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UBE2T Gene

protein-coding   GIFtS: 51

GC01M200567
ubiquitin-conjugating enzyme E2T (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 6.3.2.19 3
HSPC150 1, 2, 5
OTTHUMP00000038808 2
PIG50 2
Descriptions
HSPC150 protein similar to ubiquitin-conjugating enzyme 2
Ubiquitin carrier protein T 3
Ubiquitin-protein ligase T 3
ubiquitin conjugating enzyme 2
ubiquitin-conjugating enzyme E2T 2
ubiquitin-conjugating enzyme E2T (putative) 2
External Ids
HGNC: 250091
Entrez Gene: 290892
UniProtKB: Q9NPD83
Ensembl: ENSG000000771527
Search outside databases for aliases for UBE2T gene

Previous GC identifer: GC01M199033

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for UBE2T:
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins.
Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating
enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3)
(Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

UniProtKB/Swiss-Prot: UBE2T_HUMAN, Q9NPD8
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2T gene  

Entrez Gene cytogenetic band: 1q32.1   Ensembl cytogenetic band:  1q32.1   HGNC cytogenetic band: 1q32.1

UBE2T Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M200567:     (about GC identifiers)

Start:
200,567,408 bp from pter
End:
200,577,707 bp from pter
Size:
10,300 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: UBE2T_HUMAN, Q9NPD8 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 T  
Size: 197 amino acids; 22521 Da
PDB structures from and Proteopedia :
1YH2 (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_054895.1  

    ENSEMBL proteins: 
    ENSP00000356243 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (UBE2T)
                  OriGene Purified Recombinant Human Protein: UBE2T 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA18029348
    GO:0005730 nucleolus IDA18029348
    GO:0005737 cytoplasm IDA18029348
    About this table

    Antibodies for UBE2T: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for UBE2T
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Monoclonal and Polyclonal Antibodies from Abnova (UBE2T)
    Novus Biologicals Antibodies for UBE2T

    Assays for UBE2T: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugat/RWD-like


       GeneDecks  UBE2T for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NPD8

    ProtoNet protein and cluster: Q9NPD8

    1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UBE2T_HUMAN, Q9NPD8
    Similarity: Belongs to the ubiquitin-conjugating enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2T)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2T)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014176

                  Applied Biosystems Silencer® siRNAs for UBE2T

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2T  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2T  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBE2T
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014176
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014176
                                     untagged cDNA clone in CMV expression vector: NM_014176 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014176

    UniProtKB/Swiss-Prot: UBE2T_HUMAN, Q9NPD8
    Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
    N-ubiquityllysine
    Enzyme Number (IUBMB): EC 6.3.2.19 

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842 ubiquitin-protein ligase activity IEA--
    GO:0016874 ligase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    UniProtKB/Swiss-Prot: UBE2T_HUMAN, Q9NPD8
    Pathway: Protein modification; protein ubiquitination

     Gene Network CentralTM Interacting Genes and Proteins Network for  UBE2T 


    3 Interacting proteins for UBE2T (ENSP000003562433) via UniProtKB, MINT, and/or STRING

    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBCENSP000003448183STRING (score=.957)
    FANCLENSP000002337413STRING (score=.892)
    FANCD2ENSP000002876473STRING (score=.868)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019941 modification-dependent protein catabolic process IEA--
    GO:0043687 post-translational protein modification IEA--
    GO:0051246 regulation of protein metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBE2T

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2T)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2T)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014176

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2T  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2T  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014176  

    REFSEQ mRNAs for UBE2T gene: 

    NM_014176.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014176  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014176
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014176
                                     untagged cDNA clone in CMV expression vector: NM_014176 

    Additional cDNA sequence: 

    AB032931.1 AF160215.1 AF161499.1 AK000504.1 AY542309.1 BC004152.2 BC019284.1 

    10 DOTS entries:

    DT.448219  DT.95326922  DT.95275330  DT.121353904  DT.95275329  DT.121354001  DT.95219442  DT.95275324 
    DT.91749377  DT.100782806 

    24/167 AceView cDNA sequences (see all 167 ):

    BF032589 BI087825 CA424899 AW376655 BU631864 AI377004 BQ219156 AI791736 
    AI032574 BE778001 AI056896 BQ015257 AA046828 BC019284 CN479657 AI146665 
    BQ430814 AI140513 AF160215 BU634367 AA046871 BC004152 AA705231 AK000504 

    highest scoring ESTs for UBE2T:

    AA263084 AF160215 AF161499 AI791736 AI990409 AK000504 AW250547 BC004152 BC019284 BF032589 

    Unigene Cluster for UBE2T:

    Ubiquitin-conjugating enzyme E2T (putative)
    Hs.5199  [show with all ESTs]
    Unigene Representative Sequence: BF690859


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2T

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7
    SP1:                          -                                       
    SP2:                          -                                       
    SP3:                          -     -                                 
    SP4:                                                                  

    About this scheme

    ECgene alternative splicing isoforms for UBE2T

    1 Ensembl transcript including schematic representation:
    ENST00000367274  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBE2T expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBE2T

    1 / 2 / 3

    3 probe-sets matching UBE2T gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    45803_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI990409 0.80 1.00 0.91 1

    223229_at2, 3 U133-B 1 1.00 1.00 -- -- AB032931 0.60 1.00 0.82 1

    223229_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  UBE2T for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CAAGACAGAA

    SOURCE GeneReport for Unigene cluster: Hs.5199
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBE2T gene from 5/13 species (see all 13 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    UBE2T1   -- ubiquitin-conjugating enzyme E2T (putative) 90.65(n)
    90.31(a)
    607154  XM_843751.1  XP_848844.1 
    chimpanzee
    (Pan troglodytes)
    UBE2T1   -- ubiquitin-conjugating enzyme E2T (putative) 99.83(n)
    99.49(a)
    457628  XM_514102.2  XP_514102.1 
    cow
    (Bos taurus)
    UBE2T1   -- ubiquitin-conjugating enzyme E2T (putative) 80.51(n)
    82.56(a)
    505314  NM_001038034.1  NP_001033123.1 
    rat
    (Rattus norvegicus)
    Ube2t1   -- ubiquitin-conjugating enzyme E2T (putative) 83.84(n)
    82.65(a)
    360847  XM_341124.3  XP_341125.1 
    mouse
    (Mus musculus)
    Ube2t1, 5 15
    ubiquitin-conjugating enzyme E2T (putative)1, 5 84.18(n)1
    85.71(a)1
    671961  NM_026024.21  NP_080300.11 
     AK0125655  AK0212135  (see all 8)
    About this table        Species with no ortholog for UBE2T

    ENSEMBL Gene Tree for UBE2T
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBE2T gene
    UBE2K2  UBE2NL2  UBE2N2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/76 NCBI SNPs in UBE2T are shown (see all 76 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 32)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs127582481,2
    H200578691(+) GTTAAG/ACCAAC 1 -- ng31 tfbs34Minor allele frequency- A:0.00EU EA WA 416
    rs144511,2
    A,C,F,H200571491(-) GCTTCA/GCGTCT 1 S/S syn1 ese319Minor allele frequency- G:0.33NA EA MN EU WA 3160
    rs7888011,2
    A,C,F,H,O200567914(-) TTAAAT/CAGCAG 1 -- int112Minor allele frequency- C:0.28NA EA EU WA 980
    rs104655911,2
    A,C,F,H200574967(+) tacttA/Gaatct 1 -- int14Minor allele frequency- G:0.46EU EA WA 418
    --
    rs7887861,2
    C,F,H200574134(-) tattaG/Cctggg 1 -- int14Minor allele frequency- C:0.03EU EA WA 372
    rs174908641,2
    C,F,H200568855(+) ATGAAA/GTCAAA 1 -- int111Minor allele frequency- G:0.04NA EU EA WA 978
    rs7887891,2
    C,F,H200569613(-) TTTCAT/AAGGGG 1 -- int14Minor allele frequency- A:0.04EU EA WA 418
    --
    rs7887871,2
    A,C200571927(-) ccagcA/Gctttg 1 -- int10--------
    --
    rs75254551,2
    A,C200572876(+) tttagC/Tagaga 1 -- int10--------
    --
    rs7057331,2
    A,C200574032(-) gagcaC/Taccat 1 -- int10--------
    About this table

    HapMap Linkage Disequilibrium images for UBE2T (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610538

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/14 PubMed articles for UBE2T gene (see all 14 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 29089 HGNC: 25009 AceView: HSPC150 Ensembl:ENSG00000077152 euGenes: HUgn29089
    ECgene: UBE2T H-InvDB: UBE2T
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBE2T:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for UBE2T Antibodies for UBE2T
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for UBE2T Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search Tocris compounds for UBE2T




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     Antibodies for UBE2T

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