V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

VAMP5 Gene

protein-coding   GIFtS: 60

GC02P085665
vesicle-associated membrane protein 5 (myobrevin)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
Myobrevin 3
VAMP-5 3
myobrevin 2
Descriptions
vesicle-associated membrane protein 5 2
vesicle-associated membrane protein 5 (myobrevin) 2
External Ids
HGNC: 126461
Entrez Gene: 107912
UniProtKB: O951833
Ensembl: ENSG000001688997
Search outside databases for aliases for VAMP5 gene

Previous GC identifers: GC02P085706 GC02P086105 GC02P085786 GC02P085723

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for VAMP5:
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main
components of a protein complex involved in the docking and/or fusion of vesicles and cell
membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein
(VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in
vesicle trafficking events that are associated with myogenesis. [provided by RefSeq]

UniProtKB/Swiss-Prot: VAMP5_HUMAN, O95183
Function: May participate in trafficking events that are associated with myogenesis, such as
myoblast fusion and/or GLUT4 trafficking

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the VAMP5 gene  

Entrez Gene cytogenetic band: 2p11.2   Ensembl cytogenetic band:  2p11.2   HGNC cytogenetic band: 2p11.2

VAMP5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P085665:     (about GC identifiers)

Start:
85,665,042 bp from pter
End:
85,674,022 bp from pter
Size:
8,981 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: VAMP5_HUMAN, O95183 (See protein sequence)
Recommended Name: Vesicle-associated membrane protein 5  
Size: 116 amino acids; 12805 Da
Subcellular location: Cell membrane; Single-pass type IV membrane protein (Probable). Endomembrane
system; Single-pass type IV membrane protein (Probable). Golgi apparatus, trans-Golgi network
membrane; Single-pass type IV membrane protein (Probable). Note=Associated with the plasma
membrane as well as intracellular perinuclear and peripheral vesicular structures of myotubes.
Associated with the trans-Golgi, but not with the cis-Golgi apparatus (By similarity)
Secondary accessions: Q9P0T2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006625.1  

    ENSEMBL proteins: 
    ENSP00000305647 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (VAMP5)
    Human Recombinant Proteins from Abnova (VAMP5)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005794 Golgi apparatus IEA--
    GO:0005886 plasma membrane TAS9725904
    GO:0005887 integral to plasma membrane IEA--
    GO:0012505 endomembrane system IEA--
    About this table

    Antibodies for VAMP5: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for VAMP5
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (VAMP5), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibodies for VAMP5

    Assays for VAMP5: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR016444 Synaptobrevin_met/fun
     IPR001388 Synaptobrevin


       GeneDecks  VAMP5 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O95183

    ProtoNet protein and cluster: O95183

    1 Blocks protein family: IPB001388 Synaptobrevin

    UniProtKB/Swiss-Prot: VAMP5_HUMAN, O95183
    Similarity: Belongs to the synaptobrevin family
    Similarity: Contains 1 v-SNARE coiled-coil homology domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VAMP5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VAMP5)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006634

                  Applied Biosystems Silencer® siRNAs for VAMP5

                  Sigma-Aldrich siRNA for VAMP5  
                         Sigma-Aldrich shRNA for VAMP5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for VAMP5
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006634
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006634
                                     untagged cDNA clone in CMV expression vector: NM_006634 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006634

    UniProtKB/Swiss-Prot: VAMP5_HUMAN, O95183
    Function: May participate in trafficking events that are associated with myogenesis, such as
    myoblast fusion and/or GLUT4 trafficking
    Induction: During myogenesis

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for VAMP5):
     hsa04130 SNARE interactions in vesicular transport

       GeneDecks  VAMP5 for the pathways selected above  
    About GeneDecksing

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007275 multicellular organismal development IEA--
    GO:0007517 muscle development TAS9725904
    GO:0007519 skeletal muscle development IEA--
    GO:0016192 vesicle-mediated transport IEA--
    GO:0030154 cell differentiation IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for VAMP5

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (VAMP5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (VAMP5)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006634

                  Sigma-Aldrich siRNA for VAMP5  
                         Sigma-Aldrich shRNA for VAMP5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006634  

    REFSEQ mRNAs for VAMP5 gene: 

    NM_006634.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006634  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006634
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006634
                                     untagged cDNA clone in CMV expression vector: NM_006634 

    Additional cDNA sequence: 

    AF054825.1 AF077197.1 AF151025.1 AK292660.1 BC017891.1 CR542215.1 

    3 DOTS entries:

    DT.314218  DT.100748293  DT.92431991 

    24/316 AceView cDNA sequences (see all 316 ):

    AA305331 W80846 BX092149 AA634593 BQ675243 BM707971 AA309787 AA371270 
    AA365845 AI079353 BM761526 CB156929 BM702226 AI418592 AI568721 F27921 
    BI836255 BM887680 BC017891 N90862 CB108371 CD369345 NM_006634 BQ021316 

    highest scoring ESTs for VAMP5:

    AF054825 AA056741 AA147362 AA301711 AA309787 AA341743 AA365845 AA521036 AA595920 AA641625 

    Unigene Cluster for VAMP5:

    Vesicle-associated membrane protein 5 (myobrevin)
    Hs.534373  [show with all ESTs]
    Unigene Representative Sequence: BG535701


    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for VAMP5

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b · 3c
    SP1:              -                           
    SP2:              -                           
    SP3:                                          

    About this scheme

    ECgene alternative splicing isoforms for VAMP5

    1 Ensembl transcript including schematic representation:
    ENST00000306384  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    VAMP5 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for VAMP5

    1 / 2 / 3

    3 probe-sets matching VAMP5 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    32533_s_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF054825 1.00 0.90 0.95 1

    204929_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006634 0.60 0.83 0.73 1

    204929_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  VAMP5 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTGGGCCAGC

    SOURCE GeneReport for Unigene cluster: Hs.534373

    Expression variation in blood from EXPOLDB for VAMP5

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for VAMP5 gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    VAMP51   -- vesicle-associated membrane protein 5 (myobrevin) 83.77(n)
    76.52(a)
    482823  XM_539938.2  XP_539938.2 
    chimpanzee
    (Pan troglodytes)
    VAMP51   -- vesicle-associated membrane protein 5 (myobrevin) 99.14(n)
    98.28(a)
    470422  XM_525803.2  XP_525803.2 
    cow
    (Bos taurus)
    VAMP51   -- vesicle-associated membrane protein 5 (myobrevin) 82.9(n)
    75.65(a)
    540406  NM_001046476.1  NP_001039941.1 
    rat
    (Rattus norvegicus)
    Vamp51   -- vesicle-associated membrane protein 5 78(n)
    72(a)
    89818  NM_053555.1  NP_446007.1 
    mouse
    (Mus musculus)
    Vamp51, 5 65
    vesicle-associated membrane protein 51, 5 79.33(n)1
    74(a)1
    536201  NM_001080742.11  NP_001074211.11 
     AF0356435  AF1193845  (see all 11)
    About this table        Species with no ortholog for VAMP5

    ENSEMBL Gene Tree for VAMP5
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for VAMP5 gene
    VAMP82  VAMP42  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/51 NCBI SNPs in VAMP5 are shown (see all 51 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 63)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs15611981,2
    A,C,F,H,O85663500(-) AATCTG/AGTCCC 1 -- ng515Minor allele frequency- A:0.47MN EU EA WA 600
    rs12549011,2
    A,C,F,H85664740(-) tcaggC/Taccat 1 -- ng519Minor allele frequency- T:0.20MN EU EA WA 1020
    --
    rs75939691,2
    A,C,F,H85663181(+) AAGGCT/CTCTGG 1 -- ng514Minor allele frequency- C:0.37EU EA WA 420
    --
    rs142421,2
    A,C,F,H85673923(-) GAAATG/ACAGGG 1 -- ut31 ese39Minor allele frequency- A:0.23EA MN EU WA NA 1492
    rs15623231,2
    A,C,F85663222(-) ATGCCA/GAGGAG 1 -- ng511Minor allele frequency- G:0.28MN 184
    rs76080741,2
    A,C85663214(+) AAGCAG/TCTCTC 1 -- ng510--------
    --
    rs23666401,2
    A,C85664245(+) ACAAAA/CAAAAA 1 -- ng51 tfbs3 trp30--------
    rs23666391,2
    A,C85663466(+) CAGGAA/GGAAGG 1 -- ng51 trp30--------
    --
    rs10493591,2
    F85673884(-) CTCACT/CGGGGT 1 -- ut31 ese31Minor allele frequency- C:0.02MN 184
    --
    rs101873731,2
    A85664248(+) AAAAAA/CAAAAA 1 -- ng51 trp30--------
    About this table

    HapMap Linkage Disequilibrium images for VAMP5 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607029

    1 PharmGKB disease relationship for VAMP5 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    Genetic Association Database: VAMP5
    Human Genome Epidemiology Navigator: VAMP5 (6 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for VAMP5 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10791 HGNC: 12646 AceView: VAMP8andVAMP5 Ensembl:ENSG00000168899 euGenes: HUgn10791
    ECgene: VAMP5 H-InvDB: VAMP5
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for VAMP5:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for VAMP5-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for VAMP5 Antibodies for VAMP5
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for VAMP5 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (VAMP5)
     Search for Antibodies
     Chimera RNAi (VAMP5)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (VAMP5)
     Antibodies (VAMP5)
     Search OriGene for VAMP5
     Search Tocris compounds for VAMP5




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for VAMP5

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    VAMP5 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com