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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

USP25 Gene

protein-coding GIFtS: 57
GCID: GC21P017102

ubiquitin specific peptidase 25

(Previous name: ubiquitin specific protease 25 )

Explore 9 diseases affiliated with
USP25 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, and/or ¹¹Nature:405,311-319 and CroW21)
About This Section

Aliases
Ubiquitin Specific Peptidase 25¹ ²		Ubiquitin Specific Protease USP2511
USP21¹ ² ³		Ubiquitin Carboxyl-Terminal Hydrolase 25²
Ubiquitin Specific Protease 25¹ ²		Ubiquitin Thiolesterase 25²
Deubiquitinating Enzyme 25² ³		Ubiquitin-Specific Processing Protease 25²
Ubiquitin Thioesterase 25² ³		EC 3.4.19.12³
Ubiquitin-Specific-Processing Protease 25² ³		EC 3.1.2.15⁸
USP On Chromosome 21² ³

External Ids:	HGNC: 12624¹	Entrez Gene: 29761²	Ensembl: ENSG00000155313⁷	OMIM: 604736⁵	UniProtKB: Q9UHP3³

Export aliases for USP25 gene to outside databases

Previous GC identifers: GC21P013766 GC21P016024 GC21P002481

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for USP25:

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein

breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of

the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999

(PubMed 10644437)).(supplied by OMIM, Mar 2008)

UniProtKB/Swiss-Prot: UBP25_HUMAN, Q9UHP3

Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to

process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents

proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains

Function: The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and

function

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics, Whole Chromsome Sequence According to Nature (Cited Here with Permission):405,311-319 and CroW21)
About This Section

RefSeq DNA sequence:

NC_000021.8 NC_018932.1 NT_011512.11

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the USP25 gene promoter:
STAT5B STAT1 AML1a STAT4 STAT2 STAT3 STAT1beta STAT6 STAT5A STAT1alpha
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: USP25 promoter sequence

Search SABiosciences Chromatin IP Primers for USP25

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat USP25

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 21q11.2 Ensembl cytogenetic band: 21q21.1 HGNC cytogenetic band: 21q11.2
Nature(405: 311-319) cytogenetic band: 21q11

USP25 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
USP25 gene location

GeneLoc information about chromosome 21 GeneLoc Exon Structure

(about GC identifiers)

GC21P017102:	GeneLoc	Nature:405,311-319
Start:	17,102,344 bp from pter	2,766,805 bp from centromere
End:	17,252,377 bp from pter	2,915,540 bp from centromere
Size:	150,034 bases	148,736 bases
Orientation:	plus strand	plus strand

Whole chromosome sequencing:
cDNA sequence:	AF170562
genomic clones:	P111K10 to P135E14

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: UBP25_HUMAN, Q9UHP3 (See protein sequence)

Recommended Name: Ubiquitin carboxyl-terminal hydrolase 25

Size: 1055 amino acids; 122218 Da

Subunit: Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but

is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the

interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament

repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently

USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only

weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels

Subcellular location: Cytoplasm

Subcellular location: Isoform USP25m: Cytoplasm (By similarity). Nucleus (By similarity). Note=Some transient punctuate

nuclear location in myotubes during myocyte development (By similarity)

Secondary accessions: C0LSZ0 Q6DHZ9 Q9H9W1

Alternative splicing: 3 isoforms: Q9UHP3-2 Q9UHP3-1 Q9UHP3-3

Explore the universe of human proteins at neXtProt for USP25: NX_Q9UHP3

Post-translational modifications:

Acetylated¹

Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3.

Desumoylated by SENP1. Regulated by ubiquitination on the same residue¹

Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the

enzymatic activity either by preventing sumoylation or by allowing novel interactions¹

Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q9UHP3

4 DME Specific Peptides for USP25 (Q9UHP3)

LHAVLVH

FGQYPLQVNG

LPPVLTFELSRFEFNQ

QQQDVSEFTHKLLDWLEDAFQ

USP25 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_037528.3

ENSEMBL proteins:

ENSP00000285681 ENSP00000285679 ENSP00000299574 ENSP00000404086 ENSP00000402354

ENSP00000383044

Human Recombinant Protein Products:

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	R&D Systems Recombinant & Natural Proteins for USP25
	Enzo Life Sciences proteins for USP25
	OriGene Purified Protein: USP25 OriGene Protein Over-expression Lysate: USP25 OriGene Custom Protein Services for USP25
	GenScript Custom Purified and Recombinant Proteins Services for USP25
	Novus Biologicals USP25 Protein Novus Biologicals USP25 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for USP25

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005737	cytoplasm	IEA	--

USP25 for ontologies About GeneDecksing

USP25 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	Browse OriGene Antibodies OriGene Custom Antibody Services for USP25
	GenScript Custom Superior Antibodies Services for USP25
	Novus Biologicals USP25 Antibodies
	Search for Antibodies for USP25 at Abcam
	Uscn Antibodies for USP25
	ThermoFisher Antibodies for USP25

Assay Products for USP25:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for USP25
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for USP25

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

USP25 for domains About GeneDecksing

4 InterPro domains/families:

IPR001394 Peptidase_C19

IPR018200 Pept_C19ubi-hydrolase_C_CS

IPR003903 Ubiquitin-int_motif

IPR009060 UBA-like

Graphical View of Domain Structure for InterPro Entry Q9UHP3

ProtoNet protein and cluster: Q9UHP3

2 Blocks protein families:

IPB001394 Ubiquitin thiolesterase

IPB003903 Ubiquitin interacting motif

UniProtKB/Swiss-Prot: UBP25_HUMAN, Q9UHP3

Similarity: Belongs to the peptidase C19 family

Similarity: Contains 1 UBA-like domain

Similarity: Contains 2 UIM (ubiquitin-interacting motif) repeats

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: UBP25_HUMAN, Q9UHP3

Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to

process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents

proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains

Function: The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and

function

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the

C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)

Induction: The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to

100-fold towards completion of differentiation

Genatlas biochemistry entry for USP25:

ubiquitin specific protease 25,expressed in testis,pancreas,kidney,liver,lung,brain,heart,skeletal muscle,encoding two

alternatively spliced isoforms,deubiquitin enzyme family

Enzyme Numbers (IUBMB): EC 3.1.2.15² EC 3.4.19.12¹

miRNA Products:		OriGene 3'-UTR Clone: USP25 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat USP25
		8/60 QIAGEN miScript miRNA Assays for microRNAs that regulate USP25 (see all 60):
		hsa-miR-579 hsa-miR-548j hsa-miR-300 hsa-miR-429 hsa-miR-513a-5p hsa-miR-4272 hsa-miR-875-5p hsa-miR-15a

		SwitchGear 3'UTR luciferase reporter plasmid: USP25 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for USP25 (see all 7) OriGene shRNA RFP: USP25 OriGene siRNA: USP25
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat USP25

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for USP25

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for USP25 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for USP25 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: USP25 (NM_013396)
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		DNA2.0 Custom Codon Optimized Gene Synthesis Service for USP25
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP25

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for USP25
		Search LifeMap BioReagents cell lines for USP25

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP25

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004221	ubiquitin thiolesterase activity	IEA	--
GO:0004843	ubiquitin-specific protease activity	IMP	18538659
GO:0005515	protein binding	IPI	18538659
GO:0008233	peptidase activity	TAS	10644437
GO:0032183	SUMO binding	IDA	18538659

USP25 for ontologies About GeneDecksing

2 GenomeRNAi human phenotypes for USP25:

Increased cell death in HCT116

Synthetic lethal with Ras

Animal Models:
3 MGI mutant phenotypes (inferred from 1 allele) (MGI details for Usp25):

hematopoietic system

immune system

respiratory system

USP25 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Ubiquitin-Proteasome Dependent Proteolysis

Ubiquitin-Proteasome Dependent Proteolysis

1.00

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for USP25

Ubiquitin-Proteasome Dependent Proteolysis

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for USP25

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/31 Interacting proteins for USP25 (Q9UHP3^{2, 3} ENSP00000285679⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 31)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
SKIL	P12757²^,³	MINT-61673 I2D: score=3
BTBD9	Q96Q07³, ENSP00000386121⁴	I2D: score=2 STRING: ENSP00000386121
KCTD10	Q9H3F6³, ENSP00000228495⁴	I2D: score=2 STRING: ENSP00000228495
KCTD13	Q8WZ19³, ENSP00000311202⁴	I2D: score=2 STRING: ENSP00000311202
KLHL9	Q9P2J3³, ENSP00000351933⁴	I2D: score=2 STRING: ENSP00000351933

About this table

Gene Ontology (GO): 5 biological process terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006464	cellular protein modification process	TAS	10644437
GO:0006508	proteolysis	TAS	10644437
GO:0006511	ubiquitin-dependent protein catabolic process	IEA	--
GO:0070536	protein K63-linked deubiquitination	IMP	18538659
GO:0071108	protein K48-linked deubiquitination	IMP	18538659

USP25 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for USP25

1 HMDB Compound for USP25 About this table

Compound	Synonyms	CAS #	PubMed Ids
Water	Dihydrogen oxide (see all 2)	7732-18-5	--

Search CenterWatch for drugs/clinical trials and news about USP25 / UBP25

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for USP25 gene:

NM_013396.3

Unigene Cluster for USP25:

Ubiquitin specific peptidase 25

Hs.743994 [show with all ESTs]

Unigene Representative Sequence: AL833500

10 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000285681(uc002yjz.1) ENST00000285679 ENST00000351097 ENST00000547201

ENST00000549362 ENST00000464435 ENST00000453553 ENST00000449491 ENST00000478932

ENST00000400183(uc002yjy.1 uc011aby.1 uc010gla.1)

miRNA Products:		OriGene 3'-UTR Clone: USP25 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat USP25
		8/60 QIAGEN miScript miRNA Assays for microRNAs that regulate USP25 (see all 60):
		hsa-miR-579 hsa-miR-548j hsa-miR-300 hsa-miR-429 hsa-miR-513a-5p hsa-miR-4272 hsa-miR-875-5p hsa-miR-15a

		SwitchGear 3'UTR luciferase reporter plasmid: USP25 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for USP25 (see all 7) OriGene shRNA RFP: USP25 OriGene siRNA: USP25
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat USP25

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for USP25 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for USP25 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: USP25 (NM_013396)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP25

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for USP25 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat USP25
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat USP25
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat USP25

Additional cDNA sequence:

AF134213.1 AF170562.1 AF419247.1 AK022447.1 AK022574.1 AK094083.1 AL833500.1 BC015930.1

BC018686.1 BC075792.1 FJ763652.1

13 DOTS entries:

DT.113035 DT.95087262 DT.100024030 DT.100662230 DT.101961770 DT.97765322 DT.91763471 DT.95087288

DT.95228867 DT.91796381 DT.95087264 DT.121137346 DT.40298560

24/137 AceView cDNA sequences (see all 137):

AI168012 T36007 AA307805 BG567138 AI809934 AA385363 BM758824 AI538808

BU569569 AK022574 NM_013396 AW316869 BQ215557 AA382386 AA731841 BC015930

Z40738 CR600261 AA644617 AW264420 AL833500 AI284337 H01462 AI985497

GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for USP25 (see all 9) About this scheme

ExUns:	1	^	2	^	3	^	4	^	5a	·	5b	^	6	^	7	^	8	^	9a	·	9b	^	10a	·	10b	·	10c	^	11	^	12a	·	12b	^	13	^	14	^	15	^	16	^	17a	·	17b	^	18	^	19	^	20	^
SP1:																			-																																-
SP2:																			-																														-		-
SP3:																	-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-
SP4:
SP5:																																			-

ExUns:	21	^	22	^	23	^	24	^	25a	·	25b	^	26a	·	26b
SP1:									-
SP2:									-
SP3:	-		-						-
SP4:									-
SP5:

ECgene alternative splicing isoforms for USP25

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

USP25 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --

About this image
See USP25 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for USP25

SOURCE GeneReport for Unigene cluster: Hs.743994

UniProtKB/Swiss-Prot: UBP25_HUMAN, Q9UHP3

Tissue specificity: Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis,

pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small

intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal

muscle. Isoform USB25m is heart and skeletal muscle specific

SABiosciences Custom PCR Arrays for USP25

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for USP25 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat USP25
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat USP25
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat USP25

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP25

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for USP25 gene from 6/20 species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Usp25^{1 , 5}	ubiquitin specific peptidase 25^{1, 5}	87.86(n)¹ 94.02(a)¹		16 (44.40 cM)⁵ 30940¹ NM_013918.2¹ NP_038946.2¹ 77014069⁵
chicken (Gallus gallus)	Aves	USP25¹	ubiquitin specific peptidase 25	82.38(n) 87.22(a)		418471 XM_416680.3 XP_416680.2
lizard (Anolis carolinensis)	Reptilia	USP25⁶	--	66(a)	1 ↔ 1	3(154400131-154436588)
African clawed frog (Xenopus laevis)	Amphibia	Xl.18074²	Xenopus laevis transcribed sequence with weak similarity more	81.17(n)		CA790722.1
zebrafish (Danio rerio)	Actinopterygii	Dr.12045²	Danio rerio mRNA similar to ubiquitin specific protease more	75.72(n)		BC045454.1
worm (Caenorhabditis elegans)	Secernentea	K02C4.3⁶	Probable ubiquitin carboxyl-terminal hydrolase K02...	15(a)	1 → many	II(8080737-8086797)

ENSEMBL Gene Tree for USP25 (if available)
TreeFam Gene Tree for USP25 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for USP25 gene

USP28²

1 SIMAP similar gene for USP25 using alignment to 4 protein entries: UBP25_HUMAN (see all proteins):

USP28

USP25 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 21 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs188147083^1,2		--	17100555(+)	`AGAAAC/TGCTGA`	1	--	us2k¹		0	--	--	--	--
rs79967712^1,2		--	17100557(+)	`AAATGC/TTGAGT`	1	--	us2k¹		2		CSA WA	120
rs139117380^1,2	C,	--	17100597(+)	`AATGA-/ACAGTCT`	1	--	us2k¹		0	--	--	--	--
rs115654109^1,2	F,	--	17100807(+)	`AATTTT/GCTTGA`	1	--	us2k¹		1		WA	118
rs200950767^1,2		--	17100841(+)	`CTAAA-/AAAC AAACA`	1	--	us2k¹		0	--	--	--	--
rs149686201^1,2		--	17100897(+)	`GTTAGC/TATATA`	1	--	us2k¹		0	--	--	--	--
rs146700823^1,2		--	17100971(+)	`GACAGC/GTCACC`	1	--	us2k¹		0	--	--	--	--
rs181230330^1,2		--	17101058(+)	`CAACCC/GTTTCA`	1	--	us2k¹		0	--	--	--	--
rs9974570^1,2	C,F,H,	--	17101120(+)	`TGCAAT/GTTGTT`	1	--	us2k¹ tfbs³		8		NS NA WA	1088
rs115255826^1,2		--	17101132(+)	`AATATC/GAAATG`	1	--	us2k¹		1		WA	118

HapMap Linkage Disequilibrium report for USP25 (17102344 - 17252377 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for USP25: --

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing USP25

DNA2.0 Custom Variant and Variant Library Synthesis for USP25

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

USP25 for disorders           About GeneDecksing

OMIM gene information: 604736    OMIM disorders: --

9 diseases for USP25:    About MalaCards

non-small cell lung carcinoma panic disorder acne lung carcinoma

crohn's disease parkinson's disease lung cancer carcinoma

prostatitis

Human Genome Epidemiology (HuGE) Navigator: USP25 (2 documents)

Export disorders for USP25 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for USP25 gene, integrated from 9 sources (see all 35):
(articles sorted by number of sources associating them with USP25)

Utopia: connect your pdf to the dynamic
world of online information

Narrowing of the region of allelic loss in 21q11-21 in squamous non-small cell lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the deleted segment. (PubMed id 10612803)^{1, 2, 3} Groet J....Nizetic D. (2000)
Functional interaction between the ubiquitin-specific protease 25 and the SYK tyrosine kinase. (PubMed id 19909739)^{1, 2, 9} Cholay M....Daviet L. (2010)
Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. (PubMed id 18538659)^{1, 2, 9} Meulmeester E....Melchior F. (2008)
Characterization of alternatively spliced products and tissue- specific isoforms of USP28 and USP25. (PubMed id 11597335)^{1, 2, 9} Valero R.... Marfany G. (2001)
USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. (PubMed id 10644437)^{1, 2, 9} Valero R.... Gonzalez-Duarte R. (1999)
The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins. (PubMed id 16501887)^{1, 2, 9} Bosch-Comas A....Marfany G. (2006)
The UBA-UIM domains of the USP25 regulate the enzyme ubiquitination state and modulate substrate recognition. (PubMed id 19440361)^{1, 2} Denuc A....Marfany G. (2009)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
Human and mouse proteases: a comparative genomic approach. (PubMed id 12838346)^{1, 3} Puente X.S.... Lopez-Otin C. (2003)
A census of human soluble protein complexes. (PubMed id 22939629)¹ Havugimana P.C....Emili A. (2012)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 29761	HGNC: 12624	AceView: USP25	Ensembl:ENSG00000155313	euGenes: HUgn29761
ECgene: USP25	H-InvDB: USP25

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for USP25	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for USP25	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for USP25 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for USP25 gene:
Search GeneIP for patents involving USP25

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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USP25 Gene

ubiquitin specific peptidase 25

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USP25 Gene

ubiquitin specific peptidase 25

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