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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

VPS35 Gene

protein-coding GIFtS: 56
GCID: GC16M046691

vacuolar protein sorting 35 homolog (S. cerevisiae)

(Previous names: vacuolar protein sorting 35 (yeast homolog), vacuolar protein...)

Explore 9 diseases affiliated with
VPS35 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae)¹ ²		FLJ10752¹
MEM3¹ ² ³ ⁵		Vacuolar Protein Sorting 35 (Yeast Homolog)¹
PARK17¹ ²		Vacuolar Protein Sorting 35 (Yeast)¹
Maternal-Embryonic 3² ³		Vacuolar Protein Sorting-Associated Protein 35²
HVPS35¹		Vesicle Protein Sorting 35³

External Ids:	HGNC: 13487¹	Entrez Gene: 55737²	Ensembl: ENSG00000069329⁷	OMIM: 601501⁵	UniProtKB: Q96QK1³

Export aliases for VPS35 gene to outside databases

Previous GC identifers: GC16M037104 GC16M046634 GC16M046433 GC16M046475 GC16M046474 GC16M045251 GC16M032582

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for VPS35:

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large

multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the

trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex

suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein

interacts directly with VPS35, which serves as the core of the retromer complex. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1

Function: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R

and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric

immunoglobulin receptor (pIgR-pIgA)

Gene Wiki entry for VPS35

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000016.9 NC_018927.1 NT_010498.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the VPS35 gene promoter:
STAT1 STAT4 STAT1beta STAT5A Nkx2-5 Cdc5 STAT1alpha FAC1 STAT2 STAT3
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: VPS35 promoter sequence

Search SABiosciences Chromatin IP Primers for VPS35

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat VPS35

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q12 Ensembl cytogenetic band: 16q11.2 HGNC cytogenetic band: 16q12

VPS35 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
VPS35 gene location

GeneLoc information about chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16M046691: view genomic region (about GC identifiers)

Start:	46,690,054 bp from pter	End:	46,723,430 bp from pter
Size:	33,377 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1 (See protein sequence)

Recommended Name: Vacuolar protein sorting-associated protein 35

Size: 796 amino acids; 91707 Da

Subunit: Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts

directly with VPS26A and VPS26B. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN.

Interacts with GOLPH3

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein

Sequence caution: Sequence=AAG01989.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Sequence=BAA91137.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAB14626.1;

Type=Erroneous initiation; Note=Translation N-terminally shortened;

1 PDB 3D structure from and Proteopedia for VPS35:

2R17 (3D)

Secondary accessions: Q561W2 Q9H016 Q9H096 Q9H4P3 Q9H8J0 Q9NRS7 Q9NVG2 Q9NX80 Q9NZK2

Explore the universe of human proteins at neXtProt for VPS35: NX_Q96QK1

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q96QK1

VPS35 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_060676.2

ENSEMBL proteins:

ENSP00000299138 ENSP00000456274 ENSP00000461469 ENSP00000461423

Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
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	GenScript Custom Purified and Recombinant Proteins Services for VPS35
	Novus Biologicals VPS35 Proteins Novus Biologicals VPS35 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for VPS35

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005768	endosome	IDA	--
GO:0005829	cytosol	IDA	11102511
GO:0016020	membrane	IEA	--
GO:0043231	intracellular membrane-bounded organelle	IDA	--

VPS35 for ontologies About GeneDecksing

VPS35 Antibody Products:

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	Abcam antibodies for VPS35
	Uscn Antibodies for VPS35
	ThermoFisher Antibody for VPS35

Assay Products for VPS35:

	Browse Kits and Assays available from EMD Millipore
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	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for VPS35
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for VPS35

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

VPS35 for domains About GeneDecksing

1 InterPro domain/family:

IPR005378 VPS35

Graphical View of Domain Structure for InterPro Entry Q96QK1

ProtoNet protein and cluster: Q96QK1

1 Blocks protein family: IPB005378 Vacuolar protein sorting-associated protein 35

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1

Similarity: Belongs to the VPS35 family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1

Function: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R

and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric

immunoglobulin receptor (pIgR-pIgA)

miRNA Products:		OriGene 3'-UTR Clone: VPS35 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat VPS35
		8/12 QIAGEN miScript miRNA Assays for microRNAs that regulate VPS35 (see all 12):
		hsa-miR-569 hsa-miR-3688-3p hsa-miR-513a-3p hsa-miR-586 hsa-miR-488 hsa-miR-371-5p hsa-miR-3161 hsa-miR-633

		SwitchGear 3'UTR luciferase reporter plasmid: VPS35 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for VPS35 (see all 6) OriGene siRNA: VPS35
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat VPS35

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for VPS35

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for VPS35 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for VPS35 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: VPS35 (NM_018206)
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		DNA2.0 Custom Codon Optimized Gene Synthesis Service for VPS35
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Cell Line Products:		GenScript Custom overexpressing Cell Line Services for VPS35
		Search LifeMap BioReagents cell lines for VPS35

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for VPS35

Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IPI	19553991

VPS35 for ontologies About GeneDecksing

1 GenomeRNAi human phenotype for VPS35:

Cell cycle / mitosis defect

Animal Models:
4 MGI mutant phenotypes (inferred from 1 allele) (MGI details for Vps35):

behavior/neurological

mortality/aging

nervous system

other

VPS35 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for VPS35

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/107 Interacting proteins for VPS35 (Q96QK1^{2, 3} ENSP00000299138⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 107)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ZNF512B	Q96KM6²^,³	MINT-60836 I2D: score=2
HIST1H2BC	P62807³	I2D: score=1
HIST1H2BE	P62807³	I2D: score=1
HIST1H2BF	P62807³	I2D: score=1
HIST1H2BG	P62807³	I2D: score=1

About this table

Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008219	cell death	IEA	--
GO:0015031	protein transport	IEA	--
GO:0042147	retrograde transport, endosome to Golgi	NAS	11102511

VPS35 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for VPS35
Search CenterWatch for drugs/clinical trials and news about VPS35

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for VPS35 gene:

NM_018206.4

Unigene Cluster for VPS35:

Vacuolar protein sorting 35 homolog (S. cerevisiae)

Hs.454528 [show with all ESTs]

Unigene Representative Sequence: AK025774

11 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000299138(uc002eef.4) ENST00000568784(uc002eed.3 uc002eee.3)

ENST00000562420 ENST00000568642 ENST00000569950 ENST00000565228 ENST00000563884

ENST00000568612 ENST00000561713 ENST00000568191 ENST00000563984

miRNA Products:		OriGene 3'-UTR Clone: VPS35 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat VPS35
		8/12 QIAGEN miScript miRNA Assays for microRNAs that regulate VPS35 (see all 12):
		hsa-miR-569 hsa-miR-3688-3p hsa-miR-513a-3p hsa-miR-586 hsa-miR-488 hsa-miR-371-5p hsa-miR-3161 hsa-miR-633

		SwitchGear 3'UTR luciferase reporter plasmid: VPS35 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for VPS35 (see all 6) OriGene siRNA: VPS35
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat VPS35

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for VPS35 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for VPS35 OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: VPS35 (NM_018206)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for VPS35
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat VPS35

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for VPS35 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat VPS35
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat VPS35
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat VPS35

Additional cDNA sequence:

AF175265.1 AF183418.1 AF186382.1 AF191298.2 AK000395.1 AK001614.1 AK023650.1 AK025774.1

AK223218.1 AL136888.1 AL512769.1 AY007112.1 BC002414.2 BC010362.1 BC041367.2 BC093036.1

24/34 DOTS entries (see all 34):

DT.454462 DT.100881305 DT.95369874 DT.100881306 DT.100725496 DT.100881303 DT.100881298 DT.100881299

DT.211205 DT.95369860 DT.120665384 DT.120665392 DT.120665380 DT.120665354 DT.120665387 DT.95369869

DT.97860347 DT.101959592 DT.120665357 DT.120665389 DT.120665427 DT.92453902 DT.100881297 DT.100881300

621 AceView cDNA sequences (see top 24):

CD558865 AI989821 CA444729 AI280716 CB112878 AI866449 BX379816 AL041076

AF183418 BE909765 AW672736 BQ433648 CB137428 CB988432 AI302924 BX422707

AW511973 AL043647 AW518217 BC002414 AL512769 T33241 F10817 AI524301

BG255771 AA149212 BM833729 CR618276 BQ435440 BQ437747 AW516821 AI654848

BI962577 BU621624 BM806626 BF795110 BQ929887 BM770213 AU132465 BM991546

AF175265 CA420509 BP341904 AK023650 BM468336 CB321761 AL136888 AA296476

AA181323 BG319524 BQ431694 BM977321 AA251150 BE466772 BM928088 CA406886

T16413 BP375035 AU120957 BU733209 AI620975 BI335315 AA541333 BQ921207

BM541308 BU686147 CD366481 CD244174 CB305481 AI961227 AW160786 AI523470

CN480613 CB216653 CD676147 CB130812 CA415579 CB134777 CB137461 BQ893715

BQ645934 CB131486 BE326700 BE093063 BM820386 AI206212 AI692468 BM127523

CN484592 BM450432 AI245882 BI324933 BM991555 Z40752 AI719670 AW052052

N36476 AI127129 BM996006 BP375284 N51792 BP378317 BU623352 BP376844

BQ962909 NM_018206 BM467705 BM707671 BM542572 BE646250 CR626386 CD723193

BE093066 AU121505 CB152433 BG261298 AL041719 AU139834 CR602924 AW161618

BF979531 BG502016 AW901917 BP352500 AI289422 AK000395 BI090686 BX330153

BF677643 BU145541 AA864578 CD686499 BQ574179 CD643652 BG178763 BG114316

BF701143 AI673351 BX646182 BM709725 BG171495 AW630317 CD300333 BM194470

BI561291 AA360793 BQ225946 BM750925 AW630928 BM541872 AA403194 AW628269

BG714776 CA394572 BM804442 BU933025 AA251519 AL041718 BP342601 N40972

CB984865 BU150899 W31591 BM986986 BM806562 BQ220305 AI807207 CB157030

BU630665 BF026058 BG703974 AW189264 BE892474 AL577565 BU533821 AA620397

BP343648 BI457749 BG715723 BE538626 AF191298 N30125 AI159966 BM834102

BM972389 BC010362 AI185855 BG723314 AL048887 BG024739 AI050899 AV723740

BP355224 BM476716 AI636656 CD512066 BE897913 CB132547 BG953381 BM998239

AW408591 BG541981 AA307735 N93658 BE073476 AA055303 AV725193 AI889248

BX336338 BU508813 AL554834 AL520061 BM905260 BM835759 BM543100 BM854718

BE395179 BQ645978 BM789533 CA435922 AA890482 W32329 AI749275 W56368

T61967 BM147919 AI686865 AF186382 BU182973 BM827983 BM477139 BU190343

AA831120 AI708274 AI440100 AW238853 AI917466 AW274921 AW512970 BI093817

BG613850 AW007106 BC041367 BM921520 CB987974 BM802770 BX647213 BI860869

CB987300 BM827349 N49248 BM818063 AI288516 BP366434 BQ187885 AI796852

BX376177 AL549426 AI272822 BE875987 BG402951 BG178442 T10104 BU569817

BG492877 N70561 BG828838 BI771669 CA392468 AI637813 CB147822 BG260872

N30446 AA406165 BE702110 AU124131 AW901909 T08054 BI460866 W44915

BI253392 BG430749 N26528 CD102277 BM801674 BP345764 BM834551 BG770288

AW954231 CD653985 BQ277468 CD103561 BG575031 AY007112 AA862892 AI830546

BP340697 BE702141 CB988002 AL043648 AW440447 CB162604 AI288507 AU150040

BG392451 BX405674 H60068 BE886050 BX377177 AI168077 CB131466 W15519

BM047370 BG024159 BP376139 N42359 N47691 AI131444 BP383260 AI571463

BG035942 AA082994 BG503515 BM542721 BG826745 AU135687 BF668100 BX956853

T62705 AU280518 AA031795 AI289860 BG772101 BM830177 AL524539 AA031817

BX437996 BP379448 AA033599 AK001614 BQ217411 BI493456 CD709195 AF086051

AV733084 BI544999 N56051 AI459439 BI089135 R39843 BM827042 AU156223

BF669198 BQ217595 BF364128 BG476669 AI131043 AI493560 BE702249 CB984989

BE242252 AA329330 BU564477 AA620415 BM152761 N29982 AA039348 AW081564

AA299018 CD704874 AA600055 AW615058 AA634345 AI474574 AA633723 BG336654

H97568 W24380 CF454899 BQ898114 AW003494 AI032243 CD695423 H39930

BG287432 AA744797 BM473103 BQ216974 AI245315 BM821895 BP364153 AU156622

AU151924 BG254048 BG620042 BP378738 BI460955 BI600044 AU252437 BQ028391

N51700 AA916752 BE613721 BM971213 BG027938 AL565605 AW023587 BU941579

R36929 BU928132 BE616514 AA031816 N40965 BG769280 T36199 BG927480

BG699257 N35639 AA401204 BP342313 BU678580 AW073425 W19227 AU252436

BI092226 CD629052 AW581721 AA121016 AL580792 CA444785 BE257385 BU678673

BM994100 Z45021 T61905 AI590372 BF105264 BF380403 W05431 AU147304

BU430146 BQ939792 W56579 BF665284 AU146882 H28444 BG423435 AW135147

AU153985 W02745 BE166329 BE787649 BG676344 AA126464 BE877830 AI263591

BG719009 BI458947 AA568447 AA149211 CD690734 BP383377 BE855712 BF672137

BG716326 AA350109 H13224 BE262380 W93852 BI562014 BE702018 BE546943

T57671 H13593 CA311543 R14188 R37791 F13217 BF572420 CD688624

AU155252 BM459629 AA856703 AU138790 T62855 BF311564 AA398872 BI460799

BE543326 AI692469 H50806 AI866263 AA055212 N86136 BE466076 BQ356290

AW938384 AA435640 H00125 AA083151 CD690103 AL039920 BE615639 BI087581

BG925380 R15057 AW966869 BF993715 AU152555 AL534646 CD688376 BI004038

BF966258 BM749074 BE564637 AA976285 D31298 AU151321 AA852909 BE769316

R86310 T91235 AI869799 BF359812 BF896222 BF355660 CB049307 BE870816

T57711 AA574268 BF325574 AA714535 BQ067903 CK002409 N83144 AA179982

AA526893 AU153342 BF819158 BE702244 BP432755 W25420 BG705923 BE616327

BG250413 T10105 BE958191 BM475104 BQ359846 BF680039 BE702185 CD707351

BE789874 CD698293 AI867852 BF325563 F13798 BE702103 N36319 AI962017

BF359828 H50704 BX400023 N47690 AU076848 R37470 AI351127 BQ051084

BU536036 BF325560 BX374816 BF745498 AW004674 BF377706 AA354850 BF987933

BU173421 BF325573 AI240253 BE084609 BF361741 N35736 BG749678 AA033600

AA888823 CD676752 R86309 AA702301 BE789081 BG913196 AL559100 BF757803

W24756 BI094051 AL573730 BG003662 BI463143 BF745421 BU854597 AW088454

BQ312357 R41491 BI257053 H59412 BF247277 BF029263 BE702143 BE810778

BF325561 BE810775 BE810783 BE810784 BF743952 T75430 BQ318347 BF677134

AW151225 BG435890 AA083694 BE077363 R13695

GeneLoc Exon Structure

5/14 Alternative Splicing Database (ASD) splice patterns (SP) for VPS35 (see all 14) About this scheme

ExUns:	1a	·	1b	^	2a	·	2b	^	3	^	4a	·	4b	^	5a	·	5b	^	6a	·	6b	^	7	^	8a	·	8b	^	9a	·	9b	·	9c	·	9d	^	10a	·	10b	·	10c	·	10d	^	11	^	12	^	13	^	14	^
SP1:									-						-		-						-		-
SP2:															-		-						-		-
SP3:															-		-		-		-		-
SP4:									-				-		-		-		-				-		-
SP5:					-		-		-						-		-						-		-

ExUns:	15	^	16	^	17	^	18	^	19a	·	19b	^	20a	·	20b	^	21
SP1:
SP2:
SP3:
SP4:
SP5:

ECgene alternative splicing isoforms for VPS35

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

VPS35 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TTGTCCTCTG

About this image
See VPS35 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for VPS35

SOURCE GeneReport for Unigene cluster: Hs.454528

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1

Tissue specificity: Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis,

ovary, small intestine, kidney and colon

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In Situ
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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for VPS35 gene from 10/37 species (see all 37) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Vps35^{1 , 5}	vacuolar protein sorting 35^{1, 5}	91.46(n)¹ 99.37(a)¹		8 (41.61 cM)⁵ 65114¹ NM_022997.4¹ NP_075373.1¹ 85260392⁵
chicken (Gallus gallus)	Aves	VPS35¹	vacuolar protein sorting 35 homolog (S. cerevisiae)	84.21(n) 97.99(a)		415750 NM_001005842.1 NP_001005842.1
lizard (Anolis carolinensis)	Reptilia	VPS35⁶	--	97(a)	1 ↔ 1	LGc(3607708-3631393)
African clawed frog (Xenopus laevis)	Amphibia	Xl.8144²	Xenopus laevis transcribed sequence with strong similarity more	80.55(n)		CA988274.1
zebrafish (Danio rerio)	Actinopterygii	vps35¹	vacuolar protein sorting 35 (yeast)	76.76(n) 93.97(a)		561697 NM_001025517.2 NP_001020688.2
fruit fly (Drosophila melanogaster)	Insecta	CG5625³ Vps35¹	vesicle-mediated transport³ Vacuolar protein sorting 35¹	58(a)³ 59.71(n)¹ 62.63(a)¹		58C7³ 37536¹ NM_137807.3¹ NP_611651.4¹
worm (Caenorhabditis elegans)	Secernentea	vps-35¹	Protein VPS-35	55.12(n) 49.94(a)		174000 NM_062779.3 NP_495180.2
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	VPS35(YJL154C)⁴ VPS35¹	Endosomal subunit of membrane-associated retromer complex more⁴ Vps35p¹	49.82(n)¹ 37.57(a)¹		10(133935-131101)⁴ 853287^{1, 4} NP_012381.1^{1, 4}
thale cress (Arabidopsis thaliana)	eudicotyledons	VPS35B¹	vacuolar sorting protein 35	52.57(n) 44.88(a)		843918 NM_106235.4 NP_177713.3
rice (Oryza sativa)	Liliopsida	AK069591.1²	--	72.38(n)		AK069591.1

ENSEMBL Gene Tree for VPS35 (if available)
TreeFam Gene Tree for VPS35 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for VPS35 gene

1 SIMAP similar gene for VPS35 using alignment to 4 protein entries: VPS35_HUMAN (see all proteins):

DKFZp686O2462

VPS35 for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for VPS35
PGOHUM00000249099

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 16 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs188286943^1,2	C,F	pathogenic	34973276(+)	`GGAATC/TGCTGA`	2	N D	mis¹		3		EU	5092
rs184277092^1,2	C	unknown	34966712(+)	`AATCCG/ACTGAT`	2	/R /W	mis¹		3		EU	5096
rs11550461^1,2	C	--	32582925(+)	`GTGAAT/CAATTT`	1	--	ut3¹		1		NA	2
rs192419029^1,2	F	--	32583175(+)	`CTCCAG/TTGTGT`	2	M L	mis¹		2		EU	3754
rs1645943^1,2	C,H	--	32583369(-)	`TAACAG/AAAGCT`	1	--	int¹		7		MN NS EA NA	606
rs8045759^1,2	C,	--	32583966(+)	`tcaagC/G/Tgattc`	1	--	int¹		0	--	--	--	--
rs111681844^1,2	C,	--	32584022(+)	`CCACGA/CCTAGC`	1	--	int¹		1		CSA	1
rs111445265^1,2	C,	--	32584768(+)	`TTGTTA/CATCAG`	1	--	int¹		0	--	--	--	--
rs77476733^1,2	C,F,	--	32585512(+)	`ACACAT/CTCAGT`	1	--	int¹		1		EA	120
rs34687100^1,2	C,F	--	32585639(+)	`ATGCGA/TCTGTC`	2	D V	mis¹		3		NA	72

HapMap Linkage Disequilibrium report for VPS35 (46690054 - 46723430 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for VPS35: --
Human Gene Mutation Database (HGMD): VPS35

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing VPS35

DNA2.0 Custom Variant and Variant Library Synthesis for VPS35

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

VPS35 for disorders About GeneDecksing

OMIM gene information: 601501 OMIM disorders: --

UniProtKB/Swiss-Prot: VPS35_HUMAN, Q96QK1

Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17) [MIM:614203]. PARK17 is an autosomal

dominant, adult-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder

characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically

significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the

substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving

neurons in various areas of the brain

9 diseases for VPS35: About MalaCards

tremor parkinson's disease hepatocellular carcinoma alzheimer's disease

colorectal cancer breast cancer schizophrenia carcinoma

malaria

1 disease from the University of Copenhagen DISEASES database for VPS35:

Parkinson's disease

Export disorders for VPS35 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for VPS35 gene, integrated from 9 sources (see all 75):
(articles sorted by number of sources associating them with VPS35)

Utopia: connect your pdf to the dynamic
world of online information

VPS35 mutations in Parkinson disease. (PubMed id 21763482)^{1, 2, 3} Vilarino-Guell C.... Farrer M.J. (2011)
Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. (PubMed id 11112353)^{1, 2, 3} Zhang P.... Zhao S. (2000)
Human homologues of yeast vacuolar protein sorting 29 and 35. (PubMed id 11062004)^{1, 2, 9} Edgar A.J. and Polak J.M. (2000)
Identification of VPS35 mutations replicated in French families with Parkinson disease. (PubMed id 22517097)^{1, 2} Lesage S.... Brice A. (2012)
A mutation in VPS35, encoding a subunit of the retromer complex, causes late-onset Parkinson disease. (PubMed id 21763483)^{1, 2} Zimprich A.... Strom T.M. (2011)
GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. (PubMed id 19553991)^{1, 2} Scott K.L.... Chin L. (2009)
Functional architecture of the retromer cargo-recognition complex. (PubMed id 17891154)^{1, 2} Hierro A.... Hurley J.H. (2007)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
Exportin 7 defines a novel general nuclear export pathway. (PubMed id 15282546)^{1, 2} Mingot J.-M.... Goerlich D. (2004)
The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor. (PubMed id 15247922)^{1, 2} Verges M.... Mostov K.E. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 55737	HGNC: 13487	AceView: VPS35	Ensembl:ENSG00000069329	euGenes: HUgn55737
ECgene: VPS35	H-InvDB: VPS35

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for VPS35	Pharmacogenomics, SNPs, Pathways

Intellectual Property for VPS35 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for VPS35 gene:
Search GeneIP for patents involving VPS35

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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VPS35 Gene

vacuolar protein sorting 35 homolog (S. cerevisiae)

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VPS35 Gene

vacuolar protein sorting 35 homolog (S. cerevisiae)

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