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UBE2U Gene

protein-coding GIFtS: 49

GC01P064382

ubiquitin-conjugating enzyme E2U (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
EC 6.3.2.19 ^³
MGC35130 ^¹, ²
OTTHUMP00000010575 ^²
RP4-636O23.1 ^²

Descriptions
Ubiquitin carrier protein U ^³
Ubiquitin-protein ligase U ^³
ubiquitin-conjugating enzyme E2U (putative) ^²

External Ids
HGNC: 28559¹
Entrez Gene: 148581²
UniProtKB: Q5VVX9³
Ensembl: ENSG00000177414⁷

Search outside databases for aliases for UBE2U gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: UBE2U_HUMAN, Q5VVX9

Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the UBE2U gene

Entrez Gene cytogenetic band: 1p31.3 Ensembl cytogenetic band: 1p31.3 HGNC cytogenetic band: 1p31.3

UBE2U Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01P064382: (about GC identifiers)

Start:	64,442,078 bp from pter
End:	64,505,479 bp from pter
Size:	63,402 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000001.9 NT_032977.8

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UBE2U_HUMAN, Q5VVX9 (See protein sequence)

Recommended Name: Ubiquitin-conjugating enzyme E2 U

Size: 321 amino acids; 37741 Da

PDB structures from and Proteopedia :

1YRV (3D)

Secondary accessions: Q8N1D4

Alternative splicing: 2 isoforms: Q5VVX9-1 Q5VVX9-2

REFSEQ proteins: NP_689702.1

ENSEMBL proteins:

ENSP00000360116 ENSP00000360117

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (UBE2U)
	Human Recombinant Proteins from Abnova (UBE2U)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for UBE2U:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for UBE2U
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (UBE2U), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (UBE2U)
	Novus Biologicals Antibodies for UBE2U

Assays for UBE2U:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q5VVX9

ProtoNet protein and cluster: Q5VVX9

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UBE2U_HUMAN, Q5VVX9

Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (UBE2U)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (UBE2U)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152489

Applied Biosystems Silencer® siRNAs for UBE2U

Sigma-Aldrich siRNA and siRNA Panels for UBE2U

Sigma-Aldrich shRNA Panels and shRNA for UBE2U

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for UBE2U
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_152489
Myc/DDK tagged cDNA clone in CMV expression vector: NM_152489
untagged cDNA clone in CMV expression vector: NM_152489

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_152489

UniProtKB/Swiss-Prot: UBE2U_HUMAN, Q5VVX9

Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein

N-ubiquityllysine

Enzyme Number (IUBMB): EC 6.3.2.19

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004842	ubiquitin-protein ligase activity	IEA	--
GO:0016874	ligase activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: UBE2U_HUMAN, Q5VVX9

Pathway: Protein modification; protein ubiquitination

3 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0019941	modification-dependent protein catabolic process	IEA	--
GO:0043687	post-translational protein modification	IEA	--
GO:0051246	regulation of protein metabolic process	IEA	--

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2U

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (UBE2U)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (UBE2U)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152489

Sigma-Aldrich siRNA and siRNA Panels for UBE2U

Sigma-Aldrich shRNA Panels and shRNA for UBE2U

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_152489

REFSEQ mRNAs for UBE2U gene:

NM_152489.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_152489

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_152489
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_152489
                                 untagged cDNA clone in CMV expression vector: NM_152489

Additional cDNA sequence: BC029895.1

3 DOTS entries:

DT.100015008 DT.70103714 DT.70100712

21 AceView cDNA sequences:

BQ022574 AI198955 BC029895 NM_152489 AI538316 AI208348 AW136185 AA626174

BU193579 AI478778 AI377288 BX116499 BI825812 BG773201 AI655911 BG773116

BI462751 AI765312 R20805 BU561173 R20919

highest scoring ESTs for UBE2U:

AA626174 AI377288 BC029895 R20805 AI198955 AI208348 AI478778 AI538316 AI655911 AI765312

Unigene Cluster for UBE2U:

Ubiquitin-conjugating enzyme E2U (putative)

Hs.388746 [show with all ESTs]

Unigene Representative Sequence: BC029895

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000371076 ENST00000371077

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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UBE2U expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for UBE2U

¹ / ² / ³

6 probe-sets matching UBE2U gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
43199_at²^{, 3}	U95-B	1	1.00	1.00	-0.06	0.81	AA626174	0.80	1.00	0.91	1
51308_at²^{, 3}	U95-B	1	0.75	1.00	-0.29	0.64	R20805	0.80	1.00	0.91	1
83846_at²^{, 3}	U95-E	1	0.69	1.00	1.00	1.00	AI377288	0.80	1.00	0.91	1
242392_at²^{, 3}	U133-B	1	0.27	1.00	--	--	AI765312	0.60	1.00	0.82	1
1553776_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
242392_at²	U133Plus2	1	0.27	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: AGCTTCAGAG

SOURCE GeneReport for Unigene cluster: Hs.388746

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for UBE2U gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	UBE2U¹	--	ubiquitin-conjugating enzyme E2U (putative)	83.92(n) 74.78(a)	609932 XM_847284.1 XP_852377.1
chimpanzee (Pan troglodytes)	UBE2U¹	--	ubiquitin-conjugating enzyme E2U (putative)	98.82(n) 98.23(a)	469342 XM_524727.2 XP_524727.2
cow (Bos taurus)	UBE2U¹	--	ubiquitin-conjugating enzyme E2U (putative)	84.99(n) 76.16(a)	784986 XM_001253136.1 XP_001253137.1
mouse (Mus musculus)	Ube2u⁵	4	ubiquitin-conjugating enzyme E2U (putative)	--	BC172031

About this table Species with no ortholog for UBE2U

ENSEMBL Gene Tree for UBE2U

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/241 NCBI SNPs in UBE2U are shown (see all 241 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 92)

AB	SNP ID	Valid	Chr 1 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs2644497^1,2	A,C,F,H	64441154(+)	`AGGCTC/TTGTAG`	1	--	ng5¹		4		EU EA WA	414
	rs10789152^1,2	A,C,F,H	64482479(+)	`TCTTAA/GCCACT`	1	--	ut3¹		4		EU EA WA	418
	rs2806529^1,2	A,C,F,H	64442155(+)	`GTTATA/TTCCCA`	1	--	ut5¹ ese³		4		EU EA WA	402
	rs7532933^1,2	C,F,H	64449040(+)	`CTGTAT/CAGACT`	1	T/I	mis¹ ese³		4		EU EA WA	412
--	rs4915950^1,2	A,C	64440543(+)	`taaaaA/Taaata`	1	--	ng5¹ tfbs³		0	--	--	--	--
--	rs11809954^1,2	C	64440593(+)	`CTCTCC/TTGTTT`	1	--	ng5¹		0	--	--	--	--
--	rs2935206^1,2	C	64440209(+)	`ttcttC/Ttgcct`	1	--	ng5¹		0	--	--	--	--
	rs34327217^1,2	F	64445120(-)	`TTTGGA/GTGAAA`	1	H/H	syn¹		1		NA	78
	rs35834004^1,2	F	64445114(-)	`TGAAAC/TGGAAT`	1	P/P	syn¹		1		NA	78
	rs35112334^1,2	F	64445066(-)	`TCCGAT/CGTAAA`	1	T/T	syn¹		1		NA	76

About this table

HapMap Linkage Disequilibrium images for UBE2U (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

4 PubMed articles for UBE2U gene:

The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)^{3, 4} Gregory S.G.... Bentley D.R. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)^{2, 3} Strausberg R.L....Marra M.A. (2002)
Autoantibodies against ubiquitination factor E4A (UBE4A) are associated with severity of Crohn's disease. (PubMed id 18069675)¹ Sakiyama T....Tsubouchi H. (2008)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 148581	HGNC: 28559	AceView: MGC35130	Ensembl:ENSG00000177414	euGenes: HUgn148581
ECgene: UBE2U	H-InvDB: UBE2U

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for UBE2U
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for UBE2U:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for UBE2U-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for UBE2U		Antibodies for UBE2U
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for UBE2U		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (UBE2U)
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	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

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UBE2U Gene

protein-coding GIFtS: 49

Aliases &
Descriptions
for UBE2U

Summaries
for UBE2U

Genomic Location
for UBE2U

Proteins
for UBE2U

Protein Domains/
Families
for UBE2U

Gene Function
for UBE2U

Pathways & Interactions
for UBE2U

Drugs & Compounds
for UBE2U

Transcripts
for UBE2U

Expression
for UBE2U

Orthologs
for UBE2U

Paralogs
for UBE2U

SNPs/Variants
for UBE2U

Disorders & Mutations
for UBE2U

Medical News
for UBE2U

Publications
for UBE2U

Search for UBE2U

Genome Databases showing UBE2U

Other Databases showing UBE2U

Specialized Databases showing UBE2U

Licensable Technologies
for UBE2U

Services
for UBE2U

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

UBE2U Gene

protein-coding GIFtS: 49

Aliases & Descriptionsfor UBE2U

Summariesfor UBE2U

Genomic Locationfor UBE2U

Proteinsfor UBE2U

Protein Domains/Familiesfor UBE2U

Gene Functionfor UBE2U

Pathways & Interactionsfor UBE2U

Drugs & Compoundsfor UBE2U

Transcriptsfor UBE2U

Expression for UBE2U

Orthologsfor UBE2U

Paralogsfor UBE2U

SNPs/Variantsfor UBE2U

Disorders & Mutationsfor UBE2U

Medical Newsfor UBE2U

Publicationsfor UBE2U

Search for UBE2U

Genome Databases showing UBE2U

Other Databases showing UBE2U

Specialized Databases showing UBE2U

Licensable Technologiesfor UBE2U

Servicesfor UBE2U

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for UBE2U

Summaries
for UBE2U

Genomic Location
for UBE2U

Proteins
for UBE2U

Protein Domains/
Families
for UBE2U

Gene Function
for UBE2U

Pathways & Interactions
for UBE2U

Drugs & Compounds
for UBE2U

Transcripts
for UBE2U

Expression
for UBE2U

Orthologs
for UBE2U

Paralogs
for UBE2U

SNPs/Variants
for UBE2U

Disorders & Mutations
for UBE2U

Medical News
for UBE2U

Publications
for UBE2U

Licensable Technologies
for UBE2U

Services
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