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Aliases for USP25 Gene

Aliases for USP25 Gene

  • Ubiquitin Specific Peptidase 25 2 3
  • Deubiquitinating Enzyme 25 3 4
  • Ubiquitin Thioesterase 25 3 4
  • USP On Chromosome 21 3 4
  • USP21 3 4
  • Ubiquitin-Specific Processing Protease 25 3
  • Ubiquitin-Specific-Processing Protease 25 4
  • Ubiquitin Carboxyl-Terminal Hydrolase 25 3
  • Ubiquitin Specific Protease 25 2
  • Ubiquitin Thiolesterase 25 3
  • EC 3.4.19.12 4
  • EC 3.1.2.15 63

External Ids for USP25 Gene

Previous GeneCards Identifiers for USP25 Gene

  • GC21P013766
  • GC21P016024
  • GC21P017102
  • GC21P002481

Summaries for USP25 Gene

Entrez Gene Summary for USP25 Gene

  • Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

GeneCards Summary for USP25 Gene

USP25 (Ubiquitin Specific Peptidase 25) is a Protein Coding gene. Among its related pathways are Integrated Breast Cancer Pathway and Ubiquitin-Proteasome Dependent Proteolysis. GO annotations related to this gene include ubiquitin thiolesterase activity and ubiquitin-specific protease activity. An important paralog of this gene is USP28.

UniProtKB/Swiss-Prot for USP25 Gene

  • Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains

  • The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for USP25 Gene

Genomics for USP25 Gene

Regulatory Elements for USP25 Gene

Genomic Location for USP25 Gene

Start:
15,730,008 bp from pter
End:
15,880,072 bp from pter
Size:
150,065 bases
Orientation:
Plus strand

Genomic View for USP25 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for USP25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP25 Gene

Proteins for USP25 Gene

  • Protein details for USP25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UHP3-UBP25_HUMAN
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 25
    Protein Accession:
    Q9UHP3
    Secondary Accessions:
    • C0LSZ0
    • Q6DHZ9
    • Q9H9W1

    Protein attributes for USP25 Gene

    Size:
    1055 amino acids
    Molecular mass:
    122218 Da
    Quaternary structure:
    • Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.

    Alternative splice isoforms for USP25 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for USP25 Gene

Proteomics data for USP25 Gene at MOPED

Selected DME Specific Peptides for USP25 Gene

Post-translational modifications for USP25 Gene

  • Acetylated.
  • Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
  • Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
  • Ubiquitination at Lys15, Lys201, Lys250, Lys254, Lys312, Lys313, Lys392, Lys444, Lys520, Lys584, Lys630, Lys698, Lys715, and Lys721
  • Modification sites at PhosphoSitePlus

Domains for USP25 Gene

Gene Families for USP25 Gene

HGNC:
  • USP :Ubiquitin-specific peptidases

Suggested Antigen Peptide Sequences for USP25 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9UHP3

UniProtKB/Swiss-Prot:

UBP25_HUMAN :
  • Q9UHP3
Domain:
  • Contains 1 UBA-like domain.
  • Contains 2 UIM (ubiquitin-interacting motif) domains.
  • Contains 1 USP domain.
Family:
  • Belongs to the peptidase C19 family.
genes like me logo Genes that share domains with USP25: view

Function for USP25 Gene

Molecular function for USP25 Gene

GENATLAS Biochemistry: ubiquitin specific protease 25,expressed in testis,pancreas,kidney,liver,lung,brain,heart,skeletal muscle,encoding two alternatively spliced isoforms,deubiquitin enzyme family
UniProtKB/Swiss-Prot CatalyticActivity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)
UniProtKB/Swiss-Prot Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains
UniProtKB/Swiss-Prot Function: The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function
UniProtKB/Swiss-Prot Induction: The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to 100-fold towards completion of differentiation.

Enzyme Numbers (IUBMB) for USP25 Gene

Gene Ontology (GO) - Molecular Function for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity IBA --
GO:0004843 ubiquitin-specific protease activity IMP 18538659
GO:0005515 protein binding IPI 18538659
GO:0008233 peptidase activity TAS 10644437
GO:0032183 SUMO binding IDA 18538659
genes like me logo Genes that share ontologies with USP25: view
genes like me logo Genes that share phenotypes with USP25: view

miRNA for USP25 Gene

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for USP25 Gene

Localization for USP25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for USP25 Gene

Cytoplasm.
Isoform USP25m: Cytoplasm. Nucleus. Note=Some transient punctuate nuclear location in myotubes during myocyte development. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for USP25 Gene COMPARTMENTS Subcellular localization image for USP25 gene
Compartment Confidence
cytosol 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA --
GO:0005737 cytoplasm IDA --
genes like me logo Genes that share ontologies with USP25: view

Pathways for USP25 Gene

genes like me logo Genes that share pathways with USP25: view

Pathways by source for USP25 Gene

1 Qiagen pathway for USP25 Gene
1 BioSystems pathway for USP25 Gene

Gene Ontology (GO) - Biological Process for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006464 cellular protein modification process TAS 10644437
GO:0006508 proteolysis TAS 10644437
GO:0006511 ubiquitin-dependent protein catabolic process --
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process IBA --
GO:0061136 regulation of proteasomal protein catabolic process IBA --
genes like me logo Genes that share ontologies with USP25: view

Compounds for USP25 Gene

(1) HMDB Compounds for USP25 Gene

Compound Synonyms Cas Number PubMed IDs
Water
  • Dihydrogen oxide
7732-18-5
genes like me logo Genes that share compounds with USP25: view

Transcripts for USP25 Gene

Unigene Clusters for USP25 Gene

Ubiquitin specific peptidase 25:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for USP25 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^
SP1: - -
SP2: - - -
SP3: - - - - - - - - - - - - - - - - - -
SP4:
SP5: -
SP6: -
SP7: -
SP8:
SP9:

ExUns: 21 ^ 22 ^ 23 ^ 24 ^ 25a · 25b ^ 26a · 26b
SP1: -
SP2: -
SP3: - - -
SP4: -
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for USP25 Gene

GeneLoc Exon Structure for
USP25
ECgene alternative splicing isoforms for
USP25

Expression for USP25 Gene

mRNA expression in normal human tissues for USP25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for USP25 Gene

This gene is overexpressed in Muscle - Skeletal (4.7).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for USP25 Gene

SOURCE GeneReport for Unigene cluster for USP25 Gene Hs.743994

mRNA Expression by UniProt/SwissProt for USP25 Gene

Q9UHP3-UBP25_HUMAN
Tissue specificity: Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
genes like me logo Genes that share expressions with USP25: view

Orthologs for USP25 Gene

This gene was present in the common ancestor of animals.

Orthologs for USP25 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia USP25 35
  • 99.69 (n)
  • 99.63 (a)
USP25 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia USP25 35
  • 93.71 (n)
  • 97.06 (a)
USP25 36
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia USP25 35
  • 94.6 (n)
  • 97.6 (a)
USP25 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Usp25 35
  • 86.9 (n)
  • 92.57 (a)
Usp25 16
Usp25 36
  • 94 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia USP25 36
  • 80 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia USP25 36
  • 81 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Usp25 35
  • 87.79 (n)
  • 92.94 (a)
chicken
(Gallus gallus)
Aves USP25 35
  • 81.97 (n)
  • 85.92 (a)
USP25 36
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia USP25 36
  • 65 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.18074 35
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4178 35
usp25 35
  • 74.99 (n)
  • 81.96 (a)
zebrafish
(Danio rerio)
Actinopterygii Dr.12045 35
usp25 35
  • 68.32 (n)
  • 76.64 (a)
usp25 36
  • 77 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea K02C4.3 36
  • 17 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 35 (a)
OneToMany
Species with no ortholog for USP25:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for USP25 Gene

ENSEMBL:
Gene Tree for USP25 (if available)
TreeFam:
Gene Tree for USP25 (if available)

Paralogs for USP25 Gene

Paralogs for USP25 Gene

Selected SIMAP similar genes for USP25 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with USP25: view

Variants for USP25 Gene

Sequence variations from dbSNP and Humsavar for USP25 Gene

SNP ID Clin Chr 21 pos Sequence Context AA Info Type MAF
rs456899 -- 15,751,536(+) TTTAA(C/T)TGGAC intron-variant
rs459438 -- 15,728,866(+) GGTAC(A/C)AGACA upstream-variant-2KB
rs459495 -- 15,761,690(+) tagga(A/G)gaatc intron-variant
rs459605 -- 15,763,445(+) CATTC(A/G)ATTTC intron-variant
rs461182 -- 15,765,473(+) GTTCT(A/G)TGTGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for USP25 Gene

Variant ID Type Subtype PubMed ID
nsv459099 CNV Gain 19166990
esv275197 CNV Gain+Loss 21479260
nsv834050 CNV Gain 17160897
nsv3461 CNV Insertion 18451855
nsv3462 CNV Insertion 18451855

Relevant External Links for USP25 Gene

HapMap Linkage Disequilibrium report
USP25

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for USP25 Gene

Disorders for USP25 Gene

Relevant External Links for USP25

Genetic Association Database (GAD)
USP25
Human Genome Epidemiology (HuGE) Navigator
USP25
genes like me logo Genes that share disorders with USP25: view

No data available for UniProtKB/Swiss-Prot for USP25 Gene

Publications for USP25 Gene

  1. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. (PMID: 10644437) Valero R. … Gonzalez-Duarte R. (Genomics 1999) 3 4 23
  2. Characterization of alternatively spliced products and tissue- specific isoforms of USP28 and USP25. (PMID: 11597335) Valero R. … Marfany G. (Genome Biol. 2001) 3 4 23
  3. The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins. (PMID: 16501887) Bosch-Comas A. … Marfany G. (Cell. Mol. Life Sci. 2006) 3 4 23
  4. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. (PMID: 18538659) Meulmeester E. … Melchior F. (Mol. Cell 2008) 3 4 23
  5. Functional interaction between the ubiquitin-specific protease 25 and the SYK tyrosine kinase. (PMID: 19909739) Cholay M. … Daviet L. (Exp. Cell Res. 2010) 3 4 23

Products for USP25 Gene

Sources for USP25 Gene

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