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UBE2N Gene

protein-coding   GIFtS: 69
GCID: GC12M093735

Ubiquitin-Conjugating Enzyme E2N

(Previous names: ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13),...)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ubiquitin-Conjugating Enzyme E2N1 2     UBC132
Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13)1 2     UbcH-ben2
Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast)1 2     Bendless-Like Ubiquitin Conjugating Enzyme2
Bendless-Like Ubiquitin-Conjugating Enzyme2 3     Epididymis Secretory Protein Li 712
Ubiquitin Carrier Protein N2 3     Ubiquitin-Conjugating Enzyme E2 N2
Ubiquitin-Protein Ligase N2 3     Yeast UBC13 Homolog2
UbcH132 3     BLU3
EC 6.3.2.193 8     Ubc133
HEL-S-712     

External Ids:    HGNC: 124921   Entrez Gene: 73342   Ensembl: ENSG000001778897   OMIM: 6036795   UniProtKB: P610883   

Export aliases for UBE2N gene to outside databases

Previous GC identifers: GC12M093423 GC12M094324 GC12M092305 GC12M092304 GC12M090868


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for UBE2N Gene:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or
short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes:
ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or
E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this
protein plays a role in DNA postreplication repair. (provided by RefSeq, Jul 2008)

GeneCards Summary for UBE2N Gene:
UBE2N (ubiquitin-conjugating enzyme E2N) is a protein-coding gene. Diseases associated with UBE2N include riddle syndrome, and machado-joseph disease. GO annotations related to this gene include ubiquitin binding and ubiquitin-protein ligase activity. An important paralog of this gene is UBE2T.

UniProtKB/Swiss-Prot: UBE2N_HUMAN, P61088
Function: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked
polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome.
Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell
cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of
cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked
poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with
E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing
its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity
and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains
which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and
MAPK-responsive inflammatory genes (By similarity)

Gene Wiki entry for UBE2N Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000012.12  NC_018923.2  NT_029419.13  
Regulatory elements:
   Regulatory transcription factor binding sites in the UBE2N gene promoter:
         HOXA9   HOXA9B   Sp1   Egr-1   XBP-1   Meis-1b   Evi-1   Meis-1a   ATF   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidUBE2N promoter sequence
   Search Chromatin IP Primers for UBE2N

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat UBE2N


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q22   Ensembl cytogenetic band:  12q22   HGNC cytogenetic band: 12q22

UBE2N Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
UBE2N gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M093735:  view genomic region     (about GC identifiers)

Start:
93,799,449 bp from pter      End:
93,836,038 bp from pter
Size:
36,590 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: UBE2N_HUMAN, P61088 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 N  
Size: 152 amino acids; 17138 Da
Subunit: Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box
domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168.
Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked
polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2).
Interacts with OTUB1; leading to inhibit E2-conjugating activity
Selected PDB 3D structures from and Proteopedia for UBE2N (see all 11):
1J7D (3D)        2C2V (3D)        3HCT (3D)        3HCU (3D)        3VON (3D)        3W31 (3D)    
Secondary accessions: Q16781 Q53Y81

Explore the universe of human proteins at neXtProt for UBE2N: NX_P61088

Explore proteomics data for UBE2N at MOPED

Post-translational modifications: 

  • Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V21
  • Ubiquitination2 at Lys10, Lys24, Lys68, Lys74, Lys82, Lys92, Lys94
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for UBE2N (P61088) (see all 6)
     ICLDILK  TKIYHPN  FKLELFLPE  SIQALLSAP 


    See UBE2N Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_003339.1  
    ENSEMBL proteins: 
     ENSP00000316176   ENSP00000449352   ENSP00000450260   ENSP00000447009   ENSP00000448352  
    Reactome Protein details: P61088

    UBE2N Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for UBE2N (UBE2N/Ubc13, UBE2N (Ubc13)/Uev1a Complex)
    Enzo Life Sciences proteins for UBE2N
    OriGene Purified Protein for UBE2N
    OriGene Protein Over-expression Lysate for UBE2N
    OriGene MassSpec for UBE2N
    OriGene Custom Protein Services for UBE2N
    GenScript Custom Purified and Recombinant Proteins Services for UBE2N
    Novus Biologicals UBE2N Proteins
    Novus Biologicals UBE2N Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for UBE2N
    Cloud-Clone Corp. Proteins for UBE2N

    UBE2N Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of UBE2N
    R&D Systems Antibodies for UBE2N (UBE2N/Ubc13)
    Cell Signaling Technology (CST) Antibodies for UBE2N 
    OriGene Antibodies for UBE2N
    OriGene Custom Antibody Services for UBE2N
    Novus Biologicals UBE2N Antibodies
    Search for Antibodies for UBE2N at Abcam
    Cloud-Clone Corp. Antibodies for UBE2N
    ThermoFisher Antibodies for UBE2N
    LSBio Antibodies in human, mouse, rat for UBE2N

    UBE2N Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for UBE2N
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for UBE2N
    Cloud-Clone Corp. CLIAs for UBE2N


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    UBE2: Ubiquitin-conjugating enzymes E2

    3 InterPro protein domains:
     IPR023313 UBQ-conjugating_AS
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugating_enzyme/RWD

    Graphical View of Domain Structure for InterPro Entry P61088

    ProtoNet protein and cluster: P61088

    1 Blocks protein domain: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UBE2N_HUMAN, P61088
    Similarity: Belongs to the ubiquitin-conjugating enzyme family


    UBE2N for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: UBE2N_HUMAN, P61088
    Function: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked
    polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome.
    Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell
    cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of
    cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked
    poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with
    E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing
    its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity
    and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains
    which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and
    MAPK-responsive inflammatory genes (By similarity)
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
    Enzyme regulation: Activity is inhibited by binding to OTUB1, which prevents 'Lys-63'-linked polyubiquitination

         Enzyme Number (IUBMB): EC 6.3.2.191 2

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity IDA--
    GO:0005515protein binding IPI11473255
    GO:0005524ATP binding IEA--
    GO:0016881acid-amino acid ligase activity ----
    GO:0043130ubiquitin binding IDA16122702
         
    UBE2N for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for UBE2N:
     Increased G2M DNA content  Increased cell death HMECs cel  Increased number of mitotic ce 

         5 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Ube2n):
     cellular  hematopoietic system  immune system  mortality/aging  normal 

    UBE2N for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Ube2ntm2Aki for UBE2N

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for UBE2N
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for UBE2N

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for UBE2N
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for UBE2N

    miRNA
    Products:
        
    miRTarBase miRNAs that target UBE2N:
    hsa-mir-128-3p (MIRT021990), hsa-mir-877-5p (MIRT037291), hsa-mir-96-5p (MIRT027911), hsa-mir-744-5p (MIRT037679), hsa-mir-149-5p (MIRT045605), hsa-mir-221-3p (MIRT024180)

    Block miRNA regulation of human, mouse, rat UBE2N using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate UBE2N (see all 48):
    hsa-miR-549 hsa-miR-100* hsa-miR-513a-5p hsa-miR-128 hsa-miR-219-5p hsa-miR-1184 hsa-miR-3613-3p hsa-miR-34b
    SwitchGear 3'UTR luciferase reporter plasmidUBE2N 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for UBE2N
    Predesigned siRNA for gene silencing in human, mouse, rat UBE2N

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for UBE2N

    Clone
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    OriGene clones in human, mouse for UBE2N (see all 8)
    OriGene ORF clones in mouse, rat for UBE2N
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: UBE2N (NM_003348)
    Sino Biological Human cDNA Clone for UBE2N
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for UBE2N
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat UBE2N

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for UBE2N 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for UBE2N


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    UBE2N_HUMAN, P61088: Nucleus. Cytoplasm
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol4
    mitochondrion2
    peroxisome1

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000151ubiquitin ligase complex IDA16307917
    GO:0005634nucleus IDA16129784
    GO:0005737cytoplasm TAS16129784
    GO:0005829cytosol TAS--
    GO:0031372UBC13-MMS2 complex IDA16129784

    UBE2N for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for UBE2N About   (see all 19)  
    See pathways by source

    SuperPathContained pathways About
    1Class I MHC mediated antigen processing and presentation
    Class I MHC mediated antigen processing and presentation0.84
    Adaptive Immune System0.41
    Antigen processing- Ubiquitination and Proteasome degradation0.84
    Ubiquitin mediated proteolysis0.35
    2Interleukin-1 signaling
    Interleukin-1 signaling0.45
    IL1-mediated signaling events0.45
    3Toll Like Receptor 7/8 (TLR7/8) Cascade
    TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation0.93
    MyD88-independent cascade0.69
    Toll Like Receptor 9 (TLR9) Cascade0.93
    Toll Like Receptor 3 (TLR3) Cascade0.69
    Toll Like Receptor 7/8 (TLR7/8) Cascade0.69
    TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling0.00
    MyD88 dependent cascade initiated on endosome0.69
    4Interferon Signaling
    Interferon Signaling0.59
    ISG15 antiviral mechanism0.42
    Cytokine Signaling in Immune system0.59
    Antiviral mechanism by IFN-stimulated genes0.42
    5TCR signaling (REACTOME)
    Downstream TCR signaling0.73
    TCR signaling0.73

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for UBE2N
        Ubiquitin-Proteasome Dependent Proteolysis
    Remodeling of Adherens Junctions
    Toll-Like Receptors Pathway

    3 Cell Signaling Technology (CST) Pathways for UBE2N
        DNA Damage
    NF-kappaB Signaling
    Protein Stability

    3 GeneGo (Thomson Reuters) Pathways for UBE2N
        Immune response T cell receptor signaling pathway
    Proteolysis Putative ubiquitin pathway
    Immune response IL-1 signaling pathway

    1 BioSystems Pathway for UBE2N
        IL1-mediated signaling events


    Selected Reactome Pathways for UBE2N (see all 10)
        IRAK1 recruits IKK complex
    IKK complex recruitment mediated by RIP1
    FCERI mediated NF-kB activation
    TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling
    Interleukin-1 signaling


    1 Kegg Pathway  (Kegg details for UBE2N):
        Ubiquitin mediated proteolysis

    UniProtKB/Swiss-Prot: UBE2N_HUMAN, P61088
    Pathway: Protein modification; protein ubiquitination


    UBE2N for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including UBE2N: 

              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Toll-Like Receptor Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for UBE2N

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for UBE2N (P610881, 2, 3 ENSP000003161764) via UniProtKB, MINT, STRING, and/or I2D (see all 994)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HLTFQ145272, 3, ENSP000003089444MINT-7945693 MINT-7947479 I2D: score=2 STRING: ENSP00000308944
    RNF168Q8IYW51, 3, ENSP000003208984EBI-1052908,EBI-914207 I2D: score=2 STRING: ENSP00000320898
    HSPA8P111422, 3MINT-7945693 MINT-7947479 I2D: score=1 
    POLR1CO151602, 3MINT-7945693 MINT-7947479 I2D: score=1 
    RNF130Q86XS82, 3, ENSP000002619474MINT-7947479 I2D: score=1 STRING: ENSP00000261947
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 31):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000724double-strand break repair via homologous recombination IMP17349954
    GO:0000729DNA double-strand break processing IMP17349954
    GO:0002224toll-like receptor signaling pathway TAS--
    GO:0002755MyD88-dependent toll-like receptor signaling pathway TAS--
    GO:0006282regulation of DNA repair TAS10089880

    UBE2N for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for UBE2N

    4 HMDB Compounds for UBE2N    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for UBE2N gene: 
    NM_003348.3  

    Unigene Cluster for UBE2N:

    Ubiquitin-conjugating enzyme E2N
    Hs.524630  [show with all ESTs]
    Unigene Representative Sequence: NM_003348
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000548946 ENST00000318066(uc001tcp.3) ENST00000550657 ENST00000549833
    ENST00000549490 ENST00000552442
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate UBE2N (see all 48):
    hsa-miR-549 hsa-miR-100* hsa-miR-513a-5p hsa-miR-128 hsa-miR-219-5p hsa-miR-1184 hsa-miR-3613-3p hsa-miR-34b
    SwitchGear 3'UTR luciferase reporter plasmidUBE2N 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat UBE2N
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: UBE2N (NM_003348)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for UBE2N
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat UBE2N
    Primer
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    OriGene qPCR primer pairs and template standards for UBE2N
    OriGene qSTAR qPCR primer pairs in human, mouse for UBE2N
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat UBE2N
      QuantiTect SYBR Green Assays in human, mouse, rat UBE2N
      QuantiFast Probe-based Assays in human, mouse, rat UBE2N

    Additional mRNA sequence: 

    AL110140.1 BC000396.2 BC003365.1 BC108704.1 BT006873.1 D83004.1 

    Selected DOTS entries (see all 25):

    DT.453669  DT.100855012  DT.95269516  DT.100668076  DT.75194839  DT.100853696  DT.121176170  DT.100839675 
    DT.95269519  DT.100668079  DT.100668083  DT.100668084  DT.100668086  DT.100767810  DT.92453396  DT.95118319 
    DT.95219528  DT.95296694  DT.100870368  DT.121176146  DT.121176159  DT.121176172  DT.121176181  DT.121304279 

    Selected AceView cDNA sequences (see all 546):

    AW613028 T16630 BC003365 BQ008224 BU784291 AI338630 BU730096 CD107907 
    AU077052 CB044509 AA910925 BM916123 BQ220048 AI674573 BM846510 BF062491 
    AI360468 BQ434581 BM987307 BE276193 AI078423 BM670237 BQ011070 BQ774918 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for UBE2N (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c · 7d
    SP1:                                -                                                   
    SP2:                    -     -     -                                                   
    SP3:                    -     -     -           -                                       
    SP4:                    -     -                                                         
    SP5:                    -     -     -                                                   


    ECgene alternative splicing isoforms for UBE2N

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    UBE2N expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    UBE2N Expression
    About this image


    UBE2N expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Testis (Reproductive System)
             XY Germ Cells Testis Cord
     
     Gonad
             XY Germ Cells Testis Cord
     
     Brain (Nervous System)
             Cerebral Cortex
     
     Colon (Gastrointestinal Tract)
     
     Prostate (Endocrine System)
    UBE2N Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    UBE2N Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.524630
        Pathway & Disease-focused RT2 Profiler PCR Arrays including UBE2N: 
              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Toll-Like Receptor Signaling Pathway in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for UBE2N gene from Selected species (see all 25)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ube2n1 , 5 ubiquitin-conjugating enzyme E2N1, 5 97.59(n)1
    100(a)1
      10 (49.41 cM)5
    937651  NM_080560.31  NP_542127.11 
     955151625 
    chicken
    (Gallus gallus)
    Aves UBE2N1 ubiquitin-conjugating enzyme E2N 92.54(n)
    99.34(a)
      417898  NM_001012810.1  NP_001012828.1 
    lizard
    (Anolis carolinensis)
    Reptilia UBE2N6
    ubiquitin-conjugating enzyme E2N
    100(a)
    1 ↔ 1
    5(31453665-31467699)
    African clawed frog
    (Xenopus laevis)
    Amphibia ube2n-prov2 ubiquitin-conjugating enzyme E2N 86.38(n)    BC053797.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.55122 Danio rerio mRNA similar to ubiquitin-conjugating enzyme more 85.68(n)    BC044461.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta BG:DS01486.13
    CG34731
    ubiquitin cycle ubiquitin conjugating
    enzyme3
    CG34731
    83(a)
    (best of 2)3
    70.64(n)1
    78.81(a)1
      35B33
    348491  NM_135871.21  NP_609715.11 
    worm
    (Caenorhabditis elegans)
    Secernentea ubc-131 ubc-13 70.95(n)
    89.19(a)
      177073  NM_067871.6  NP_500272.2 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes UBC13(YDR092W)4 Ubiquitin-conjugating enzyme involved in the error-free more   --   4(629876-630605) 851666  NP_010377.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons UBC361 UBC36 66.44(n)
    80.27(a)
      838260  NM_001198088.1  NP_001185017.1 
    rice
    (Oryza sativa)
    Liliopsida Os.143542 Oryza sativa (japonica cultivar-group) ubiquitin-conjugating more 74.09(n)    NM_191984.1 


    ENSEMBL Gene Tree for UBE2N (if available)
    TreeFam Gene Tree for UBE2N (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for UBE2N gene
    UBE2T2  UBE2NL2  
    Selected SIMAP similar genes for UBE2N using alignment to 5 protein entries:     UBE2N_HUMAN (see all proteins) (see all similar genes):
    UBE2NL    UBE2H    HIP2    UBE2D2    UBE2A    UBE2T
    UBE2D3    HR6A    UBE2F    UBE2M    UBE2D4    UBE2B
    UBE2D1    UBE2E2    UBE2L3    UBE2L6    UBE2G1    UBE2I

    UBE2N for paralogs           About GeneDecksing


    4 Pseudogenes.org Pseudogenes for UBE2N
    PGOHUM00000247738 PGOHUM00000240575 PGOHUM00000245771 PGOHUM00000261514


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for UBE2N (see all 740)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1415868171,2
    C--93801681(+) TCTTAA/CCAAAA 1 -- ds50010--------
    rs1882919311,2
    --93801846(+) TTGTAA/CATCAC 1 -- ds50010--------
    rs1508150571,2
    --93801893(+) GGCAGA/TAAATG 1 -- ds50010--------
    rs1395251681,2
    --93801912(+) TATTTC/TGTTTC 1 -- ds50010--------
    rs128219331,2
    H--93801949(+) AAGTGA/TGATTC 1 -- ds50014Minor allele frequency- T:0.00NS EA 414
    rs1442418611,2
    --93801968(+) TAGCTA/GTAGTC 1 -- ds50010--------
    rs128219571,2
    H--93801997(+) GCCCAA/TGAATT 1 -- ds50014Minor allele frequency- T:0.00NS EA 420
    rs128224591,2
    H--93801998(+) CCCAAG/AAATTG 1 -- ds50014Minor allele frequency- A:0.00NS EA 418
    rs1465908861,2
    C--93802100(+) GCAATC/GCATTT 1 -- ut310--------
    rs111070161,2
    H--93802343(+) ACTGGC/TTACAT 1 -- ut31 ese34Minor allele frequency- T:0.00NS EA 420

    HapMap Linkage Disequilibrium report for UBE2N (93799449 - 93836038 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for UBE2N:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv272008CNV Insertion20981092
    nsv528151CNV Loss19592680
    nsv823CNV Loss18451855
    nsv7234OTHER Inversion18451855

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing UBE2N
    DNA2.0 Custom Variant and Variant Library Synthesis for UBE2N

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 603679    OMIM disorders: --

    17 diseases for UBE2N:    
    About MalaCards
    riddle syndrome    machado-joseph disease    leukemia/lymphoma, t-cell    limb-girdle muscular dystrophy
    pregnancy loss    human t-cell leukemia virus type 1    muscular dystrophy    t-cell leukemia
    chronic lymphocytic leukemia    parkinson's disease    hypoxia    schizophrenia
    leukemia    hiv-1    endotheliitis    breast cancer
    malaria


    UBE2N for disorders           About GeneDecksing

    Genetic Association Database (GAD): UBE2N
    Human Genome Epidemiology (HuGE) Navigator: UBE2N (2 documents)

    Export disorders for UBE2N gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for UBE2N gene, integrated from 10 sources (see all 167):
    (articles sorted by number of sources associating them with UBE2N)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product. (PubMed id 8902611)1, 2, 3 Yamaguchi T....Kato S. (J. Biochem. 1996)
    2. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation. (PubMed id 16215985)1, 2, 9 Plans V.... Thomson T.M. (J. Cell. Biochem. 2006)
    3. ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity. (PubMed id 16112642)1, 2, 9 Takeuchi T. and Yokosawa H. (Biochem. Biophys. Res. Commun. 2005)
    4. The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains. (PubMed id 19340006)1, 2, 9 Marteijn J.A.... van der Reijden B.A. (Leukemia 2009)
    5. Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. (PubMed id 18615712)1, 2, 9 Scheper J.... Thomson T.M. (Proteins 2009)
    6. Covalent protein modification with ISG15 via a conserved cysteine in the hinge region. (PubMed id 22693631)1, 2 Bade V.N....Praefcke G.J. (PLoS ONE 2012)
    7. The mechanism of OTUB1-mediated inhibition of ubiquitination. (PubMed id 22367539)1, 2 Wiener R.... Wolberger C. (Nature 2012)
    8. Molecular insights into the function of RING Finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation. (PubMed id 22589545)1, 2 Campbell S.J.... Glover J.N. (J. Biol. Chem. 2012)
    9. TRIM5 is an innate immune sensor for the retrovirus capsid lattice. (PubMed id 21512573)1, 2 Pertel T.... Luban J. (Nature 2011)
    10. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. (PubMed id 20725033)1, 2 Nakada S.... Durocher D. (Nature 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 7334 HGNC: 12492 AceView: UBE2N Ensembl:ENSG00000177889 euGenes: HUgn7334
    ECgene: UBE2N Kegg: 7334 H-InvDB: UBE2N

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for UBE2N Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for UBE2N Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for UBE2N gene:
    Search GeneIP for patents involving UBE2N

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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