V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

VAT1 Gene

protein-coding   GIFtS: 51

GC17M038420
vesicle amine transport protein 1 homolog (T. californica)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 1.-.-.- 3
FLJ20230 1, 2
VATI 1, 2
Descriptions
membrane protein of cholinergic synaptic vesicles 2
synaptic vesicle membrane protein VAT-1 homolog 2
vesicle amine transport protein 1 2
vesicle amine transport protein 1 homolog (T. californica) 2
External Ids
HGNC: 169191
Entrez Gene: 104932
UniProtKB: Q995363
Ensembl: ENSG000001088287
Search outside databases for aliases for VAT1 gene

Previous GC identifers: GC17P062358 GC17M043328 GC17M041075 GC17M041539

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for VAT1:
Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in
the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of
cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to
the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided
by RefSeq]

Gene Wiki entry for VAT1
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VAT1 gene  

Entrez Gene cytogenetic band: 17q21   Ensembl cytogenetic band:  17q21.31   HGNC cytogenetic band: 17q21

VAT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M038420:     (about GC identifiers)

Start:
38,420,148 bp from pter
End:
38,427,985 bp from pter
Size:
7,838 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000017.9  NT_010755.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: VAT1_HUMAN, Q99536 (See protein sequence)
Recommended Name: Synaptic vesicle membrane protein VAT-1 homolog  
Size: 393 amino acids; 41920 Da
Secondary accessions: Q13035 Q5BKZ7 Q96A39 Q9BUT8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006364.2  

    ENSEMBL proteins: 
    ENSP00000326121 ENSP00000347872 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (VAT1)
                  OriGene Purified Recombinant Human Protein: VAT1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016021 integral to membrane TAS8938427
    About this table

    Antibodies for VAT1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Monoclonal and Polyclonal Antibodies from Abnova (VAT1)
    Novus Biologicals Antibody for VAT1

    Assays for VAT1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR013149 ADH_Zn-bd
     IPR016040 NAD(P)-bd_dom
     IPR013154 ADH_GroES-like
     IPR002364 Quin_OxRdtase/zeta-crystal_CS
     IPR002085 ADH_SF_Zn


       GeneDecks  VAT1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q99536

    ProtoNet protein and cluster: Q99536

    2 Blocks protein families:
    IPB002364 Quinone oxidoreductase/zeta-crystallin
    IPB013154 Alcohol dehydrogenase GroES-like


    UniProtKB/Swiss-Prot: VAT1_HUMAN, Q99536
    Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase
    subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VAT1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VAT1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006373

                  Applied Biosystems Silencer® siRNAs for VAT1

                  Sigma-Aldrich siRNA and siRNA Panels for VAT1  
                         Sigma-Aldrich shRNA for VAT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006373
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006373
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_006373 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006373

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005488 binding IEA--
    GO:0008270 zinc ion binding IEA--
    GO:0016491 oxidoreductase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0055114 oxidation reduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for VAT1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VAT1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VAT1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006373

                  Sigma-Aldrich siRNA and siRNA Panels for VAT1  
                         Sigma-Aldrich shRNA for VAT1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006373  

    REFSEQ mRNAs for VAT1 gene: 

    NM_006373.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006373  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006373
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006373
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_006373 

    Additional cDNA sequence: 

    AK097072.1 AK290460.1 AK298536.1 AK315838.1 BC001913.1 BC008725.2 BC014279.2 BC015041.2 
    BC068489.1 BC090858.1 BT007369.1 CR594124.1 CR613076.1 CR618877.1 CR620205.1 CR622345.1 
    U18009.1 U25779.1 

    24/37 DOTS entries (see all 37 ):

    DT.100848141  DT.453255  DT.100753643  DT.100848135  DT.100848117  DT.100848113  DT.100848128  DT.95122928 
    DT.120905195  DT.100848130  DT.120905128  DT.120905171  DT.100040859  DT.100045675  DT.100848139  DT.120905150 
    DT.100031858  DT.120905142  DT.120905240  DT.92461733  DT.95122766  DT.95122912  DT.95122921  DT.120905136 

    24/1090 AceView cDNA sequences (see all 1090 ):

    AL134862 T35596 BU726332 BM993552 AL134977 AA074307 AU130073 BM708151 
    AU130572 BC008725 BP348955 AA156050 BQ679624 AU130376 BU630544 BQ678035 
    CA421720 BQ218769 BQ678292 CA393756 BQ446235 AI422343 CA406406 BU180924 

    highest scoring ESTs for VAT1:

    U18009 AA334145 AI815606 AL043651 AL516295 AL534921 AL550238 AL550984 AL555940 AL556303 

    Unigene Cluster for VAT1:

    Vesicle amine transport protein 1 homolog (T. californica)
    Hs.514199  [show with all ESTs]
    Unigene Representative Sequence: NM_006373


    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for VAT1 (see all 9 )

    ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b
    SP1:                          -     -                                                                     
    SP2:                                -                                                                     
    SP3:        -     -     -     -     -                                                                     
    SP4:                                                                                                      
    SP5:                                                                                -     -               

    About this scheme

    ECgene alternative splicing isoforms for VAT1

    2 Ensembl transcripts including schematic representations:
    ENST00000315674  ENST00000355653  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    VAT1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for VAT1

    1 / 2 / 3

    6 probe-sets matching VAT1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    48824_at2, 3 U95-B 1 1.00 1.00 0.86 1.41 AW016496 0.80 1.00 0.91 1

    40147_at2, 3 U95-A 1 1.00 1.00 0.90 1.01 U18009 1.00 1.00 1.00 1
    605_at2, 3 U95-A 1 1.00 1.00 0.97 0.94 L78833 0.20 0.25 0.23 1

    625_at2, 3 U95-A 1 1.00 1.00 0.68 0.64 L78833 0.20 0.25 0.23 1

    208626_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC001913 0.80 1.00 0.91 1

    208626_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  VAT1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGTGGGGAGA

    SOURCE GeneReport for Unigene cluster: Hs.514199

    Expression variation in blood from EXPOLDB for VAT1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for VAT1 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    SLC18A11   -- solute carrier family 18 (vesicular monoamine), member more 90.54(n)
    93.61(a)
    490953  XM_548076.2  XP_548076.1 
    chimpanzee
    (Pan troglodytes)
    SLC18A11   -- solute carrier family 18 (vesicular monoamine), member more 99.24(n)
    98.47(a)
    454708  XM_001158563.1  XP_001158563.1 
    cow
    (Bos taurus)
    VAT11   -- vesicle amine transport protein 1 homolog (T. californica) 89.23(n)
    92.62(a)
    510698  XM_587873.3  XP_587873.2 
    rat
    (Rattus norvegicus)
    Vat11   -- vesicle amine transport protein 1 homolog (T californica) 86.67(n)
    91.03(a)
    287721  NM_001033683.1  NP_001028855.1 
    mouse
    (Mus musculus)
    Vat11, 5 115
    vesicle amine transport protein 1 homolog (T californica)1, 5 86.58(n)1
    91.28(a)1
    269491  NM_012037.21  NP_036167.11 
     AI4131695  AK0347955  (see all 30)
    About this table        Species with no ortholog for VAT1

    ENSEMBL Gene Tree for VAT1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for VAT1 gene
    VAT1L2  TP53I32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/67 NCBI SNPs in VAT1 are shown (see all 67 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 36)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs4550551,2
    A,C,F,H38421483(-) CGAAGT/GTCTCT 1 -- ut31 ese38Minor allele frequency- G:0.31MN NA EU EA WA 716
    --
    rs99018501,2
    C,H38421861(+) GAAGGG/CCCAGA 1 A/P mis1 ese34Minor allele frequency- C:0.00EU EA WA 406
    rs10035851,2
    C38420612(-) CTTGCA/GGATAT 1 -- ut31 ese31Minor allele frequency- G:0.00MN 184
    rs4064591,2
    H38421612(-) AGAAGG/CAGAAC 1 Q/E mis1 trp35Minor allele frequency- C:0.00MN EU EA WA 590
    rs20708351,2
    A,C,F38427551(-) CCGCCT/GCTGCC 1 P/P syn1 ese33Minor allele frequency- G:0.34EA MN NA 1572
    --
    rs115396331,2
    H38423631(-) CCCATC/TGACTA 1 I/I syn14Minor allele frequency- T:0.00EU EA WA 406
    rs346625951,2
    F38421856(+) TTCTCG/AAAGGG 1 F/F syn11Minor allele frequency- A:0.04NA 70
    rs3825711,2
    A,C,F,H38425007(+) TGCAGA/GCAAAG 1 -- int18Minor allele frequency- G:0.20EU EA WA 840
    --
    rs99154891,2
    A,C38426752(+) CCCGAA/TGGCTG 1 -- int10--------
    --
    rs99125871,2
    A,C38426612(+) GCGCCA/GCACCT 1 -- int10--------
    About this table

    HapMap Linkage Disequilibrium images for VAT1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604631

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    9 PubMed articles for VAT1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10493 HGNC: 16919 AceView: VAT1 Ensembl:ENSG00000108828 euGenes: HUgn10493
    ECgene: VAT1 H-InvDB: VAT1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for VAT1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for VAT1-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for VAT1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for VAT1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (VAT1)
     Antibodies (VAT1)
     Chimera RNAi (VAT1)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Search for Recombinant Proteins
     Search for Antibodies
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for VAT1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for VAT1

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    VAT1 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com