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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

VASH2 Gene

protein-coding   GIFtS: 43
GCID: GC01P213123

vasohibin 2

  Search for VASH2
in our new
 Human Malady Compendium 
Biological research products
for VASH2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Vasohibin 21 2
Vasohibin-Like Protein2 3
FLJ125051
RP11-275G3.12
Vasohibin-21
VASHL3

External Ids:    HGNC: 257231   Entrez Gene: 798052   Ensembl: ENSG000001434947   OMIM: 6104715   UniProtKB: Q86V253   

Export aliases for VASH2 gene to outside databases

Previous GC identifers: GC01P211190 GC01P183798


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: VASH2_HUMAN, Q86V25
Function: Angiogenesis inhibitor. Inhibits network formation by endothelial cells




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_167186.1  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the VASH2 gene promoter:
         AREB6   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidVASH2 promoter sequence
   Search SABiosciences Chromatin IP Primers for VASH2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat VASH2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q32.3   Ensembl cytogenetic band:  1q32.3   HGNC cytogenetic band: 1q23

VASH2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
VASH2 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P213123:  view genomic region     (about GC identifiers)

Start:
213,123,862 bp from pter      End:
213,165,379 bp from pter
Size:
41,518 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: VASH2_HUMAN, Q86V25 (See protein sequence)
Recommended Name: Vasohibin-2  
Size: 355 amino acids; 40450 Da
Developmental stage: Expressed in various embryonic organs at 6 to 12 embryonic weeks. Detected in vessels from 20-week
embryonic organs as well as in endothelial cells from large vessels in neonate
Sequence caution: Sequence=CAH71916.1; Type=Erroneous initiation; Sequence=CAH71918.1; Type=Erroneous initiation;
Secondary accessions: Q2VT46 Q5VTE7 Q5VTE9 Q7Z6E3 Q8IZ24 Q9H9W5
Alternative splicing: 5 isoforms:  Q86V25-1   Q86V25-2   Q86V25-3   Q86V25-4   Q86V25-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for VASH2: NX_Q86V25

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q86V25

  • VASH2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001129946.1  NP_001129947.1  NP_079025.2  

    ENSEMBL proteins: 
     ENSP00000430319   ENSP00000355935   ENSP00000419918   ENSP00000355932   ENSP00000429040  
     ENSP00000428324   ENSP00000355931  

    Human Recombinant Protein Products: 
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    Uscn Proteins for VASH2

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA19204325


    VASH2 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for VASH2


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q86V25

    UniProtKB/Swiss-Prot: VASH2_HUMAN, Q86V25
    Similarity: Belongs to the vasohibin family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: VASH2_HUMAN, Q86V25
    Function: Angiogenesis inhibitor. Inhibits network formation by endothelial cells
    Induction: By VEGF

    miRNA
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    hsa-miR-3678-3p hsa-miR-520f hsa-miR-300 hsa-miR-106a hsa-miR-128 hsa-miR-200a hsa-miR-138-2* hsa-miR-759
    SwitchGear 3'UTR luciferase reporter plasmidVASH2 3' UTR sequence
    Inhib. RNA
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    2 GenomeRNAi human phenotypes for VASH2:
     Decreased viability with pacli  Elongated cells 

    Animal Models:
         Mouse knock-out Vash2tm1Ysat for VASH2
         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Vash2):
     cardiovascular system 

    VASH2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for VASH2

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001938positive regulation of endothelial cell proliferation IDA19204325
    GO:0045766positive regulation of angiogenesis IDA19204325


    VASH2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for VASH2
    Search CenterWatch for drugs/clinical trials and news about VASH2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for VASH2 gene (3 alternative transcripts): 
    NM_001136474.1  NM_001136475.1  NM_024749.3  

    Unigene Cluster for VASH2:

    Vasohibin 2
    Hs.96885  [show with all ESTs]
    Unigene Representative Sequence: NM_024749
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000366966 ENST00000366968(uc001hjx.3 uc010ptn.2 uc001hjw.3 uc001hjy.3)
    ENST00000490792 ENST00000366965 ENST00000271776(uc001hjv.2) ENST00000366967
    ENST00000493155 ENST00000517399 ENST00000481097 ENST00000366969 ENST00000519906
    ENST00000366964(uc001hju.2)

    miRNA
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    8/96 QIAGEN miScript miRNA Assays for microRNAs that regulate VASH2 (see all 96):
    hsa-miR-3678-3p hsa-miR-520f hsa-miR-300 hsa-miR-106a hsa-miR-128 hsa-miR-200a hsa-miR-138-2* hsa-miR-759
    SwitchGear 3'UTR luciferase reporter plasmidVASH2 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for VASH2 (see all 7)
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    OriGene siRNA: VASH2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat VASH2
    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for VASH2 (see all 5)
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat VASH2
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    Additional cDNA sequence: 

    AK022567.1 AK096605.1 AK290973.1 AK302675.1 AY834202.1 BC051856.1 

    8 DOTS entries:

    DT.100018664  DT.91661796  DT.121412716  DT.100710908  DT.100754549  DT.121412694  DT.95260350  DT.91657694 

    24/55 AceView cDNA sequences (see all 55):

    AI126990 BE350485 AW959420 AU121363 AA626409 BC028194 AI198593 NM_024749 
    AK022567 AU124123 BC051856 AI291607 AI961235 AK096605 BX098851 BP368754 
    AA885478 BQ223577 CB990557 AI192696 AI400615 AA025824 AW169104 BC053836 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for VASH2 (see all 6)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11
    SP1:        -           -     -     -           -           -                             -           -         
    SP2:        -     -     -     -     -     -     -                 -                                             
    SP3:                                            -           -                                                   
    SP4:                    -     -     -                                                                           
    SP5:        -                                                                                                   


    ECgene alternative splicing isoforms for VASH2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    VASH2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGACTTTAAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    VASH2 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 3 LifeMap Cells 
    NameCategory
    PureStem™ progenitor EN7 (Embryonic Progenitor Cell)
    Line H9 (WA09) (Embryonic Stem Cell)Early Embryo, Inner Cell Mass
    Matrigel embedded cells (Derivation of cardio...)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See VASH2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for VASH2

    SOURCE GeneReport for Unigene cluster: Hs.96885
        SABiosciences Custom PCR Arrays for VASH2
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for VASH2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for VASH2 gene from 3/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves VASH21 vasohibin 2 72.6(n)
    77.33(a)
      421364  XM_419424.3  XP_419424.3 
    lizard
    (Anolis carolinensis)
    Reptilia VASH26
    --
    71(a)
    1 ↔ 1
    1(238768303-238803048)
    zebrafish
    (Danio rerio)
    Actinopterygii vash21 vasohibin 2 64.58(n)
    64.11(a)
      562668  NM_001025523.1  NP_001020694.1 


    ENSEMBL Gene Tree for VASH2 (if available)
    TreeFam Gene Tree for VASH2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for VASH2 gene
    VASH12  
    1 SIMAP similar gene for VASH2 using alignment to 3 protein entries:     VASH2_HUMAN (see all proteins):
    VASH1

    VASH2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/770 NCBI SNPs in VASH2 are shown (see all 770    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs574789511,2
    F,--213121929(+) TTCTCG/ACAAGA 3 -- us2k11Minor allele frequency- A:0.06WA 118
    rs1815879801,2
    --213121978(+) CTGATA/GATTAG 3 -- us2k10--------
    rs1467060321,2
    --213121982(+) TAATTA/GGTGAT 3 -- us2k10--------
    rs1168278461,2
    --213122121(+) TGGATA/GCTGGT 3 -- us2k11Minor allele frequency- G:0.01WA 118
    rs1152088991,2
    F,--213122138(+) TCAGAT/CGCACA 3 -- us2k12Minor allele frequency- C:0.02NA EA 240
    rs1873872621,2
    --213122189(+) ACTCCA/GCTGAT 3 -- us2k10--------
    rs1390021041,2
    --213122211(+) CTGTGA/CAGACC 3 -- us2k10--------
    rs1498845811,2
    --213122234(+) ATTAGG/TTCTCA 3 -- us2k10--------
    rs594544011,2
    --213122373(+) TTAAGT/CCTTTG 3 -- us2k12Minor allele frequency- C:0.04CSA WA 119
    rs743448651,2
    C,--213122527(+) ACAAGG/TTCTCG 3 -- us2k11Minor allele frequency- T:0.50WA 2

    HapMap Linkage Disequilibrium report for VASH2 (213123862 - 213165379 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for VASH2
         1 CNV: 71207

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    VASH2 for disorders           About MalaCards

    VASH2 for disorders           About GeneDecksing

    OMIM gene information: 610471    OMIM disorders: --


    Export disorders for VASH2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for VASH2 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with VASH2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation and characterization of vasohibin-2 as a homologue of VEGF- inducible endothelium-derived angiogenesis inhibitor vasohibin. (PubMed id 16528006)1, 2, 3 Shibuya T....Sato Y. (2006)
    2. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
    6. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    7. Distinctive localization and opposed roles of vasohib in-1 and vasohibin-2 in the regulation of angiogenesis. (PubMed id 19204325)1 Kimura H....Sato Y. (2009)
    8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 79805 HGNC: 25723 AceView: FLJ12505 Ensembl:ENSG00000143494 euGenes: HUgn79805
    ECgene: VASH2 H-InvDB: VASH2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for VASH2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for VASH2 gene:
    Search GeneIP for patents involving VASH2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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