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Aliases & Descriptions for VPS18
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| KIAA1475 1, 2, 3 | | PEP3 1, 2 | | hVPS18 3 |
| | | Descriptions |
|---|
| vacuolar protein sorting 18 2 | | vacuolar protein sorting 18 homolog (S. cerevisiae) 2 | | vacuolar protein sorting protein 18 1, 2 |
|
| | Search outside databases for aliases for VPS18 genePrevious GC identifers: GC15P036554 GC15P034082 GC15P038765 GC15P038902 |
Summaries for VPS18(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for VPS18: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq] UniProtKB/Swiss-Prot: VPS18_HUMAN, Q9P253Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomesGene Wiki entry for VPS18 |
Genomic Location for VPS18
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the VPS18 gene 
Entrez Gene cytogenetic band: 15q14-q15 Ensembl cytogenetic band: 15q15.1 HGNC cytogenetic band: 15q14-q15VPS18 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 15 GeneLoc Exon Structure GeneLoc location for GC15P038974:
(about GC identifiers)
Start:
|
38,973,920 bp from pter |
End:
|
38,983,465 bp from pter |
Size:
|
9,546 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000015.8 NT_010194.16
| Proteins for VPS18
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: VPS18_HUMAN, Q9P253 (See
protein sequence)Recommended Name: Vacuolar protein sorting-associated protein 18 homolog Size: 973 amino acids; 110186 Da
Subunit: Interacts with HOOK1 (By similarity). Large hetero-oligomeric complex together with VPS11, VPS16 and VPS33A. Interacts with STX7
Subcellular location: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes
Secondary accessions: Q8TCG0 Q96DI3 Q9H268Alternative splicing: 2 isoforms: Q9P253-1 Q9P253-2 Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_065908.1
ENSEMBL proteins: ENSP00000220509
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
5 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for VPS18: Assays for VPS18: | Protein
Domains/ Families for VPS18(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q9P253
ProtoNet protein and cluster: Q9P253 2 Blocks protein families: IPB001841 Zn-finger IPB007810 Pep3/Vps18/deep orange
UniProtKB/Swiss-Prot: VPS18_HUMAN, Q9P253Similarity: Belongs to the VPS18 familySimilarity: Contains 1 clathrin repeatSimilarity: Contains 1 RING-type zinc finger | Gene Function for VPS18
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020857
Applied Biosystems Silencer® siRNAs for VPS18
Sigma-Aldrich siRNA for VPS18  Sigma-Aldrich shRNA for VPS18  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020857                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_020857                                  untagged cDNA clone in CMV expression vector: NM_020857 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_020857
UniProtKB/Swiss-Prot: VPS18_HUMAN, Q9P253Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes4 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for VPS18
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
5/6 Interacting proteins for VPS18 (ENSP000002205093 Q9P2531) via UniProtKB, MINT, and/or STRING (see all 6
)About this table
2 Gene Ontology (GO) biological process terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007032 | endosome organization |
IEA | -- | | GO:0015031 | protein transport |
IEA | -- | About this table
|
Drugs & Compounds for VPS18(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for VPS18
|
Transcripts for VPS18(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020857
Sigma-Aldrich siRNA for VPS18  Sigma-Aldrich shRNA for VPS18  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_020857 REFSEQ mRNAs for VPS18 gene: NM_020857.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_020857               OriGene GFP tagged cDNA clone in CMV expression vector: NM_020857                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_020857                                  untagged cDNA clone in CMV expression vector: NM_020857  Additional cDNA sequence: AB040908.1 AF308802.1 AL713725.1 BC001513.2 5 DOTS entries: DT.412982 DT.97845994 DT.70104280 DT.75103176 DT.95082977 24/164 AceView cDNA sequences (see all 164
):AW188900 BQ018419 CA413853 AL524858 BM314059 BU159902 CK905184 AF308802 BM987207 NM_020857 AI560899 CD635831 BQ058903 BQ058274 BM972971 AW511689 CB853629 BC001513 BX493534 AB040908 AI587540 BM669495 BQ057408 AI928969
highest scoring ESTs for VPS18:AA082935 AA037759 AA039249 AA420503 AA420504 AA476967 AA707703 AA779135 AA879408 AB040908 Unigene Cluster for VPS18: Vacuolar protein sorting 18 homolog (S. cerevisiae) Hs.23876 [show with all ESTs]Unigene Representative Sequence: AL713725
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for VPS18
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | |
| SP1: | |   | |   | |   | - |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | - |   | |   | - |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for VPS18
1 Ensembl transcript including schematic representation: ENST00000220509
|
Expression for VPS18
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| VPS18 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for VPS18
1 / 2 / 3 4 probe-sets matching VPS18 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: CTTTTGTGCA
SOURCE GeneReport for Unigene cluster: Hs.23876 UniProtKB/Swiss-Prot: VPS18_HUMAN, Q9P253Tissue specificity: Ubiquitous. Expression was highest in heart and low in lung |
Orthologs for VPS18
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for VPS18 gene from 5/15 species (see all 15
)
About this table Species with no ortholog for VPS18
ENSEMBL Gene Tree for VPS18 | Paralogs for VPS18(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| -- |
SNPs/Variants for VPS18(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for VPS18 (up to first 250kb)
|
Disorders & Mutations for VPS18
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 608551
|
Medical News for VPS18(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for VPS18 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/15 PubMed articles for VPS18 gene (see all 15
):- Molecular cloning and characterization of human VPS18, VPS11, VPS16, and VPS33. (PubMed id 11250079)2, 3, 4 Huizing M.... Kraemer H. (2001)
- Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase. (PubMed id 16203730)1, 2, 3 Yogosawa S....Akazawa C. (2005)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)3, 4 Olsen J.V....Mann M. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Molecular characterization of mammalian homologues of class c Vps proteins that interact with syntaxin-7. (PubMed id 11382755)3, 4 Kim B.Y....Akazawa C. (2001)
- Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10819331)3, 4 Nagase T....Ohara O. (2000)
- Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability. (PubMed id 16996030)1, 3 Yogosawa S....Akazawa C. (2006)
- Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. (PubMed id 18691976)4 Daub H.... Mann M. (2008)
- A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)4 Dephoure N.... Gygi S.P. (2008)
- Large-scale mapping of human protein-protein interactions by mass spectrometry. (PubMed id 17353931)3 Ewing R.M....Figeys D. (2007)
|
Search for VPS18
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing VPS18
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing VPS18
(According to HUGE)
About This Section
| |
Specialized Databases showing VPS18(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for VPS18(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for VPS18:

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