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UBE2L6 Gene

protein-coding   GIFtS: 62

GC11M057075
ubiquitin-conjugating enzyme E2L 6
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.3.2.19 3
MGC40331 2
RIG-B 2, 3
UBCH8 1, 2, 3, 5
UbcH8 3
Descriptions
Retinoic acid-induced gene B protein 3
Ubiquitin carrier protein L6 3
Ubiquitin-protein ligase L6 3
retinoic acid induced gene B protein 2
ubiquitin carrier protein 2
ubiquitin-conjugating enzyme E2L 6 2
ubiquitin-protein ligase 2
External Ids
HGNC: 124901
Entrez Gene: 92462
UniProtKB: O149333
Ensembl: ENSG000001565877
Search outside databases for aliases for UBE2L6 gene

Previous GC identifers: GC11M059713 GC11M058832 GC11M057571 GC11M057094

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for UBE2L6:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting
abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes
of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and
ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme
family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.
Two alternatively spliced transcript variants encoding distinct isoforms have been found for this
gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933
Function: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in
the E6/E6-AP-induced ubiquitination of p53/TP53

Gene Wiki entry for UBE2L6

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2L6 gene  

Entrez Gene cytogenetic band: 11q12   Ensembl cytogenetic band:  11q12.1   HGNC cytogenetic band: 11q12.1

UBE2L6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M057075:     (about GC identifiers)

Start:
57,075,705 bp from pter
End:
57,103,217 bp from pter
Size:
27,513 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_033903.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933 (See protein sequence)
Recommended Name: Ubiquitin/ISG15-conjugating enzyme E2 L6  
Size: 153 amino acids; 17769 Da
Subunit: Interacts with RNF19A, RNF19B and RNF144B
PDB structure from and Proteopedia :
1WZV (3D)    1WZW (3D)    
Secondary accessions: A6NDM6 A8MY53 Q8N5D8 Q9UEZ0
Alternative splicing: 2 isoforms:  O14933-1   O14933-2   

REFSEQ proteins (2 alternative transcripts): 
NP_004214.1  NP_937826.1  


ENSEMBL proteins: 
ENSP00000341980 ENSP00000378545 ENSP00000287156 ENSP00000378547 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Enzo Life Sciences proteins for UBE2L6
Recombinant Proteins from Abcam (Ube2L6)
Human Recombinant Proteins from Abnova (UBE2L6)
               OriGene Purified Recombinant Human Proteins (see all 2): UBE2L6  

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IDA18029348
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Antibodies for UBE2L6: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for UBE2L6
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Antibodies from Abcam (Ube2L6), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2L6)
Novus Biologicals Antibodies for UBE2L6

Assays for UBE2L6: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Enzo Life Sciences assays for UBE2L6

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like


   GeneDecks  UBE2L6 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O14933

ProtoNet protein and cluster: O14933

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933
Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2L6)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2L6)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_198183

              Applied Biosystems Silencer® siRNAs for UBE2L6

              Sigma-Aldrich siRNA and siRNA Panels for UBE2L6  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2L6  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for UBE2L6
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004223
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004223
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_004223 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_004223

UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933
Function: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in
the E6/E6-AP-induced ubiquitination of p53/TP53
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
N-ubiquityllysine
Induction: By interferon beta
Enzyme Number (IUBMB): EC 6.3.2.19 

Genatlas biochemistry entry for UBE2L6:
ubiquitin conjugating enzyme H8,identified in a two hybrid screen through specific interaction with
E6AP (E3) ubiquitin protein ligase in the E6-dependent ubiquination of TP53

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004842 ubiquitin-protein ligase activity TAS9153201
GO:0005515 protein binding IPI10085091
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for UBE2L6 (Maps provided by GeneGo):
 Putative ubiquitin pathway

   GeneDecks  UBE2L6 for the pathways selected above  
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2 Millipore Pathways for UBE2L6
 Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway
 Proteolysis Putative ubiquitin pathway

   GeneDecks  UBE2L6 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2L6  (Your Favorite Gene powered by Ingenuity) 
 Hypoxia Signaling in the Cardiovascular System
 Protein Ubiquitination Pathway

   GeneDecks  UBE2L6 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for UBE2L6):
 hsa04120 Ubiquitin mediated proteolysis
 hsa05012 Parkinson's disease

   GeneDecks  UBE2L6 for the pathways selected above  
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UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933
Pathway: Protein modification; protein ubiquitination

 Gene Network CentralTM Interacting Genes and Proteins Network for  UBE2L6 


5/15 Interacting proteins for UBE2L6 (ENSP000002871563 O149332) via UniProtKB, MINT, and/or STRING (see all 15 )

InteractantInteraction Details
GeneCardExternal ID(s)
RNF144BQ7Z4192MINT-1771917 MINT-1771944
NAMPTP434902MINT-6538769
ISG15ENSP000003686993STRING (score=.952)
RNF19AENSP000003426673STRING (score=.894)
ARIH1ENSP000003692173STRING (score=.805)
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3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0019941 modification-dependent protein catabolic process IEA--
GO:0043687 post-translational protein modification IEA--
GO:0051246 regulation of protein metabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2L6

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2L6)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2L6)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_198183

              Sigma-Aldrich siRNA and siRNA Panels for UBE2L6  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2L6  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_004223  NM_198183  

REFSEQ mRNAs for UBE2L6 gene (2 alternative transcripts): 

NM_004223.3   NM_198183.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_004223  NM_198183  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004223
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004223
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_004223 

Additional cDNA sequence: 

AF031141.1 AF061736.1 AK093462.1 AK129621.1 AK226165.1 AK315755.1 BC032491.1 CR457126.1 
CR601863.1 CR609116.1 

11 DOTS entries:

DT.447411  DT.100006077  DT.92410683  DT.101981816  DT.91754122  DT.95301343  DT.95301290  DT.91754123 
DT.91754124  DT.91824239  DT.95301342 

24/394 AceView cDNA sequences (see all 394 ):

BQ069800 AI913308 BQ051009 CA307378 CD368787 BQ774439 BP338365 CA445308 
AW956195 BU733098 AI445428 BI914452 AL560616 AA779527 CB122118 T35558 
BC032491 BF796635 BU625117 AI880045 NM_004223 BE748060 BU181223 CA314466 

highest scoring ESTs for UBE2L6:

AW051910 AA883502 AI300033 AI300034 AI796357 AK093462 AL110315 AA012913 AA015915 AA015916 

Unigene Cluster for UBE2L6:

Ubiquitin-conjugating enzyme E2L 6
Hs.425777  [show with all ESTs]
Unigene Representative Sequence: AK093462


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2L6

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d · 6e
SP1:        -     -     -     -                                             
SP2:              -     -     -                                             
SP3:                    -     -                                             
SP4:                          -                                             
SP5:                                                                        

About this scheme

ECgene alternative splicing isoforms for UBE2L6

4 Ensembl transcripts including schematic representations:
ENST00000340573  ENST00000395113  ENST00000287156  ENST00000395115  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2L6 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2L6

1 / 2 / 3

4 probe-sets matching UBE2L6 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

75081_r_at2, 3 U95-D 1 1.00 1.00 0.05 0.84 AW051910 1.00 1.00 1.00 1
40505_at2, 3 U95-A 1 0.81 1.00 0.90 1.16 AA883502 0.80 1.00 0.91 1

201649_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004223 0.60 1.00 0.82 1

201649_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  UBE2L6 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTCTTGCTTA

SOURCE GeneReport for Unigene cluster: Hs.425777

Expression variation in blood from EXPOLDB for UBE2L6

UniProtKB/Swiss-Prot: UB2L6_HUMAN, O14933
Tissue specificity: Present in natural killer cells (at protein level)

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for UBE2L6 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
UBE2L61   -- ubiquitin-conjugating enzyme E2L 6 79.96(n)
70.59(a)
475967  XM_533174.2  XP_533174.1 
chimpanzee
(Pan troglodytes)
UBE2L61   -- ubiquitin-conjugating enzyme E2L 6 99.34(n)
100(a)
466588  XM_521987.2  XP_521987.2 
cow
(Bos taurus)
UBE2L61   -- ubiquitin-conjugating enzyme E2L 6 78.87(n)
69.93(a)
509471  XM_586436.3  XP_586436.3 
rat
(Rattus norvegicus)
Ube2l61   -- ubiquitin-conjugating enzyme E2L 6 78.87(n)
73.86(a)
295704  NM_001024755.1  NP_001019926.1 
mouse
(Mus musculus)
Ube2l61, 5 25
ubiquitin-conjugating enzyme E2L 61, 5 79.61(n)1
75.66(a)1
567911  NM_019949.11  NP_064333.11 
 AF1592305  AK0109425  (see all 17)
About this table        Species with no ortholog for UBE2L6

ENSEMBL Gene Tree for UBE2L6
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for UBE2L6 gene
UBE2S2  UBE2I2  UBE2L32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/126 NCBI SNPs in UBE2L6 are shown (see all 126 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 40)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs58471,2
A,C,F,H,O57075915(+) ATAAAC/TGGTTT 2 -- ut3114Minor allele frequency- A:0.01MN EA NA EU WA 2734
rs37410831,2
C,F,H57075963(-) CAACTG/AAGTCA 2 -- ut31 ese312Minor allele frequency- A:0.03EA NA EU WA 2428
--
rs27293701,2
A,C,H57075519(+) GGGACA/GTCACC 2 -- ng514Minor allele frequency- G:0.00EU EA WA 420
rs22344051,2
C57091968(-) ACGGCA/GCACCC 2 -- ng31 ut51 ese30--------
rs26496491,2
A,C,F,H,O57085289(-) TCTTCG/CGGAGT 2 -- int116Minor allele frequency- C:0.49EA NA EU WA 1474
rs49388711,2
A,C,F,H57085978(+) ACAAAC/Acagtg 2 -- int18Minor allele frequency- A:0.20EU EA WA 838
rs22344091,2
A,C,F,H57078387(-) AAGAGC/AGGAGA 2 -- int1 trp311Minor allele frequency- A:0.37EA NA EU WA 2320
--
rs49391511,2
A,C,F,H57091006(+) CCCCCC/GCCTCC 2 -- int1 trp34Minor allele frequency- G:0.17EU EA WA 412
rs11151721,2
A,C,F,H57086849(-) CAGCCA/GTGTCT 2 -- int19Minor allele frequency- C:0.06NS EU EA WA 572
rs26496511,2
A,C,F,H57083679(-) TAGGAT/CTTGGA 2 -- int12Minor allele frequency- C:0.44EU WA 200
About this table

HapMap Linkage Disequilibrium images for UBE2L6 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 603890

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/26 PubMed articles for UBE2L6 gene (see all 26 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9246 HGNC: 12490 AceView: UBE2L6 Ensembl:ENSG00000156587 euGenes: HUgn9246
ECgene: UBE2L6 H-InvDB: UBE2L6
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2L6:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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