Free for academic non-profit institutions. Other users need a Commercial license

Aliases for VPRBP Gene

Aliases for VPRBP Gene

  • Vpr (HIV-1) Binding Protein 2 3
  • Serine/Threonine-Protein Kinase VPRBP 3 4
  • DDB1- And CUL4-Associated Factor 1 3 4
  • DDB1 And CUL4 Associated Factor 1 2 3
  • HIV-1 Vpr-Binding Protein 3 4
  • Vpr-Interacting Protein 3 4
  • DCAF1 3 4
  • Vpr-Binding Protein 3
  • Protein VPRBP 3
  • EC 2.7.11.1 4
  • KIAA0800 4
  • VprBP 4
  • RIP 4

External Ids for VPRBP Gene

Previous GeneCards Identifiers for VPRBP Gene

  • GC03M051409

Summaries for VPRBP Gene

GeneCards Summary for VPRBP Gene

VPRBP (Vpr (HIV-1) Binding Protein) is a Protein Coding gene. Diseases associated with VPRBP include hiv-1. Among its related pathways are Class I MHC mediated antigen processing and presentation and Class I MHC mediated antigen processing and presentation.

UniProtKB/Swiss-Prot for VPRBP Gene

  • Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of Thr-120 of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714). In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. The HIV-1 Vpr protein hijacks the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex to promote ubiquitination and degradation of proteins such as TERT and ZIP/ZGPAT. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage.

Gene Wiki entry for VPRBP Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for VPRBP Gene

Genomics for VPRBP Gene

Regulatory Elements for VPRBP Gene

Genomic Location for VPRBP Gene

Start:
51,395,867 bp from pter
End:
51,505,611 bp from pter
Size:
109,745 bases
Orientation:
Minus strand

Genomic View for VPRBP Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for VPRBP Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for VPRBP Gene

Proteins for VPRBP Gene

  • Protein details for VPRBP Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y4B6-VPRBP_HUMAN
    Recommended name:
    Protein VPRBP
    Protein Accession:
    Q9Y4B6
    Secondary Accessions:
    • Q2YD74
    • Q8TBD9
    • Q9HCA1
    • Q9UG37

    Protein attributes for VPRBP Gene

    Size:
    1507 amino acids
    Molecular mass:
    169007 Da
    Quaternary structure:
    • Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex. Interacts with DDB1; the interaction is direct. Also forms a ternary complex with DDA1 and DDB1. Interacts with NF2 (via FERM domain). Component of the EDVP complex, a E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP. Interacts with DYRK2; the interaction is direct. Interacts with RAG1; the interaction is direct. Interacts with LLGL1 and LLGL2. Interacts with histone H3. Interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein; the interaction is direct.
    SequenceCaution:
    • Sequence=BAA34520.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for VPRBP Gene

    Alternative splice isoforms for VPRBP Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for VPRBP Gene

Proteomics data for VPRBP Gene at MOPED

Post-translational modifications for VPRBP Gene

  • Ubiquitination at Lys280 and Lys1224
  • Modification sites at PhosphoSitePlus

Other Protein References for VPRBP Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for VPRBP Gene

Domains for VPRBP Gene

Gene Families for VPRBP Gene

HGNC:
  • DCAF :DDB1 and CUL4 associated factors

Suggested Antigen Peptide Sequences for VPRBP Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y4B6

UniProtKB/Swiss-Prot:

VPRBP_HUMAN :
  • Q9Y4B6
Domain:
  • The protein kinase-like region mediates the threonine-protein kinase activity.
  • The DWD boxes are required for interaction with DDB1
  • The chromo domain with a restricted pocket directly recognizes monomethylated substrates.
  • Contains 1 chromo domain.
  • Contains 1 LisH domain.
Family:
  • Belongs to the VPRBP/DCAF1 family.
Similarity:
  • Contains 5 WD repeats.
genes like me logo Genes that share domains with VPRBP: view

Function for VPRBP Gene

Molecular function for VPRBP Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot Function: Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of Thr-120 of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714). In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. The HIV-1 Vpr protein hijacks the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex to promote ubiquitination and degradation of proteins such as TERT and ZIP/ZGPAT. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage.
UniProtKB/Swiss-Prot Induction: Up-regulated in a number of cancer cell lines (at protein level).

Enzyme Numbers (IUBMB) for VPRBP Gene

Gene Ontology (GO) - Molecular Function for VPRBP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005488 binding --
GO:0005515 protein binding IPI 17314515
GO:0005524 ATP binding IEA --
GO:1990244 histone kinase activity (H2A-T120 specific) IDA 24140421
genes like me logo Genes that share ontologies with VPRBP: view
genes like me logo Genes that share phenotypes with VPRBP: view

Animal Models for VPRBP Gene

MGI Knock Outs for VPRBP:

miRNA for VPRBP Gene

miRTarBase miRNAs that target VPRBP

No data available for Transcription Factor Targeting and HOMER Transcription for VPRBP Gene

Localization for VPRBP Gene

Subcellular locations from UniProtKB/Swiss-Prot for VPRBP Gene

Cytoplasm. Nucleus. Note=Associated with chromatin in a DDB1-independent and cell cycle-dependent manner: recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for VPRBP Gene COMPARTMENTS Subcellular localization image for VPRBP gene
Compartment Confidence
nucleus 5
cytosol 1
endoplasmic reticulum 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for VPRBP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IEA --
GO:0008180 colocalizes_with COP9 signalosome IDA 18850735
genes like me logo Genes that share ontologies with VPRBP: view

Pathways for VPRBP Gene

genes like me logo Genes that share pathways with VPRBP: view

UniProtKB/Swiss-Prot Q9Y4B6-VPRBP_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for VPRBP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA 24140421
GO:0006351 transcription, DNA-templated IEA --
GO:0016032 viral process IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030183 B cell differentiation ISS --
genes like me logo Genes that share ontologies with VPRBP: view

Transcripts for VPRBP Gene

Unigene Clusters for VPRBP Gene

Vpr (HIV-1) binding protein:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for VPRBP Gene

No ASD Table

Relevant External Links for VPRBP Gene

GeneLoc Exon Structure for
VPRBP
ECgene alternative splicing isoforms for
VPRBP

Expression for VPRBP Gene

mRNA expression in normal human tissues for VPRBP Gene

mRNA differential expression in normal tissues according to GTEx for VPRBP Gene

This gene is overexpressed in Testis (5.8).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for VPRBP Gene

SOURCE GeneReport for Unigene cluster for VPRBP Gene Hs.716623

mRNA Expression by UniProt/SwissProt for VPRBP Gene

Q9Y4B6-VPRBP_HUMAN
Tissue specificity: Ubiquitously expressed.
genes like me logo Genes that share expressions with VPRBP: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for VPRBP Gene

Orthologs for VPRBP Gene

This gene was present in the common ancestor of animals.

Orthologs for VPRBP Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia VPRBP 35
  • 99.8 (n)
  • 100 (a)
VPRBP 36
  • 79 (a)
OneToOne
cow
(Bos Taurus)
Mammalia VPRBP 35
  • 91.97 (n)
  • 98.27 (a)
VPRBP 36
  • 71 (a)
OneToOne
dog
(Canis familiaris)
Mammalia VPRBP 35
  • 94.09 (n)
  • 98.74 (a)
VPRBP 36
  • 71 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Vprbp 35
  • 91.97 (n)
  • 98.28 (a)
Vprbp 16
Vprbp 36
  • 68 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia VPRBP 36
  • 70 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 76 (a)
OneToMany
-- 36
  • 32 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Vprbp 35
  • 92.37 (n)
  • 98.64 (a)
chicken
(Gallus gallus)
Aves VPRBP 35
  • 81.95 (n)
  • 93.54 (a)
VPRBP 36
  • 68 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia VPRBP 36
  • 67 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia vprbp 35
  • 74.75 (n)
  • 85.74 (a)
zebrafish
(Danio rerio)
Actinopterygii VPRBP 36
  • 81 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012134 35
  • 51.14 (n)
  • 45.89 (a)
fruit fly
(Drosophila melanogaster)
Insecta mahj 35
  • 48.86 (n)
  • 42.42 (a)
mahj 36
  • 26 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea dcaf-1 35
  • 46.98 (n)
  • 37.74 (a)
dcaf-1 36
  • 20 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 29 (a)
OneToOne
Species with no ortholog for VPRBP:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for VPRBP Gene

ENSEMBL:
Gene Tree for VPRBP (if available)
TreeFam:
Gene Tree for VPRBP (if available)

Paralogs for VPRBP Gene

No data available for Paralogs for VPRBP Gene

Variants for VPRBP Gene

Sequence variations from dbSNP and Humsavar for VPRBP Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs1898302 -- 51,444,876(+) ttcac(C/T)gtgtt intron-variant
rs2033703 -- 51,467,395(-) ctcgc(A/G)atagt intron-variant
rs2082992 -- 51,491,078(-) gccac(C/T)gcgcc intron-variant
rs2082993 -- 51,491,055(-) tgttt(G/T)ttttt intron-variant
rs2118135 -- 51,447,714(+) aggcc(A/G)aggcg intron-variant

Structural Variations from Database of Genomic Variants (DGV) for VPRBP Gene

Variant ID Type Subtype PubMed ID
nsv3816 CNV Insertion 18451855
esv2646677 CNV Deletion 19546169
esv1010319 CNV Insertion 20482838
nsv508920 CNV Insertion 20534489
esv992244 CNV Insertion 20482838

Relevant External Links for VPRBP Gene

HapMap Linkage Disequilibrium report
VPRBP

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for VPRBP Gene

Disorders for VPRBP Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for VPRBP Gene including...

Search for VPRBP Gene in MalaCards »

Relevant External Links for VPRBP

Genetic Association Database (GAD)
VPRBP
Human Genome Epidemiology (HuGE) Navigator
VPRBP
genes like me logo Genes that share disorders with VPRBP: view

No data available for UniProtKB/Swiss-Prot for VPRBP Gene

Publications for VPRBP Gene

  1. Cytoplasmic retention of HIV-1 regulatory protein Vpr by protein- protein interaction with a novel human cytoplasmic protein VprBP. (PMID: 11223251) Zhang S. … Zhao L.-J. (Gene 2001) 2 3 4 23
  2. Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. (PMID: 18606781) McCall C.M. … Xiong Y. (Mol. Cell. Biol. 2008) 3 4 23
  3. Biochemical mechanism of HIV-I Vpr function. Specific interaction with a cellular protein. (PMID: 8195203) Zhao L.-J. … Narayan O. (J. Biol. Chem. 1994) 2 3 4
  4. The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A- DDB1 DCAF1 ubiquitin ligase to overcome a postentry block in macrophage infection. (PMID: 19264781) Bergamaschi A. … Transy C. (J. Virol. 2009) 3 4
  5. VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation. (PMID: 18332868) Huang J. … Chen J. (Oncogene 2008) 3 4

Products for VPRBP Gene

Sources for VPRBP Gene

Back to Top

Content