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UBE2O Gene

protein-coding   GIFtS: 56

GC17M071898
ubiquitin-conjugating enzyme E2O
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
E2-230K 1, 2, 3
EC 6.3.2.19 3
FLJ12878 2
KIAA1734 2, 3
Descriptions
Ubiquitin carrier protein O 3
Ubiquitin-conjugating enzyme E2 of 230 kDa 3
Ubiquitin-protein ligase O 3
ubiquitin-conjugating enzyme E2O 2
External Ids
HGNC: 295541
Entrez Gene: 638932
UniProtKB: Q9C0C93
Ensembl: ENSG000001759317
Search outside databases for aliases for UBE2O gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

Gene Wiki entry for UBE2O

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2O gene  

Entrez Gene cytogenetic band: 17q25.1   Ensembl cytogenetic band:  17q25.1   HGNC cytogenetic band: 17q25.1

UBE2O Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M071898:     (about GC identifiers)

Start:
71,897,491 bp from pter
End:
71,960,883 bp from pter
Size:
63,393 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000017.9  NT_010641.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 O  
Size: 1292 amino acids; 141293 Da
Secondary accessions: A6NDU5 Q69YP4 Q6PIZ2 Q86UA4 Q8N425 Q8TBN1 Q9BSW1 Q9H6E6 Q9H7E4 Q9H9B2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_071349.3  

    ENSEMBL proteins: 
    ENSP00000323687 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (E2 230K)
    Human Recombinant Proteins from Abnova (UBE2O)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for UBE2O: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (E2 230K), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (UBE2O)
    Novus Biologicals Antibodies for UBE2O

    Assays for UBE2O: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugat/RWD-like


       GeneDecks  UBE2O for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9C0C9

    ProtoNet protein and cluster: Q9C0C9

    1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9
    Similarity: Belongs to the ubiquitin-conjugating enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2O)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022066

                  Applied Biosystems Silencer® siRNAs for UBE2O

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2O  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2O  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBE2O
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_022066
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_022066
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_022066 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_022066

    UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9
    Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
    N-ubiquityllysine
    Enzyme Number (IUBMB): EC 6.3.2.19 

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842 ubiquitin-protein ligase activity IEA--
    GO:0016874 ligase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for UBE2O):
     hsa04120 Ubiquitin mediated proteolysis

       GeneDecks  UBE2O for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9
    Pathway: Protein modification; protein ubiquitination


    1 Interacting protein for UBE2O (ENSP000003236873) via UniProtKB, MINT, and/or STRING

    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBE2HENSP000003478363STRING (score=.732)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019941 modification-dependent protein catabolic process IEA--
    GO:0043687 post-translational protein modification IEA--
    GO:0051246 regulation of protein metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBE2O

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2O)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022066

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2O  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2O  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_022066  

    REFSEQ mRNAs for UBE2O gene: 

    NM_022066.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_022066  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_022066
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_022066
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_022066 

    Additional cDNA sequence: 

    AB051521.1 AK000926.1 AK022940.1 AK024657.1 AK025999.1 AK225412.1 AL832432.1 BC004525.2 
    BC022237.1 BC025977.1 BC036820.1 BC051868.2 

    7 DOTS entries:

    DT.415431  DT.100780454  DT.95295490  DT.92454174  DT.99932745  DT.101975536  DT.120980180 

    24/233 AceView cDNA sequences (see all 233 ):

    AK022940 BQ070316 BU740780 BM727219 AW152157 CD672580 AI160123 BQ645258 
    BP371308 BU189342 BC004525 AI433415 BU948231 BF593833 AI538409 BQ217875 
    BE645208 AI698890 AI831836 BQ071266 BQ437129 AK025999 NM_022066 BU859314 

    highest scoring ESTs for UBE2O:

    AA195077 AA341325 AB051521 BC007822 BF726224 CB960973 CD580295 CK000406 AA135179 AA135353 

    Unigene Cluster for UBE2O:

    Ubiquitin-conjugating enzyme E2O
    Hs.16130  [show with all ESTs]
    Unigene Representative Sequence: NM_022066


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2O

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18
    SP1:                                      -                                                                       -                     
    SP2:                                      -                                                                                             
    SP3:                                                                                                              -                     
    SP4:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for UBE2O

    1 Ensembl transcript including schematic representation:
    ENST00000319380  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBE2O expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBE2O

    1 / 2 / 3

    4 probe-sets matching UBE2O gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    54633_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA195077 0.80 1.00 0.91 1

    218141_at2, 3 U133-A 1 1.00 1.00 -- -- NM_022066 0.60 1.00 0.82 1

    218141_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1554814_at*2 U133Plus2 NULL 0.00 0.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGGAGATGTG

    SOURCE GeneReport for Unigene cluster: Hs.16130

    UniProtKB/Swiss-Prot: UBE2O_HUMAN, Q9C0C9
    Tissue specificity: Predominantly expressed in skeletal muscle and heart

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBE2O gene from 5/13 species (see all 13 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    UBE2O1   -- ubiquitin-conjugating enzyme E2O 90.48(n)
    94.87(a)
    483330  XM_540449.2  XP_540449.2 
    chimpanzee
    (Pan troglodytes)
    UBE2O1   -- ubiquitin-conjugating enzyme E2O 99.35(n)
    99.82(a)
    468335  XM_001139963.1  XP_001139963.1 
    cow
    (Bos taurus)
    UBE2O1   -- ubiquitin-conjugating enzyme E2O 89.72(n)
    94.99(a)
    789422  XM_001256183.1  XP_001256184.1 
    rat
    (Rattus norvegicus)
    Ube2o1   -- ubiquitin-conjugating enzyme E2O 89.02(n)
    94.64(a)
    303689  XM_221132.4  XP_221132.4 
    mouse
    (Mus musculus)
    Ube2o1, 5 115
    ubiquitin-conjugating enzyme E2O1, 5 88.36(n)1
    94.09(a)1
    2173421  NM_173755.31  NP_776116.21 
     AI8535325  AK0359705  (see all 18)
    About this table        Species with no ortholog for UBE2O

    ENSEMBL Gene Tree for UBE2O
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBE2O gene
    UBE2Z2  BIRC62  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/352 NCBI SNPs in UBE2O are shown (see all 352 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 110)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs38037391,2
    A,C,F,H71898879(+) CAGGCC/TCTGGG 1 G/S mis1 ese38Minor allele frequency- T:0.33EA MN EU WA NA 3218
    --
    rs80727431,2
    A,C71962149(+) agagcA/Gagact 1 -- ng310--------
    --
    rs80767321,2
    A,C71962219(+) aggcgC/Tggtgg 1 -- ng310--------
    --
    rs72105201,2
    A,C71961635(+) gcacaC/Ttgatt 1 -- ng310--------
    --
    rs8980841,2
    C71897186(-) TTTTTG/TTGTTG 1 -- ng510--------
    rs37440431,2
    C,F,H71906529(+) ACCACG/AAAGTC 1 F/F syn18Minor allele frequency- A:0.13EA NA EU WA 2048
    rs4528681,2
    A,C,F,H,O71933078(+) aaaaaA/Caggca 1 -- int19Minor allele frequency- C:0.14EU EA WA MN 1022
    rs3898091,2
    A,C,F,H,O71925592(-) TACCAA/GCGGAA 1 -- int112Minor allele frequency- G:0.04MN NA EU EA WA 1164
    rs4479211,2
    A,C,F,H,O71926154(-) ATCTAG/AACTTC 1 -- int17Minor allele frequency- A:0.12EU EA WA NA 562
    rs3468161,2
    A,C,F,H71902533(-) TATTAT/CGATTA 1 -- int17Minor allele frequency- C:0.09EU EA WA NA 534
    About this table

    HapMap Linkage Disequilibrium images for UBE2O (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for UBE2O gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 63893 HGNC: 29554 AceView: E2-230K Ensembl:ENSG00000175931 euGenes: HUgn63893
    ECgene: UBE2O H-InvDB: UBE2O
    (According to HUGE)
    About This Section

    HUGE: KIAA1734
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBE2O:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Predesigned and custom siRNAs for UBE2O Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for UBE2O Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (E2 230K)
     Antibodies (E2 230K)
     Search OriGene for UBE2O
     Search Tocris compounds for UBE2O




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     Antibodies for UBE2O

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