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Aliases for UBE2N Gene

Aliases for UBE2N Gene

  • Ubiquitin-Conjugating Enzyme E2N 2 3
  • Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13) 2 3
  • Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast) 2 3
  • Bendless-Like Ubiquitin-Conjugating Enzyme 3 4
  • Ubiquitin Carrier Protein N 3 4
  • Ubiquitin-Protein Ligase N 3 4
  • EC 6.3.2.19 4 63
  • UbcH13 3 4
  • UBC13 3 4
  • Bendless-Like Ubiquitin Conjugating Enzyme 3
  • Epididymis Secretory Protein Li 71 3
  • Ubiquitin-Conjugating Enzyme E2 N 3
  • Yeast UBC13 Homolog 3
  • UBCHBEN; UBC13 3
  • HEL-S-71 3
  • UbcH-Ben 3
  • BLU 4

External Ids for UBE2N Gene

Previous GeneCards Identifiers for UBE2N Gene

  • GC12M094324
  • GC12M093735
  • GC12M092305
  • GC12M092304
  • GC12M090868

Summaries for UBE2N Gene

Entrez Gene Summary for UBE2N Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2N Gene

UBE2N (Ubiquitin-Conjugating Enzyme E2N) is a Protein Coding gene. Among its related pathways are PI-3K cascade and Class I MHC mediated antigen processing and presentation. GO annotations related to this gene include ubiquitin-protein transferase activity and ubiquitin binding. An important paralog of this gene is UBE2T.

UniProtKB/Swiss-Prot for UBE2N Gene

  • The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the Lys-63-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the Lys-63-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate Lys-63-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).

Tocris Summary for UBE2N Gene

  • The�ubiquitin (Ub)/Ub-like (Ubl) conjugating enzymes�are a conserved family of ligases which mediate the conjugation of Ub and Ubl proteins to target substrates. There have been 35 E2 enzymes identified in the human genome to date.

Gene Wiki entry for UBE2N Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for UBE2N Gene

Genomics for UBE2N Gene

Regulatory Elements for UBE2N Gene

Genomic Location for UBE2N Gene

Start:
93,405,673 bp from pter
End:
93,442,262 bp from pter
Size:
36,590 bases
Orientation:
Minus strand

Genomic View for UBE2N Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for UBE2N Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2N Gene

Proteins for UBE2N Gene

  • Protein details for UBE2N Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61088-UBE2N_HUMAN
    Recommended name:
    Ubiquitin-conjugating enzyme E2 N
    Protein Accession:
    P61088
    Secondary Accessions:
    • Q16781
    • Q53Y81

    Protein attributes for UBE2N Gene

    Size:
    152 amino acids
    Molecular mass:
    17138 Da
    Quaternary structure:
    • Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific Lys-63-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the Lys-63-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Interacts with OTUB1; leading to inhibit E2-conjugating activity.

    Three dimensional structures from OCA and Proteopedia for UBE2N Gene

neXtProt entry for UBE2N Gene

Proteomics data for UBE2N Gene at MOPED

Selected DME Specific Peptides for UBE2N Gene

Post-translational modifications for UBE2N Gene

  • Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2
  • Ubiquitination at Lys10, Lys24, Lys68, Lys74, Lys82, Lys92, and Lys94
  • Modification sites at PhosphoSitePlus

Other Protein References for UBE2N Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for UBE2N Gene

Gene Families for UBE2N Gene

HGNC:
  • UBE2 :Ubiquitin-conjugating enzymes E2

Protein Domains for UBE2N Gene

Suggested Antigen Peptide Sequences for UBE2N Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61088

UniProtKB/Swiss-Prot:

UBE2N_HUMAN :
  • P61088
Family:
  • Belongs to the ubiquitin-conjugating enzyme family.
genes like me logo Genes that share domains with UBE2N: view

Function for UBE2N Gene

Molecular function for UBE2N Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
UniProtKB/Swiss-Prot EnzymeRegulation: Activity is inhibited by binding to OTUB1, which prevents Lys-63-linked polyubiquitination.
UniProtKB/Swiss-Prot Function: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the Lys-63-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the Lys-63-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate Lys-63-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).

Enzyme Numbers (IUBMB) for UBE2N Gene

Gene Ontology (GO) - Molecular Function for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IDA 20061386
GO:0005515 protein binding IPI 11473255
GO:0005524 ATP binding IEA --
GO:0016874 ligase activity IEA --
GO:0016881 acid-amino acid ligase activity --
genes like me logo Genes that share ontologies with UBE2N: view
genes like me logo Genes that share phenotypes with UBE2N: view

Animal Models for UBE2N Gene

MGI Knock Outs for UBE2N:

No data available for Transcription Factor Targeting and HOMER Transcription for UBE2N Gene

Localization for UBE2N Gene

Subcellular locations from UniProtKB/Swiss-Prot for UBE2N Gene

Nucleus. Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for UBE2N Gene COMPARTMENTS Subcellular localization image for UBE2N gene
Compartment Confidence
nucleus 5
cytosol 4
mitochondrion 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IDA 16307917
GO:0005634 nucleus IDA 16129784
GO:0005737 cytoplasm TAS 16129784
GO:0005829 cytosol TAS --
GO:0031372 UBC13-MMS2 complex IDA 16129784
genes like me logo Genes that share ontologies with UBE2N: view

Pathways for UBE2N Gene

genes like me logo Genes that share pathways with UBE2N: view

UniProtKB/Swiss-Prot P61088-UBE2N_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for UBE2N Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000724 double-strand break repair via homologous recombination IMP 17349954
GO:0000729 DNA double-strand break processing IMP 17349954
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS --
GO:0002224 toll-like receptor signaling pathway TAS --
GO:0002755 MyD88-dependent toll-like receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with UBE2N: view

Compounds for UBE2N Gene

(2) Tocris Compounds for UBE2N Gene

Compound Action Cas Number
Bay 11-7821 E2 ubiquitin (Ub) conjugating enzymes inhibitor [19542-67-7]
NSC 697923 Selective UBE2N inhibitor [343351-67-7]

(4) HMDB Compounds for UBE2N Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine monophosphate
  • 5'-AMP
61-19-8
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
Phosphoric acid
  • acide phosphorique (FRENCH)
7664-38-2
Pyrophosphate
  • (4-)Diphosphoric acid ion
14000-31-8
genes like me logo Genes that share compounds with UBE2N: view

Transcripts for UBE2N Gene

Unigene Clusters for UBE2N Gene

Ubiquitin-conjugating enzyme E2N:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2N Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c · 7d
SP1: -
SP2: - - -
SP3: - - - -
SP4: - -
SP5: - - -
SP6: - -

Relevant External Links for UBE2N Gene

GeneLoc Exon Structure for
UBE2N
ECgene alternative splicing isoforms for
UBE2N

Expression for UBE2N Gene

mRNA expression in normal human tissues for UBE2N Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for UBE2N Gene

SOURCE GeneReport for Unigene cluster for UBE2N Gene Hs.524630

genes like me logo Genes that share expressions with UBE2N: view

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for UBE2N Gene

Orthologs for UBE2N Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2N Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia UBE2N 35
  • 100 (n)
  • 100 (a)
UBE2N 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia UBE2N 35
  • 98.25 (n)
  • 100 (a)
UBE2N 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia UBE2N 35
  • 98.46 (n)
  • 100 (a)
UBE2N 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ube2n 35
  • 97.59 (n)
  • 100 (a)
Ube2n 16
Ube2n 36
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia UBE2N 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia UBE2N 36
  • 95 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ube2n 35
  • 97.15 (n)
  • 99.34 (a)
chicken
(Gallus gallus)
Aves UBE2N 35
  • 92.54 (n)
  • 99.34 (a)
UBE2N 36
  • 99 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2N 36
  • 100 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia ube2n-prov 35
tropical clawed frog
(Silurana tropicalis)
Amphibia MGC75672 35
ube2n 35
  • 89.04 (n)
  • 98.03 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8375 35
zebrafish
(Danio rerio)
Actinopterygii Dr.5512 35
ube2na 35
  • 85.53 (n)
  • 98.03 (a)
ube2na 36
  • 94 (a)
ManyToMany
ube2nb 36
  • 94 (a)
ManyToMany
fruit fly
(Drosophila melanogaster)
Insecta CG3473 35
  • 70.64 (n)
  • 78.81 (a)
BG:DS01486.1 37
  • 83 (a)
ben 37
  • 80 (a)
worm
(Caenorhabditis elegans)
Secernentea ubc-13 35
  • 70.95 (n)
  • 89.19 (a)
ubc-13 36
  • 85 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC13 36
  • 66 (a)
OneToMany
UBC13 38
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.508 35
thale cress
(Arabidopsis thaliana)
eudicotyledons UBC36 35
  • 66.44 (n)
  • 80.27 (a)
rice
(Oryza sativa)
Liliopsida Os.14354 35
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3622 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 71 (a)
OneToMany
Species with no ortholog for UBE2N:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for UBE2N Gene

ENSEMBL:
Gene Tree for UBE2N (if available)
TreeFam:
Gene Tree for UBE2N (if available)

Paralogs for UBE2N Gene

Paralogs for UBE2N Gene

Pseudogenes.org Pseudogenes for UBE2N Gene

genes like me logo Genes that share paralogs with UBE2N: view

Variants for UBE2N Gene

Sequence variations from dbSNP and Humsavar for UBE2N Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type MAF
rs471862 -- 93,425,978(-) TCCCA(A/C)AACCT intron-variant
rs486398 -- 93,423,173(+) TGAGC(A/G)CTGAA intron-variant
rs494999 -- 93,425,764(-) TTTGG(A/T)CAGAC intron-variant
rs506911 -- 93,413,665(-) ggcta(A/G)atgtg intron-variant
rs524497 -- 93,427,787(-) TTGAA(A/G)AAAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for UBE2N Gene

Variant ID Type Subtype PubMed ID
nsv7234 OTHER Inversion 18451855
esv272008 CNV Insertion 20981092
nsv823 CNV Loss 18451855
nsv528151 CNV Loss 19592680

Relevant External Links for UBE2N Gene

HapMap Linkage Disequilibrium report
UBE2N

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for UBE2N Gene

Disorders for UBE2N Gene

Relevant External Links for UBE2N

Genetic Association Database (GAD)
UBE2N
Human Genome Epidemiology (HuGE) Navigator
UBE2N
genes like me logo Genes that share disorders with UBE2N: view

No data available for UniProtKB/Swiss-Prot for UBE2N Gene

Publications for UBE2N Gene

  1. ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity. (PMID: 16112642) Takeuchi T. … Yokosawa H. (Biochem. Biophys. Res. Commun. 2005) 3 4 23
  2. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation. (PMID: 16215985) Plans V. … Thomson T.M. (J. Cell. Biochem. 2006) 3 4 23
  3. Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. (PMID: 18615712) Scheper J. … Thomson T.M. (Proteins 2009) 3 4 23
  4. The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains. (PMID: 19340006) Marteijn J.A. … van der Reijden B.A. (Leukemia 2009) 3 4 23
  5. Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product. (PMID: 8902611) Yamaguchi T. … Kato S. (J. Biochem. 1996) 2 3 4

Products for UBE2N Gene

  • Addgene plasmids for UBE2N

Sources for UBE2N Gene

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