Free for academic non-profit institutions. Other users need a Commercial license

Aliases for USP25 Gene

Aliases for USP25 Gene

  • Ubiquitin Specific Peptidase 25 2 3 5
  • Deubiquitinating Enzyme 25 3 4
  • Ubiquitin Thioesterase 25 3 4
  • USP On Chromosome 21 3 4
  • USP21 3 4
  • Ubiquitin-Specific Processing Protease 25 3
  • Ubiquitin-Specific-Processing Protease 25 4
  • Ubiquitin Specific Protease 25 2
  • Ubiquitin Thiolesterase 25 3
  • EC 3.4.19.12 4
  • EC 3.1.2.15 63

External Ids for USP25 Gene

Previous GeneCards Identifiers for USP25 Gene

  • GC21P013766
  • GC21P016024
  • GC21P017102
  • GC21P002481

Summaries for USP25 Gene

Entrez Gene Summary for USP25 Gene

  • Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

GeneCards Summary for USP25 Gene

USP25 (Ubiquitin Specific Peptidase 25) is a Protein Coding gene. Among its related pathways are Ubiquitin-Proteasome Dependent Proteolysis. GO annotations related to this gene include peptidase activity and thiol-dependent ubiquitin-specific protease activity. An important paralog of this gene is USP28.

UniProtKB/Swiss-Prot for USP25 Gene

  • Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains.

  • The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for USP25 Gene

Genomics for USP25 Gene

Regulatory Elements for USP25 Gene

Enhancers for USP25 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around USP25 on UCSC Golden Path with GeneCards custom track

Promoters for USP25 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around USP25 on UCSC Golden Path with GeneCards custom track

Genomic Location for USP25 Gene

Chromosome:
21
Start:
15,729,947 bp from pter
End:
15,880,072 bp from pter
Size:
150,126 bases
Orientation:
Plus strand

Genomic View for USP25 Gene

Genes around USP25 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
USP25 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for USP25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP25 Gene

Proteins for USP25 Gene

  • Protein details for USP25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UHP3-UBP25_HUMAN
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 25
    Protein Accession:
    Q9UHP3
    Secondary Accessions:
    • C0LSZ0
    • Q6DHZ9
    • Q9H9W1

    Protein attributes for USP25 Gene

    Size:
    1055 amino acids
    Molecular mass:
    122218 Da
    Quaternary structure:
    • Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.

    Alternative splice isoforms for USP25 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for USP25 Gene

Proteomics data for USP25 Gene at MOPED

Selected DME Specific Peptides for USP25 Gene

Post-translational modifications for USP25 Gene

  • Acetylated.
  • Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
  • Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
  • Ubiquitination at Lys 15, Lys 201, Lys 250, Lys 254, Lys 312, Lys 313, Lys 392, Lys 444, Lys 520, Lys 584, Lys 630, Lys 698, Lys 715, and Lys 721
  • Modification sites at PhosphoSitePlus

Antibody Products

Domains & Families for USP25 Gene

Gene Families for USP25 Gene

Suggested Antigen Peptide Sequences for USP25 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9UHP3

UniProtKB/Swiss-Prot:

UBP25_HUMAN :
  • Contains 1 UBA-like domain.
  • Belongs to the peptidase C19 family.
Domain:
  • Contains 1 UBA-like domain.
  • Contains 2 UIM (ubiquitin-interacting motif) domains.
  • Contains 1 USP domain.
Family:
  • Belongs to the peptidase C19 family.
genes like me logo Genes that share domains with USP25: view

Function for USP25 Gene

Molecular function for USP25 Gene

GENATLAS Biochemistry:
ubiquitin specific protease 25,expressed in testis,pancreas,kidney,liver,lung,brain,heart,skeletal muscle,encoding two alternatively spliced isoforms,deubiquitin enzyme family
UniProtKB/Swiss-Prot CatalyticActivity:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
UniProtKB/Swiss-Prot Function:
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains.
UniProtKB/Swiss-Prot Function:
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
UniProtKB/Swiss-Prot Induction:
The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to 100-fold towards completion of differentiation.

Enzyme Numbers (IUBMB) for USP25 Gene

Gene Ontology (GO) - Molecular Function for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004843 thiol-dependent ubiquitin-specific protease activity IMP 18538659
GO:0008233 peptidase activity TAS 10644437
GO:1904455 ubiquitin-specific protease activity involved in negative regulation of ERAD pathway NAS 22590560
genes like me logo Genes that share ontologies with USP25: view
genes like me logo Genes that share phenotypes with USP25: view

Animal Model Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for USP25 Gene

Localization for USP25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for USP25 Gene

Cytoplasm.
Isoform USP25m: Cytoplasm. Nucleus. Note=Some transient punctuate nuclear location in myotubes during myocyte development. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for USP25 Gene COMPARTMENTS Subcellular localization image for USP25 gene
Compartment Confidence
endoplasmic reticulum 5
cytosol 4
nucleus 4
mitochondrion 2
peroxisome 1

No data available for Gene Ontology (GO) - Cellular Components for USP25 Gene

Pathways & Interactions for USP25 Gene

genes like me logo Genes that share pathways with USP25: view

Pathways by source for USP25 Gene

1 Qiagen pathway for USP25 Gene

Gene Ontology (GO) - Biological Process for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0071108 protein K48-linked deubiquitination IMP 18538659
GO:1904293 negative regulation of ERAD pathway IMP 22590560
genes like me logo Genes that share ontologies with USP25: view

No data available for SIGNOR curated interactions for USP25 Gene

Drugs & Compounds for USP25 Gene

(1) Additional Compounds for USP25 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Water
  • Dihydrogen oxide
  • Steam
7732-18-5
genes like me logo Genes that share compounds with USP25: view

Transcripts for USP25 Gene

Unigene Clusters for USP25 Gene

Ubiquitin specific peptidase 25:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for USP25 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^
SP1: - -
SP2: - - -
SP3: - - - - - - - - - - - - - - - - - -
SP4:
SP5: -
SP6: -
SP7: -
SP8:
SP9:

ExUns: 21 ^ 22 ^ 23 ^ 24 ^ 25a · 25b ^ 26a · 26b
SP1: -
SP2: -
SP3: - - -
SP4: -
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for USP25 Gene

GeneLoc Exon Structure for
USP25
ECgene alternative splicing isoforms for
USP25

Expression for USP25 Gene

mRNA expression in normal human tissues for USP25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for USP25 Gene

This gene is overexpressed in Muscle - Skeletal (x4.7).

Protein differential expression in normal tissues from HIPED for USP25 Gene

This gene is overexpressed in Lymph node (13.9), Monocytes (8.8), Plasma (7.6), and CD8 Tcells (6.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for USP25 Gene



SOURCE GeneReport for Unigene cluster for USP25 Gene Hs.743994

mRNA Expression by UniProt/SwissProt for USP25 Gene

Q9UHP3-UBP25_HUMAN
Tissue specificity: Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
genes like me logo Genes that share expression patterns with USP25: view

Primer Products

In Situ Assay Products

No data available for Protein tissue co-expression partners for USP25 Gene

Orthologs for USP25 Gene

This gene was present in the common ancestor of animals.

Orthologs for USP25 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia USP25 35
  • 93.71 (n)
  • 97.06 (a)
USP25 36
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia USP25 35
  • 94.6 (n)
  • 97.6 (a)
USP25 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Usp25 35
  • 86.9 (n)
  • 92.57 (a)
Usp25 16
Usp25 36
  • 94 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia USP25 35
  • 99.69 (n)
  • 99.63 (a)
USP25 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Usp25 35
  • 87.79 (n)
  • 92.94 (a)
oppossum
(Monodelphis domestica)
Mammalia USP25 36
  • 80 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia USP25 36
  • 81 (a)
OneToOne
chicken
(Gallus gallus)
Aves USP25 35
  • 81.97 (n)
  • 85.92 (a)
USP25 36
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia USP25 36
  • 65 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4178 35
usp25 35
  • 74.99 (n)
  • 81.96 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.18074 35
zebrafish
(Danio rerio)
Actinopterygii Dr.12045 35
usp25 35
  • 68.32 (n)
  • 76.64 (a)
usp25 36
  • 77 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea K02C4.3 36
  • 17 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 35 (a)
OneToMany
Species with no ortholog for USP25:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for USP25 Gene

ENSEMBL:
Gene Tree for USP25 (if available)
TreeFam:
Gene Tree for USP25 (if available)

Paralogs for USP25 Gene

Paralogs for USP25 Gene

(1) SIMAP similar genes for USP25 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with USP25: view

Variants for USP25 Gene

Sequence variations from dbSNP and Humsavar for USP25 Gene

SNP ID Clin Chr 21 pos Sequence Context AA Info Type
rs2252133 -- 15,874,699(+) TATTC(G/T)TTTCT intron-variant
rs2274797 -- 15,842,521(+) AAGTC(C/T)TGAAA intron-variant, reference, missense
rs2297247 -- 15,763,053(+) TTTTT(G/T)GGGTA intron-variant
rs2297248 -- 15,831,466(+) TGGGC(A/T)TATAT reference, synonymous-codon
rs2297249 -- 15,874,655(+) AACTG(A/G)TTCTA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for USP25 Gene

Variant ID Type Subtype PubMed ID
nsv459099 CNV Gain 19166990
esv275197 CNV Gain+Loss 21479260
nsv834050 CNV Gain 17160897
nsv3461 CNV Insertion 18451855
nsv3462 CNV Insertion 18451855

Variation tolerance for USP25 Gene

Residual Variation Intolerance Score: 6.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.46; 54.96% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for USP25 Gene

HapMap Linkage Disequilibrium report
USP25

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for USP25 Gene

Disorders for USP25 Gene

Relevant External Links for USP25

Genetic Association Database (GAD)
USP25
Human Genome Epidemiology (HuGE) Navigator
USP25
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
USP25

No disorders were found for USP25 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for USP25 Gene

Publications for USP25 Gene

  1. Narrowing of the region of allelic loss in 21q11-21 in squamous non- small cell lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the deleted segment. (PMID: 10612803) Groet J. … Nizetic D. (Genes Chromosomes Cancer 2000) 2 3 4 67
  2. Functional interaction between the ubiquitin-specific protease 25 and the SYK tyrosine kinase. (PMID: 19909739) Cholay M. … Daviet L. (Exp. Cell Res. 2010) 3 23
  3. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. (PMID: 18538659) Meulmeester E. … Melchior F. (Mol. Cell 2008) 3 23
  4. The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins. (PMID: 16501887) Bosch-Comas A. … Marfany G. (Cell. Mol. Life Sci. 2006) 3 23
  5. Human and mouse proteases: a comparative genomic approach. (PMID: 12838346) Puente X.S. … Lopez-Otin C. (Nat. Rev. Genet. 2003) 2 3

Products for USP25 Gene

Sources for USP25 Gene

Content