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UBE2K Gene

protein-coding   GIFtS: 61
GCID: GC04P039700

Ubiquitin-Conjugating Enzyme E2K

(Previous names: huntingtin interacting protein 2, ubiquitin-conjugating...)
(Previous symbol: HIP2)
Microbiology & Infectious Diseases Congress
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ubiquitin-Conjugating Enzyme E2K1 2     Ubiquitin-Protein Ligase2 3
HIP21 2 3 5     HIP-22 3
Huntingtin Interacting Protein 21 2     LIG2 3
Ubiquitin-Conjugating Enzyme E2K (UBC1 Homolog, Yeast)1 2     EC 6.3.2.193 8
Huntingtin-Interacting Protein 22 3     E2(25K)2
Ubiquitin Carrier Protein2 3     E2-25K2
Ubiquitin-Conjugating Enzyme E2(25K)2 3     HYPG2
Ubiquitin-Conjugating Enzyme E2-25 KDa2 3     UBC12
Ubiquitin-Conjugating Enzyme E2-25K2 3     Ubiquitin-Conjugating Enzyme E2 K2

External Ids:    HGNC: 49141   Entrez Gene: 30932   Ensembl: ENSG000000781407   OMIM: 6028465   UniProtKB: P610863   

Export aliases for UBE2K gene to outside databases

Previous GC identifers: GC04P039377 GC04P039024


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for UBE2K Gene:
The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with
RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known
functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the
suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I
heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants
encoding different isoforms have been identified for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for UBE2K Gene:
UBE2K (ubiquitin-conjugating enzyme E2K) is a protein-coding gene. Diseases associated with UBE2K include pancoast tumor, and huntington's disease. GO annotations related to this gene include ubiquitin-protein ligase activity and ubiquitin-ubiquitin ligase activity.

UniProtKB/Swiss-Prot: UBE2K_HUMAN, P61086
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro,
in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of
'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated
substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the
endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May
mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination
and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of
NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved
in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol.
In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1

Gene Wiki entry for UBE2K (HIP2) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000004.12  NC_018915.2  NT_006238.12  
Regulatory elements:
   Regulatory transcription factor binding sites in the UBE2K gene promoter:
         Nkx3-1   ER-alpha   Nkx3-1 v4   AP-1   ATF-2   Nkx3-1 v1   Nkx3-1 v2   Nkx3-1 v3   IRF-7A   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidUBE2K promoter sequence
   Search Chromatin IP Primers for UBE2K

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat UBE2K


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4p14   Ensembl cytogenetic band:  4p14   HGNC cytogenetic band: 4p14

UBE2K Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
UBE2K gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P039700:  view genomic region     (about GC identifiers)

Start:
39,699,664 bp from pter      End:
39,784,412 bp from pter
Size:
84,749 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: UBE2K_HUMAN, P61086 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 K  
Size: 200 amino acids; 22407 Da
Subunit: Interacts with RNF138/NARF. Interacts with BRCA1
6 PDB 3D structures from and Proteopedia for UBE2K:
1YLA (3D)        2O25 (3D)        3E46 (3D)        3F92 (3D)        3K9O (3D)        3K9P (3D)    
Secondary accessions: A6NJC1 A8K5Y9 B2RDF8 C9JGP1 O54806 P27924 Q16721 Q9CVV9 Q9Y2D3
Alternative splicing: 3 isoforms:  P61086-1   P61086-2   P61086-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for UBE2K: NX_P61086

Explore proteomics data for UBE2K at MOPED

Post-translational modifications: 

  • Sumoylation at Lys-14 impairs catalytic activity (By similarity)1
  • Ubiquitination2 at Lys72, Lys165
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for UBE2K (P61086) (see all 5)
     ICLDILK  GPPDTPY  RIKREFKEV  TLRTVLLSLQA 


    See UBE2K Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001104582.1  NP_001104583.1  NP_005330.1  

    ENSEMBL proteins: 
     ENSP00000261427   ENSP00000425301   ENSP00000421203   ENSP00000390483   ENSP00000421422  
     ENSP00000295963  
    Reactome Protein details: P61086

    UBE2K Human Recombinant Protein Products:

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    Enzo Life Sciences proteins for UBE2K
    OriGene Purified Proteins for UBE2K
    OriGene Protein Over-expression Lysate for UBE2K
    OriGene MassSpec for UBE2K
    OriGene Custom Protein Services for UBE2K
    GenScript Purified and Recombinant Proteins for UBE2K
    Novus Biologicals UBE2K Proteins
    Novus Biologicals UBE2K Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for UBE2K
    Browse Proteins at Cloud-Clone Corp.

    UBE2K Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for UBE2K (UBE2K/E2-25K)
    Cell Signaling Technology (CST) Antibodies for UBE2K 
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    Novus Biologicals UBE2K Antibodies
    Abcam antibodies for UBE2K
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    ThermoFisher Antibody for UBE2K
    LSBio Antibodies in human, mouse, rat for UBE2K

    UBE2K Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for UBE2K
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    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    UBE2: Ubiquitin-conjugating enzymes E2

    Selected InterPro protein domains (see all 6):
     IPR023313 UBQ-conjugating_AS
     IPR000608 UBQ-conjugat_E2
     IPR009060 UBA-like
     IPR016135 UBQ-conjugating_enzyme/RWD
     IPR000449 UBA/Ts_N

    Graphical View of Domain Structure for InterPro Entry P61086

    ProtoNet protein and cluster: P61086

    UniProtKB/Swiss-Prot: UBE2K_HUMAN, P61086
    Similarity: Belongs to the ubiquitin-conjugating enzyme family
    Similarity: Contains 1 UBA domain


    UBE2K for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: UBE2K_HUMAN, P61086
    Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro,
    in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of
    'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated
    substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the
    endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May
    mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination
    and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of
    NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved
    in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol.
    In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
    Induction: By aggregated low-density lipoprotein

         Enzyme Number (IUBMB): EC 6.3.2.191 2

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity IDA--
    GO:0005515protein binding IPI17500595
    GO:0005524ATP binding IEA--
    GO:0016881acid-amino acid ligase activity ----
    GO:0031625ubiquitin protein ligase binding IPI16714285
         
    UBE2K for ontologies           About GeneDecksing


    Phenotypes:
         4 GenomeRNAi human phenotypes for UBE2K:
     Cell division defect  Increased 8N DNA content  Increased cell death HMECs cel  Synthetic lethal with Ras 

         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Ube2k):
     cellular  nervous system 

    UBE2K for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for UBE2K
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for UBE2K

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for UBE2K
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for UBE2K

    miRNA
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    miRTarBase miRNAs that target UBE2K:
    hsa-mir-122-5p (MIRT023304), hsa-mir-186-5p (MIRT044916), hsa-mir-26b-5p (MIRT029298)

    Block miRNA regulation of human, mouse, rat UBE2K using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate UBE2K (see all 105):
    hsa-miR-548j hsa-miR-361-5p hsa-miR-4272 hsa-miR-128 hsa-miR-30d hsa-miR-30a hsa-miR-298 hsa-miR-548a-5p
    SwitchGear 3'UTR luciferase reporter plasmidUBE2K 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for UBE2K
    Predesigned siRNA for gene silencing in human, mouse, rat UBE2K

    Gene Editing
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    Clone
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    OriGene clones in human, mouse for UBE2K (see all 16)
    OriGene ORF clones in mouse, rat for UBE2K
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    GenScript: all cDNA clones in your preferred vector (see all 3): UBE2K (NM_005339)
    Sino Biological Human cDNA Clone for UBE2K
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for UBE2K
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat UBE2K

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for UBE2K 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for UBE2K


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    UBE2K_HUMAN, P61086: Cytoplasm (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol3
    nucleus2
    cytoskeleton1
    endoplasmic reticulum1
    extracellular1
    mitochondrion1
    plasma membrane1

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--

    UBE2K for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for UBE2K About    
    See pathways by source

    SuperPathContained pathways About
    1Class I MHC mediated antigen processing and presentation
    Class I MHC mediated antigen processing and presentation0.84
    Adaptive Immune System0.41
    Antigen processing- Ubiquitination and Proteasome degradation0.84
    Ubiquitin mediated proteolysis0.35
    2RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
    RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways0.47
    Negative regulators of RIG-I/MDA5 signaling0.42
    3Protein Stability
    Protein Stability
    4Neuroscience
    Neuroscience

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 Cell Signaling Technology (CST) Pathways for UBE2K
        Neuroscience
    Protein Stability


    2 Reactome Pathways for UBE2K
        Antigen processing: Ubiquitination & Proteasome degradation
    Negative regulators of RIG-I/MDA5 signaling


    1 Kegg Pathway  (Kegg details for UBE2K):
        Ubiquitin mediated proteolysis

    UniProtKB/Swiss-Prot: UBE2K_HUMAN, P61086
    Pathway: Protein modification; protein ubiquitination


    UBE2K for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including UBE2K: 

              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Parkinson's Disease in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for UBE2K

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for UBE2K (P610861, 2, 3 ENSP000002614274) via UniProtKB, MINT, STRING, and/or I2D (see all 194)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PIAS4Q8N2W92, 3, ENSP000002629714MINT-3980642 I2D: score=4 STRING: ENSP00000262971
    ITSN1Q158112, 3, ENSP000003707194MINT-8276207 I2D: score=2 STRING: ENSP00000370719
    PRPF40AO754002, 3MINT-8276218 I2D: score=2 
    ENSG00000224994Q9HCM93, ENSP000004119494I2D: score=1 STRING: ENSP00000411949
    ENSG00000232839Q9HCM93, ENSP000003983554I2D: score=1 STRING: ENSP00000398355
    About this table

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006511ubiquitin-dependent protein catabolic process TAS8702625
    GO:0032434regulation of proteasomal ubiquitin-dependent protein catabolic process IEA--
    GO:0070059intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress IEA--
    GO:0070936protein K48-linked ubiquitination IDA--

    UBE2K for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for UBE2K

    6 Novoseek inferred chemical compound relationships for UBE2K gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    lysine 34.4 1 14517261 (1)
    cysteine 23.6 5 16342931 (3), 11504715 (1), 14517261 (1)
    actinomycin d 16.3 1 9685415 (1)
    atp 7 2 8034027 (1), 16275321 (1)
    tyrosine 0 1 10966114 (1)
    h2o2 0 3 12773310 (3)



    UBE2K for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for UBE2K gene (3 alternative transcripts): 
    NM_001111112.1  NM_001111113.1  NM_005339.4  

    Unigene Cluster for UBE2K:

    Ubiquitin-conjugating enzyme E2K
    Hs.50308  [show with all ESTs]
    Unigene Representative Sequence: NM_005339
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000261427(uc003guu.4 uc003gus.4 uc003gut.4 uc010ifn.3 uc011byq.2 uc003guq.4)
    ENST00000510934 ENST00000513231 ENST00000438068 ENST00000503368 ENST00000445950
    ENST00000510719 ENST00000295963
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Block miRNA regulation of human, mouse, rat UBE2K using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate UBE2K (see all 105):
    hsa-miR-548j hsa-miR-361-5p hsa-miR-4272 hsa-miR-128 hsa-miR-30d hsa-miR-30a hsa-miR-298 hsa-miR-548a-5p
    SwitchGear 3'UTR luciferase reporter plasmidUBE2K 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat UBE2K
    Clone
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    GenScript: all cDNA clones in your preferred vector (see all 3): UBE2K (NM_005339)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat UBE2K
    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat UBE2K
      QuantiTect SYBR Green Assays in human, mouse, rat UBE2K
      QuantiFast Probe-based Assays in human, mouse, rat UBE2K

    Additional mRNA sequence: 

    AB022435.1 AB022436.1 AK093533.1 AK291454.1 AK295846.1 AK307130.1 AK315524.1 AL117568.1 
    BC022804.1 BC050600.1 BX641089.1 U58522.1 

    21 DOTS entries:

    DT.92442814  DT.453166  DT.97780192  DT.95364285  DT.100718402  DT.75154564  DT.95364286  DT.100777751 
    DT.100813999  DT.95364298  DT.100763089  DT.91812239  DT.91828453  DT.95364296  DT.102834685  DT.40111771 
    DT.75187719  DT.91742775  DT.92442816  DT.92442819  DT.92442822 

    Selected AceView cDNA sequences (see all 407):

    BM791514 CA453659 AV753548 BM992652 AI074875 BI438859 AA747449 CR605719 
    BQ416785 BU689861 BU170325 BM479441 BQ016199 AW139164 AU134343 AA334137 
    BM561379 AV752976 BX427147 Z45197 AA970776 AA838816 BM839856 H23674 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for UBE2K (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7
    SP1:                          -                                                         
    SP2:                    -     -                                                         
    SP3:                          -                                   -     -     -         
    SP4:                          -     -                                                   
    SP5:                          -     -     -                                             


    ECgene alternative splicing isoforms for UBE2K

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    UBE2K expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGATGTAGAG
    UBE2K Expression
    About this image


    UBE2K expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 12) fully expand
     
     Brain (Nervous System)    fully expand to see all 7 entries
             Cerebral Cortex
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Metencephalon
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Lung (Respiratory System)    fully expand to see all 2 entries
             Stem Bronchi
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
    UBE2K Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    UBE2K Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.50308

    UniProtKB/Swiss-Prot: UBE2K_HUMAN, P61086
    Tissue specificity: Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small
    intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear
    cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in
    cerebellum and brainstem

        Pathway & Disease-focused RT2 Profiler PCR Arrays including UBE2K: 
              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Parkinson's Disease in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for UBE2K gene from Selected species (see all 27)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ube2k1 , 5 ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)5
    ubiquitin-conjugating enzyme E2K1
    95.67(n)1
    100(a)1
      5 (33.72 cM)5
    533231  NM_016786.31  NP_058066.21 
     655372335 
    chicken
    (Gallus gallus)
    Aves UBE2K1 ubiquitin-conjugating enzyme E2K 92.33(n)
    99.5(a)
      428787  NM_001031540.1  NP_001026711.1 
    lizard
    (Anolis carolinensis)
    Reptilia UBE2K6
    ubiquitin-conjugating enzyme E2K
    90(a)
    1 ↔ 1
    5(99793960-99812196)
    African clawed frog
    (Xenopus laevis)
    Amphibia hip2-prov2 huntingtin interacting protein 2 84.14(n)    BC041728.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ube2kb1 ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) 81.17(n)
    97(a)
      30100  NM_001008611.2  NP_001008611.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta UbcD41 Ubiquitin conjugating enzyme 4 62.98(n)
    68.34(a)
      39133  NM_079286.3  NP_524010.2 
    worm
    (Caenorhabditis elegans)
    Secernentea ubc-201 ubc-20 56.28(n)
    52.26(a)
      175185  NM_064773.4  NP_497174.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes UBC11 UBC1 52.2(n)
    43.92(a)
      851757   NP_010462.3 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons UBC271 UBC27 54.04(n)
    51.05(a)
      835159  NM_001203579.1  NP_001190508.1 
    rice
    (Oryza sativa)
    Liliopsida Os.186732 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 80.85(n)    AK073357.1 


    ENSEMBL Gene Tree for UBE2K (if available)
    TreeFam Gene Tree for UBE2K (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for UBE2K gene
    Selected SIMAP similar genes for UBE2K using alignment to 5 protein entries:     UBE2K_HUMAN (see all proteins) (see all similar genes):
    HIP2    UBE2D3    UBE2D2    UBE2D1    UBE2D4    UBE2M
    UBE2H    UBE2A    UBE2B    UBE2NL    UBE2C    UBE2E3
    UBE2G1    UBE2S    UBE2E1    UBE2E2    UBE2L3    UBE2T

    UBE2K for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for UBE2K
    PGOHUM00000245096


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for UBE2K (see all 1999)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1126202981,2
    C--39023979(+) TGTGT-/GAG   
       AGAGA
    GAGAG
    3 -- us2k11Minor allele frequency- GAGAGAGA:0.00CSA 2
    rs572892681,2
    C--39079030(+) TTTTT-/T/TT  
            
    GAGAC
    3 -- int11NA 2
    rs1905462041,2
    --39697778(+) TGCCAC/TCACAA 3 -- us2k10--------
    rs1429450451,2
    --39697793(+) AATTAC/TTTTTG 3 -- us2k10--------
    rs76818671,2
    C,F,H--39697803(+) GGTAAG/AACCAT 3 -- us2k1 tfbs311Minor allele frequency- A:0.36NA WA CSA EA 376
    rs1510891021,2
    --39697995(+) ATTATC/GTTTAG 3 -- us2k10--------
    rs76571261,2
    C,F--39698107(+) ccaccG/Acgccc 3 -- us2k11Minor allele frequency- A:0.07NA 120
    rs762092651,2
    F--39698150(+) TTTTGG/TTTTTT 3 -- us2k11Minor allele frequency- T:0.50NA 4
    rs76586791,2
    C,F,A--39698171(+) agtctT/Cgctcc 3 -- us2k15Minor allele frequency- C:0.40NA CSA 10
    rs76575021,2
    C,F--39698302(+) gcccaG/Cctatt 3 -- us2k13Minor allele frequency- C:0.33NA WA 6

    HapMap Linkage Disequilibrium report for UBE2K (39699664 - 39784412 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for UBE2K (see all 14):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2666876CNV Deletion23128226
    esv2669309CNV Deletion23128226
    esv2660659CNV Deletion23128226
    esv2727459CNV Deletion23290073
    nsv878948CNV Loss21882294
    nsv878954CNV Loss21882294
    dgv5528n71CNV Loss21882294
    nsv516320CNV Loss19592680
    nsv518644CNV Loss19592680
    nsv878949CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing UBE2K
    DNA2.0 Custom Variant and Variant Library Synthesis for UBE2K

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 602846    OMIM disorders: --

    Selected diseases for UBE2K (see all 21):    
    About MalaCards
    pancoast tumor    huntington's disease    liddle syndrome    cblc
    autistic disorder    leptospirosis    angelman syndrome    fanconi's anemia
    hepatitis c virus    hepatitis c    parkinson's disease    cerebritis
    atherosclerosis    breast cancer    tonsillitis    alzheimer's disease
    hepatitis    thyroiditis    prostatitis    malaria

    2 diseases from the University of Copenhagen DISEASES database for UBE2K:
    Angelman syndrome     Parkinson's disease

    UBE2K for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    1 Novoseek inferred disease relationship for UBE2K gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    huntington disease 28.5 2 10585161 (1)


    Export disorders for UBE2K gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for UBE2K gene, integrated from 10 sources (see all 150):
    (articles sorted by number of sources associating them with UBE2K)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. (PubMed id 17873885)1, 2, 3, 9 Christensen D.E.... Klevit R.E. (Nat. Struct. Mol. Biol. 2007)
    2. Huntingtin is ubiquitinated and interacts with a specific ubiquitin- conjugating enzyme. (PubMed id 8702625)1, 2, 3 Kalchman M.A.... Hayden M.R. (J. Biol. Chem. 1996)
    3. Induction of ubiquitin-conjugating enzyme by aggregated low density lipoprotein in human macrophages and its implications for atherosclerosis. (PubMed id 10634809)1, 2, 9 Kikuchi J.... Sakurabayashi I. (Arterioscler. Thromb. Vasc. Biol. 2000)
    4. E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system. (PubMed id 16868077)1, 2, 9 Flierman D.... Chau V. (Proc. Natl. Acad. Sci. U.S.A. 2006)
    5. NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF). (PubMed id 16714285)1, 2 Yamada M.... Shibuya H. (J. Biol. Chem. 2006)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. (PubMed id 12665801)1, 2 Gevaert K.... Vandekerckhove J. (Nat. Biotechnol. 2003)
    9. Regulation of macrophage-specific gene expression by degenerated lipoproteins. (PubMed id 10675012)1, 2 Furukawa Y....Sakurabayashi I. (Electrophoresis 2000)
    10. E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme. (PubMed id 11513855)1, 9 Lee S.-J.... Kang S. (FEBS Lett. 2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 3093 HGNC: 4914 AceView: HIP2 Ensembl:ENSG00000078140 euGenes: HUgn3093
    ECgene: UBE2K Kegg: 3093 H-InvDB: UBE2K

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for UBE2K Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for UBE2K gene:
    Search GeneIP for patents involving UBE2K

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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