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Aliases & Descriptions for VAC14
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases ArPIKfyve 1 , 2 FLJ10305 1 , 2 FLJ36622 2 FLJ46582 2 MGC149815 2 MGC149816 2 TAX1BP2 2 , 3 , 5 TRX 2 , 3 , 5
Descriptions Tax1 (human T-cell leukemia virus type I) binding protein 1 2 Tax1 (human T-cell leukemia virus type I) binding protein 2 1 , 2 Tax1-binding protein 2 3 Vac14 homolog 2 Vac14 homolog (S. cerevisiae) 2
Search outside databases for aliases for VAC14 gene
Summaries for VAC14 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for VAC14 : Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. Aregulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 byactivating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in thebreakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM]
Genomic Location for VAC14
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the VAC14 gene Entrez Gene cytogenetic band: 16q22.1 Ensembl cytogenetic band: 16q22.1 HGNC cytogenetic band: 16q22.1 VAC14 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 16 GeneLoc Exon Structure
GeneLoc location for GC16M069280:
(about GC identifiers )
Start:
69,278,843 bp from pter
End:
69,392,572 bp from pter
Size:
113,730 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000016.8 NT_010498.15 Proteins for VAC14
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: VAC14_HUMAN, Q08AM6 (See
protein sequence )Recommended Name: Protein VAC14 homolog Size : 782 amino acids; 87973 Da
Sequence caution : Sequence=AAB03813.1; Type=Miscellaneous discrepancy; Note=Unknown C-terminalregion;
Secondary accessions : B3KPJ5 Q13174 Q6IA12 Q7L4Y1 Q9BW96 Q9H6V6
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins: NP_060522.3 ENSEMBL proteins: ENSP00000254957 ENSP00000261776 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 1 Gene Ontology (GO) cellular component term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005575 cellular_component
ND --
About this table Antibodies for VAC14: Assays for VAC14:
Protein
Domains/ Families for VAC14(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q08AM6 ProtoNet protein and cluster: Q08AM6
1 Blocks protein family : IPB000357 HEAT repeat UniProtKB/Swiss-Prot: VAC14_HUMAN, Q08AM6 Similarity : Belongs to the VAC14 familySimilarity : Contains 6 HEAT repeats
Gene Function for VAC14
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
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Sigma-Aldrich ,
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Clones , and
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Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018052 Applied Biosystems Silencer ® siRNAs for VAC14 Sigma-Aldrich siRNA for VAC14 Sigma-Aldrich shRNA for VAC14 Explore Sigma-Aldrich super-pooled esiRNAs               OriGene GFP tagged cDNA clone in CMV expression vector: NM_018052                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_018052                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_018052  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_018052 UniProtKB/Swiss-Prot: VAC14_HUMAN, Q08AM6 Induction : Interacts with HTLV-1 Tax
7 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Vac14) :2 Gene Ontology (GO) molecular function terms (links to tree view) :
About this table
Pathways & Interactions for VAC14
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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Gene Network CentralTM Interacting Genes and Proteins Network for VAC14 5/6 Interacting proteins for VAC14 (ENSP00000261776 3 Q08AM6 1 , 2 ) via UniProtKB, MINT, and/or STRING (see all 6
)About this table 2 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0007165 signal transduction
NAS 8611628 GO:0044419 interspecies interaction between organisms
IEA --
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Drugs & Compounds for VAC14 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for VAC14
Transcripts for VAC14(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
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siRNAs from Applied Biosystems ,
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shRNA from
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OriGene ,
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018052 Sigma-Aldrich siRNA for VAC14 Sigma-Aldrich shRNA for VAC14 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_018052
REFSEQ mRNAs for VAC14 gene: NM_018052.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_018052
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_018052                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_018052                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_018052  
Additional cDNA sequence: AK001167.1 AK025479.1 AK056433.1 AK090627.1 AK128439.1 AK297564.1 AK307766.1 AK310466.1 AL137396.1 BC000536.2 BC007214.1 BC110072.1 BC110852.1 BC125108.1 BC125109.1 CR457343.1 U25801.1
17 DOTS entries : DT.444549 DT.100025998 DT.99987608 DT.100794472 DT.402903 DT.120699564 DT.97809091 DT.102830706 DT.120699558 DT.75112160 DT.91674240 DT.100832028 DT.120699514 DT.87046896 DT.95326400 DT.97847456 DT.99963761
24/258 AceView cDNA sequences (see all 258
):BU557063 CF272267 CB105697 CA307115 BX344569 BQ722099 AI150352 BM904117 BU732158 AI984151 BU509412 NM_018052 AW138702 BU737693 AK056433 BM472596 CA307130 CA425376 AW964160 CA389947 BM716155 BM712224 BU845675 H93425
highest scoring ESTs for VAC14 :AI075995 BE728471 BE893810 BG107035 BG473591 BG479581 BM146195 BM147550 BM148120 BM148245
Unigene Cluster for VAC14: Vac14 homolog (S. cerevisiae) Hs.445061 [show with all ESTs ] Unigene Representative Sequence: AK128439 GeneLoc Exon Structure 5/8 Alternative Splicing Database (ASD) splice patterns (SP) for VAC14 (see all 8
) ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b ^ 12a · 12b ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ SP1 :                                                     SP2 :                                                     SP3 :                                           -   -         SP4 :                                           -   -     -     SP5 :                                                    
ExUns: 18 ^ 19 ^ 20a · 20b SP1 :         SP2 :         SP3 :         SP4 :         SP5 :        
About this scheme ECgene alternative splicing isoforms for VAC14 2 Ensembl transcripts including schematic representations : ENST00000254957
ENST00000261776
Expression for VAC14
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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VAC14 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for VAC14 1 / 2 / 3
7 probe-sets matching VAC14 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: CCTGTGTGTGSOURCE GeneReport for Unigene cluster: Hs.445061 UniProtKB/Swiss-Prot: VAC14_HUMAN, Q08AM6 Tissue specificity : Ubiquitously expressed
Orthologs for VAC14
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for VAC14 gene from 5/19 species (see all 19
)
About this table Species with no ortholog for VAC14 ENSEMBL Gene Tree for VAC14 Paralogs for VAC14 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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SNPs/Variants for VAC14 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for VAC14 (up to first 250kb)
Disorders & Mutations for VAC14
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604632
Medical News for VAC14 (Possibly Related Articles in
Doctor's Guide )
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Publications for VAC14 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/15 PubMed articles for VAC14 gene (see all 15
): A mammalian ortholog of Saccharomyces cerevisiae Vac14 that associates with and up-regulates PIKfyve phosphoinositide 5-kinase activity. (PubMed id 15542851) 1, 2, 3 Sbrissa D....Shisheva A. (2004) Isolation of a cDNA clone, TRX encoding a human T-cell lymphotrophic virus type-I Tax1 binding protein. (PubMed id 8611628) 1, 3, 4 Mireskandari A....Brady J.N. (1996) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3, 4 Ota T....Sugano S. (2004) Congenital hydrocephalus in hy3 mice is caused by a frameshift mutation in Hydin, a large novel gene. (PubMed id 12719380) 2, 3 Davy B.E. and Robinson M.L. (2003) Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex. (PubMed id 17556371) 1, 3 Sbrissa D....Shisheva A. (2007) Binding of Vac14 to neuronal nitric oxide synthase: Characterisation of a new internal PDZ-recognition motif. (PubMed id 17161399) 1, 3 Lemaire J.F. and McPherson P.S. (2006) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. (PubMed id 18691976) 4 Daub H.... Mann M. (2008) ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality. (PubMed id 18950639) 3 Sbrissa D....Shisheva A. (2008) Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice. (PubMed id 17956977) 3 Zhang Y....Weisman L.S. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. (PubMed id 17353931) 3 Ewing R.M....Figeys D. (2007)
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Genome Databases showing VAC14
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing VAC14
(According to HUGE )
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Specialized Databases showing VAC14 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for VAC14 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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