1 Gene

(2) HMDB Compounds for VRK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
genes like me logo Genes that share compounds with VRK1: view

Transcripts for VRK1 Gene

Unigene Clusters for VRK1 Gene

Vaccinia related kinase 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for VRK1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for VRK1 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14
SP1: - -
SP2:
SP3: -
SP4:

Relevant External Links for VRK1 Gene

GeneLoc Exon Structure for
VRK1
ECgene alternative splicing isoforms for
VRK1

Expression for VRK1 Gene

mRNA expression in normal human tissues for VRK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for VRK1 Gene

SOURCE GeneReport for Unigene cluster for VRK1 Gene Hs.422662

mRNA Expression by UniProt/SwissProt for VRK1 Gene

Q99986-VRK1_HUMAN
Tissue specificity: Widely expressed. Highly expressed in fetal liver, testis and thymus.
genes like me logo Genes that share expressions with VRK1: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for VRK1 Gene

Orthologs for VRK1 Gene

This gene was present in the common ancestor of animals.

Orthologs for VRK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia VRK1 35
  • 99.49 (n)
  • 99.75 (a)
VRK1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia VRK1 35
  • 90.4 (n)
  • 90.91 (a)
VRK1 36
  • 86 (a)
OneToOne
dog
(Canis familiaris)
Mammalia VRK1 35
  • 90.07 (n)
  • 90.4 (a)
VRK1 36
  • 90 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Vrk1 35
  • 83.76 (n)
  • 86.29 (a)
Vrk1 16
Vrk1 36
  • 78 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia VRK1 36
  • 75 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia VRK1 36
  • 82 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Vrk1 35
  • 85.44 (n)
  • 87.88 (a)
chicken
(Gallus gallus)
Aves VRK1 35
  • 73.76 (n)
  • 73.16 (a)
VRK1 36
  • 70 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia VRK1 36
  • 64 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia vrk1-prov 35
tropical clawed frog
(Silurana tropicalis)
Amphibia MGC75762 35
vrk1 35
  • 68.37 (n)
  • 69.31 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.4919 35
zebrafish
(Danio rerio)
Actinopterygii vrk1 35
  • 64.33 (n)
  • 63.64 (a)
vrk1 36
  • 58 (a)
OneToOne
wufe16f06 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001933 35
  • 46.81 (n)
  • 39.89 (a)
fruit fly
(Drosophila melanogaster)
Insecta ball 36
  • 25 (a)
ManyToMany
CG8878 36
  • 12 (a)
ManyToMany
BcDNA:LD09009 37
  • 41 (a)
worm
(Caenorhabditis elegans)
Secernentea vrk-1 35
  • 46.02 (n)
  • 35.48 (a)
vrk-1 36
  • 19 (a)
OneToMany
F28B12.3 37
  • 33 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6830 36
  • 47 (a)
OneToMany
Species with no ortholog for VRK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for VRK1 Gene

ENSEMBL:
Gene Tree for VRK1 (if available)
TreeFam:
Gene Tree for VRK1 (if available)

Paralogs for VRK1 Gene

Selected SIMAP similar genes for VRK1 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with VRK1: view

Variants for VRK1 Gene

Sequence variations from dbSNP and Humsavar for VRK1 Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type MAF
rs722869 -- 96,810,668(+) tgact(C/G)agctt intron-variant
rs723810 -- 96,821,633(-) ATAGA(C/T)TGTAA intron-variant
rs736929 -- 96,876,986(+) TGATA(C/T)GATGA intron-variant
rs764300 -- 96,825,905(-) TCATA(A/C)ATTTT intron-variant
rs1885158 -- 96,808,433(-) tgctt(C/G)tgcac intron-variant

Structural Variations from Database of Genomic Variants (DGV) for VRK1 Gene

Variant ID Type Subtype PubMed ID
nsv510650 CNV Loss 20534489
esv260042 OTHER Complex 20981092

Relevant External Links for VRK1 Gene

HapMap Linkage Disequilibrium report
VRK1
Human Gene Mutation Database (HGMD)
VRK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for VRK1 Gene

Disorders for VRK1 Gene

(1) OMIM Diseases for VRK1 Gene (602168)

UniProtKB/Swiss-Prot

VRK1_HUMAN
  • Pontocerebellar hypoplasia 1A (PCH1A) [MIM:607596]: A disorder characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and spinal cord anterior horn cells degeneration resembling infantile spinal muscular atrophy. Additional features include muscle hypotonia, congenital contractures and respiratory insufficiency that is evident at birth. {ECO:0000269 PubMed:19646678}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(4) University of Copenhagen DISEASES for VRK1 Gene

Relevant External Links for VRK1

Genetic Association Database (GAD)
VRK1
Human Genome Epidemiology (HuGE) Navigator
VRK1
genes like me logo Genes that share disorders with VRK1: view

Publications for VRK1 Gene

  1. The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine- 18 within the mdm-2 binding site of the p53 tumour suppressor protein. (PMID: 10951572) Lopez-Borges S. … Lazo P.A. (Oncogene 2000) 3 4 23
  2. Kinetic properties of p53 phosphorylation by the human vaccinia- related kinase 1. (PMID: 11883897) Barcia R. … Lazo P.A. (Arch. Biochem. Biophys. 2002) 3 4 23
  3. Characterization of three paralogous members of the Mammalian vaccinia related kinase family. (PMID: 14645249) Nichols R.J. … Traktman P. (J. Biol. Chem. 2004) 3 4 23
  4. Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK. (PMID: 15105425) Sevilla A. … Lazo P.A. (J. Biol. Chem. 2004) 3 4 23
  5. Spinal muscular atrophy with pontocerebellar hypoplasia is caused by a mutation in the VRK1 gene. (PMID: 19646678) Renbaum P. … Levy-Lahad E. (Am. J. Hum. Genet. 2009) 3 4 23

Products for VRK1 Gene

  • Addgene plasmids for VRK1

Sources for VRK1 Gene

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