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Aliases for USP5 Gene

Aliases for USP5 Gene

  • Ubiquitin Specific Peptidase 5 2 3
  • Ubiquitin Specific Peptidase 5 (Isopeptidase T) 2 3 5
  • Isopeptidase T 2 3 4
  • Ubiquitin-Specific-Processing Protease 5 3 4
  • Deubiquitinating Enzyme 5 3 4
  • Ubiquitin Thioesterase 5 3 4
  • ISOT 3 4
  • Ubiquitin-Specific Protease-5 (Ubiquitin Isopeptidase T) 3
  • Ubiquitin Specific Protease 5 (Isopeptidase T) 3
  • Testicular Tissue Protein Li 218 3
  • Ubiquitin Thiolesterase 5 3
  • Ubiquitin Isopeptidase T 3
  • EC 3.4.19.12 4
  • EC 3.1.2.15 63

External Ids for USP5 Gene

Previous GeneCards Identifiers for USP5 Gene

  • GC12M006999
  • GC12M007084
  • GC12P006840
  • GC12P006831
  • GC12P006815
  • GC12P006978
  • GC12P006985
  • GC12P006991
  • GC12P006998
  • GC12P007006
  • GC12P007024
  • GC12P007047
  • GC12P007111
  • GC12P007137

Summaries for USP5 Gene

Entrez Gene Summary for USP5 Gene

  • Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

GeneCards Summary for USP5 Gene

USP5 (Ubiquitin Specific Peptidase 5) is a Protein Coding gene. Among its related pathways are Ubiquitin-Proteasome Dependent Proteolysis. GO annotations related to this gene include cysteine-type endopeptidase activity and thiol-dependent ubiquitinyl hydrolase activity. An important paralog of this gene is USP13.

UniProtKB/Swiss-Prot for USP5 Gene

  • Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored Lys-48-linked polyubiquitin disassembly. Binds linear and Lys-63-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Gene Wiki entry for USP5 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for USP5 Gene

Genomics for USP5 Gene

Regulatory Elements for USP5 Gene

Enhancers for USP5 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around USP5 on UCSC Golden Path with GeneCards custom track

Promoters for USP5 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around USP5 on UCSC Golden Path with GeneCards custom track

Genomic Location for USP5 Gene

Chromosome:
12
Start:
6,852,118 bp from pter
End:
6,866,632 bp from pter
Size:
14,515 bases
Orientation:
Plus strand

Genomic View for USP5 Gene

Genes around USP5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
USP5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for USP5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP5 Gene

Proteins for USP5 Gene

  • Protein details for USP5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P45974-UBP5_HUMAN
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 5
    Protein Accession:
    P45974
    Secondary Accessions:
    • D3DUS7
    • D3DUS8
    • Q96J22

    Protein attributes for USP5 Gene

    Size:
    858 amino acids
    Molecular mass:
    95786 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Interacts with TRIML1.
    Miscellaneous:
    • The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer.

    Three dimensional structures from OCA and Proteopedia for USP5 Gene

    Alternative splice isoforms for USP5 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for USP5 Gene

Proteomics data for USP5 Gene at MOPED

Post-translational modifications for USP5 Gene

  • Ubiquitination at Lys 163, Lys 318, Lys 353, Lys 357, Lys 360, Lys 391, Lys 423, Lys 553, Lys 586, Lys 743, Lys 793, and Lys 836
  • Modification sites at PhosphoSitePlus

Other Protein References for USP5 Gene

Domains & Families for USP5 Gene

Gene Families for USP5 Gene

Suggested Antigen Peptide Sequences for USP5 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P45974

UniProtKB/Swiss-Prot:

UBP5_HUMAN :
  • The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
  • Belongs to the peptidase C19 family.
  • Contains 1 UBP-type zinc finger.
Domain:
  • The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
  • Contains 2 UBA domains.
  • Contains 1 USP domain.
Family:
  • Belongs to the peptidase C19 family.
Similarity:
  • Contains 1 UBP-type zinc finger.
genes like me logo Genes that share domains with USP5: view

Function for USP5 Gene

Molecular function for USP5 Gene

GENATLAS Biochemistry:
ubiquitin specific protease 5,100kDa,expressed in brain,deubiquitin enzyme family
UniProtKB/Swiss-Prot CatalyticActivity:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
UniProtKB/Swiss-Prot Function:
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored Lys-48-linked polyubiquitin disassembly. Binds linear and Lys-63-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Enzyme Numbers (IUBMB) for USP5 Gene

Gene Ontology (GO) - Molecular Function for USP5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity TAS 7578059
GO:0004843 thiol-dependent ubiquitin-specific protease activity IDA 24424410
GO:0008242 omega peptidase activity IEA --
GO:0043130 ubiquitin binding IDA 24424410
genes like me logo Genes that share ontologies with USP5: view
genes like me logo Genes that share phenotypes with USP5: view

Animal Models for USP5 Gene

MGI Knock Outs for USP5:
  • Usp5 tm1a(EUCOMM)Hmgu

Animal Model Products

  • Taconic Biosciences Mouse Models for USP5

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for USP5 Gene

Localization for USP5 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for USP5 Gene COMPARTMENTS Subcellular localization image for USP5 gene
Compartment Confidence
lysosome 4
vacuole 4
cytosol 3
nucleus 3
cytoskeleton 2
peroxisome 1

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Gene Ontology (GO) - Cellular Components for USP5 Gene

Pathways & Interactions for USP5 Gene

genes like me logo Genes that share pathways with USP5: view

Pathways by source for USP5 Gene

1 Qiagen pathway for USP5 Gene

Gene Ontology (GO) - Biological Process for USP5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0071108 protein K48-linked deubiquitination IMP 19098288
genes like me logo Genes that share ontologies with USP5: view

No data available for SIGNOR curated interactions for USP5 Gene

Drugs & Compounds for USP5 Gene

(3) Drugs for USP5 Gene - From: ApexBio and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
WP1130 Pharma Deubiquitinase (DUB) inhibitor, Cell permeable 0

(1) Additional Compounds for USP5 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Water
  • Dihydrogen oxide
  • Steam
7732-18-5

(1) ApexBio Compounds for USP5 Gene

Compound Action Cas Number
WP1130 Deubiquitinase (DUB) inhibitor, Cell permeable 856243-80-6
genes like me logo Genes that share compounds with USP5: view

Transcripts for USP5 Gene

mRNA/cDNA for USP5 Gene

(2) REFSEQ mRNAs :
(6) Additional mRNA sequences :
(9) Selected AceView cDNA sequences:
(7) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for USP5 Gene

Ubiquitin specific peptidase 5 (isopeptidase T):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for USP5 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21a · 21b · 21c
SP1:
SP2: -
SP3:

Relevant External Links for USP5 Gene

GeneLoc Exon Structure for
USP5
ECgene alternative splicing isoforms for
USP5

Expression for USP5 Gene

mRNA expression in normal human tissues for USP5 Gene

Protein differential expression in normal tissues from HIPED for USP5 Gene

This gene is overexpressed in Lymph node (8.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for USP5 Gene



SOURCE GeneReport for Unigene cluster for USP5 Gene Hs.631661

genes like me logo Genes that share expression patterns with USP5: view

Protein tissue co-expression partners for USP5 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for USP5 Gene

Orthologs for USP5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for USP5 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia USP5 35
  • 92.42 (n)
  • 98.02 (a)
USP5 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia USP5 35
  • 93.67 (n)
  • 98.6 (a)
USP5 36
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Usp5 35
  • 91.96 (n)
  • 98.37 (a)
Usp5 16
Usp5 36
  • 98 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia USP5 35
  • 98.75 (n)
  • 98.93 (a)
USP5 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Usp5 35
  • 91.65 (n)
  • 98.25 (a)
oppossum
(Monodelphis domestica)
Mammalia USP5 36
  • 92 (a)
OneToOne
chicken
(Gallus gallus)
Aves USP5 35
  • 81.33 (n)
  • 91.35 (a)
USP5 36
  • 91 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia USP5 36
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4101 35
usp5 35
  • 73.92 (n)
  • 79.69 (a)
zebrafish
(Danio rerio)
Actinopterygii usp5 35
  • 66.87 (n)
  • 70.22 (a)
usp5 36
  • 69 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG12082 37
  • 50 (a)
CG12082 35
  • 58.62 (n)
  • 53.61 (a)
CG12082 36
  • 52 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012248 35
  • 58.94 (n)
  • 54.25 (a)
worm
(Caenorhabditis elegans)
Secernentea T27A3.2 37
  • 35 (a)
usp-5 36
  • 37 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBP14 36
  • 29 (a)
OneToMany
UBP14 38
thale cress
(Arabidopsis thaliana)
eudicotyledons UBP14 35
  • 55.85 (n)
  • 52.04 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.1989 35
rice
(Oryza sativa)
Liliopsida Os.7173 35
Os01g0177200 35
  • 55.39 (n)
  • 52.36 (a)
corn
(Zea mays)
Liliopsida Zm.5738 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9810 36
  • 49 (a)
OneToMany
Species with no ortholog for USP5:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for USP5 Gene

ENSEMBL:
Gene Tree for USP5 (if available)
TreeFam:
Gene Tree for USP5 (if available)

Paralogs for USP5 Gene

Paralogs for USP5 Gene

(1) SIMAP similar genes for USP5 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for USP5 Gene

genes like me logo Genes that share paralogs with USP5: view

Variants for USP5 Gene

Sequence variations from dbSNP and Humsavar for USP5 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs2071059 -- 6,860,567(+) CCTCT(A/G)TCAGC intron-variant
rs2071060 -- 6,864,473(+) TCACG(A/C)GGTCA intron-variant
rs2071061 -- 6,864,487(+) GATCA(A/G)GACCA intron-variant
rs2071062 -- 6,864,557(+) GGTGG(C/T)GGGCG intron-variant
rs2071063 -- 6,864,602(+) GAGAA(A/T)GGCGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for USP5 Gene

Variant ID Type Subtype PubMed ID
nsv832323 CNV Loss 17160897
nsv832324 CNV Loss 17160897
dgv1353n71 CNV Loss 21882294
nsv898641 CNV Loss 21882294
nsv898642 CNV Loss 21882294

Variation tolerance for USP5 Gene

Residual Variation Intolerance Score: 2.39% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.50; 29.19% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for USP5 Gene

HapMap Linkage Disequilibrium report
USP5

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for USP5 Gene

Disorders for USP5 Gene

Relevant External Links for USP5

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
USP5

No disorders were found for USP5 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for USP5 Gene

Publications for USP5 Gene

  1. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13. (PMID: 8723724) Ansari-Lari M.A. … Gibbs R.A. (Genome Res. 1996) 2 3 4 23 67
  2. Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53. (PMID: 19098288) Dayal S. … Saville M.K. (J. Biol. Chem. 2009) 3 23
  3. Recognition of polyubiquitin isoforms by the multiple ubiquitin binding modules of isopeptidase T. (PMID: 18482987) Reyes-Turcu F.E. … Wilkinson K.D. (J. Biol. Chem. 2008) 3 23
  4. The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. (PMID: 16564012) Reyes-Turcu F.E. … Wilkinson K.D. (Cell 2006) 3 23
  5. Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues. (PMID: 15096053) Russell N.S. … Wilkinson K.D. (Biochemistry 2004) 3 23

Products for USP5 Gene

Sources for USP5 Gene

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