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VIL1 Gene

protein-coding   GIFtS: 60

GC02P218992
villin 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: VIL)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
D2S1471 1, 2
OTTHUMP00000164145 2
VIL 2, 3
Descriptions
villin 1 2
External Ids
HGNC: 126901
Entrez Gene: 74292
UniProtKB: P093273
Ensembl: ENSG000001278317
Search outside databases for aliases for VIL1 gene

Previous GC identifers: GC02P217304 GC02P218009 GC02P219247 GC02P219491 GC02P219113

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for VIL1:
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein
represents a dominant part of the brush border cytoskeleton which functions in the capping,
severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they
result from utilization of alternate poly-adenylation signals present in the terminal exon.
[provided by RefSeq]

UniProtKB/Swiss-Prot: VILI_HUMAN, P09327
Function: Ca(2+)-regulated actin-binding protein

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VIL1 gene  

Entrez Gene cytogenetic band: 2q35-q36   Ensembl cytogenetic band:  2q35   HGNC cytogenetic band: 2q35

VIL1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P218992:     (about GC identifiers)

Start:
218,992,089 bp from pter
End:
219,026,262 bp from pter
Size:
34,174 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: VILI_HUMAN, P09327 (See protein sequence)
Recommended Name: Villin-1  
Size: 827 amino acids; 92695 Da
Subunit: Monomer
Subcellular location: Cytoplasm, cytoskeleton
PDB structures from and Proteopedia :
1UNC (3D)    
Secondary accessions: B2R9A7

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_009058.2  

    ENSEMBL proteins: 
    ENSP00000248444 ENSP00000375962 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Villin)
    Human Recombinant Proteins from Abnova (VIL1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA18029348
    GO:0005737 cytoplasm IEA--
    GO:0005856 cytoskeleton IEA--
    About this table

    Antibodies for VIL1: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of VIL1
    Sigma-Aldrich Antibodies for VIL1
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for VIL1  (Villin-1)
    Antibodies from Abcam (Villin), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (VIL1)
    Novus Biologicals Antibodies for VIL1

    Assays for VIL1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR015627 Villin
     IPR007122 Gelsolin
     IPR003128 Villin_headpiece
     IPR007123 Gelsoln


       GeneDecks  VIL1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P09327

    ProtoNet protein and cluster: P09327

    3 Blocks protein families:
    IPB003128 Villin headpiece
    IPB007122 Gelsolin family signature
    IPB007123 Gelsolin region


    UniProtKB/Swiss-Prot: VILI_HUMAN, P09327
    Domain: Consists of a large core fragment, the N-terminal portion, and a small headpiece, the
    C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of
    calcium
    Similarity: Belongs to the villin/gelsolin family
    Similarity: Contains 6 gelsolin-like repeats
    Similarity: Contains 1 HP (headpiece) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VIL1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VIL1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): BC017303

                  Applied Biosystems Silencer® siRNAs for VIL1

                  Sigma-Aldrich siRNA for VIL1  
                         Sigma-Aldrich shRNA for VIL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for VIL1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007127
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007127
                                     untagged cDNA clones in CMV expression vector (see all 2): BC017303 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_007127

    UniProtKB/Swiss-Prot: VILI_HUMAN, P09327
    Function: Ca(2+)-regulated actin-binding protein

    Genatlas biochemistry entry for VIL1:
    villin 1 (95kDa),component of intestinal brush border cytoskeleton,actin binding protein

    2 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Vil1):

    homeostasis/metabolismnormal

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779 actin binding IEA--
    GO:0005509 calcium ion binding IEA--
    GO:0005515 protein binding IPI17353931
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  VIL1 


    5/15 Interacting proteins for VIL1 (ENSP000002484443 P093271, 2) via UniProtKB, MINT, and/or STRING (see all 15 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PPM1BO756881EBI-1047253, EBI-1047039
    TNP1ENSP000002369793STRING (score=.943)
    IL10RAENSP000002277523STRING (score=.936)
    SLC11A1ENSP000002332023STRING (score=.89)
    IL10ENSP000003560663STRING (score=.819)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006461 protein complex assembly TAS2846586
    GO:0007010 cytoskeleton organization IEA--
    GO:0051014 actin filament severing TAS2846586
    GO:0051016 barbed-end actin filament capping IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for VIL1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VIL1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VIL1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): BC017303

                  Sigma-Aldrich siRNA for VIL1  
                         Sigma-Aldrich shRNA for VIL1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_007127  

    REFSEQ mRNAs for VIL1 gene: 

    NM_007127.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_007127  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007127
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007127
                                     untagged cDNA clones in CMV expression vector (see all 2): BC017303 

    Additional cDNA sequence: 

    AK223398.1 AK300965.1 AK313709.1 BC017303.2 X04657.1 X12901.1 

    8 DOTS entries:

    DT.86839119  DT.416827  DT.95360700  DT.92429189  DT.95366402  DT.100788777  DT.40106351  DT.102823347 

    24/125 AceView cDNA sequences (see all 125 ):

    BX505818 AI910841 BX483099 AI355117 AA502293 BM852368 AW139085 AV697342 
    CA429743 BM823478 AA128234 AA887782 AI304462 BE502338 AA305307 BE463508 
    AW444781 BF197572 BC017303 AI699588 N74218 AA876039 AA527320 AW273255 

    highest scoring ESTs for VIL1:

    X12901 AA527320 AI346370 AI355117 AI433336 AV697342 AW242356 AW269183 AW951431 BF063361 

    Unigene Cluster for VIL1:

    Villin 1
    Hs.654595  [show with all ESTs]
    Unigene Representative Sequence: NM_007127


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for VIL1

    ExUns: 1 ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18 ^ 19 ^ 20
    SP1:                                                                          -                 -                                                         
    SP2:                                                                                                                                                      
    SP3:                    -     -     -     -                                                                                                               
    SP4:                                                                                                                                                      
    SP5:                          -                                                                                                                           

    About this scheme

    ECgene alternative splicing isoforms for VIL1

    2 Ensembl transcripts including schematic representations:
    ENST00000248444  ENST00000392114  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    VIL1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for VIL1

    1 / 2 / 3

    10 probe-sets matching VIL1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    51129_s_at2, 3 U95-C 1 1.00 1.00 0.93 0.98 AI436136 0.20 0.50 0.38 1

    61664_r_at2, 3 U95-C 1 0.94 1.00 0.72 1.07 AI859398 0.40 1.00 0.76 1
    35415_at2, 3 U95-A 1 0.94 1.00 0.95 0.86 X12901 1.00 1.00 1.00 1

    62627_r_at2, 3 U95-C 2 0.88 0.96 0.73 1.37 AI765901 0.20 0.33 0.27 2

    228912_at2, 3 U133-B 1 1.00 1.00 -- -- AI436136 0.20 0.50 0.38 1

    205506_at2, 3 U133-A 1 1.00 1.00 -- -- NM_007127 0.60 1.00 0.82 1

    1554943_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228912_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    205506_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1554945_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  VIL1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CCAACGAGGA

    SOURCE GeneReport for Unigene cluster: Hs.654595

    Expression variation in blood from EXPOLDB for VIL1

    UniProtKB/Swiss-Prot: VILI_HUMAN, P09327
    Tissue specificity: Major component of microvilli of intestinal epithelial cells and kidney
    proximal tubule cells

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for VIL1 gene from 5/12 species (see all 12 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    VIL11   -- villin 1 87.34(n)
    88.39(a)
    488522  XM_545642.2  XP_545642.2 
    chimpanzee
    (Pan troglodytes)
    VIL11   -- villin 1 99.27(n)
    99.4(a)
    470646  XM_526028.2  XP_526028.2 
    cow
    (Bos taurus)
    VIL11   -- villin 1 88.92(n)
    89.48(a)
    281573  NM_001013591.1  NP_001013609.1 
    rat
    (Rattus norvegicus)
    Vil11   -- villin 1 87.06(n)
    89.72(a)
    316521  XM_237288.4  XP_237288.3 
    mouse
    (Mus musculus)
    Vil11, 5 1 (40.80 cM)5
    villin 11, 5 86.54(n)1
    89.84(a)1
    223491  NM_009509.11  NP_033535.11 
     AI6634565  AK0279085  (see all 11)
    About this table        Species with no ortholog for VIL1

    ENSEMBL Gene Tree for VIL1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for VIL1 gene
    AVIL2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/127 NCBI SNPs in VIL1 are shown (see all 127 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 43)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs67174341,2
    A,C,F,H218996381(+) gaaaaG/AaaTAA 1 -- ng51 trp34Minor allele frequency- A:0.06EU EA WA 420
    rs174626301,2
    C,F,H218994785(+) CTCTTG/CCTGCC 1 -- ng5111Minor allele frequency- C:0.12NA EU EA WA 978
    rs101657541,2
    C,F,H218995614(+) AAGTCA/GCTAAG 1 -- ng5112Minor allele frequency- N:0.00EU EA WA NA 1046
    rs101894791,2
    C,F,H218995520(+) CTGGGC/AAGGGC 1 -- ng517Minor allele frequency- A:0.40NA EU EA WA 548
    rs353055401,2
    F219002445(+) ACTCAA/GACTGT 1 K/R mis11Minor allele frequency- G:0.03NA 72
    rs46728931,2
    C218996626(+) CCCCAA/GGCCCC 1 -- ng510--------
    --
    rs115592891,2
    C219000968(+) AAGCAC/TGTGGA 1 H/H syn1 ese30--------
    rs46743051,2
    A,C,F,H219001570(+) ACATAT/CGTGTG 1 -- int116Minor allele frequency- C:0.49NS EU EA WA NA 1084
    rs67204031,2
    A,C,F,H219002502(+) GGGAGA/CAGGTG 1 -- int13Minor allele frequency- C:0.17EU EA 256
    rs46743081,2
    A,C,F,H219018727(+) AGATGC/ATGTTT 1 -- int18Minor allele frequency- A:0.20EU EA WA 810
    About this table

    HapMap Linkage Disequilibrium images for VIL1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 193040

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/33 PubMed articles for VIL1 gene (see all 33 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7429 HGNC: 12690 AceView: VIL1 Ensembl:ENSG00000127831 euGenes: HUgn7429
    ECgene: VIL1 H-InvDB: VIL1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for VIL1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for VIL1 Antibodies for VIL1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for VIL1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (Villin)
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     Search OriGene for VIL1
     Search Tocris compounds for VIL1




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