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USP28 Gene

protein-coding   GIFtS: 57
GCID: GC11M113668

Ubiquitin Specific Peptidase 28

(Previous name: ubiquitin specific protease 28)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ubiquitin Specific Peptidase 281 2     Ubiquitin Carboxyl-Terminal Hydrolase 282
Ubiquitin Specific Protease 281 2     Ubiquitin Carboxyl-Terminal Hydrolase 28 Variant 12
Deubiquitinating Enzyme 282 3     Ubiquitin Thiolesterase 282
Ubiquitin Thioesterase 282 3     EC 3.4.19.123
Ubiquitin-Specific-Processing Protease 282 3     EC 3.1.2.158
KIAA15153 5     

External Ids:    HGNC: 126251   Entrez Gene: 576462   Ensembl: ENSG000000480287   OMIM: 6107485   UniProtKB: Q96RU23   

Export aliases for USP28 gene to outside databases

Previous GC identifers: GC11M116021 GC11M115180 GC11M113702 GC11M113206 GC11M113173 GC11M109597


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for USP28 Gene:
The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and
peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and
degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et
al., 2001).(supplied by OMIM, Mar 2008)

GeneCards Summary for USP28 Gene:
USP28 (ubiquitin specific peptidase 28) is a protein-coding gene. GO annotations related to this gene include ubiquitin-specific protease activity and ubiquitin thiolesterase activity. An important paralog of this gene is USP25.

UniProtKB/Swiss-Prot: UBP28_HUMAN, Q96RU2
Function: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in
DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN.
Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the
anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically
deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by
specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination
of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the
nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NT_033899.9  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the USP28 gene promoter:
         Max1   USF1   p53   Nkx2-5   LCR-F1   GATA-1   N-Myc   S8   USF-1   c-Myc   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for USP28

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat USP28


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q23   Ensembl cytogenetic band:  11q23.2   HGNC cytogenetic band: 11q23

USP28 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
USP28 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M113668:  view genomic region     (about GC identifiers)

Start:
113,668,596 bp from pter      End:
113,746,292 bp from pter
Size:
77,697 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: UBP28_HUMAN, Q96RU2 (See protein sequence)
Recommended Name: Ubiquitin carboxyl-terminal hydrolase 28  
Size: 1077 amino acids; 122491 Da
Subunit: Interacts with isoform 1 of FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of
MYC
1 PDB 3D structure from and Proteopedia for USP28:
2LVA (3D)    
Secondary accessions: B0YJC0 B0YJC1 Q9P213
Alternative splicing: 2 isoforms:  Q96RU2-1   Q96RU2-2   

Explore the universe of human proteins at neXtProt for USP28: NX_Q96RU2

Explore proteomics data for USP28 at MOPED

Post-translational modifications: 

  • Degradaded upon nickel ion level or hypoxia exposure1
  • Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR1
  • Ubiquitination2 at Lys243
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for USP28 (Q96RU2) (see all 5)
     LQYLFAL  LHAVLVH  FGQYPLQVNG  LPPVLTFELSRFEFNQ 


    See USP28 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_065937.1  
    ENSEMBL proteins: 
     ENSP00000003302   ENSP00000442431   ENSP00000444991   ENSP00000441513   ENSP00000440260  
     ENSP00000442257   ENSP00000445743   ENSP00000438050   ENSP00000437688   ENSP00000445722  
     ENSP00000440799   ENSP00000446284   ENSP00000437450   ENSP00000260188  

    USP28 Human Recombinant Protein Products:

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    Novus Biologicals USP28 Proteins
    Novus Biologicals USP28 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for USP28

     
    Search eBioscience for Proteins for USP28 

     
    antibodies-online proteins for USP28 (4 products) 

     
    antibodies-online peptides for USP28

    USP28 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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    antibodies-online antibodies for USP28 (46 products) 

    USP28 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for USP28
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for USP28
    Cloud-Clone Corp. CLIAs for USP28
    Search eBioscience for ELISAs for USP28 
    antibodies-online kits for USP28 (8 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    USP: Ubiquitin-specific peptidases

    5 InterPro protein domains:
     IPR001394 Peptidase_C19_UCH
     IPR018200 Pept_C19ubi-hydrolase_C_CS
     IPR028889 UCH/PAN2
     IPR003903 Ubiquitin-int_motif
     IPR009060 UBA-like

    Graphical View of Domain Structure for InterPro Entry Q96RU2

    ProtoNet protein and cluster: Q96RU2

    2 Blocks protein domains:
    IPB001394 Ubiquitin thiolesterase
    IPB003903 Ubiquitin interacting motif


    UniProtKB/Swiss-Prot: UBP28_HUMAN, Q96RU2
    Similarity: Belongs to the peptidase C19 family. USP28 subfamily
    Similarity: Contains 1 UIM (ubiquitin-interacting motif) repeat
    Similarity: Contains 1 USP domain


    Find genes that share domains with USP28           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: UBP28_HUMAN, Q96RU2
    Function: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in
    DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN.
    Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the
    anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically
    deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by
    specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination
    of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the
    nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus
    Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by
    the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)
    Induction: Down-regulated upon hypoxia

         Enzyme Numbers (IUBMB): EC 3.1.2.152 EC 3.4.19.121

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004221ubiquitin thiolesterase activity IMP16901786
    GO:0004843ubiquitin-specific protease activity IDA16901786
    GO:0005515protein binding IPI16901786
    GO:0008234cysteine-type peptidase activity ----
         
    Find genes that share ontologies with USP28           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for USP28:
     Increased resistance to MDM2 i 

         1 MGI phenotypic allele for Usp28 (no phenotypes)

    Find genes that share phenotypes with USP28           About GenesLikeMe

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for USP28

    miRNA
    Products:
        
    miRTarBase miRNAs that target USP28:
    hsa-mir-181a-5p (MIRT025164), hsa-mir-221-3p (MIRT046845), hsa-mir-615-3p (MIRT039972), hsa-mir-1301-3p (MIRT036013), hsa-mir-122-5p (MIRT023273)

    Block miRNA regulation of human, mouse, rat USP28 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate USP28 (see all 39):
    hsa-miR-26a-2* hsa-miR-106a hsa-miR-25 hsa-miR-301a hsa-miR-519a hsa-miR-29b-1* hsa-miR-93 hsa-miR-3613-3p
    SwitchGear 3'UTR luciferase reporter plasmidUSP28 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for USP28
    Predesigned siRNA for gene silencing in human, mouse, rat USP28

    Gene Editing
    Products:
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    Clone
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    OriGene clones in human, mouse for USP28 (see all 7)
    OriGene ORF clones in mouse, rat for USP28
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: USP28 (NM_020886)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for USP28
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP28
    Addgene plasmids for USP28 

    Cell Line
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP28


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    UBP28_HUMAN, Q96RU2: Nucleus, nucleoplasm
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm IDA17558397
    GO:0005730NOT nucleolus IDA--
    GO:0043234protein complex IDA17873522

    Find genes that share ontologies with USP28           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for USP28 About    
    See pathways by source

    SuperPathContained pathways About
    1Ubiquitin-Proteasome Dependent Proteolysis
    Ubiquitin-Proteasome Dependent Proteolysis
    2Protein Stability
    Protein Stability


    Find genes that share SuperPaths with USP28           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for USP28
        Ubiquitin-Proteasome Dependent Proteolysis

    1 Cell Signaling Technology (CST) Pathway for USP28
        Protein Stability


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for USP28
    Interactions:

        GeneGlobe Interaction Network for USP28

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for USP28 (Q96RU22, 3 ENSP000000033024) via UniProtKB, MINT, STRING, and/or I2D (see all 106)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ENSG00000206481Q146763, ENSP000003730604I2D: score=2 STRING: ENSP00000373060
    MDC1Q146763, ENSP000003655884I2D: score=2 STRING: ENSP00000365588
    ENSG00000224587Q146763I2D: score=2 
    ENSG00000225589Q146763I2D: score=2 
    ENSG00000228575Q146763I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000077DNA damage checkpoint IMP18662541
    GO:0006281DNA repair IEA--
    GO:0006511ubiquitin-dependent protein catabolic process IEA--
    GO:0006974cellular response to DNA damage stimulus IDA17873522
    GO:0008283cell proliferation IMP17558397

    Find genes that share ontologies with USP28           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for USP28 (UBP28)

    1 HMDB Compound for USP28    About this table
    CompoundSynonyms CAS #PubMed Ids
    WaterDihydrogen oxide (see all 2)7732-18-5--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for USP28 gene: 
    NM_020886.2  

    Unigene Cluster for USP28:

    Ubiquitin specific peptidase 28
    Hs.503891  [show with all ESTs]
    Unigene Representative Sequence: NM_020886
    16 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000003302(uc001pog.3 uc001poh.3 uc010rwy.2 uc001poi.3)
    ENST00000544967 ENST00000545540 ENST00000544272 ENST00000540438(uc010rwz.1)
    ENST00000538224 ENST00000538475 ENST00000537706(uc001poj.3) ENST00000545608
    ENST00000535607 ENST00000537490 ENST00000537642 ENST00000542033 ENST00000540925
    ENST00000544750 ENST00000260188
    miRNA
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    Block miRNA regulation of human, mouse, rat USP28 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate USP28 (see all 39):
    hsa-miR-26a-2* hsa-miR-106a hsa-miR-25 hsa-miR-301a hsa-miR-519a hsa-miR-29b-1* hsa-miR-93 hsa-miR-3613-3p
    SwitchGear 3'UTR luciferase reporter plasmidUSP28 3' UTR sequence
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    GenScript: all cDNA clones in your preferred vector: USP28 (NM_020886)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for USP28
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP28
    Addgene plasmids for USP28 
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for USP28
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat USP28
      QuantiTect SYBR Green Assays in human, mouse, rat USP28
      QuantiFast Probe-based Assays in human, mouse, rat USP28

    Additional mRNA sequence: 

    AB040948.1 AF266283.1 AK027521.1 AK097623.1 AK123255.1 AK304376.1 AK304770.1 BC035986.1 
    BC065928.1 

    11 DOTS entries:

    DT.40196816  DT.436446  DT.100020054  DT.99940140  DT.100020055  DT.101962320  DT.100020052  DT.101964560 
    DT.120727912  DT.40310650  DT.100671162 

    Selected AceView cDNA sequences (see all 100):

    AA490857 NM_020886 AI368173 AA348047 BQ421081 AW025713 AI273067 BG289257 
    AF266283 BE672125 AK097623 BX951921 AB040948 AW769730 CA448327 AW316631 
    BC065928 AI923030 AW515403 BU187516 W49570 BX503219 BM746208 C04935 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for USP28 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a ·
    SP1:                    -     -                                   -                                   -                       -                       -         
    SP2:                    -     -                                                                       -                             -     -     -     -     -   
    SP3:                    -     -                                   -                                   -                       -                                 
    SP4:                                                                                                                          -                       -         
    SP5:                                                                                                  -                       -     -                           

    ExUns: 19b ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^ 26 ^ 27 ^ 28a · 28b · 28c
    SP1:                                                                        
    SP2:                    -                                                   
    SP3:                                                                        
    SP4:                    -                                                   
    SP5:                                                                        


    ECgene alternative splicing isoforms for USP28

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    USP28 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    USP28 Expression
    About this image


    USP28 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 9) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    USP28 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    USP28 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.503891
        Custom PCR Arrays for USP28
    Primer
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    In Situ
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP28

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for USP28 gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Usp281 , 5 ubiquitin specific peptidase 281, 5 87.02(n)1
    90.72(a)1
      9 (26.56 cM)5
    2353231  NM_175482.31  NP_780691.21 
     489853855 
    chicken
    (Gallus gallus)
    Aves USP281 ubiquitin specific peptidase 28 69.09(n)
    68.9(a)
      428246  NM_001031496.1  NP_001026667.1 
    lizard
    (Anolis carolinensis)
    Reptilia USP286
    ubiquitin specific peptidase 28
    58(a)
    1 ↔ 1
    GL343354.1(235562-260196)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.120442 Transcribed sequence with moderate similarity to protein more 76.22(n)    BX703329.1 
    zebrafish
    (Danio rerio)
    Actinopterygii usp281 ubiquitin specific peptidase 28 61.73(n)
    60.16(a)
      567050  XM_001920061.3  XP_001920096.1 
    worm
    (Caenorhabditis elegans)
    Secernentea K02C4.36
    Protein K02C4.3 (K02C4.3) mRNA, complete cds
    17(a)
    1 → many
    II(8080789-8086849) WBGene00010502


    ENSEMBL Gene Tree for USP28 (if available)
    TreeFam Gene Tree for USP28 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for USP28 gene
    USP252  
    1 SIMAP similar gene for USP28 using alignment to 14 protein entries:     UBP28_HUMAN (see all proteins):
    USP25

    Find genes that share paralogs with USP28           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for USP28 (see all 1629)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1411345271,2
    --113668240(+) TTTTGA/GTATAT 1 -- int10--------
    rs454802911,2
    C,F--113668314(+) GAAAAT/CTGCTG 1 -- int14Minor allele frequency- C:0.11NS WA NA EA 396
    rs1447246361,2
    --113668437(+) CTTTTA/GTTTCA 1 -- int10--------
    rs1386780251,2
    --113668452(+) CTGGCA/GCGTCA 1 -- int10--------
    rs735529401,2
    C--113668752(+) TCTACA/CTATAC 1 -- ut312Minor allele frequency- C:0.10WA 120
    rs1397478961,2
    C--113668757(+) TATAC-/ATATATA 1 -- ut310--------
    rs2015737191,2
    --113668783(+) ATGTA-/TGCTGGTT 1 -- cds10--------
    rs30745601,2
    C--113668802(+) TTTTT-/T/TTTT
    T
    /TTTTTT
    TTTTT
    3 -- ut311NA 2
    rs124168541,2
    C,F,H--113668857(+) AAGGCC/GGGGTA 1 -- ut31 ese36Minor allele frequency- G:0.14NS EA WA 530
    rs1402822711,2
    C--113668946(+) AGCCTC/GGCCAA 1 -- ut310--------

    HapMap Linkage Disequilibrium report for USP28 (113668596 - 113746292 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for USP28:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv497CNV Insertion18451855
    nsv832273CNV Loss17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing USP28
    DNA2.0 Custom Variant and Variant Library Synthesis for USP28

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 610748    OMIM disorders: --


    Find genes that share disorders with USP28           About GenesLikeMe


    Export disorders for USP28 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for USP28 gene, integrated from 10 sources (see all 32):
    (articles sorted by number of sources associating them with USP28)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of alternatively spliced products and tissue- specific isoforms of USP28 and USP25. (PubMed id 11597335)1, 2, 3, 9 Valero R.... Marfany G. (Genome Biol. 2001)
    2. The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint. (PubMed id 18662541)1, 2 Bassermann F.... Pagano M. (Cell 2008)
    3. The ubiquitin-specific protease USP28 is required for MYC stability. (PubMed id 17558397)1, 2 Popov N.... Eilers M. (Nat. Cell Biol. 2007)
    4. Fbw7 and Usp28 regulate myc protein stability in response to DNA damage. (PubMed id 17873522)1, 2 Popov N.... Eilers M. (Cell Cycle 2007)
    5. A role for the deubiquitinating enzyme USP28 in control of the DNA- damage response. (PubMed id 16901786)1, 2 Zhang D.... Elledge S.J. (Cell 2006)
    6. Human and mouse proteases: a comparative genomic approach. (PubMed id 12838346)1, 3 Puente X.S.... Lopez-Otin C. (Nat. Rev. Genet. 2003)
    7. Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10819331)1, 2 Nagase T.... Ohara O. (DNA Res. 2000)
    8. A c-Myc-MicroRNA functional feedback loop affects hepatocarcinogenesis. (PubMed id 23389829)1 Han H....Li Y. (Hepatology 2013)
    9. The E3 ligase PIRH2 polyubiquitylates CHK2 and regulates its turnover. (PubMed id 23449389)1 Bohgaki M....Hakem R. (Cell Death Differ. 2013)
    10. Reactive oxygen species-dependent down-regulation of tumor suppressor genes PTEN, USP28, DRAM, TIGAR, and CYLD under oxidative stress. (PubMed id 23832602)1 Kim S.J....Lim C.J. (Biochem. Genet. 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 57646 HGNC: 12625 AceView: USP28 Ensembl:ENSG00000048028 euGenes: HUgn57646
    ECgene: USP28 H-InvDB: USP28

    (According to HUGE)
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    HUGE: KIAA1515

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for USP28 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for USP28 gene:
    Search GeneIP for patents involving USP28

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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