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UBE2Z Gene

protein-coding   GIFtS: 54

GC17P044341
ubiquitin-conjugating enzyme E2Z
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 6.3.2.19 3
FLJ13855 1, 2
HOYS7 2
USE1 1, 2
Use1 3
Descriptions
UBA6-specific enzyme E2 2
Uba6-specific E2 conjugating enzyme 1 3
Ubiquitin carrier protein Z 3
Ubiquitin-protein ligase Z 3
ubiquitin-conjugating enzyme E2Z 2
External Ids
HGNC: 258471
Entrez Gene: 652642
UniProtKB: Q9H8323
Ensembl: ENSG000001592027
Search outside databases for aliases for UBE2Z gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for UBE2Z:
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many
signaling networks and requires a ubiquitin activating enzyme (E1), a ubiquitin conjugating enzyme
(E2), and a ubiquitin protein ligase (E3). UBE2Z is an E2 enzyme (Jin et al., 2007 [PubMed
17597759]).[supplied by OMIM]

UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
Specific substrate for UBE1L2, not charged with ubiquitin by UBE1. May be involved in apoptosis
regulation

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2Z gene  

Entrez Gene cytogenetic band: 17q21.32   Ensembl cytogenetic band:  17q21.32   HGNC cytogenetic band: 17q21.32

UBE2Z Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P044341:     (about GC identifiers)

Start:
44,340,730 bp from pter
End:
44,361,421 bp from pter
Size:
20,692 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010783.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 Z  
Size: 354 amino acids; 38210 Da
Subcellular location: Cytoplasm. Nucleus
Secondary accessions: A6N8M6 Q7L354 Q8TCM4 Q9H893
Alternative splicing: 2 isoforms:  Q9H832-1   Q9H832-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_075567.2  

    ENSEMBL proteins: 
    ENSP00000354201 ENSP00000386109 ENSP00000374259 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (UBE2Z)
    Human Recombinant Proteins from Abnova (UBE2Z)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005730NOT nucleolus IDA18029348
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for UBE2Z: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for UBE2Z
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (UBE2Z), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (UBE2Z)
    Novus Biologicals Antibodies for UBE2Z

    Assays for UBE2Z: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugat/RWD-like


       GeneDecks  UBE2Z for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H832

    ProtoNet protein and cluster: Q9H832

    1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832
    Similarity: Belongs to the ubiquitin-conjugating enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2Z)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2Z)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_023079

                  Applied Biosystems Silencer® siRNAs for UBE2Z

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2Z  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2Z  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBE2Z
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_023079
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_023079
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_023079 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_023079

    UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832
    Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
    Specific substrate for UBE1L2, not charged with ubiquitin by UBE1. May be involved in apoptosis
    regulation
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
    N-ubiquityllysine
    Enzyme Number (IUBMB): EC 6.3.2.19 

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842 ubiquitin-protein ligase activity IEA--
    GO:0016874 ligase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for UBE2Z):
     hsa04120 Ubiquitin mediated proteolysis

       GeneDecks  UBE2Z for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832
    Pathway: Protein modification; protein ubiquitination


    5 Interacting proteins for UBE2Z (ENSP000003742593 Q9H8322) via UniProtKB, MINT, and/or STRING

    InteractantInteraction Details
    GeneCardExternal ID(s)
    LPLP068582MINT-63417
    PLEKHF2Q9H8W42MINT-67937
    UBE2HENSP000003478363STRING (score=.835)
    TCTN3ENSP000002659933STRING (score=.706)
    --ENSP000003736153STRING (score=.803)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006915 apoptosis IEA--
    GO:0019941 modification-dependent protein catabolic process IEA--
    GO:0043687 post-translational protein modification IEA--
    GO:0051246 regulation of protein metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBE2Z

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2Z)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2Z)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_023079

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2Z  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2Z  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_023079  

    REFSEQ mRNAs for UBE2Z gene: 

    NM_023079.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_023079  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_023079
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_023079
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_023079 

    Additional cDNA sequence: 

    AB025426.1 AK023917.1 AK024030.1 AK091644.1 AK122770.1 AK130303.1 AK296865.1 AL713782.1 
    BC015169.1 BC015890.1 CR457322.1 EF623992.1 

    23 DOTS entries:

    DT.456081  DT.101966986  DT.100826843  DT.97851377  DT.99957834  DT.100826844  DT.120911283  DT.120911314 
    DT.120911417  DT.91759275  DT.91759295  DT.101984794  DT.102844558  DT.120911414  DT.120911425  DT.75101708 
    DT.75149705  DT.91759293  DT.95223440  DT.100649701  DT.219192  DT.95225735  DT.99942923 

    24/436 AceView cDNA sequences (see all 436 ):

    AK122770 CA952898 BQ130583 CD672198 CD518800 AA845421 T87836 AI669613 
    AW103048 AA445998 BM738472 BE501620 BM803464 BM272571 AA460943 AW006025 
    CA422112 BE857552 CA417956 AI095613 CB321809 CB529140 AW071453 BI757130 

    highest scoring ESTs for UBE2Z:

    AI027524 AA027972 AA027973 AA029054 AA029157 AA043352 AA043353 AA100618 AA100619 AA147920 

    Unigene Cluster for UBE2Z:

    Ubiquitin-conjugating enzyme E2Z
    Hs.514297  [show with all ESTs]
    Unigene Representative Sequence: AK091644


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2Z

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7a · 7b
    SP1:        -     -           -           -                                       
    SP2:                          -     -     -                                       
    SP3:                                      -                                       
    SP4:                                                                              
    SP5:                          -           -                                       

    About this scheme

    ECgene alternative splicing isoforms for UBE2Z

    3 Ensembl transcripts including schematic representations:
    ENST00000360943  ENST00000405215  ENST00000389608  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBE2Z expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBE2Z

    1 / 2 / 3

    8 probe-sets matching UBE2Z gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    44729_at2, 3 U95-B 1 1.00 1.00 0.41 1.37 AI027524 1.00 1.00 1.00 1

    53753_at2, 3 U95-C 1 0.94 1.00 0.73 0.64 AA147920 0.80 1.00 0.91 1

    217750_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_023079 0.60 1.00 0.82 1

    222395_s_at2, 3 U133-B 1 1.00 1.00 -- -- BE544096 0.80 1.00 0.91 1

    236107_at2, 3 U133-B 1 1.00 1.00 -- -- AA147920 0.80 1.00 0.91 1

    217750_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222395_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    236107_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TCTGGCATAG

    SOURCE GeneReport for Unigene cluster: Hs.514297

    UniProtKB/Swiss-Prot: UBE2Z_HUMAN, Q9H832
    Tissue specificity: Widely expressed. Highly in placenta, pancreas, spleen and testis

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBE2Z gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    UBE2Z1   -- ubiquitin-conjugating enzyme E2Z 98.76(n)
    99.07(a)
    455196  XM_511942.2  XP_511942.2 
    mouse
    (Mus musculus)
    Ube2z1, 5 11 (55.80 cM)5
    ubiquitin-conjugating enzyme E2Z (putative)1, 5 94.26(n)1
    99.44(a)1
    2684701  NM_172300.31  NP_758504.31 
     AA1682065  AA4747795  (see all 31)
    chicken
    (Gallus gallus)
    UBE2Z1   -- ubiquitin-conjugating enzyme E2Z (putative) 79.01(n)
    81.05(a)
    419991  XM_418113.2  XP_418113.1 
    zebrafish
    (Danio rerio)
    Dr.288572   -- Transcribed sequence with moderate similarity to protein more 77.78(n)  CF999866.1 
    African clawed frog
    (Xenopus laevis)
    MGC691552   -- hypothetical protein MGC69155 79.33(n)  BC059343.1 
    About this table        Species with no ortholog for UBE2Z

    ENSEMBL Gene Tree for UBE2Z
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBE2Z gene
    UBE2O2  BIRC62  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/135 NCBI SNPs in UBE2Z are shown (see all 135 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs155631,2
    A,C,F,H44360192(-) TTTACC/TTTCTG 1 -- ut3117Minor allele frequency- T:0.40MN EU EA WA NA 3016
    rs10578971,2
    A,C,F,H44360508(+) GATTTT/GTGGGG 1 -- ut31 ese38Minor allele frequency- G:0.40MN NA EU EA WA 736
    rs20881381,2
    A,C,F,H44359722(-) TGATAT/CGGGAT 1 -- ut31 ese310Minor allele frequency- C:0.40EA NA EU WA MN 3198
    rs72212151,2
    F,H44360793(+) TCCAGA/GTTTAT 1 -- ut314Minor allele frequency- G:0.01EU EA WA 418
    --
    rs10579021,2
    A,F44360587(+) CCTTCC/TGCCTC 1 -- ut31 ese3 trp31Minor allele frequency- T:0.43MN 184
    --
    rs126016721,2
    C,F44361492(+) TCACCT/CAGGCC 1 -- ng313Minor allele frequency- C:0.42NA 142
    rs116523361,2
    C,H44340590(+) CTCCGC/TTACTT 1 -- ng514Minor allele frequency- T:0.00EU EA WA 396
    --
    rs116572381,2
    A,C44340633(+) CCATCA/GCGGCC 1 -- ng510--------
    rs37446111,2
    H44360380(+) TCACAA/GTAAGG 1 -- ut31 ese34Minor allele frequency- G:0.00EU EA WA 392
    --
    rs99021311,2
    A44339041(+) ccggcA/Taattt 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for UBE2Z (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611362

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for UBE2Z gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 65264 HGNC: 25847 AceView: FLJ13855 Ensembl:ENSG00000159202 euGenes: HUgn65264
    ECgene: UBE2Z H-InvDB: UBE2Z
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBE2Z:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for UBE2Z Antibodies for UBE2Z
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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