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Aliases for UBE2N Gene

Aliases for UBE2N Gene

  • Ubiquitin Conjugating Enzyme E2 N 2 3
  • Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13) 2 3
  • Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast) 2 3
  • Bendless-Like Ubiquitin-Conjugating Enzyme 3 4
  • E2 Ubiquitin-Conjugating Enzyme N 3 4
  • Ubiquitin Conjugating Enzyme E2N 3 5
  • Ubiquitin Carrier Protein N 3 4
  • Ubiquitin-Protein Ligase N 3 4
  • UbcH13 3 4
  • UBC13 3 4
  • Bendless-Like Ubiquitin Conjugating Enzyme 3
  • Epididymis Secretory Protein Li 71 3
  • Ubiquitin-Conjugating Enzyme E2N 2
  • Yeast UBC13 Homolog 3
  • UBCHBEN; UBC13 3
  • EC 6.3.2.19 63
  • EC 2.3.2.23 4
  • HEL-S-71 3
  • UbcH-Ben 3
  • BLU 4

External Ids for UBE2N Gene

Previous GeneCards Identifiers for UBE2N Gene

  • GC12M094324
  • GC12M093735
  • GC12M092305
  • GC12M092304
  • GC12M090868

Summaries for UBE2N Gene

Entrez Gene Summary for UBE2N Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2N Gene

UBE2N (Ubiquitin Conjugating Enzyme E2 N) is a Protein Coding gene. Diseases associated with UBE2N include Ciliary Dyskinesia, Primary, 22 and Riddle Syndrome. Among its related pathways are Cell Cycle Checkpoints and TCR signaling (REACTOME). GO annotations related to this gene include poly(A) RNA binding and ubiquitin-protein transferase activity. An important paralog of this gene is UBE2NL.

UniProtKB/Swiss-Prot for UBE2N Gene

  • The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the Lys-63-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the Lys-63-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate Lys-63-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

Tocris Summary for UBE2N Gene

  • The ubiquitin (Ub)/Ub-like (Ubl) conjugating enzymes are a conserved family of ligases which mediate the conjugation of Ub and Ubl proteins to target substrates. There have been 35 E2 enzymes identified in the human genome to date.

Gene Wiki entry for UBE2N Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for UBE2N Gene

Genomics for UBE2N Gene

Regulatory Elements for UBE2N Gene

Enhancers for UBE2N Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around UBE2N on UCSC Golden Path with GeneCards custom track

Promoters for UBE2N Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around UBE2N on UCSC Golden Path with GeneCards custom track

Genomic Location for UBE2N Gene

Chromosome:
12
Start:
93,405,673 bp from pter
End:
93,443,445 bp from pter
Size:
37,773 bases
Orientation:
Minus strand

Genomic View for UBE2N Gene

Genes around UBE2N on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
UBE2N Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for UBE2N Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2N Gene

Proteins for UBE2N Gene

  • Protein details for UBE2N Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61088-UBE2N_HUMAN
    Recommended name:
    Ubiquitin-conjugating enzyme E2 N
    Protein Accession:
    P61088
    Secondary Accessions:
    • Q16781
    • Q53Y81

    Protein attributes for UBE2N Gene

    Size:
    152 amino acids
    Molecular mass:
    17138 Da
    Quaternary structure:
    • Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific Lys-63-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the Lys-63-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Interacts with OTUB1; leading to inhibit E2-conjugating activity.

    Three dimensional structures from OCA and Proteopedia for UBE2N Gene

neXtProt entry for UBE2N Gene

Proteomics data for UBE2N Gene at MOPED

Selected DME Specific Peptides for UBE2N Gene

Post-translational modifications for UBE2N Gene

  • Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Ubiquitination at Lys 68, Lys 82, and Lys 94
  • Modification sites at PhosphoSitePlus

Other Protein References for UBE2N Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for UBE2N (UBE2N)

Domains & Families for UBE2N Gene

Gene Families for UBE2N Gene

Protein Domains for UBE2N Gene

Suggested Antigen Peptide Sequences for UBE2N Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61088

UniProtKB/Swiss-Prot:

UBE2N_HUMAN :
  • Belongs to the ubiquitin-conjugating enzyme family.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family.
genes like me logo Genes that share domains with UBE2N: view

Function for UBE2N Gene

Molecular function for UBE2N Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activity is inhibited by binding to OTUB1, which prevents Lys-63-linked polyubiquitination.
UniProtKB/Swiss-Prot Function:
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the Lys-63-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the Lys-63-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate Lys-63-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

Enzyme Numbers (IUBMB) for UBE2N Gene

Gene Ontology (GO) - Molecular Function for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0061630 ubiquitin protein ligase activity IBA --
genes like me logo Genes that share ontologies with UBE2N: view
genes like me logo Genes that share phenotypes with UBE2N: view

Animal Models for UBE2N Gene

MGI Knock Outs for UBE2N:

Animal Model Products

  • Taconic Biosciences Mouse Models for UBE2N

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for UBE2N Gene

Localization for UBE2N Gene

Subcellular locations from UniProtKB/Swiss-Prot for UBE2N Gene

Nucleus. Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for UBE2N Gene COMPARTMENTS Subcellular localization image for UBE2N gene
Compartment Confidence
cytosol 5
extracellular 5
nucleus 5
mitochondrion 2
lysosome 1
peroxisome 1
vacuole 1

Gene Ontology (GO) - Cellular Components for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0035370 UBC13-UEV1A complex IDA 16307917
GO:0070062 extracellular exosome IDA 20458337
genes like me logo Genes that share ontologies with UBE2N: view

Pathways & Interactions for UBE2N Gene

SuperPathways for UBE2N Gene

Superpath Contained pathways
1 MyD88 cascade initiated on plasma membrane
2 DNA Double-Strand Break Repair
3 Antigen processing- Ubiquitination and Proteasome degradation
4 Immune System
5 Interleukin-3, 5 and GM-CSF signaling
genes like me logo Genes that share pathways with UBE2N: view

UniProtKB/Swiss-Prot P61088-UBE2N_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for UBE2N Gene

Activates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000729 DNA double-strand break processing IMP 17349954
GO:0002755 MyD88-dependent toll-like receptor signaling pathway TAS --
GO:0006281 DNA repair TAS --
GO:0006289 nucleotide-excision repair TAS --
GO:0006303 double-strand break repair via nonhomologous end joining TAS --
genes like me logo Genes that share ontologies with UBE2N: view

Drugs & Compounds for UBE2N Gene

(4) Drugs for UBE2N Gene - From: ApexBio, HMDB, and Tocris

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine monophosphate Approved Nutra 0
Adenosine triphosphate Approved Nutra 0
Diphosphate Experimental Pharma 0
NSC697923 Pharma Inhibitor of E2 complex Ubc13-Uev1A,cell permeable and selective, Selective UBE2N inhibitor 0

(2) Additional Compounds for UBE2N Gene - From: Tocris and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
phosphoric acid
  • Acide phosphorique (FRENCH)
  • Acido fosforico [Italian]
  • Acidum phosphoricum
  • Diphosphate tetrasodium
  • Fosforzuuroplossingen [Dutch]
7664-38-2
Bay 11-7821
19542-67-7

(2) Tocris Compounds for UBE2N Gene

Compound Action Cas Number
Bay 11-7821 E2 ubiquitin (Ub) conjugating enzymes inhibitor 19542-67-7
NSC 697923 Selective UBE2N inhibitor 343351-67-7

(1) ApexBio Compounds for UBE2N Gene

Compound Action Cas Number
NSC697923 Inhibitor of E2 complex Ubc13-Uev1A,cell permeable and selective 343351-67-7
genes like me logo Genes that share compounds with UBE2N: view

Drug Products

Transcripts for UBE2N Gene

Unigene Clusters for UBE2N Gene

Ubiquitin-conjugating enzyme E2N:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2N Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c · 7d
SP1: -
SP2: - - -
SP3: - - - -
SP4: - -
SP5: - - -
SP6: - -

Relevant External Links for UBE2N Gene

GeneLoc Exon Structure for
UBE2N
ECgene alternative splicing isoforms for
UBE2N

Expression for UBE2N Gene

mRNA expression in normal human tissues for UBE2N Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for UBE2N Gene



SOURCE GeneReport for Unigene cluster for UBE2N Gene Hs.524630

genes like me logo Genes that share expression patterns with UBE2N: view

Protein tissue co-expression partners for UBE2N Gene

- Elite partner

Primer Products

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for UBE2N Gene

Orthologs for UBE2N Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2N Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia UBE2N 35
  • 98.25 (n)
  • 100 (a)
UBE2N 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia UBE2N 35
  • 98.46 (n)
  • 100 (a)
UBE2N 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ube2n 35
  • 97.59 (n)
  • 100 (a)
Ube2n 16
Ube2n 36
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia UBE2N 35
  • 100 (n)
  • 100 (a)
UBE2N 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ube2n 35
  • 97.15 (n)
  • 99.34 (a)
oppossum
(Monodelphis domestica)
Mammalia UBE2N 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia UBE2N 36
  • 95 (a)
OneToOne
chicken
(Gallus gallus)
Aves UBE2N 35
  • 92.54 (n)
  • 99.34 (a)
UBE2N 36
  • 99 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2N 36
  • 100 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia MGC75672 35
ube2n 35
  • 89.04 (n)
  • 98.03 (a)
African clawed frog
(Xenopus laevis)
Amphibia ube2n-prov 35
zebrafish
(Danio rerio)
Actinopterygii Dr.5512 35
ube2na 35
  • 85.53 (n)
  • 98.03 (a)
ube2na 36
  • 94 (a)
ManyToMany
ube2nb 36
  • 94 (a)
ManyToMany
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8375 35
fruit fly
(Drosophila melanogaster)
Insecta ben 37
  • 80 (a)
BG:DS01486.1 37
  • 83 (a)
CG3473 35
  • 70.64 (n)
  • 78.81 (a)
worm
(Caenorhabditis elegans)
Secernentea ubc-13 35
  • 70.95 (n)
  • 89.19 (a)
ubc-13 36
  • 85 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC13 36
  • 66 (a)
OneToMany
UBC13 38
thale cress
(Arabidopsis thaliana)
eudicotyledons UBC36 35
  • 66.44 (n)
  • 80.27 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.508 35
rice
(Oryza sativa)
Liliopsida Os.14354 35
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3622 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 71 (a)
OneToMany
Species with no ortholog for UBE2N:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for UBE2N Gene

ENSEMBL:
Gene Tree for UBE2N (if available)
TreeFam:
Gene Tree for UBE2N (if available)

Paralogs for UBE2N Gene

Paralogs for UBE2N Gene

Pseudogenes.org Pseudogenes for UBE2N Gene

genes like me logo Genes that share paralogs with UBE2N: view

Variants for UBE2N Gene

Sequence variations from dbSNP and Humsavar for UBE2N Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs7295360 -- 93,436,187(+) gcctc(A/G)acttc intron-variant
rs7295796 -- 93,436,285(+) ttttt(C/G)tagag intron-variant
rs471862 -- 93,425,978(+) TCCCA(A/C/G/T)AACCT intron-variant
rs7299705 -- 93,437,269(+) tagga(A/C)ttcga intron-variant
rs486398 -- 93,423,173(+) TGAGC(A/G)CTGAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for UBE2N Gene

Variant ID Type Subtype PubMed ID
nsv7234 OTHER Inversion 18451855
esv272008 CNV Insertion 20981092
nsv823 CNV Loss 18451855
nsv528151 CNV Loss 19592680

Variation tolerance for UBE2N Gene

Residual Variation Intolerance Score: 50.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.05; 1.04% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for UBE2N Gene

Disorders for UBE2N Gene

MalaCards: The human disease database

(2) MalaCards diseases for UBE2N Gene - From: OMIM and GeneCards

Disorder Aliases PubMed IDs
ciliary dyskinesia, primary, 22
  • primary ciliary dyskinesia 22 with or without situs inversus
riddle syndrome
  • radiosensitivity, immunodeficiency, dysmorphic features, and learning difficulties
- elite association - COSMIC cancer census association via MalaCards
Search UBE2N in MalaCards View complete list of genes associated with diseases

Relevant External Links for UBE2N

Genetic Association Database (GAD)
UBE2N
Human Genome Epidemiology (HuGE) Navigator
UBE2N
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
UBE2N
genes like me logo Genes that share disorders with UBE2N: view

No data available for UniProtKB/Swiss-Prot and Genatlas for UBE2N Gene

Publications for UBE2N Gene

  1. Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product. (PMID: 8902611) Yamaguchi T. … Kato S. (J. Biochem. 1996) 2 3 4 67
  2. Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes. (PMID: 20146531) Graf C. … Mayer M.P. (Biochemistry 2010) 3 23
  3. Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. (PMID: 18615712) Scheper J. … Thomson T.M. (Proteins 2009) 3 23
  4. The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains. (PMID: 19340006) Marteijn J.A. … van der Reijden B.A. (Leukemia 2009) 3 23
  5. E2 interaction and dimerization in the crystal structure of TRAF6. (PMID: 19465916) Yin Q. … Wu H. (Nat. Struct. Mol. Biol. 2009) 3 23

Products for UBE2N Gene

Sources for UBE2N Gene

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