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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

UGT2B28 Gene

protein-coding   GIFtS: 53
GCID: GC04P070194

UDP glucuronosyltransferase 2 family, polypeptide B28

(Previous names: UDP glycosyltransferase 2 family, polypeptide B28 )
 Explore 5 diseases affiliated with
UGT2B28 via our new
 Human Malady Compendium 
Biological research products
for UGT2B28
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
UDP Glucuronosyltransferase 2 Family, Polypeptide B281 2
UDP Glycosyltransferase 2 Family, Polypeptide B281 2
UDPGT 2B282 3
EC 2.4.1.173 8
UDP-Glucuronosyltransferase 2B282

External Ids:    HGNC: 134791   Entrez Gene: 544902   Ensembl: ENSG000001352267   OMIM: 6064975   UniProtKB: Q9BY643   

Export aliases for UGT2B28 gene to outside databases

Previous GC identifers: GC04M070372 GC04P070396 GC04P070461 GC04P070327 GC04P065944


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for UGT2B28:
This gene encodes a member of the uridine diphosphoglucuronosyltransferase protein family. The encoded enzyme catalyzes
the transfer of glucuronic acid from uridine diphosphoglucuronic acid to a diverse array of substrates including
steroid hormones and lipid-soluble drugs. This process, known as glucuronidation, is an intermediate step in the
metabolism of steroids. Two transcript variants encoding different isoforms have been found for this gene. While both
isoforms are targeted to the endoplasmic reticulum, only the longer isoform appears to be active. (provided by RefSeq,
May 2011)

UniProtKB/Swiss-Prot: UDB28_HUMAN, Q9BY64
Function: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics
and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane
3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NT_022778.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the UGT2B28 gene promoter:
         C/EBPbeta   AML1a   Nkx2-2   FOXD1   E47   FOXO1a   Hand1   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidUGT2B28 promoter sequence
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Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat UGT2B28


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q13.2   Ensembl cytogenetic band:  4q13.2   HGNC cytogenetic band: 4q13.3

UGT2B28 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
UGT2B28 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P070194:  view genomic region     (about GC identifiers)

Start:
70,146,217 bp from pter      End:
70,160,768 bp from pter
Size:
14,552 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: UDB28_HUMAN, Q9BY64 (See protein sequence)
Recommended Name: UDP-glucuronosyltransferase 2B28 precursor  
Size: 529 amino acids; 60906 Da
Subcellular location: Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass
membrane protein
Sequence caution: Sequence=AAK31809.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing;
Secondary accessions: B5BUM0 Q9BY62 Q9BY63
Alternative splicing: 2 isoforms:  Q9BY64-1   Q9BY64-2   

Explore the universe of human proteins at neXtProt for UGT2B28: NX_Q9BY64

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BY64

  • 4/26 DME Specific Peptides for UGT2B28 (Q9BY64) (see all 26)
     DLLNALK  AFITHGG  MTFMERV  CGKVLVW 

    UGT2B28 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001193933.1  NP_444267.1  

    ENSEMBL proteins: 
     ENSP00000334276   ENSP00000427399  
    Reactome Protein details: Q9BY64
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    Uscn Proteins for UGT2B28

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005783endoplasmic reticulum IDA11300766
    GO:0005789endoplasmic reticulum membrane IEA--
    GO:0005792microsome ----
    GO:0016021integral to membrane IEA--


    UGT2B28 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    UGT2B28 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR002213 UDP_glucos_trans

    Graphical View of Domain Structure for InterPro Entry Q9BY64

    ProtoNet protein and cluster: Q9BY64

    1 Blocks protein family: IPB002213 UDP-glucoronosyl/UDP-glucosyl transferase

    UniProtKB/Swiss-Prot: UDB28_HUMAN, Q9BY64
    Similarity: Belongs to the UDP-glycosyltransferase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: UDB28_HUMAN, Q9BY64
    Function: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics
    and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane
    3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active
    Catalytic activity: UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside

    Enzyme Number (IUBMB): EC 2.4.1.171 2

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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0015020glucuronosyltransferase activity IEA--


    UGT2B28 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/7 super-pathways (see all 7About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Glucuronidation
    Glucuronidation1.00
    Porphyrin and chlorophyll metabolism0.38
    Glucuronidation0.67
    Other types of O-glycan biosynthesis0.36
    Starch and sucrose metabolism0.42
    Drug metabolism - other enzymes0.32
    2Drug metabolism - cytochrome P450
    Drug metabolism - cytochrome P4501.00
    Retinol metabolism0.40
    Metabolism of xenobiotics by cytochrome P4500.73
    3Biological oxidations
    Biological oxidations1.00
    metapathway biotransformation0.41
    Phase II conjugation0.52
    4Metabolism
    Metabolism1.00
    Metabolic pathways0.38
    5Pentose and glucuronate interconversions
    Pentose and glucuronate interconversions1.00
    Ascorbate and aldarate metabolism0.62

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for UGT2B28
        Estradiol metabolism


    1 GeneGo (Thomson Reuters) Pathway for UGT2B28
        Estradiol metabolism

    2 BioSystems Pathways for UGT2B28 
        Glucuronidation
    metapathway biotransformation

    4        Reactome Pathways for UGT2B28
        Metabolism
    Biological oxidations
    Phase II conjugation
    Glucuronidation


    5/11         Kegg Pathways  (Kegg details for UGT2B28) (see all 11):
        Pentose and glucuronate interconversions
    Ascorbate and aldarate metabolism
    Steroid hormone biosynthesis
    Starch and sucrose metabolism
    Other types of O-glycan biosynthesis


    UGT2B28 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for UGT2B28

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006805xenobiotic metabolic process IDA11300766
    GO:0008152metabolic process IDA11300766


    UGT2B28 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for UGT2B28

    10/85 HMDB Compounds for UGT2B28 (see all 85)    About this table
    CompoundSynonyms CAS #PubMed Ids
    (23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide)(23S)-23,25-dihydroxy-24-oxovitamin D3 23-(beta-glucuronide) (see all 5)----
    1-(alpha-Methyl-4-(2-methylpropyl)benzeneacetate)-beta-D-Glucopyranuronic acidIbuprofen acyl glucuronide;(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-[2-[4-(2-methylpropyl)phenyl]propanoyloxy]oxane-2-carboxylic acid (see all 5)115075-59-7--
    1-Salicylate glucuronide1-salicylic acid glucuronide ----
    11-Hydroxyprogesterone 11-glucuronide11-hydroxyprogesterone 11-glucuronoside (see all 5)77710-64-6--
    11-Oxo-androsterone glucuronide11-oxo-etiocholanolone glucuronide (see all 3)----
    11-beta-hydroxyandrosterone-3-glucuronide11-beta-hydroxyetiocholanolone-3-glucuronide ----
    15-hydroxynorandrostene-3,17-dione glucuronide15-HNADG (see all 5)131749-23-0--
    16-alpha,17-beta-estriol 17-beta-D-glucuronideestra-1,3,5(10)-triene-3,16alpha,17beta-triol 17-D-glucuronide (see all 7)7219-89-8--
    17-alpha-estradiol-3-glucuronide ----
    17-beta-estradiol glucuronide ----
    Search CenterWatch for drugs/clinical trials and news about UGT2B28 / UDB28 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    About This Section

    REFSEQ mRNAs for UGT2B28 gene (2 alternative transcripts): 
    NM_001207004.1  NM_053039.1  

    Unigene Cluster for UGT2B28:

    UDP glucuronosyltransferase 2 family, polypeptide B28
    Hs.653154  [show with all ESTs]
    Unigene Representative Sequence: AF177272
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000335568(uc003hej.3) ENST00000511240(uc010ihr.3)

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    Additional cDNA sequence: 

    AB451456.1 AF177272.1 AF177273.1 

    3 DOTS entries:

    DT.121219029  DT.91760196  DT.95372476 

    24/35 AceView cDNA sequences (see all 35):

    CB164598 NM_001075 T57898 T46839 H69250 CD013994 CD013995 CB161428 
    BG189133 BV199400 BV199399 AV696157 BV199398 BG201991 AI688814 BV201798 
    BV172697 CB113377 BG212458 CD610478 AI032906 T57980 R00510 N78136 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    UGT2B28 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATCAACCAGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See UGT2B28 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for UGT2B28

    SOURCE GeneReport for Unigene cluster: Hs.653154

    UniProtKB/Swiss-Prot: UDB28_HUMAN, Q9BY64
    Tissue specificity: Expressed in the liver, breast and kidney

        SABiosciences Expression via Pathway-Focused PCR Array including UGT2B28: 
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    In Situ
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    Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for UGT2B28

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for UGT2B28 gene from 3/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves F1NMB3_CHICK6
    Uncharacterized protein
    56(a)
    1 → many
    4(53288491-53295396)
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    --
    54(a)
    54(a)
    many ↔ many
    many ↔ many
    6(58629882-58649612)
    6(58564243-58578541)
    zebrafish
    (Danio rerio)
    Actinopterygii BX571679.16
    ugt2a46
    (see all 15)
    --
    UDP glucuronosyltransferase 2 family, polypeptide ...
    (see all 15)
    61(a)
    51(a)
    (see all 15)
    many ↔ many
    many ↔ many
    (see all 15)
    5(9986135-9988976)
    5(72956618-72977951)


    ENSEMBL Gene Tree for UGT2B28 (if available)
    TreeFam Gene Tree for UGT2B28 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for UGT2B28 gene
    UGT2A22  UGT1A82  UGT1A72  UGT3A12  UGT2B72  UGT2B112  UGT1A32  UGT1A12  
    UGT1A52  UGT3A22  UGT2B102  UGT1A102  UGT1A62  UGT82  UGT2B152  UGT2B42  
    UGT2B172  UGT2A32  UGT1A92  UGT1A42  
    18/26 SIMAP similar genes for UGT2B28 using alignment to 1 protein entry:     UDB28_HUMAN(see all similar genes):
    UGT2B11    UGT2B7    UGT2B4    UGT2B17    UGT2B15    UGT2B10
    UGT2A3    UGT1A9S    UGT2A1    UGT1A5    UGT1A8S    BILIQTL1
    UGT1A6    UGT1A8    UGT1A1    UGT1A4    UGT1A7    UGT1A10

    UGT2B28 for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for UGT2B28
    PGOHUM00000250778 PGOHUM00000259942


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 18 variations for UGT2B28
         15/17 CNVs (see all 17): 68742 2528 1394 92185 1674 38069 32616 68743 38967 7449 0701 80355 3499 7450 2073
         1 Inversion: 29481
    Human Gene Mutation Database (HGMD): UGT2B28
    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    UGT2B28 for disorders           About GeneDecksing

    OMIM gene information: 606497    OMIM disorders: --

    5 diseases for UGT2B28:    About MalaCards
    kernicterus    hearing loss    prostate cancer    cholesterol
    prostatitis

    Human Genome Epidemiology (HuGE) Navigator: UGT2B28 (2 documents)

    Export disorders for UGT2B28 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for UGT2B28 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with UGT2B28)
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    1. Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase. (PubMed id 11300766)1, 2, 3, 9 Levesque E....Belanger A. (2001)
    2. Quantitative analysis of UGT2B28 mRNA expression by r eal-time RT-PCR and application to human tissue distribution study. (PubMed id 21679149)1 Ohno S. and Nakajin S. (2011)
    3. Genetic variation of genes involved in dihydrotestost erone metabolism and the risk of prostate cancer. (PubMed id 20056642)1 Setlur S.R....Demichelis F. (2010)
    4. Copy-number variations (CNVs) of the human sex steroi d metabolizing genes UGT2B17 and UGT2B28 and their associations with a UGT2B15 functional polymorphism. (PubMed id 19572376)1 MAcnard V....LAcvesque E. (2009)
    5. Genetic variants in TPMT and COMT are associated with hearing loss in children receiving cisplatin chemotherapy. (PubMed id 19898482)1 Ross C.J....Hayden M.R. (2009)
    6. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. (PubMed id 19054851)2 Goshima N.... Nomura N. (2008)
    7. Differential localisation of UDP-glucuronosyltransferase in kidney during human embryonic and fetal development. (PubMed id 7755484)9 Hume R....Burchell B. (1995)
    8. In vitro studies in microsomes from rat and human liver, kidney, and intestine suggest that perfluorooctanoic acid is not a substrate for microsomal UDP-glucuronosyltransferases. (PubMed id 16051554)9 Kemper R.A. and Nabb D.L. (2005)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54490 HGNC: 13479 AceView: UGT2B10 Ensembl:ENSG00000135226 euGenes: HUgn54490
    ECgene: UGT2B28 Kegg: 54490 H-InvDB: UGT2B28

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for UGT2B28 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    Patent Information for UGT2B28 gene:
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