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VCPIP1 Gene

protein-coding   GIFtS: 54

GC08M067706
valosin containing protein (p97)/p47 complex interacting protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DKFZp686G038 2
DUBA3 1, 2
EC 3.4.22.- 3
FLJ23132 1, 2
FLJ60694 2
KIAA1850 1, 2, 3, 5
VCIP135 1, 2, 3
Descriptions
VCPIP1 VCIP135 5
Valosin-containing protein p97/p47 complex-interacting
protein 1 3
Valosin-containing protein p97/p47 complex-interacting
protein p135 3
valosin containing protein (p97)/p47 complex interacting
protein 1 2
valosin-containing protein (p97)/p47 complex-interacting
protein p135 2
External Ids
HGNC: 308971
Entrez Gene: 801242
UniProtKB: Q96JH73
Ensembl: ENSG000001750737
Search outside databases for aliases for VCPIP1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: VCIP1_HUMAN, Q96JH7
Function: Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks
after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum
(tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By
similarity)

Gene Wiki entry for VCPIP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VCPIP1 gene  

Entrez Gene cytogenetic band: 8q13   Ensembl cytogenetic band:  8q13.1   HGNC cytogenetic band: 8q13

VCPIP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M067706:     (about GC identifiers)

Start:
67,703,300 bp from pter
End:
67,742,036 bp from pter
Size:
38,737 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_008183.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: VCIP1_HUMAN, Q96JH7 (See protein sequence)
Recommended Name: Deubiquitinating protein VCIP135  
Size: 1222 amino acids; 134321 Da
Subunit: Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP (By similarity)
Subcellular location: Endoplasmic reticulum (By similarity). Golgi apparatus, Golgi stack (By
similarity). Note=Associated with Golgi stacks and endoplasmic reticulum (By similarity)
Sequence caution: Sequence=BAB15552.1; Type=Frameshift; Positions=1065, 1110;
Secondary accessions: Q504T4 Q86T93 Q86W01 Q8N3A9 Q9H5R8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_079330.2  

    ENSEMBL proteins: 
    ENSP00000309031 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (VCPIP1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IDA11256614
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005794 Golgi apparatus IEA--
    About this table

    Antibodies for VCPIP1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Monoclonal and Polyclonal Antibodies from Abnova (VCPIP1)
    Novus Biologicals Antibody for VCPIP1

    Assays for VCPIP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR003323 OTU


       GeneDecks  VCPIP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q96JH7

    ProtoNet protein and cluster: Q96JH7

    1 Blocks protein family: IPB003323 OTU-like cysteine protease

    UniProtKB/Swiss-Prot: VCIP1_HUMAN, Q96JH7
    Similarity: Contains 1 OTU domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VCPIP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VCPIP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_025054

                  Applied Biosystems Silencer® siRNAs for VCPIP1

                  Sigma-Aldrich siRNA for VCPIP1  
                         Sigma-Aldrich shRNA for VCPIP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_025054
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_025054
                                     untagged cDNA clone in CMV expression vector: NM_025054 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_025054

    UniProtKB/Swiss-Prot: VCIP1_HUMAN, Q96JH7
    Function: Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks
    after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum
    (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By
    similarity)
    Enzyme Number (IUBMB): EC 3.4.22.- 

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004843 ubiquitin-specific protease activity ISS--
    GO:0008233 peptidase activity IEA--
    GO:0008234 cysteine-type peptidase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/8 Interacting proteins for VCPIP1 (ENSP000003090313 Q96JH72) via UniProtKB, MINT, and/or STRING (see all 8 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SMAD9O151982MINT-62103
    VCPENSP000003517773STRING (score=.965)
    PSMD2ENSP000003101293STRING (score=.947)
    STX5ENSP000003671293STRING (score=.938)
    BET1LENSP000003722103STRING (score=.933)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016567 protein ubiquitination ISS--
    GO:0019941 modification-dependent protein catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for VCPIP1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VCPIP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VCPIP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_025054

                  Sigma-Aldrich siRNA for VCPIP1  
                         Sigma-Aldrich shRNA for VCPIP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_025054  

    REFSEQ mRNAs for VCPIP1 gene: 

    NM_025054.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_025054  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_025054
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_025054
                                     untagged cDNA clone in CMV expression vector: NM_025054 

    Additional cDNA sequence: 

    AB058753.1 AF088033.1 AK026785.1 AK225580.1 AK297345.1 AL832606.2 AL834476.1 BC012498.1 
    BC049379.1 BC062426.1 BC094799.1 BC118917.1 CR593204.1 CR936659.1 

    4 DOTS entries:

    DT.40308443  DT.100793230  DT.428776  DT.92352710 

    2 AceView cDNA sequences:

    BF910941 BF377808 

    highest scoring ESTs for VCPIP1:

    AA405241 AB058753 AK026785 AL708282 AL712343 AL832606 AL834476 BC012498 BC049379 BC062426 

    Unigene Cluster for VCPIP1:

    Valosin containing protein (p97)/p47 complex interacting protein 1
    Hs.632066  [show with all ESTs]
    Unigene Representative Sequence: CR936659


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000310421  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    VCPIP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for VCPIP1

    1 / 2 / 3

    8 probe-sets matching VCPIP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    60001_at2, 3 U95-C 1 1.00 1.00 0.95 1.09 AA405241 0.80 1.00 0.91 1
    42761_at2, 3 U95-B 1 1.00 1.00 0.95 0.91 W45259 0.40 1.00 0.76 1

    234988_at2, 3 U133-B 1 1.00 1.00 -- -- AW629515 0.60 1.00 0.82 1

    219810_at2, 3 U133-A 1 1.00 1.00 -- -- NM_025054 0.60 1.00 0.82 1

    234988_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    219810_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1556228_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1556227_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  VCPIP1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTTTTGTAGT

    SOURCE GeneReport for Unigene cluster: Hs.632066
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for VCPIP1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    VCPIP11   -- valosin containing protein (p97)/p47 complex interacting more 95(n)
    97.21(a)
    486980  XM_544109.2  XP_544109.2 
    chimpanzee
    (Pan troglodytes)
    VCPIP11   -- valosin containing protein (p97)/p47 complex interacting more 99.81(n)
    99.92(a)
    472782  XM_001161197.1  XP_001161197.1 
    cow
    (Bos taurus)
    VCPIP11   -- valosin containing protein (p97)/p47 complex interacting more 93.15(n)
    95.9(a)
    539643  XM_588521.3  XP_588521.3 
    rat
    (Rattus norvegicus)
    Vcpip11   -- valosin containing protein (p97)/p47 complex interacting more 91.76(n)
    95.74(a)
    286761  NM_176857.2  NP_789827.2 
    mouse
    (Mus musculus)
    Vcpip11, 5 15
    valosin containing protein (p97)/p47 complex interacting more1, 5 91.69(n)1
    95.57(a)1
    706751  NM_173443.21  NP_775619.21 
     AI1575955  AI5916485  (see all 19)
    About this table        Species with no ortholog for VCPIP1

    ENSEMBL Gene Tree for VCPIP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/143 NCBI SNPs in VCPIP1 are shown (see all 143 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 38)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 8 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs78401921,2
    A,C,F,H67742349(+) GCGTCT/CAGCTG 1 -- ng3111Minor allele frequency- C:0.13EU EA WA NA 976
    rs38242581,2
    C,F,H67705816(-) TTCTAA/CGTTGA 1 -- ut317Minor allele frequency- C:0.03NA EU EA WA 560
    rs29366961,2
    C,F,H67741845(-) TCGCCC/TTGGGC 1 -- ut51 ese37Minor allele frequency- T:0.00EU EA WA NA 562
    rs22771291,2
    F,H67742231(+) TGACTC/GGCGTT 1 -- ng314Minor allele frequency- G:0.06EA WA 1794
    rs169329961,2
    F,H67708760(+) CTCTAT/CGAATG 1 -- ut31 ese37Minor allele frequency- C:0.08NA EU EA WA 560
    rs132709791,2
    H67743251(+) TTACCA/TAAAAA 1 -- ng314Minor allele frequency- T:0.00EU EA WA 420
    rs29568031,2
    H67742310(-) TCTCAG/AACTCT 1 -- ng314Minor allele frequency- A:0.00EU EA WA 420
    rs38086021,2
    A,C,F,H67740224(+) AAGCTG/ATAATT 1 Y/Y syn1 ese319Minor allele frequency- A:0.13NS NA EU EA WA 1354
    rs70035481,2
    A,C,F,H67732179(+) CTAGAC/GAGAAT 1 -- int17Minor allele frequency- G:0.13EU EA WA NA 560
    rs29545641,2
    C,F,H,O67730050(+) acagaT/Gggcaa 1 -- int17Minor allele frequency- G:0.00EU EA WA NA 560
    About this table

    HapMap Linkage Disequilibrium images for VCPIP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611745

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/19 PubMed articles for VCPIP1 gene (see all 19 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 80124 HGNC: 30897 AceView: VCIP135 Ensembl:ENSG00000175073 euGenes: HUgn80124
    ECgene: VCPIP1 H-InvDB: VCPIP1
    (According to HUGE)
    About This Section

    HUGE: KIAA1850
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for VCPIP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for VCPIP1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for VCPIP1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for VCPIP1

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