V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

VBP1 Gene

protein-coding   GIFtS: 60

GC0XP154097
von Hippel-Lindau binding protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
HIBBJ46 3
OTTHUMP00000024252 2
PFD3 1, 2
PFDN3 1, 2, 3
VBP-1 2, 3
Descriptions
VHL binding protein-1 2
VHL-binding protein 1 3
Von Hippel-Lindau-binding protein 1 3
prefoldin 3 2
von Hippel-Lindau binding protein 1 2
External Ids
HGNC: 126621
Entrez Gene: 74112
UniProtKB: P617583
Ensembl: ENSG000001559597
Search outside databases for aliases for VBP1 gene

Previous GC identifers: GC0XM148650 GC0XM150967 GC0XP151961 GC0XP152866 GC0XP154008

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for VBP1:
The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an
intracellular complex. Because it functions as a chaperone protein, it is suspected that it may
play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the
nucleus or cytoplasm. [provided by RefSeq]

UniProtKB/Swiss-Prot: PFD3_HUMAN, P61758
Function: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it.
Binds to nascent polypeptide chain and promotes folding in an environment in which there are many
competing pathways for nonnative proteins

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VBP1 gene  

Entrez Gene cytogenetic band: Xq28   Ensembl cytogenetic band:  Xq28   HGNC cytogenetic band: Xq28

VBP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP154097:     (about GC identifiers)

Start:
154,097,744 bp from pter
End:
154,121,292 bp from pter
Size:
23,549 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011726.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: PFD3_HUMAN, P61758 (See protein sequence)
Recommended Name: Prefoldin subunit 3  
Size: 197 amino acids; 22658 Da
Subunit: Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to the
C-terminal part of VHL
Subcellular location: Cytoplasm. Nucleus. Note=In complex with VHL can translocate to the nucleus
Secondary accessions: B2R8L5 O55228 Q15765 Q5JT81 Q86X96

REFSEQ proteins: NP_003363.1  

ENSEMBL proteins: 
ENSP00000286428 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (PFDN3)
Human Recombinant Proteins from Abnova (VBP1)
              OriGene Purified Recombinant Human Protein: VBP1 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
GO:0005737 cytoplasm TAS8674032
GO:0016272 prefoldin complex IEA--
About this table

Antibodies for VBP1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for VBP1
Browse R&D Systems for Antibodies
Antibodies from Abcam (PFDN3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (VBP1)
Novus Biologicals Antibodies for VBP1

Assays for VBP1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR004127 PFD_alpha-like
 IPR016655 Prefoldin_su-3


   GeneDecks  VBP1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry P61758

ProtoNet protein and cluster: P61758

UniProtKB/Swiss-Prot: PFD3_HUMAN, P61758
Similarity: Belongs to the prefoldin subunit alpha family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (VBP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (VBP1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003372

              Applied Biosystems Silencer® siRNAs for VBP1

              Sigma-Aldrich siRNA for VBP1  
                     Sigma-Aldrich shRNA for VBP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for VBP1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003372
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003372
                                 untagged cDNA clone in CMV expression vector: NM_003372 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_003372

UniProtKB/Swiss-Prot: PFD3_HUMAN, P61758
Function: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it.
Binds to nascent polypeptide chain and promotes folding in an environment in which there are many
competing pathways for nonnative proteins

Genatlas biochemistry entry for VBP1:
von Hippel-Lindau binding protein 1,unlikely to oinvolved in renal cell carcinoma

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0051082 unfolded protein binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  VBP1 


5/31 Interacting proteins for VBP1 (P617582 ENSP000002864283) via UniProtKB, MINT, and/or STRING (see all 31 )
InteractantInteraction Details
GeneCardExternal ID(s)
MAP3K3Q997592MINT-48489
PFDN5ENSP000003341883STRING (score=.982)
PFDN2ENSP000003569893STRING (score=.98)
PFDN4ENSP000003604733STRING (score=.98)
PFDN1ENSP000002618133STRING (score=.962)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006457 protein folding IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for VBP1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (VBP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (VBP1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003372

              Sigma-Aldrich siRNA for VBP1  
                     Sigma-Aldrich shRNA for VBP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003372  

REFSEQ mRNAs for VBP1 gene: 

NM_003372.5   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003372  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003372
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003372
                                 untagged cDNA clone in CMV expression vector: NM_003372 

Additional cDNA sequence: 

AK301646.1 AK313420.1 BC009570.1 BC040002.1 BC046094.1 BT019604.1 CR541932.1 CR595254.1 
CR596441.1 CR600706.1 CR604493.1 CR608323.1 CR609299.1 CR611663.1 CR612569.1 CR616139.1 
CR616685.1 CR618821.1 CR623613.1 CR624898.1 CR625553.1 U56833.1 U96759.1 Y17394.1 

12 DOTS entries:

DT.92343706  DT.447718  DT.121318678  DT.100822378  DT.100822382  DT.91814280  DT.91772134  DT.121318671 
DT.91772137  DT.100822381  DT.121318676  DT.91801616 

24/478 AceView cDNA sequences (see all 478 ):

AA373354 F37214 BU628276 CR608323 AI151351 AI015896 AA775189 BM970439 
W38812 CA423632 Z41853 BM459952 AA253223 AI497998 BU077548 AA789143 
BC046094 CR623613 AI301193 BF445589 CB242369 BF940000 F26994 AI753943 

highest scoring ESTs for VBP1:

U56833 AA024631 AA027014 AA039779 AA039780 AA176288 AA181112 AA252912 AA253223 AA373354 

Unigene Cluster for VBP1:

Von Hippel-Lindau binding protein 1
Hs.436803  [show with all ESTs]
Unigene Representative Sequence: U96759


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for VBP1

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7
SP1:                    -           -                                       
SP2:                    -           -                                       
SP3:                                -                             -         
SP4:                                                                        
SP5:                                -                                       

About this scheme

ECgene alternative splicing isoforms for VBP1

1 Ensembl transcript including schematic representation:
ENST00000286428  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

VBP1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for VBP1

1 / 2 / 3

3 probe-sets matching VBP1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
171_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 U56833 1.00 1.00 1.00 1

201472_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003372 0.60 1.00 0.82 1

201472_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  VBP1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AAATTAAAAC

SOURCE GeneReport for Unigene cluster: Hs.436803

Expression variation in blood from EXPOLDB for VBP1

UniProtKB/Swiss-Prot: PFD3_HUMAN, P61758
Tissue specificity: Ubiquitous

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for VBP1 gene from 5/21 species (see all 21 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
VBP11   -- von Hippel-Lindau binding protein 1 94.42(n)
96.45(a)
481089  XM_538210.2  XP_538210.1 
chimpanzee
(Pan troglodytes)
VBP11   -- von Hippel-Lindau binding protein 1 97.29(n)
95.93(a)
465952  XM_521348.2  XP_521348.2 
cow
(Bos taurus)
VBP11   -- von Hippel-Lindau binding protein 1 91.37(n)
94.92(a)
508727  NM_001038516.1  NP_001033605.1 
mouse
(Mus musculus)
Vbp11, 5 X (30.80 cM)5
von Hippel-Lindau binding protein 11, 5 91.84(n)1
97.45(a)1
223271  NM_011692.21  NP_035822.21 
 AI3259405  AK0022825  (see all 24)
chicken
(Gallus gallus)
VBP11   -- von Hippel-Lindau binding protein 1 81.73(n)
89.34(a)
422355  XM_420327.2  XP_420327.1 
About this table        Species with no ortholog for VBP1

ENSEMBL Gene Tree for VBP1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/69 NCBI SNPs in VBP1 are shown (see all 69 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 36)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr X posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs118871,2
A,C,F,H,O154120651(-) CAGCCG/AATTAG 1 -- ut31 ese310Minor allele frequency- A:0.44MN EA NA EU WA 977
rs35991,2
C,F,H154120931(-) TTGCAA/GAGACA 1 -- ut314Minor allele frequency- G:0.04EU EA WA 418
rs5720131,2
C,H154109941(-) ACACAC/TTTTAT 1 V/M mis19Minor allele frequency- T:0.00EA NA EU WA 646
--
rs173282151,2
F154120587(+) AAAACT/CGTGAG 1 -- ut313Minor allele frequency- C:0.01NA 112
rs59837021,2
A,C,F,H154112836(-) CAATCG/AGAACT 1 -- int18Minor allele frequency- A:0.04EU EA WA 628
rs123536761,2
A,C,F,H154111116(+) tctttC/Tgtaac 1 -- int18Minor allele frequency- T:0.04EU EA WA 626
rs5330021,2
A,C,F,H154113381(-) CTATAC/TGTGCA 1 -- int18Minor allele frequency- T:0.03EU EA WA 626
rs4802101,2
A,C,F,H154099208(-) ctcgaA/Gttttg 1 -- int17Minor allele frequency- G:0.17NA EA EU WA 670
rs5471141,2
A,C,F,H154118778(-) GGCGAT/CTGATT 1 -- int19Minor allele frequency- C:0.04NA EU EA WA 636
rs59404531,2
C,F,H154098953(-) AGATTG/AAGAAA 1 -- int14Minor allele frequency- A:0.05EU EA WA 418
About this table

HapMap Linkage Disequilibrium images for VBP1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300133

1 Novoseek disease relationship for VBP1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tumors 28.17 2 10441001 (1), 8674032 (1)
About this table

1 PharmGKB disease relationship for VBP1 gene
Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
Leukemia, Lymphocytic, Acute, L1FA  GN  12086872 12730115
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/23 PubMed articles for VBP1 gene (see all 23 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 7411 HGNC: 12662 AceView: VBP1 Ensembl:ENSG00000155959 euGenes: HUgn7411
ECgene: VBP1 H-InvDB: VBP1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for VBP1 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for VBP1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for VBP1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for VBP1 Antibodies for VBP1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for VBP1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Recombinant Proteins (VBP1)
 Antibodies (VBP1)
 Chimera RNAi (VBP1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(PFDN3)
 Antibodies (PFDN3)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for VBP1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for VBP1

GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
VBP1 Gene at Home site.
Version 2.41.1
server5.xennexinc.com