 |
 | Services
| |
 |
Aliases & Descriptions for VASP
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| | | Descriptions |
|---|
| vasodilator-stimulated phosphoprotein 2 |
|
| | Search outside databases for aliases for VASP genePrevious GC identifers: GC19P046654 GC19P046401 GC19P050686 |
Summaries for VASP(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for VASP: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq] UniProtKB/Swiss-Prot: VASP_HUMAN, P50552Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. VASP promotes actin nucleation and increases the rate of actin polymerization in the presence of capping protein. Plays a role in actin-based activity of Listeria monocytogenes in platelets (By similarity)Gene Wiki entry for VASP (Vasodilator-stimulated_phosphoprotein) |
Genomic Location for VASP
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the VASP gene 
Entrez Gene cytogenetic band: 19q13.2-q13.3 Ensembl cytogenetic band: 19q13.32 HGNC cytogenetic band: 19q13.2-q13.3VASP Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19P050702:
(about GC identifiers)
Start:
|
50,702,528 bp from pter |
End:
|
50,722,081 bp from pter |
Size:
|
19,554 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000019.8 NT_011109.15
| Proteins for VASP
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: VASP_HUMAN, P50552 (See
protein sequence)Recommended Name: Vasodilator-stimulated phosphoprotein Size: 380 amino acids; 39830 Da
Subunit: Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity)
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions
PDB structures from and Proteopedia :1EGX (3D)
 1JNG (3D)
 1USD (3D)
 1USE (3D)
 2PAV (3D)
 2PBD (3D)
 3CHW (3D)
 
Secondary accessions: Q6PIZ1 Q93035Post-translational modifications:
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK11
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_003361.1
ENSEMBL proteins: ENSP00000245932 ENSP00000384386 ENSP00000367017
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: VASP 
5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6
): About this table
Antibodies for VASP: Assays for VASP: | Protein
Domains/ Families for VASP(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P50552
ProtoNet protein and cluster: P50552 UniProtKB/Swiss-Prot: VASP_HUMAN, P50552Domain: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerizationDomain: The WH1 domain mediates interaction with XIRP1Similarity: Belongs to the Ena/VASP familySimilarity: Contains 1 WH1 domain | Gene Function for VASP
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003370
Applied Biosystems Silencer® siRNAs for VASP
Sigma-Aldrich siRNA for VASP  Sigma-Aldrich shRNA Panels and shRNA for VASP  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003370                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003370                                  untagged cDNA clones in CMV expression vector (see all 2): NM_001008736 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_003370
UniProtKB/Swiss-Prot: VASP_HUMAN, P50552Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. VASP promotes actin nucleation and increases the rate of actin polymerization in the presence of capping protein. Plays a role in actin-based activity of Listeria monocytogenes in platelets (By similarity)Genatlas biochemistry entry for VASP:vasodilator-stimulated phosphoprotein,expressed in platelets and a wide variety of other cells,associated with actin filaments and focal adhesions which form the interface between cytoskeleton and extracellular matrix,involved in cGMP and cAMP mediated inhibition of agonist-induced platelet aggregation7 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Vasp):
2 Gene Ontology (GO) molecular function terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003779 | actin binding |
IEA | -- | | GO:0017124 | SH3 domain binding |
IEA | -- | About this table | Pathways & Interactions for VASP
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
5 Sigma-Aldrich "Your Favorite Gene" Pathways for VASP (Your Favorite Gene powered by Ingenuity) 
Gene Network CentralTM Interacting Genes and Proteins Network for VASP 
5/66 Interacting proteins for VASP (P505522 ENSP000002459323) via UniProtKB, MINT, and/or STRING (see all 66
)About this table
|
Drugs & Compounds for VASP(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for VASP 10/13 Novoseek chemical compound relationships for VASP gene (see all 13
)
| Compound |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| prasugrel |
78.51 |
4 |
18217157 (3), 19025429 (1) |
| cgmp |
70.25 |
72 |
12576312 (6), 10710123 (4), 1315268 (3), 18079117 (3) (see all 25) |
| adp |
54.17 |
23 |
18832777 (3), 18022387 (2), 10446085 (2), 17482663 (2) (see all 10) |
| guanylate |
43.92 |
1 |
10710123 (1) |
| forskolin |
42.23 |
4 |
15381390 (1), 14602552 (1), 16973907 (1), 7925440 (1) |
| nitric oxide |
39.42 |
38 |
17049286 (4), 18559661 (4), 15670578 (3), 18445594 (2) (see all 16) |
| proline |
21.96 |
4 |
12529859 (1), 7737110 (1), 14983521 (1) |
| tyrosine |
13.58 |
6 |
9693373 (1), 18983522 (1), 12087107 (1), 16176981 (1) (see all 6) |
| cyclic amp |
11.59 |
2 |
1659381 (1) |
| threonine |
8.32 |
3 |
8182057 (2), 11919161 (1) |
About this table
|
Transcripts for VASP(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003370
Sigma-Aldrich siRNA for VASP  Sigma-Aldrich shRNA Panels and shRNA for VASP  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_003370 REFSEQ mRNAs for VASP gene: NM_003370.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_003370               OriGene GFP tagged cDNA clone in CMV expression vector: NM_003370                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003370                                  untagged cDNA clones in CMV expression vector (see all 2): NM_001008736  Additional cDNA sequence: AK314812.1 BC026019.1 BC038224.1 CR615417.1 Z46389.1 18 DOTS entries: DT.99938233 DT.87017206 DT.100795570 DT.97859810 DT.95258746 DT.100038079 DT.121499781 DT.40305661 DT.75107009 DT.91758247 DT.97859811 DT.100766382 DT.100795577 DT.40118443 DT.95148695 DT.97812970 DT.91691180 DT.95258763 24/341 AceView cDNA sequences (see all 341
):AI540053 BM832076 BQ057786 BM795110 BQ963024 AA748592 AA805727 AL560214 BQ889895 AW002482 AL041942 AI690419 BQ709677 BC026019 AV652596 Z46389 BG745679 BF515553 BC038224 BQ061490 BM893785 BE276838 CR615417 BU855671
highest scoring ESTs for VASP:Z46389 AA034948 AA053271 AA053652 AA128272 AA128569 AA215773 AA251483 AA305874 AA327351 Unigene Cluster for VASP: Vasodilator-stimulated phosphoprotein Hs.515469 [show with all ESTs]Unigene Representative Sequence: NM_003370
GeneLoc Exon Structure
5/9 Alternative Splicing Database (ASD) splice patterns (SP) for VASP (see all 9
)
| ExUns: | 1 | ^ | 2a | · | 2b | · | 2c | ^ | 3a | · | 3b | ^ | 4a | · | 4b | ^ | 5 | ^ | 6 | ^ | 7a | · | 7b | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11a | · | 11b | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15a | · | 15b | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |
| SP5: | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for VASP
3 Ensembl transcripts including schematic representations: ENST00000245932
ENST00000407001
ENST00000377786
|
Expression for VASP
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| VASP expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for VASP
1 / 2 / 3 3 probe-sets matching VASP gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: CTGGCCCGGA
SOURCE GeneReport for Unigene cluster: Hs.515469
Expression variation in blood from EXPOLDB for VASP UniProtKB/Swiss-Prot: VASP_HUMAN, P50552Tissue specificity: Highly expressed in platelets |
Orthologs for VASP
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for VASP gene from 5/9 species (see all 9
)
About this table Species with no ortholog for VASP
ENSEMBL Gene Tree for VASP | Paralogs for VASP(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for VASP gene
- ENAH2 EVL2
|
SNPs/Variants for VASP(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for VASP (up to first 250kb)
|
Disorders & Mutations for VASP
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 601703
7 Novoseek disease relationships for VASP gene
About this table
|
Medical News for VASP(Possibly Related Articles in
Doctor's Guide)
About This Section
| |
Publications for VASP (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/180 PubMed articles for VASP gene (see all 180
):- Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization. (PubMed id 8812448)1, 2, 3, 4 Zimmer M....Walter U. (1996)
- Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP. (PubMed id 7828592)1, 3, 4 Haffner C....Walter U. (1995)
- The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. (PubMed id 7737110)1, 3, 4 Reinhard M.... Walter U. (1995)
- cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets. (PubMed id 8182057)1, 3, 4 Butt E.... Walter U. (1994)
- Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser 157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets. (PubMed id 16197368)1, 3, 4 Wentworth J.K.... Poole A.W. (2006)
- Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. (PubMed id 15939738)1, 3, 4 Barzik M.... Schafer D.A. (2005)
- Phosphorylation of focal adhesion vasodilator-stimulated phosphoprotein at Ser157 in intact human platelets correlates with fibrinogen receptor inhibition. (PubMed id 7925440)1, 3, 4 Horstrup K....Walter U. (1994)
- The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat. (PubMed id 15569942)1, 3, 4 Kuehnel K.... Strelkov S.V. (2004)
- The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation. (PubMed id 10438535)1, 3, 4 Bachmann C.... Reinhard M. (1999)
- Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity. (PubMed id 10990454)1, 3, 4 Ball L.J.... Jarchau T. (2000)
|
Search for VASP
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing VASP
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing VASP
(According to HUGE)
About This Section
| -- |
Specialized Databases showing VASP(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for VASP(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for VASP:

 | |
 | | | | |
|
 |  |  |
| Antibodies & Assays for VASP  |
| | Recombinant Proteins (VASP) | | Antibodies (VASP) |
| | | Search Tocris compounds for VASP |
|
 |
 | | | |
GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
|