Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



USP14 Gene

protein-coding   GIFtS: 64
GCID: GC18P000148

Ubiquitin Specific Peptidase 14 (TRNA-Guanine Transglycosylase)

(Previous name: ubiquitin specific protease 14 (tRNA-guanine transglycosylase))
  See USP14-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Ubiquitin Specific Peptidase 14 (TRNA-Guanine Transglycosylase)1 2     TRNA-Guanine Transglycosylase, 60-KD Subunit2
TGT2 3 5     Ubiquitin Carboxyl-Terminal Hydrolase 142
Ubiquitin Specific Protease 14 (TRNA-Guanine Transglycosylase)1 2     Ubiquitin Thiolesterase 142
Deubiquitinating Enzyme 142 3     Ubiquitin-Specific Processing Protease 142
Ubiquitin Thioesterase 142 3     EC 3.4.19.123
Ubiquitin-Specific-Processing Protease 142 3     EC 3.1.2.158

External Ids:    HGNC: 126121   Entrez Gene: 90972   Ensembl: ENSG000001015577   OMIM: 6072745   UniProtKB: P545783   

Export aliases for USP14 gene to outside databases

Previous GC identifers: GC18P000442 GC18P000146


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for USP14 Gene:
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a
deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the
ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results
in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3
weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice
variants, encoding different isoforms, have been characterized. (provided by RefSeq, Jul 2008)

GeneCards Summary for USP14 Gene:
USP14 (ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)) is a protein-coding gene. Diseases associated with USP14 include ovarian serous cystadenocarcinoma. GO annotations related to this gene include ubiquitin-specific protease activity and ubiquitin thiolesterase activity.

UniProtKB/Swiss-Prot: UBP14_HUMAN, P54578
Function: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated
proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the
proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation
of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a
physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition
by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable
for synaptic development and function at neuromuscular junctions (NMJs)

summary for USP14 Gene:
Deubiquitinating enzymes (DUBs) are a group of proteases that catalyze the cleavage of protein-ubiquitin
bonds. By removing these modifications, DUBs counteract the activity of ubiquitin ligases, which add
ubiquitin molecules to target proteins.

Gene Wiki entry for USP14 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000018.9  NC_018929.2  NT_010859.15  
Regulatory elements:
   Regulatory transcription factor binding sites in the USP14 gene promoter:
         AP-1   RREB-1   FOXD1   CUTL1   MIF-1   LCR-F1   NF-E2 p45   HNF-1A   HNF-1   NF-E2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidUSP14 promoter sequence
   Search Chromatin IP Primers for USP14

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat USP14


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 18p11.32   Ensembl cytogenetic band:  18p11.32   HGNC cytogenetic band: 18p11.32

USP14 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
USP14 gene location

GeneLoc information about chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18P000148:  view genomic region     (about GC identifiers)

Start:
158,383 bp from pter      End:
214,629 bp from pter
Size:
56,247 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: UBP14_HUMAN, P54578 (See protein sequence)
Recommended Name: Ubiquitin carboxyl-terminal hydrolase 14  
Size: 494 amino acids; 56069 Da
Subunit: Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1
Caution: Was originally (Ref.1) thought to be a guanine tRNA-ribosyltransferase
Sequence caution: Sequence=CR976282; Type=Frameshift; Positions=288;
2 PDB 3D structures from and Proteopedia for USP14:
2AYN (3D)        2AYO (3D)    
Secondary accessions: J3QRZ5 Q53XY5
Alternative splicing: 2 isoforms:  P54578-1   P54578-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for USP14: NX_P54578

Explore proteomics data for USP14 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys49, Lys62, Lys130, Lys214, Lys291, Lys300, Lys348, Lys441, Lys443, Lys449
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for USP14 (P54578) (see all 5)
     SGGGDWH  NLGNTCYMN  PPMVFKAQLFALT  QMMRVLQQKLEAIEDD 


    See USP14 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001032411.1  NP_005142.1  

    ENSEMBL proteins: 
     ENSP00000464447   ENSP00000383125   ENSP00000463891   ENSP00000373083   ENSP00000261601  
     ENSP00000462315   ENSP00000473563  

    USP14 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for USP14
    Enzo Life Sciences proteins for USP14
    OriGene Purified Proteins for USP14
    OriGene Protein Over-expression Lysate for USP14
    OriGene MassSpec for USP14
    OriGene Custom Protein Services for USP14
    GenScript Custom Purified and Recombinant Proteins Services for USP14
    Novus Biologicals USP14 Proteins
    Novus Biologicals USP14 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for USP14
    Cloud-Clone Corp. Proteins for USP14

     
    Search eBioscience for Proteins for USP14 

     
    antibodies-online proteins for USP14 (12 products) 

     
    antibodies-online peptides for USP14

    USP14 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for USP14 
    OriGene Antibodies for USP14
    OriGene Custom Antibody Services for USP14
    Novus Biologicals USP14 Antibodies
    Abcam antibodies for USP14
    Cloud-Clone Corp. Antibodies for USP14
    ThermoFisher Antibody for USP14
    LSBio Antibodies in human, mouse, rat for USP14
    antibodies-online antibodies for USP14 (43 products) 

    USP14 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for USP14
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for USP14
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for USP14
    Cloud-Clone Corp. CLIAs for USP14
    Search eBioscience for ELISAs for USP14 
    antibodies-online kits for USP14 (10 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    USP: Ubiquitin-specific peptidases

    5 InterPro protein domains:
     IPR001394 Peptidase_C19_UCH
     IPR018200 Pept_C19ubi-hydrolase_C_CS
     IPR019954 Ubiquitin_CS
     IPR000626 Ubiquitin_dom
     IPR028889 UCH/PAN2

    Graphical View of Domain Structure for InterPro Entry P54578

    ProtoNet protein and cluster: P54578

    2 Blocks protein domains:
    IPB000626 Ubiquitin domain
    IPB001394 Ubiquitin thiolesterase


    UniProtKB/Swiss-Prot: UBP14_HUMAN, P54578
    Similarity: Belongs to the peptidase C19 family. USP14/UBP6 subfamily
    Similarity: Contains 1 ubiquitin-like domain
    Similarity: Contains 1 USP domain


    Find genes that share domains with USP14           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: UBP14_HUMAN, P54578
    Function: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated
    proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the
    proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation
    of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a
    physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition
    by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable
    for synaptic development and function at neuromuscular junctions (NMJs)
    Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by
    the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)

         Genatlas biochemistry entry for USP14:
    ubiquitin specific protease 14,deubiquitin enzyme family

         Enzyme Numbers (IUBMB): EC 3.1.2.152 EC 3.4.19.121

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004197cysteine-type endopeptidase activity TAS9827704
    GO:0004221ubiquitin thiolesterase activity IDA19106094
    GO:0004843ubiquitin-specific protease activity IDA--
    GO:0004866endopeptidase inhibitor activity IMP18162577
    GO:0005515protein binding IPI19106094
         
    Find genes that share ontologies with USP14           About GenesLikeMe


    Phenotypes:
         10 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Usp14):
     behavior/neurological  cellular  growth/size/body  homeostasis/metabolism  integument 
     mortality/aging  muscle  nervous system  reproductive system  skeleton 

    Find genes that share phenotypes with USP14           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for USP14
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for USP14

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for USP14
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for USP14

    miRNA
    Products:
        
    miRTarBase miRNAs that target USP14:
    hsa-mir-215-5p (MIRT024696), hsa-mir-192-5p (MIRT026381), hsa-mir-26b-5p (MIRT029168), hsa-let-7b-5p (MIRT052341), hsa-let-7a-5p (MIRT052527), hsa-mir-204-5p (MIRT047022)

    Block miRNA regulation of human, mouse, rat USP14 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate USP14 (see all 53):
    hsa-miR-26a-2* hsa-miR-15a hsa-miR-200a hsa-miR-219-5p hsa-miR-488 hsa-miR-508-3p hsa-miR-141 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidUSP14 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for USP14
    Predesigned siRNA for gene silencing in human, mouse, rat USP14

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for USP14

    Clone
    Products:
         
    OriGene clones in human, mouse for USP14 (see all 15)
    OriGene ORF clones in mouse, rat for USP14
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): USP14 (NM_001037334)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for USP14
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP14
    Addgene plasmids for USP14 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for USP14
    Browse ESI BIO Cell Lines and PureStem Progenitors for USP14 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP14


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    UBP14_HUMAN, P54578: Cytoplasm. Cell membrane; Peripheral membrane protein
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    cytosol3
    nucleus2
    endoplasmic reticulum1
    extracellular1
    golgi apparatus1

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000502proteasome complex IEA--
    GO:0005737cytoplasm IDA--
    GO:0005886plasma membrane IEA--
    GO:0009986cell surface IDA19106094
    GO:0016023cytoplasmic membrane-bounded vesicle IDA19106094

    Find genes that share ontologies with USP14           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for USP14 About    
    See pathways by source

    SuperPathContained pathways About
    1Ubiquitin-Proteasome Dependent Proteolysis
    Ubiquitin-Proteasome Dependent Proteolysis
    2Protein Stability
    Protein Stability
    3Neuroscience
    Neuroscience


    Find genes that share SuperPaths with USP14           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for USP14
        Ubiquitin-Proteasome Dependent Proteolysis

    2 Cell Signaling Technology (CST) Pathways for USP14
        Neuroscience
    Protein Stability


        Pathway & Disease-focused RT2 Profiler PCR Array including USP14: 
              Unfolded Protein Response in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for USP14

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for USP14 (P545781, 2, 3 ENSP000002616014) via UniProtKB, MINT, STRING, and/or I2D (see all 336)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006511ubiquitin-dependent protein catabolic process IEA--
    GO:0007268synaptic transmission IEA--
    GO:0010951negative regulation of endopeptidase activity IMP18162577
    GO:0016579protein deubiquitination IDA--
    GO:0050920regulation of chemotaxis IMP19106094

    Find genes that share ontologies with USP14           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    9 ApexBio Compounds for USP14     About this table
    CompoundAction CAS #
    IU1potent and selective small-molecule inhibitor of Usp14[314245-33-5]
    LDN 57444Inhibitor of ubiquitin C-terminal hydrolase-L1 (UCH-L1) activity[668467-91-2]
    NSC 632839 hydrochlorideInhibitor of ubiquitin isopeptidase activity[157654-67-6]
    P 22077Inhibitor of ubiquitin-specific protease (USP) 7[1247819-59-5]
    P005091Ubiquitin-specific protease 7 (USP7) inhibitor[882257-11-6]
    PR-619Cell-permeable, non selective, reversible deubiquitinases/deubiquitylases (DUBs) inhibitor[2645-32-1]
    SJB2-043novel and potent USP1 (ubiquitin-specific protease 1) inhibitor. [63388-44-3]
    Vialinin AInhibitor of USP4, USP5/isopeptidase T (IsoT) and UCH-L1 DUB activity[858134-23-3]
    WP1130Cell permeable deubiquitinase (DUB) inhibitor[856243-80-6]

    Compounds for USP14 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    P 22077USP7 inhibitor[1247819-59-5]
    HBX 41108Selective USP7 inhibitor[924296-39-9]
    IU1USP14 inhibitor[314245-33-5]
    LDN 57444Ubiquitin C-terminal hydrolase-L1 (UCH-L1) inhibitor[668467-91-2]
    NSC 632839 hydrochlorideInhibitor of ubiquitin isopeptidase activity[157654-67-6]

    1 HMDB Compound for USP14    About this table
    CompoundSynonyms CAS #PubMed Ids
    WaterDihydrogen oxide (see all 2)7732-18-5--

    2 Novoseek inferred chemical compound relationships for USP14 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    queuine 92.8 4 20354154 (2), 12877882 (1)
    trna 54.5 4 12877882 (2), 17673081 (1)



    Find genes that share compounds with USP14           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for USP14 gene (2 alternative transcripts): 
    NM_001037334.1  NM_005151.3  

    Unigene Cluster for USP14:

    Ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
    Hs.464416  [show with all ESTs]
    Unigene Representative Sequence: NM_005151
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000582707 ENST00000400266 ENST00000580410 ENST00000578942 ENST00000383589
    ENST00000261601(uc002kkf.1 uc002kkg.1 uc010wyr.1) ENST00000581983
    ENST00000583119 ENST00000578786
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat USP14 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate USP14 (see all 53):
    hsa-miR-26a-2* hsa-miR-15a hsa-miR-200a hsa-miR-219-5p hsa-miR-488 hsa-miR-508-3p hsa-miR-141 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidUSP14 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for USP14
    Predesigned siRNA for gene silencing in human, mouse, rat USP14
    Clone
    Products:
         
    OriGene clones in human, mouse for USP14 (see all 15)
    OriGene ORF clones in mouse, rat for USP14
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): USP14 (NM_001037334)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for USP14
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP14
    Addgene plasmids for USP14 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for USP14
    OriGene qSTAR qPCR primer pairs in human, mouse for USP14
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat USP14
      QuantiTect SYBR Green Assays in human, mouse, rat USP14
      QuantiFast Probe-based Assays in human, mouse, rat USP14

    Additional mRNA sequence: 

    AK098497.1 AK297605.1 AK315438.1 BC003556.1 BT007183.1 U30888.1 

    23 DOTS entries:

    DT.100817195  DT.91707912  DT.455834  DT.100817205  DT.100639435  DT.100817207  DT.95309692  DT.92448768 
    DT.95133414  DT.95133370  DT.92447230  DT.92448763  DT.92448767  DT.121091275  DT.40305664  DT.95139503 
    DT.100032014  DT.100817197  DT.101965453  DT.92447215  DT.92448759  DT.100786553  DT.403432 

    Selected AceView cDNA sequences (see all 403):

    AK095516 BF000293 N59188 BG113320 AL135288 CA313816 AI765007 AL546236 
    AI475012 AI536829 NM_005151 AW631048 BX459969 AA235178 CR604259 AI818355 
    BT007183 T41055 BU616778 CA419115 AA243532 BM987237 BX490182 BQ020143 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for USP14 (see all 7)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19a · 19b
    SP1:                                                        -     -                                                                                 
    SP2:                                      -                 -     -                                                                                 
    SP3:                                                                                                                          -                     
    SP4:                                                                                                                                                
    SP5:                                                                                                                                                


    ECgene alternative splicing isoforms for USP14

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    USP14 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    USP14 Expression
    About this image


    USP14 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Liver (Hepatobiliary System)
             Perivenous Hepatocytes Liver Lobule
     
     Bone (Muscoskeletal System)
             Mandibular Condyle
     
     Brain (Nervous System)
             Medulla Oblongata
    USP14 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    USP14 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.464416
        Pathway & Disease-focused RT2 Profiler PCR Array including USP14: 
              Unfolded Protein Response in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for USP14
    OriGene qSTAR qPCR primer pairs in human, mouse for USP14
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat USP14
    QuantiTect SYBR Green Assays in human, mouse, rat USP14
    QuantiFast Probe-based Assays in human, mouse, rat USP14
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP14

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for USP14 gene from Selected species (see all 27)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Usp141 , 5 ubiquitin specific peptidase 141, 5 91.48(n)1
    96.96(a)1
      18 (4.91 cM)5
    590251  NM_021522.41  NP_067497.21 
     99954325 
    chicken
    (Gallus gallus)
    Aves USP141 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) 82.66(n)
    90.45(a)
      421063  XM_004939755.1  XP_004939812.1 
    lizard
    (Anolis carolinensis)
    Reptilia USP146
    ubiquitin specific peptidase 14 (tRNA-guanine tran...
    90(a)
    1 ↔ 1
    4(39289789-39316560)
    African clawed frog
    (Xenopus laevis)
    Amphibia BU914627.12   -- 82.38(n)    BU914627.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufk63d092 wufk63d09 77.75(n)   335736  BC044553.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG53841 , 3 protein deubiquitination
    ubiquitin-specific protease3
    CG53841
    55(a)3
    57.51(n)1
    56.87(a)1
      343871  NM_001273392.11  NP_001260321.11 
    worm
    (Caenorhabditis elegans)
    Secernentea usp-141 usp-14 51.88(n)
    47.07(a)
      175105  NM_064605.8  NP_497006.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes UBP6(YFR010W)4
    UBP61
    Ubiquitin-specific protease situated in the base subcomplex more4
    UBP61
    47.78(n)1
    37.39(a)1
      6(165067-166566)4
    8505621, 4  NP_116665.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons UBP61 UBP6 53.38(n)
    46.55(a)
      841596  NM_104049.3  NP_564596.1 
    rice
    (Oryza sativa)
    Liliopsida Os.90892 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 76.4(n)    AK066233.1 


    ENSEMBL Gene Tree for USP14 (if available)
    TreeFam Gene Tree for USP14 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for USP14 (see all 1212)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 18 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1176388891,2
    C,F--156619(+) GTCCTT/CATAAC 2 -- us2k11Minor allele frequency- C:0.02NA 120
    rs5572491,2
    C,F,A,H--156739(-) ggaaaC/Acaaaa 2 -- us2k18Minor allele frequency- A:0.44NA WA CSA EA 368
    rs118735921,2
    C,F,H--156771(+) ttctaC/Ttatac 2 -- us2k13Minor allele frequency- T:0.17NA WA 6
    rs2004018641,2
    C--156810(+) GCAGTA/GGCTTA 2 -- us2k10--------
    rs1842055621,2
    --156876(+) GAGTTA/CAACAC 2 -- us2k10--------
    rs1870365231,2
    --156916(+) TTTACA/GAAACA 2 -- us2k10--------
    rs1127072291,2
    C,F--156976(+) TGAGAG/AGATAG 2 -- us2k11Minor allele frequency- A:0.03NA 120
    rs5621171,2
    C,F,H--157065(-) tgtttT/Ggagac 2 -- us2k1 tfbs35Minor allele frequency- G:0.13NA CSA WA 244
    rs1928628141,2
    --157256(+) GCCACA/GTCGAT 2 -- us2k10--------
    rs65059301,2
    C,F,A,H--157275(+) cctttC/Tatgaa 2 -- us2k1 trp311Minor allele frequency- T:0.31NA WA CSA EA 376

    HapMap Linkage Disequilibrium report for USP14 (158383 - 214629 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for USP14:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2675103CNV Deletion23128226
    esv2658900CNV Deletion23128226
    nsv512512CNV Loss21212237
    nsv909285CNV Gain21882294
    nsv518642CNV Gain19592680

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing USP14
    DNA2.0 Custom Variant and Variant Library Synthesis for USP14

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 607274    OMIM disorders: --

    1 disease for USP14:    
    About MalaCards
    ovarian serous cystadenocarcinoma

    1 disease from the University of Copenhagen DISEASES database for USP14:
    Ovarian serous cystadenocarcinoma

    Find genes that share disorders with USP14           About GenesLikeMe

    Human Genome Epidemiology (HuGE) Navigator: USP14 (1 document)

    Export disorders for USP14 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for USP14 gene, integrated from 10 sources (see all 65):
    (articles sorted by number of sources associating them with USP14)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Deubiquitination of CXCR4 by USP14 is critical for both CXCL12- induced CXCR4 degradation and chemotaxis but not ERK activation. (PubMed id 19106094)1, 2 Mines M.A.... Fan G.H. (J. Biol. Chem. 2009)
    2. USP14 inhibits ER-associated degradation via interaction with IRE1alpha. (PubMed id 19135427)1, 2 Nagai A.... Ichijo H. (Biochem. Biophys. Res. Commun. 2009)
    3. Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome. (PubMed id 18162577)1, 2 Koulich E.... DeMartino G.N. (Mol. Biol. Cell 2008)
    4. Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14. (PubMed id 16211010)1, 2 Hu M.... Shi Y. (EMBO J. 2005)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    6. Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport. (PubMed id 14499622)1, 2 Reuter T.Y....Huber P.A. (Exp. Cell Res. 2003)
    7. Human and mouse proteases: a comparative genomic approach. (PubMed id 12838346)1, 3 Puente X.S.... Lopez-Otin C. (Nat. Rev. Genet. 2003)
    8. Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases. (PubMed id 16465409)1, 9 Shinji S....Tajiri T. (Oncol. Rep. 2006)
    9. Overexpression of USP14 protease reduces I-I_B protein levels and increases cytokine release in lung epithelial cells. (PubMed id 23615914)1 Mialki R.K....Zhao Y. (J. Biol. Chem. 2013)
    10. Conformational dynamics control ubiquitin-deubiquitinase interactions and influence in vivo signaling. (PubMed id 23801757)1 Phillips A.H....Corn J.E. (Proc. Natl. Acad. Sci. U.S.A. 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 9097 HGNC: 12612 AceView: USP14 Ensembl:ENSG00000101557 euGenes: HUgn9097
    ECgene: USP14 H-InvDB: USP14

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for USP14 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for USP14 gene:
    Search GeneIP for patents involving USP14

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Recombinant/Natural Proteins for USP14 (USP14)   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for USP14   OriGene RNAi products in human, mouse, rat for USP14  
     OriGene qPCR primer pairs and template standards for USP14   OriGene Protein Over-expression Lysate for USP14  
     OriGene MassSpec for USP14   OriGene clones in human, mouse for USP14  
     OriGene qSTAR qPCR primer pairs in human, mouse for USP14   OriGene Purified Protein for USP14  
     OriGene ORF clones in mouse, rat for USP14   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for USP14   OriGene Custom Protein Services for USP14  

     
     
     Block miRNA regulation of human, mouse, rat USP14 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing USP14
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat USP14 Predesigned siRNA for gene silencing in human, mouse, rat USP14
     QuantiFast Probe-based Assays in human, mouse, rat USP14 QuantiTect SYBR Green Assays in human, mouse, rat USP14
     PCR Arrays including human, mouse, rat USP14 Search Chromatin IP Primers for USP14
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat USP14  Search GeneGlobe Interaction Network for USP14
     Regulatory tfbs in USP14 promoter
     GenScript Custom Purified and Recombinant Proteins Services for USP14 GenScript cDNA clones with any tag delivered in your preferred vector for USP14
     GenScript Custom Assay Services for USP14 GenScript Custom overexpressing Cell Line Services for USP14
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for USP14 

     Tocris compounds for USP14
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for USP14
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     USP14 antibodies
     USP14 proteins
     USP14 lysates
     Antibodies for USP14
     See all of Abcam's Antibodies, Kits and Proteins for USP14
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for USP14
     Proteins for USP14
     Antibodies for USP14
     ELISAs for USP14
     CLIAs for USP14



     Browse ESI BIO Cell Lines and PureStem Progenitors for USP14
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for USP14
     SwitchGear 3'UTR luciferase reporter plasmids for USP14
     SwitchGear Promoter luciferase reporter plasmids for USP14
     ThermoFisher Antibody for USP14
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat USP14
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for USP14
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for USP14
     LSBio Antibodies in human, mouse, rat for USP14
     ApexBio compounds for USP14
     Addgene plasmids for USP14
      Search eBioscience for proteins for USP14
      Search eBioscience for elisas for USP14
      eBioscience FlowRNA Probe Sets
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     antibodies-online antibodies for USP14 (43 products)
     antibodies-online kits for USP14 (10 products)
     antibodies-online peptides for USP14
     antibodies-online proteins for USP14 (12 products)
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      USP14 gene at Home site.
    Version: 3.12.234 23 Oct 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4