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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

VPS33A Gene

protein-coding   GIFtS: 52
GCID: GC12M122716

vacuolar protein sorting 33 homolog A (S. cerevisiae)

(Previous name: vacuolar protein sorting 33A (yeast) )
 Explore 2 diseases affiliated with
VPS33A via our new
 Human Malady Compendium 
Biological research products
for VPS33A
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Vacuolar Protein Sorting 33 Homolog A (S. Cerevisiae)1 2
HVPS33A1
Vacuolar Protein Sorting 33A (Yeast)1
Vacuolar Protein Sorting 33A2
Vacuolar Protein Sorting-Associated Protein 33A2

External Ids:    HGNC: 181791   Entrez Gene: 650822   Ensembl: ENSG000001397197   OMIM: 6100345   UniProtKB: Q96AX13   

Export aliases for VPS33A gene to outside databases

Previous GC identifers: GC12M121416 GC12P122609 GC12M122524 GC12M121153 GC12M121241 GC12M121282 GC12M119676


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for VPS33A:
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct
organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in
vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to
the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late
endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome
pathway. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: VP33A_HUMAN, Q96AX1
Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane
docking/fusion reactions of late endosomes/lysosomes (By similarity)

Gene Wiki entry for VPS33A


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009755.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the VPS33A gene promoter:
         AhR   USF1   Pbx1a   CUTL1   MIF-1   Tal-1beta   E47   USF-1   FOXO1a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidVPS33A promoter sequence
   Search SABiosciences Chromatin IP Primers for VPS33A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat VPS33A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24.31   Ensembl cytogenetic band:  12q24.31   HGNC cytogenetic band: 12q24.31

VPS33A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
VPS33A gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M122716:  view genomic region     (about GC identifiers)

Start:
122,715,292 bp from pter      End:
122,751,068 bp from pter
Size:
35,777 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: VP33A_HUMAN, Q96AX1 (See protein sequence)
Recommended Name: Vacuolar protein sorting-associated protein 33A  
Size: 596 amino acids; 67611 Da
Subunit: Large heterooligomeric complex together with VPS11, VPS16 and VPS18. Interacts with STX7 (By similarity).
Interacts with VIPAS39
Subcellular location: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Lysosome
membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Note=Cytoplasmic, peripheral membrane protein
associated with late endosomes/lysosomes (By similarity)
Sequence caution: Sequence=BAB15570.1; Type=Frameshift; Positions=140;
Secondary accessions: Q547V4 Q9H5Q0

Explore the universe of human proteins at neXtProt for VPS33A: NX_Q96AX1

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96AX1

  • VPS33A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_075067.2  
    ENSEMBL proteins: 
     ENSP00000267199   ENSP00000442392   ENSP00000439255   ENSP00000442951  

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    Uscn Proteins for VPS33A

    Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005765lysosomal membrane IEA--
    GO:0005769early endosome IDA19109425
    GO:0005770late endosome IDA19109425
    GO:0030897colocalizes with HOPS complex IDA19109425
    GO:0031902late endosome membrane IEA--


    VPS33A for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    VPS33A for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR027121 VPS33
     IPR001619 Sec1-like

    Graphical View of Domain Structure for InterPro Entry Q96AX1

    ProtoNet protein and cluster: Q96AX1

    1 Blocks protein family: IPB001619 Sec1-like protein

    UniProtKB/Swiss-Prot: VP33A_HUMAN, Q96AX1
    Similarity: Belongs to the STXBP/unc-18/SEC1 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: VP33A_HUMAN, Q96AX1
    Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane
    docking/fusion reactions of late endosomes/lysosomes (By similarity)

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmidVPS33A 3' UTR sequence
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI19109425


    VPS33A for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for VPS33A:
     Synthetic lethal with Ras 

    Animal Models:
         6 MGI mutant phenotypes (inferred from 1 allele(MGI details for Vps33a):
     hematopoietic system  homeostasis/metabolism  integument  pigmentation  renal/urinary system 
     vision/eye 

    VPS33A for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for VPS33A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 11)

    5/563 Interacting proteins for VPS33A (Q96AX12, 3 ENSP000002671994) via UniProtKB, MINT, STRING, and/or I2D (see all 563)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EEF1A1P681042, 3MINT-7947479 I2D: score=1 
    FEN1P397482, 3MINT-7947479 I2D: score=1 
    HIP1RO751462, 3MINT-7947479 I2D: score=1 
    HSP90AA1P079002, 3MINT-7947479 I2D: score=1 
    MON1BQ7L1V23, ENSP000002482484I2D: score=1 STRING: ENSP00000248248
    About this table

    Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006904vesicle docking involved in exocytosis IEA--
    GO:0015031protein transport IEA--
    GO:0016192vesicle-mediated transport IDA15790593
    GO:0030220platelet formation ISS--
    GO:0032400melanosome localization IDA15790593


    VPS33A for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for VPS33A
    Search CenterWatch for drugs/clinical trials and news about VPS33A / VP33A 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for VPS33A gene: 
    NM_022916.4  

    Unigene Cluster for VPS33A:

    Vacuolar protein sorting 33 homolog A (S. cerevisiae)
    Hs.592009  [show with all ESTs]
    Unigene Representative Sequence: NM_022916
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000267199(uc001ucd.3) ENST00000543633 ENST00000541169 ENST00000536212
    ENST00000540669 ENST00000544349 ENST00000542790 ENST00000542310 ENST00000451053


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    hsa-miR-4311 hsa-miR-3653 hsa-miR-520a-5p hsa-miR-1205 hsa-miR-27b* hsa-miR-1184 hsa-miR-544 hsa-miR-3658
    SwitchGear 3'UTR luciferase reporter plasmidVPS33A 3' UTR sequence
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    Additional cDNA sequence: 

    AF439857.1 AK026048.1 AK026840.1 AK315545.1 BC016617.1 

    12 DOTS entries:

    DT.308181  DT.92430696  DT.40190593  DT.100749478  DT.213186  DT.91686113  DT.100728586  DT.92430706 
    DT.95267531  DT.95267532  DT.95267535  DT.95345135 

    24/436 AceView cDNA sequences (see all 436):

    AW296829 CR621745 CB113242 BF848063 BC011909 BU620809 BU620721 CB118849 
    AI268019 BU678922 AI885240 BX370573 BI259661 BQ021141 AA326319 CB528370 
    AA024961 BU170405 CB159850 BC004417 CR596276 CR598154 BC016617 AA744760 

    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for VPS33A (see all 9)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15
    SP1:              -     -                             -                       -     -     -                                                               
    SP2:                                                                          -     -     -                                                               
    SP3:                                                                                                                                                      
    SP4:                                                                                                                                                      
    SP5:              -     -                                                                                                                                 


    ECgene alternative splicing isoforms for VPS33A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    VPS33A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGAATACAGA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See VPS33A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for VPS33A

    SOURCE GeneReport for Unigene cluster: Hs.592009
        SABiosciences Custom PCR Arrays for VPS33A
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for VPS33A gene from 6/30 species (see all 30)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves VPS33A1 vacuolar protein sorting 33 homolog A (S. cerevisiae) 78.79(n)
    91.75(a)
      416861  XM_415153.2  XP_415153.1 
    lizard
    (Anolis carolinensis)
    Reptilia VPS33A6
    --
    91(a)
    1 → many
    GL343423.1(35702-73204)
    zebrafish
    (Danio rerio)
    Actinopterygii vps33a1 vacuolar protein sorting 33A 73.29(n)
    84.68(a)
      448942  NM_001099973.1  NP_001093443.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta car1 , 3 ommochrome biosynthesis SNARE binding3
    carnation1
    43(a)3
    49.31(n)1
    45.53(a)1
      1 18D13
    329471  NM_167664.21  NP_728266.11 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons ATVPS331 vacuolar protein sorting-associated protein 33-like more 48.14(n)
    39.34(a)
      824651  NM_001161207.1  NP_001154679.1 
    rice
    (Oryza sativa)
    Liliopsida Os04g02230001 hypothetical protein 49.03(n)
    39.89(a)
      4335201  NM_001058805.1  NP_001052270.1 


    ENSEMBL Gene Tree for VPS33A (if available)
    TreeFam Gene Tree for VPS33A (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for VPS33A gene
    VPS33B2  ENSG000002568612  
    8 SIMAP similar genes for VPS33A using alignment to 5 protein entries:     VP33A_HUMAN (see all proteins):
    TCF4    DNAPTP3    ZNF212    GLT8D2    NF2    C4orf21
    PLEKHH2    VPS33B

    VPS33A for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/707 NCBI SNPs in VPS33A are shown (see all 707    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs734217191,2
    C,--122715620(+) GTTGCC/TCCTGC 1 -- int12Minor allele frequency- T:0.07WA 120
    rs1863219411,2
    --122715634(+) CTGTGA/GGACCA 1 -- int10--------
    rs1153799501,2
    --122715753(+) ATTACT/CTCAAA 1 -- int11Minor allele frequency- C:0.01WA 118
    rs1495779471,2
    --122715850(+) GCTGAA/TCACAT 1 -- int10--------
    rs1442879321,2
    --122715861(+) AGAGCA/GTAAAC 1 -- int10--------
    rs1487555711,2
    --122715929(+) GCAAAC/TTTGCC 1 -- int10--------
    rs1916032591,2
    --122715950(+) GTTGCA/GACAGT 1 -- int10--------
    rs20682231,2
    C,F,A,H,--122716068(+) GATGCG/ACCTTG 1 -- int121Minor allele frequency- A:0.30NS EA NA WA 2528
    rs284397491,2
    C,F,H,--122716189(+) AATGGA/GTTATC 1 -- ut3112Minor allele frequency- G:0.05NS NA WA 1588
    rs1395786461,2
    C,--122716290(+) TGATT-/TTC   
       TTCTA
    TTCTT
    1 -- ut310--------

    HapMap Linkage Disequilibrium report for VPS33A (122715292 - 122751068 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for VPS33A: --

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    VPS33A for disorders           About GeneDecksing

    OMIM gene information: 610034    OMIM disorders: --

    2 diseases for VPS33A:    About MalaCards
    chediak-higashi syndrome    hermansky-pudlak syndrome

    2 diseases from the University of Copenhagen DISEASES database for VPS33A:
    Hermansky-Pudlak syndrome     ARC syndrome

    Export disorders for VPS33A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for VPS33A gene, integrated from 9 sources (see all 22):
    (articles sorted by number of sources associating them with VPS33A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery. (PubMed id 19109425)1, 2 Zhu G.D....L'hernault S.W. (2009)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. The mouse organellar biogenesis mutant buff results from a mutation in Vps33a, a homologue of yeast vps33 and Drosophila carnation. (PubMed id 12538872)1, 2 Suzuki T.... Spritz R.A. (2003)
    5. Molecular cloning and characterization of human VPS18, VPS11, VPS16, and VPS33. (PubMed id 11250079)1, 3 Huizing M.... Kraemer H. (2001)
    6. Comparative evolutionary analysis of VPS33 homologues: genetic and functional insights. (PubMed id 15790593)1, 9 Gissen P....Maher E.R. (2005)
    7. Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
    8. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    9. Targeting protein-trafficking pathways alters melanoma treatment sensitivity. (PubMed id 22203954)1 Huang Z.M....Wei M.L. (2012)
    10. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
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      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 65082 HGNC: 18179 AceView: DIABLOandVPS33A Ensembl:ENSG00000139719 euGenes: HUgn65082
    ECgene: VPS33A H-InvDB: VPS33A

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for VPS33A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for VPS33A gene:
    Search GeneIP for patents involving VPS33A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

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