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VASH1 Gene

protein-coding   GIFtS: 50

GC14P076299
vasohibin 1
(Previous name: KIAA1036 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: KIAA1036)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
KIAA1036 1, 2, 3
VASH 3
Descriptions
vasohibin 1 2
External Ids
HGNC: 199641
Entrez Gene: 228462
UniProtKB: Q7L8A93
Ensembl: ENSG000000712467
Search outside databases for aliases for VASH1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: VASH1_HUMAN, Q7L8A9
Function: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by
endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial
cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect
the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts
in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits
heparin-binding activity

Gene Wiki entry for VASH1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VASH1 gene  

Entrez Gene cytogenetic band: 14q24.3   Ensembl cytogenetic band:  14q24.3   HGNC cytogenetic band: 14q24.3

VASH1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P076299:     (about GC identifiers)

Start:
76,298,285 bp from pter
End:
76,319,111 bp from pter
Size:
20,827 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: VASH1_HUMAN, Q7L8A9 (See protein sequence)
Recommended Name: Vasohibin-1  
Size: 365 amino acids; 40957 Da
Subcellular location: Secreted
Caution: Although probably secreted, it lacks a canonical signal sequence
Sequence caution: Sequence=AAD44361.1; Type=Erroneous gene model prediction;
Secondary accessions: Q96H02 Q9UBF4 Q9Y629
Alternative splicing: 2 isoforms:  Q7L8A9-1   Q7L8A9-2   

Post-translational modifications:

  • 2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic
    cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems
    to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318
    may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the
    36 kDa form1


  • REFSEQ proteins: NP_055724.1  

    ENSEMBL proteins: 
    ENSP00000167106 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (VASH1)
    Human Recombinant Proteins from Abnova (VASH1)
                  OriGene Purified Recombinant Human Protein: VASH1 

    5 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005615 extracellular space IDA15467828
    GO:0005634 nucleus IDA18029348
    GO:0005737 cytoplasm IEA--
    GO:0005783 endoplasmic reticulum IDA15467828
    About this table

    Antibodies for VASH1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for VASH1
    R&D Systems Antibodies for VASH1 (Vasohibin)
    Antibodies from Abcam (VASH1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (VASH1)
    Novus Biologicals Antibodies for VASH1

    Assays for VASH1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q7L8A9

    UniProtKB/Swiss-Prot: VASH1_HUMAN, Q7L8A9
    Similarity: Belongs to the vasohibin family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VASH1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VASH1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014909

                  Applied Biosystems Silencer® siRNAs for VASH1

                  Sigma-Aldrich siRNA for VASH1  
                         Sigma-Aldrich shRNA for VASH1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for VASH1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014909
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014909
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_014909 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014909

    UniProtKB/Swiss-Prot: VASH1_HUMAN, Q7L8A9
    Function: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by
    endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial
    cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect
    the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts
    in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits
    heparin-binding activity
    Induction: By VEGF

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Vash1):


    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001937 negative regulation of endothelial cell proliferation IDA15467828
    GO:0007049 cell cycle IEA--
    GO:0007050 cell cycle arrest IEA--
    GO:0016525 negative regulation of angiogenesis IDA15467828
    GO:0043537 negative regulation of blood vessel endothelial cell migration IDA15467828
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for VASH1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VASH1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VASH1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014909

                  Sigma-Aldrich siRNA for VASH1  
                         Sigma-Aldrich shRNA for VASH1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014909  

    REFSEQ mRNAs for VASH1 gene: 

    NM_014909.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014909  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014909
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014909
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_014909 

    Additional cDNA sequence: 

    AB028959.1 AF055021.1 AK094330.1 AK291377.1 AL832588.2 BC009031.1 BC051896.1 

    9 DOTS entries:

    DT.453848  DT.311953  DT.70100318  DT.108020  DT.65284442  DT.100654054  DT.95241664  DT.100781249 
    DT.95375355 

    24/166 AceView cDNA sequences (see all 166 ):

    AI338488 AI990120 BE349025 AF055021 AI694412 BE222175 BU782643 BE674799 
    BU621127 AA115240 BM675057 BQ233634 AI801580 CA308259 AI873697 BE220471 
    AW236989 CA448293 BM713191 AK094330 CD626309 BM708077 AW206719 BC051896 

    highest scoring ESTs for VASH1:

    AB028959 AA043953 AA043965 AA142893 AA622153 AA704552 AA808078 AA813421 AA887003 AI360738 

    Unigene Cluster for VASH1:

    Vasohibin 1
    Hs.525479  [show with all ESTs]
    Unigene Representative Sequence: NM_014909


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000167106  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    VASH1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for VASH1

    1 / 2 / 3

    10 probe-sets matching VASH1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    54473_at2, 3 U95-B 1 1.00 1.00 0.84 0.56 AA142893 0.80 1.00 0.91 1
    40266_at2, 3 U95-A 2 1.00 0.97 0.84 1.05 AB028959 1.00 1.00 1.00 1

    90555_at2, 3 U95-D 2 0.50 0.88 0.55 1.41 AI807656 0.60 1.00 0.82 1

    203940_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014909 0.60 1.00 0.82 1

    230546_at2, 3 U133-B 1 1.00 1.00 -- -- AA142893 0.80 1.00 0.91 1

    242566_at*2 U133-B NULL 0.00 0.00 -- -- -- -- -- -- --

    203940_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    230546_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1556423_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    242566_at*2 U133Plus2 NULL 0.00 0.00 -- -- -- -- -- -- --
    GeneDecks  VASH1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGTGTGCTTG

    SOURCE GeneReport for Unigene cluster: Hs.525479

    Expression variation in blood from EXPOLDB for VASH1

    UniProtKB/Swiss-Prot: VASH1_HUMAN, Q7L8A9
    Tissue specificity: Preferentially expressed in endothelial cells. Highly expressed in fetal
    organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected
    in microvessels endothelial cells of atherosclerotic lesions

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for VASH1 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    VASH11   -- vasohibin 1 93.85(n)
    95.59(a)
    490799  XM_849075.1  XP_854168.1 
    chimpanzee
    (Pan troglodytes)
    VASH11   -- vasohibin 1 99.18(n)
    99.18(a)
    467514  XM_522911.2  XP_522911.2 
    rat
    (Rattus norvegicus)
    Vash11   -- vasohibin 1 88.31(n)
    92.6(a)
    503052  XM_578575.2  XP_578575.2 
    mouse
    (Mus musculus)
    Vash11, 5 125
    vasohibin 11, 5 90.77(n)1
    96.19(a)1
    2383281  NM_177354.31  NP_796328.11 
     AB2849485  AI8349785  (see all 11)
    zebrafish
    (Danio rerio)
    LOC1000017481   -- hypothetical LOC100001748 70.4(n)
    68.97(a)
    100001748  XM_001921417.1  XP_001921452.1 
    About this table        Species with no ortholog for VASH1

    ENSEMBL Gene Tree for VASH1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for VASH1 gene
    VASH22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/120 NCBI SNPs in VASH1 are shown (see all 120 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 66)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 14 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs7305481,2
    A,C,F,H,O76296474(+) GCTACG/CGGAGC 1 -- ng5118Minor allele frequency- C:0.31NA EA MN EU WA 1688
    rs22807961,2
    A,C,F,H76316511(+) gtaaaT/Cggatg 1 -- ut31 ese35Minor allele frequency- C:0.35EA EU WA 1916
    rs116264231,2
    A,C,F,H76319420(+) CTTCCG/AGTCTC 1 -- ng3118Minor allele frequency- A:0.30EA NA EU WA 1652
    rs22426221,2
    A,C,F,H76319247(+) CCAGCC/GCCTGC 1 -- ng311Minor allele frequency- G:0.19WA 120
    rs171048691,2
    C,F,H76318232(+) CTGGGG/AAGCCA 1 -- ut3111Minor allele frequency- A:0.04NA EU EA WA 978
    rs118475661,2
    C,F,H76296407(+) TGGAGT/ACTTGC 1 -- ng517Minor allele frequency- A:0.07EU EA WA NA 540
    rs9732271,2
    A,C,F76296887(+) CCAGCG/CGACCC 1 -- ng515Minor allele frequency- C:0.48EA NA 232
    rs171048661,2
    F,H76315397(+) TGATGG/TTGGGA 1 -- ut31 ese38Minor allele frequency- T:0.15NA EU EA WA 636
    rs22807951,2
    C,F76315773(+) GAGGAG/AGGGTG 1 -- ut31 ese3 trp34Minor allele frequency- A:0.11EA NA 1446
    rs128866001,2
    H76315936(+) GGCCAG/CCATGG 1 -- ut31 ese34Minor allele frequency- C:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for VASH1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 609011

    1 Novoseek disease relationship for VASH1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0.00 2 15467828 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/24 PubMed articles for VASH1 gene (see all 24 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 22846 HGNC: 19964 AceView: KIAA1036 Ensembl:ENSG00000071246 euGenes: HUgn22846
    ECgene: VASH1 H-InvDB: VASH1
    (According to HUGE)
    About This Section

    HUGE: KIAA1036
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for VASH1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Predesigned and custom siRNAs for VASH1 Antibodies for VASH1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for VASH1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (VASH1)
     Antibodies (VASH1)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for VASH1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for VASH1

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