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Aliases for USP5 Gene

Aliases for USP5 Gene

  • Ubiquitin Specific Peptidase 5 (Isopeptidase T) 2 3
  • ISOT 3 4 6
  • Ubiquitin Specific Protease 5 (Isopeptidase T) 2 3
  • Ubiquitin-Specific-Processing Protease 5 3 4
  • Deubiquitinating Enzyme 5 3 4
  • Ubiquitin Thioesterase 5 3 4
  • Isopeptidase T 3 4
  • Ubiquitin-Specific Protease-5 (Ubiquitin Isopeptidase T) 3
  • Ubiquitin Carboxyl-Terminal Hydrolase 5 3
  • Ubiquitin Thiolesterase 5 3
  • Ubiquitin Isopeptidase T 3
  • EC 3.4.19.12 4
  • EC 3.1.2.15 64

External Ids for USP5 Gene

Summaries for USP5 Gene

Entrez Gene Summary for USP5 Gene

  • Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

GeneCards Summary for USP5 Gene

USP5 (Ubiquitin Specific Peptidase 5 (Isopeptidase T)) is a Protein Coding gene. Among its related pathways are Ubiquitin-Proteasome Dependent Proteolysis. GO annotations related to this gene include ubiquitin thiolesterase activity and omega peptidase activity. An important paralog of this gene is USP13.

UniProtKB/Swiss-Prot for USP5 Gene

  • Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored Lys-48-linked polyubiquitin disassembly. Binds linear and Lys-63-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Gene Wiki entry for USP5 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for USP5 Gene

Genomics for USP5 Gene

Genomic Location for USP5 Gene

Start:
6,852,121 bp from pter
End:
6,866,632 bp from pter
Size:
14,512 bases
Orientation:
Plus strand

Genomic View for USP5 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for USP5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP5 Gene

Regulatory Elements for USP5 Gene

Proteins for USP5 Gene

  • Protein details for USP5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P45974-UBP5_HUMAN
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 5
    Protein Accession:
    P45974
    Secondary Accessions:
    • D3DUS7
    • D3DUS8
    • Q96J22

    Protein attributes for USP5 Gene

    Size:
    858 amino acids
    Molecular mass:
    95786 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion.;
    Quaternary structure:
    • Interacts with TRIML1.
    Miscellaneous:
    • The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer

    Three dimensional structures from OCA and Proteopedia for USP5 Gene

    Alternative splice isoforms for USP5 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for USP5 Gene

Proteomics data for USP5 Gene at MOPED

Post-translational modifications for USP5 Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys20, Lys163, Lys184, Lys318, Lys353, Lys357, Lys360, Lys391, Lys423, Lys553, Lys575, Lys586, Lys743, Lys793, and Lys836

Other Protein References for USP5 Gene

Domains for USP5 Gene

Gene Families for USP5 Gene

HGNC:
  • USP :Ubiquitin-specific peptidases

UniProtKB/Swiss-Prot:

UBP5_HUMAN
Domain:
  • The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition:
    • P45974
  • Contains 2 UBA domains.:
    • P45974
  • Contains 1 USP domain.:
    • P45974
Family:
  • Belongs to the peptidase C19 family.:
    • P45974
Similarity:
  • Contains 1 UBP-type zinc finger.:
    • P45974
genes like me logo Genes that share domains with USP5: view

Function for USP5 Gene

Molecular function for USP5 Gene

GENATLAS Biochemistry: ubiquitin specific protease 5,100kDa,expressed in brain,deubiquitin enzyme family
UniProtKB/Swiss-Prot CatalyticActivity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)
UniProtKB/Swiss-Prot Function: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored Lys-48-linked polyubiquitin disassembly. Binds linear and Lys-63-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Enzyme Numbers (IUBMB) for USP5 Gene

Gene Ontology (GO) - Molecular Function for USP5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity TAS 7578059
GO:0004221 ubiquitin thiolesterase activity --
GO:0004843 ubiquitin-specific protease activity IBA --
GO:0005515 protein binding IPI 16189514
GO:0008242 omega peptidase activity IEA --
genes like me logo Genes that share ontologies with USP5: view

Phenotypes for USP5 Gene

MGI mutant phenotypes for USP5:
inferred from 1 alleles
genes like me logo Genes that share phenotypes with USP5: view

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for USP5 Gene

Localization for USP5 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for USP5 Gene COMPARTMENTS Subcellular localization image for USP5 gene
Compartment Confidence
lysosome 4
vacuole 4
cytosol 2
nucleus 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for USP5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005764 lysosome TAS 9827704
genes like me logo Genes that share ontologies with USP5: view

Pathways for USP5 Gene

genes like me logo Genes that share pathways with USP5: view

Pathways by source for USP5 Gene

1 Qiagen pathway for USP5 Gene

Gene Ontology (GO) - Biological Process for USP5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process IEA --
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process IMP 19098288
GO:0071108 protein K48-linked deubiquitination IMP 19098288
genes like me logo Genes that share ontologies with USP5: view

Compounds for USP5 Gene

(1) HMDB Compounds for USP5 Gene

Compound Synonyms Cas Number PubMed IDs
Water
  • Dihydrogen oxide
7732-18-5

(2) Novoseek inferred chemical compound relationships for USP5 Gene

Compound -log(P) Hits PubMed IDs
cysteine 25.4 1
zinc 24.5 4
genes like me logo Genes that share compounds with USP5: view

Transcripts for USP5 Gene

mRNA/cDNA for USP5 Gene

(2) REFSEQ mRNAs :
(6) Additional mRNA sequences :
(9) Selected AceView cDNA sequences:
(7) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for USP5 Gene

Ubiquitin specific peptidase 5 (isopeptidase T):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for USP5 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21a · 21b · 21c
SP1:
SP2: -
SP3:

Relevant External Links for USP5 Gene

GeneLoc Exon Structure for
USP5
ECgene alternative splicing isoforms for
USP5

Expression for USP5 Gene

mRNA expression in normal human tissues for USP5 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for USP5 Gene

SOURCE GeneReport for Unigene cluster for USP5 Gene Hs.631661

genes like me logo Genes that share expressions with USP5: view

Orthologs for USP5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for USP5 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia USP5 36
  • 98.75 (n)
  • 98.93 (a)
USP5 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia USP5 36
  • 92.42 (n)
  • 98.02 (a)
USP5 37
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia USP5 36
  • 93.67 (n)
  • 98.6 (a)
USP5 37
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Usp5 36
  • 91.96 (n)
  • 98.37 (a)
Usp5 16
Usp5 37
  • 98 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia USP5 37
  • 92 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Usp5 36
  • 91.65 (n)
  • 98.25 (a)
chicken
(Gallus gallus)
Aves USP5 36
  • 81.33 (n)
  • 91.35 (a)
USP5 37
  • 91 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia USP5 37
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4101 36
usp5 36
  • 73.92 (n)
  • 79.69 (a)
zebrafish
(Danio rerio)
Actinopterygii usp5 36
  • 66.87 (n)
  • 70.22 (a)
usp5 37
  • 69 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012248 36
  • 58.94 (n)
  • 54.25 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG12082 36
  • 58.62 (n)
  • 53.61 (a)
CG12082 37
  • 52 (a)
OneToMany
CG12082 38
  • 50 (a)
worm
(Caenorhabditis elegans)
Secernentea usp-5 37
  • 37 (a)
OneToMany
T27A3.2 38
  • 35 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBP14 37
  • 29 (a)
OneToMany
UBP14 39
thale cress
(Arabidopsis thaliana)
eudicotyledons UBP14 36
  • 55.85 (n)
  • 52.04 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.1989 36
corn
(Zea mays)
Liliopsida Zm.5738 36
rice
(Oryza sativa)
Liliopsida Os.7173 36
Os01g0177200 36
  • 55.39 (n)
  • 52.36 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9810 37
  • 49 (a)
OneToMany
Species with no ortholog for USP5:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for USP5 Gene

ENSEMBL:
Gene Tree for USP5 (if available)
TreeFam:
Gene Tree for USP5 (if available)

Paralogs for USP5 Gene

Paralogs for USP5 Gene

Selected SIMAP similar genes for USP5 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for USP5 Gene

genes like me logo Genes that share paralogs with USP5: view

Variants for USP5 Gene

Sequence variations from dbSNP and Humsavar for USP5 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type MAF
rs1126996 -- 6,859,512(+) CCGAC(A/G)GACCC synonymous-codon, reference
rs1802898 -- 6,861,076(+) AGCTG(C/T)CTGTG reference, missense
rs2071059 -- 6,860,567(+) CCTCT(A/G)TCAGC intron-variant
rs2071060 -- 6,864,473(+) TCACG(A/C)GGTCA intron-variant
rs2071061 -- 6,864,487(+) GATCA(A/G)GACCA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for USP5 Gene

Variant ID Type Subtype PubMed ID
nsv832323 CNV Loss 17160897
nsv832324 CNV Loss 17160897
dgv1353n71 CNV Loss 21882294
nsv898641 CNV Loss 21882294
nsv898642 CNV Loss 21882294

Relevant External Links for USP5 Gene

HapMap Linkage Disequilibrium report
USP5

Disorders for USP5 Gene

No disorders were found for USP5 Gene.

No data available for UniProtKB/Swiss-Prot for USP5 Gene

Publications for USP5 Gene

  1. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13. (PMID: 8723724) Ansari-Lari M.A. … Gibbs R.A. (Genome Res. 1996) 2 3 4 23
  2. The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. (PMID: 16564012) Reyes-Turcu F.E. … Wilkinson K.D. (Cell 2006) 3 4 23
  3. Recognition of polyubiquitin isoforms by the multiple ubiquitin binding modules of isopeptidase T. (PMID: 18482987) Reyes-Turcu F.E. … Wilkinson K.D. (J. Biol. Chem. 2008) 3 4 23
  4. Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53. (PMID: 19098288) Dayal S. … Saville M.K. (J. Biol. Chem. 2009) 3 4 23
  5. Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase T. (PMID: 7578059) Wilkinson K.D. … Pickart C.M. (Biochemistry 1995) 3 23

Products for USP5 Gene

  • Addgene plasmids for USP5

Sources for USP5 Gene

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