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UBE2W Gene

protein-coding   GIFtS: 49

GC08M074866
ubiquitin-conjugating enzyme E2W (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.3.2.19 3
FLJ11011 1, 2
hUBC-16 2
Descriptions
Ubiquitin carrier protein W 3
Ubiquitin-protein ligase W 3
ubiquitin-conjugating enzyme 16 2
ubiquitin-conjugating enzyme E2W (putative) 2
External Ids
HGNC: 256161
Entrez Gene: 552842
UniProtKB: Q96B023
Ensembl: ENSG000001043437
Search outside databases for aliases for UBE2W gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: UBE2W_HUMAN, Q96B02
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2W gene  

Entrez Gene cytogenetic band: 8q21.11   Ensembl cytogenetic band:  8q21.11   HGNC cytogenetic band: 8q21.11

UBE2W Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M074866:     (about GC identifiers)

Start:
74,865,394 bp from pter
End:
74,953,664 bp from pter
Size:
88,271 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_008183.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UBE2W_HUMAN, Q96B02 (See protein sequence)
Recommended Name: Probable ubiquitin-conjugating enzyme E2 W  
Size: 151 amino acids; 17331 Da
Subunit: Homodimer
PDB structures from and Proteopedia :
2A7L (3D)    
Secondary accessions: Q9H823 Q9HAG6 Q9NV07
Alternative splicing: 2 isoforms:  Q96B02-1   Q96B02-2   

REFSEQ proteins (2 alternative transcripts): 
NP_001001481.1  NP_060769.3  


ENSEMBL proteins: 
ENSP00000351607 ENSP00000382398 ENSP00000355382 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (UBE2W)
Human Recombinant Proteins from Abnova (UBE2W)
              OriGene Purified Recombinant Human Protein: UBE2W 


Antibodies for UBE2W: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for UBE2W
Browse R&D Systems for Antibodies
Antibodies from Abcam (UBE2W), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2W)
Novus Biologicals Antibodies for UBE2W

Assays for UBE2W: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like


   GeneDecks  UBE2W for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q96B02

ProtoNet protein and cluster: Q96B02

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UBE2W_HUMAN, Q96B02
Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2W)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2W)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_018299

              Applied Biosystems Silencer® siRNAs for UBE2W

              Sigma-Aldrich siRNA and siRNA Panels for UBE2W  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2W  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for UBE2W
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001001481
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001001481
                                 untagged cDNA clones in CMV expression vector (see all 4): BC010900 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001001481

UniProtKB/Swiss-Prot: UBE2W_HUMAN, Q96B02
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
N-ubiquityllysine
Enzyme Number (IUBMB): EC 6.3.2.19 

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004842 ubiquitin-protein ligase activity IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Kegg Pathway  (Kegg details for UBE2W):
 hsa04120 Ubiquitin mediated proteolysis

   GeneDecks  UBE2W for the pathways selected above  
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UniProtKB/Swiss-Prot: UBE2W_HUMAN, Q96B02
Pathway: Protein modification; protein ubiquitination

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0019941 modification-dependent protein catabolic process IEA--
GO:0043687 post-translational protein modification IEA--
GO:0051246 regulation of protein metabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2W

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2W)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2W)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_018299

              Sigma-Aldrich siRNA and siRNA Panels for UBE2W  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2W  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001001481  NM_018299  

REFSEQ mRNAs for UBE2W gene (2 alternative transcripts): 

NM_001001481.1   NM_018299.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001001481  NM_018299  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001001481
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001001481
                                 untagged cDNA clones in CMV expression vector (see all 4): BC010900 

Additional cDNA sequence: 

AK001873.1 AK021735.1 AK024050.1 AK225554.1 AK295792.1 AL833723.1 AY948289.1 BC010900.1 
BC016326.1 BX538145.1 BX648427.1 CR457275.1 CR597814.1 CR609430.1 

8 DOTS entries:

DT.100821509  DT.212257  DT.100640421  DT.102842  DT.95375728  DT.212259  DT.100821506  DT.65287833 

24/446 AceView cDNA sequences (see all 446 ):

AU133836 NM_018299 CA311807 BC071766 AI040886 D25755 AV709525 AK002152 
BX399252 BQ082747 AI290456 AI741718 BQ000367 CR597814 AA252578 BQ220156 
AW235263 BM979176 AL705155 NM_001001482 Z40600 BX538145 Z40558 AI768561 

highest scoring ESTs for UBE2W:

AA620611 AI625741 AK001873 AK021735 AK024050 AL710166 AU143505 BC016326 BG426710 BI547963 

Unigene Cluster for UBE2W:

Ubiquitin-conjugating enzyme E2W (putative)
Hs.597486  [show with all ESTs]
Unigene Representative Sequence: NM_001001481


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2W

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b
SP1:                                                      
SP2:              -                                       
SP3:              -           -                           

About this scheme

ECgene alternative splicing isoforms for UBE2W

3 Ensembl transcripts including schematic representations:
ENST00000358757  ENST00000399473  ENST00000362030  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2W expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2W

1 / 2 / 3

11 probe-sets matching UBE2W gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
59735_at2, 3 U95-B 1 1.00 1.00 0.86 0.99 AI625741 0.80 0.88 0.84 1

60199_at2, 3 U95-C 1 1.00 1.00 0.68 0.74 AA453924 0.60 0.83 0.73 1

46903_at2, 3 U95-B 1 1.00 1.00 0.77 1.11 AA620611 0.80 0.88 0.84 1

60791_at2, 3 U95-C 1 1.00 1.00 0.42 1.17 AI828160 0.60 0.83 0.73 1

218521_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018299 0.60 0.83 0.73 1

222657_s_at2, 3 U133-B 1 1.00 1.00 -- -- AK024050 0.80 0.75 0.78 1

222656_at2, 3 U133-B 1 1.00 1.00 -- -- AI625741 0.80 0.88 0.84 1

218521_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222657_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222656_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1555217_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  UBE2W for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.597486
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for UBE2W gene from 5/12 species (see all 12 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
UBE2W1   -- ubiquitin-conjugating enzyme E2W (putative) 97.13(n)
100(a)
486999  XM_544128.2  XP_544128.2 
chimpanzee
(Pan troglodytes)
UBE2W1   -- ubiquitin-conjugating enzyme E2W (putative) 99.78(n)
100(a)
464234  XM_519809.2  XP_519809.2 
cow
(Bos taurus)
UBE2W1   -- ubiquitin-conjugating enzyme E2W (putative) 98.23(n)
100(a)
613423  XM_863948.2  XP_869041.1 
mouse
(Mus musculus)
Ube2w1, 5 15
ubiquitin-conjugating enzyme E2W (putative)1, 5 94.48(n)1
99.34(a)1
667991  NM_025773.21  NP_080049.11 
 AI4488615  AI8456335  (see all 18)
chicken
(Gallus gallus)
UBE2W1   -- ubiquitin-conjugating enzyme E2W (putative) 90.95(n)
99.34(a)
420185  XM_418298.2  XP_418298.1 
About this table        Species with no ortholog for UBE2W

ENSEMBL Gene Tree for UBE2W
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for UBE2W gene
UBE2R22  CDC342  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/490 NCBI SNPs in UBE2W are shown (see all 490 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 102)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 8 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs169387301,2
C,F,H74867704(+) AGTGAC/TTTTTG 2 -- ut31 ese311Minor allele frequency- T:0.17NA EU EA WA 966
--
rs172242891,2
C,F,H74865687(+) ATTATC/TCACCA 2 -- ut31 ese37Minor allele frequency- T:0.06NA EU EA WA 560
rs169387291,2
C,F,H74865041(+) ACATCA/TGTGGG 2 -- ng517Minor allele frequency- T:0.23NA EU EA WA 556
rs78369141,2
A,H74955273(+) TTCCTG/ATGGAA 2 -- ng314Minor allele frequency- A:0.00EU EA WA 412
rs78327051,2
A,H74954724(+) TACCAG/AAATGA 2 -- ng314Minor allele frequency- A:0.00EU EA WA 418
rs78366791,2
A,H74955280(+) GGAAAC/AAGTAG 2 -- ng314Minor allele frequency- A:0.00EU EA WA 418
rs78366641,2
A,H74955235(+) TTTACC/TGTCCA 2 -- ng314Minor allele frequency- T:0.00EU EA WA 418
rs78366271,2
A,H74955075(+) AATCAG/TCCAGA 2 -- ng31 tfbs34Minor allele frequency- T:0.00EU EA WA 418
rs78164091,2
A,H74954869(+) TGTGTT/CCAAAA 2 -- ng313Minor allele frequency- C:0.00EA WA 298
rs169387281,2
F,H74864959(+) GTAGAA/TTCCCT 2 -- ng517Minor allele frequency- T:0.05NA EU EA WA 560
About this table

HapMap Linkage Disequilibrium images for UBE2W (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for UBE2W gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 55284 HGNC: 25616 AceView: STAU2andFLJ11011 Ensembl:ENSG00000104343 euGenes: HUgn55284
ECgene: UBE2W H-InvDB: UBE2W
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2W:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for UBE2W-related products

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 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for UBE2W Antibodies for UBE2W
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for UBE2W Browse small molecules at Sigma-Aldrich
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 Tagged/untagged cDNA clones
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for UBE2W




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for UBE2W

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