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UBE2V2 Gene

protein-coding   GIFtS: 65

GC08P049083
ubiquitin-conjugating enzyme E2 variant 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DDVIT1 2
DDVit-1 1, 2
EDAF-1 2
EDPF-1 1, 2, 3
EDPF1 2
MMS2 1, 2, 3
UEV-2 1, 2
UEV2 2, 3
Descriptions
1 alpha,25-dihydroxyvitamin D3-inducible 2
DDVit 1 3
Enterocyte differentiation-associated factor EDAF-1 3
Enterocyte differentiation-promoting factor 3
Vitamin D3-inducible protein 3
enterocyte differentiation promoting factor 2
methyl methanesulfonate sensitive 2, S. cerevisiae, homolog
of 2
ubiquitin-conjugating enzyme E2 variant 2 2
ubiquitin-conjugating enzyme E2v2 2
External Ids
HGNC: 124951
Entrez Gene: 73362
UniProtKB: Q158193
Ensembl: ENSG000001691397
Search outside databases for aliases for UBE2V2 gene

Previous GC identifers: GC08P048012 GC08P048925 GC08P048644 GC08P048970

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for UBE2V2:
Ubiquitin-conjugating enzyme E2 variant proteins constitute a distinct subfamily within the E2
protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the
conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded
by this gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2
gene product. It may be involved in the differentiation of monocytes and enterocytes. [provided by
RefSeq]

UniProtKB/Swiss-Prot: UB2V2_HUMAN, Q15819
Function: Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the
synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of
poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates
transcriptional activation of target genes. Plays a role in the control of progress through the
cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes
to the survival of cells after DNA damage

Gene Wiki entry for UBE2V2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2V2 gene  

Entrez Gene cytogenetic band: 8q11.21   Ensembl cytogenetic band:  8q11.21   HGNC cytogenetic band: 8q11.21

UBE2V2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P049083:     (about GC identifiers)

Start:
49,083,548 bp from pter
End:
49,137,007 bp from pter
Size:
53,460 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000008.9  NT_008183.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UB2V2_HUMAN, Q15819 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 variant 2  
Size: 145 amino acids; 16363 Da
Subunit: Heterodimer with UBE2N. Binds CHFR
PDB structures from and Proteopedia :
1J74 (3D)    1J7D (3D)    1ZGU (3D)    

REFSEQ proteins: NP_003341.1  

ENSEMBL proteins: 
ENSP00000326473 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Millipore Purified and/or Recombinant UBE2V2 Protein
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Enzo Life Sciences proteins for UBE2V2
Recombinant Proteins from Abcam (UBE2V2 +UBE2V1, UEV2)
Human Recombinant Proteins from Abnova (UBE2V2)
              OriGene Purified Recombinant Human Protein: UBE2V2 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IDA16129784
GO:0005737 cytoplasm TAS16129784
GO:0031372 UBC13-MMS2 complex IDA16129784
About this table

Antibodies for UBE2V2: 
Invitrogen Antibodies for UBE2V2
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (UBE2V2 +UBE2V1, UEV2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2V2)
Novus Biologicals Antibodies for UBE2V2

Assays for UBE2V2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Enzo Life Sciences assays for UBE2V2

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like


   GeneDecks  UBE2V2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q15819

ProtoNet protein and cluster: Q15819

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UB2V2_HUMAN, Q15819
Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2V2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2V2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003350

              Applied Biosystems Silencer® siRNAs for UBE2V2

              Sigma-Aldrich siRNA and siRNA Panels for UBE2V2  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2V2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for UBE2V2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003350
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003350
                                 untagged cDNA clone in CMV expression vector: NM_003350 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_003350

UniProtKB/Swiss-Prot: UB2V2_HUMAN, Q15819
Function: Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the
synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of
poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates
transcriptional activation of target genes. Plays a role in the control of progress through the
cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes
to the survival of cells after DNA damage
Induction: Up-regulated in cultured fresh blood cells upon treatment with vitamin D3

Genatlas biochemistry entry for UBE2V2:
ubiquitin conjugating enzyme E2 variant 2,expressed in monocytes and in some cancer
lines,putatively involved in the control of differentiation

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI11473255 16786304
GO:0019787 small conjugating protein ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

2 Invitrogen iPath™ Online BioAtlas - Pathways for UBE2V2 (Maps provided by GeneGo):
 T cell receptor signaling pathway
 TCR and CD28 co-stimulation in activation of NF-kB

   GeneDecks  UBE2V2 for the pathways selected above  
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2 Millipore Pathways for UBE2V2
 Common schema of ubiquitination
 Immune response T cell receptor signaling pathway

   GeneDecks  UBE2V2 for the pathways selected above  
About GeneDecksing

2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2V2  (Your Favorite Gene powered by Ingenuity) 
 Hypoxia Signaling in the Cardiovascular System
 Protein Ubiquitination Pathway

   GeneDecks  UBE2V2 for the pathways selected above  
About GeneDecksing

 Gene Network CentralTM Interacting Genes and Proteins Network for  UBE2V2 


5/19 Interacting proteins for UBE2V2 (Q158191, 2 ENSP000003264733) via UniProtKB, MINT, and/or STRING (see all 19 )
InteractantInteraction Details
GeneCardExternal ID(s)
UBE2NP610881, ENSP000003161763EBI-714329, EBI-1052908 STRING (score=.999)
UBC35 UBC35 (xeno)Q94A971EBI-714329, EBI-994120
IGSF21Q96ID52MINT-64269
MOBKL3Q9Y3A32MINT-65831
PCNAENSP000003684383STRING (score=.984)
About this table

5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000209 protein polyubiquitination TAS10089880
GO:0000729 DNA double-strand break processing IMP17349954
GO:0006282 regulation of DNA repair TAS10089880
GO:0008283 cell proliferation TAS9418904
GO:0019941 modification-dependent protein catabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2V2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2V2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2V2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003350

              Sigma-Aldrich siRNA and siRNA Panels for UBE2V2  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2V2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003350  

REFSEQ mRNAs for UBE2V2 gene: 

NM_003350.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003350  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003350
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_003350
                                 untagged cDNA clone in CMV expression vector: NM_003350 

Additional cDNA sequence: 

AF049140.1 AK094617.1 AK313447.1 BC007051.1 BC016332.1 BC016710.1 BC028673.1 BC062418.1 
BT006744.1 CR407628.1 CR603464.1 CR603543.1 U62136.2 X98091.1 

24/25 DOTS entries (see all 25 ):

DT.92468578  DT.99975199  DT.92468536  DT.310202  DT.91999321  DT.92468530  DT.100827881  DT.100827885 
DT.92468535  DT.100827883  DT.95284972  DT.100827884  DT.100827877  DT.121485744  DT.121485844  DT.121485861 
DT.92468588  DT.95284962  DT.95284984  DT.100827873  DT.121485750  DT.95284971  DT.95284988  DT.99980382 

24/1109 AceView cDNA sequences (see all 1109 ):

BE698527 AI055876 AV708041 AW246621 BE504258 BM833841 BM719387 BF589541 
AW024676 CA441431 AI253060 BU567372 BE698521 BU677186 BP347509 AI202679 
F11561 BU508785 AI763271 BU928473 CB127259 AA776914 BM477255 BG533577 

highest scoring ESTs for UBE2V2:

AF049140 AA071363 AA102842 AA102843 AA129335 AA148454 AA148455 AA160935 AA164942 AA195549 

Unigene Cluster for UBE2V2:

Ubiquitin-conjugating enzyme E2 variant 2
Hs.491695  [show with all ESTs]
Unigene Representative Sequence: AK094617


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2V2

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c
SP1:                                                
SP2:                          -                     
SP3:              -           -                     

About this scheme

ECgene alternative splicing isoforms for UBE2V2

1 Ensembl transcript including schematic representation:
ENST00000324746  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2V2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2V2

1 / 2 / 3

3 probe-sets matching UBE2V2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

37750_at2, 3 U95-A 1 0.56 1.00 1.00 1.00 AF049140 1.00 1.00 1.00 1

209096_at2, 3 U133-A 1 1.00 1.00 -- -- U62136 0.80 1.00 0.91 1

209096_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CATTTAAGTT

SOURCE GeneReport for Unigene cluster: Hs.491695

Expression variation in blood from EXPOLDB for UBE2V2

UniProtKB/Swiss-Prot: UB2V2_HUMAN, Q15819
Tissue specificity: Detected in placenta, colon, liver and skin. Detected at very low levels in
most tissues

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for UBE2V2 gene from 5/18 species (see all 18 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
UBE2V21   -- ubiquitin-conjugating enzyme E2 variant 2 96.55(n)
97.24(a)
612943  XM_847910.1  XP_853003.1 
chimpanzee
(Pan troglodytes)
UBE2V21   -- ubiquitin-conjugating enzyme E2 variant 2 99.52(n)
100(a)
740505  XM_001142548.1  XP_001142548.1 
cow
(Bos taurus)
UBE2V21   -- ubiquitin-conjugating enzyme E2 variant 2 95.86(n)
100(a)
286803  NM_001034042.1  NP_001029214.1 
rat
(Rattus norvegicus)
Ube2v21   -- ubiquitin-conjugating enzyme E2 variant 2 94.48(n)
99.31(a)
287927  NM_183052.1  NP_898875.1 
mouse
(Mus musculus)
Ube2v21, 5 165
ubiquitin-conjugating enzyme E2 variant 21, 5 95.17(n)1
98.62(a)1
706201  NM_023585.31  NP_076074.21 
 AF3038285  AI8483155  (see all 25)
About this table        Species with no ortholog for UBE2V2

ENSEMBL Gene Tree for UBE2V2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for UBE2V2 gene
TMEM1892  

Pseudogenes
8 related pseudogenes


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/182 NCBI SNPs in UBE2V2 are shown (see all 182 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 21)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 8 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs108088971,2
A,C,F,H49137169(+) GAAAAG/TTCATT 1 -- ng3117Minor allele frequency- T:0.25EU EA WA NA NS 1382
--
rs285783311,2
C,F49083096(+) AGTGGC/TGCAAT 1 -- ng515Minor allele frequency- T:0.24NS 190
rs285424801,2
C,F49083499(+) GCGGCG/TCGCTC 1 -- ng515Minor allele frequency- T:0.03NS 178
--
rs286995691,2
C,F49083224(+) GAGACG/AGGGTT 1 -- ng515Minor allele frequency- A:0.03NS 188
--
rs115577891,2
H49125030(+) AGCTCC/TTCCGT 1 P/L mis14Minor allele frequency- T:0.00EU EA WA 406
--
rs148901,2
H49118247(+) ATGAAG/CATATG 1 H/D mis14Minor allele frequency- C:0.00EU EA WA 402
--
rs115577761,2
H49118236(+) CCTTGA/GAGATG 1 E/G mis14Minor allele frequency- G:0.00EU EA WA 406
--
rs115577811,2
H49135832(+) ATGGCA/GAAATT 1 Q/R mis14Minor allele frequency- G:0.00EU EA WA 406
--
rs115577781,2
H49125050(+) TTGTAA/GCAAAA 1 T/A mis14Minor allele frequency- G:0.00EU EA WA 394
rs23826371,2
H49137321(+) ATTTTG/TTCAAG 1 -- ng314Minor allele frequency- T:0.00EU EA WA 416
About this table

HapMap Linkage Disequilibrium images for UBE2V2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 603001

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/28 PubMed articles for UBE2V2 gene (see all 28 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 7336 HGNC: 12495 AceView: UBE2V2 Ensembl:ENSG00000169139 euGenes: HUgn7336
ECgene: UBE2V2 H-InvDB: UBE2V2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for UBE2V2 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2V2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Lentivirus-delivered shRNAs for UBE2V2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search for Antibodies & Assays

 Recombinant Proteins
(UBE2V2 +UBE2V1, UEV2)
 Antibodies (UBE2V2 +UBE2V1, UEV2)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for UBE2V2




 Assays/Proteins for UBE2V2
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for UBE2V2

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