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UBE2J2 Gene

protein-coding   GIFtS: 54

GC01M001182
ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.3.2.19 3
NCUBE2 1, 2, 3
OTTHUMP00000001907 2
OTTHUMP00000002078 2
PRO2121 2
Ubc6p 1
Descriptions
Non-canonical ubiquitin-conjugating enzyme 2 3
ubiquitin conjugating enzyme 6 2
ubiquitin conjugating enzyme E2, J2 2
ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) 2
yeast UBC6 homolog 2
External Ids
HGNC: 192681
Entrez Gene: 1184242
UniProtKB: Q8N2K13
Ensembl: ENSG000001600877
Search outside databases for aliases for UBE2J2 gene

Previous GC identifers: GC01M000941 GC01M001066 GC01M001095 GC01M001229

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for UBE2J2:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting
abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes
of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and
ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating
enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple
alternatively spliced transcript variants have been found for this gene, but the full-length
nature of some variants has not been defined. [provided by RefSeq]

UniProtKB/Swiss-Prot: UB2J2_HUMAN, Q8N2K1
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in
the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2J2 gene  

Entrez Gene cytogenetic band: 1p36.33   Ensembl cytogenetic band:  1p36.33   HGNC cytogenetic band: 1p36.33

UBE2J2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M001182:     (about GC identifiers)

Start:
1,179,155 bp from pter
End:
1,199,097 bp from pter
Size:
19,943 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_004350.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UB2J2_HUMAN, Q8N2K1 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 J2  
Size: 259 amino acids; 28925 Da
Subcellular location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein (By
similarity)
PDB structures from and Proteopedia :
2F4W (3D)    
Secondary accessions: Q96N26 Q96T84
Alternative splicing: 2 isoforms:  Q8N2K1-1   Q8N2K1-2   

REFSEQ proteins (4 alternative transcripts): 
NP_477515.2  NP_919296.1  NP_919439.1  NP_919440.1  


ENSEMBL proteins: 
ENSP00000342541 ENSP00000305826 ENSP00000353653 ENSP00000340197 ENSP00000383719 ENSP00000383718 
ENSP00000344857 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (UBC6)
Human Recombinant Proteins from Abnova (UBE2J2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005783 endoplasmic reticulum IEA--
GO:0005789 endoplasmic reticulum membrane IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

Antibodies for UBE2J2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for UBE2J2
Browse R&D Systems for Antibodies
Antibodies from Abcam (UBC6), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2J2)
Novus Biologicals Antibodies for UBE2J2

Assays for UBE2J2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like


   GeneDecks  UBE2J2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q8N2K1

ProtoNet protein and cluster: Q8N2K1

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UB2J2_HUMAN, Q8N2K1
Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2J2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2J2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_058167

              Applied Biosystems Silencer® siRNAs for UBE2J2

              Sigma-Aldrich siRNA and siRNA Panels for UBE2J2  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2J2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for UBE2J2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_058167
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_058167
                                 untagged cDNA clones in CMV expression vector (see all 6): AF296658 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_194315

UniProtKB/Swiss-Prot: UB2J2_HUMAN, Q8N2K1
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in
the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (By
similarity)
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
N-ubiquityllysine
Enzyme Number (IUBMB): EC 6.3.2.19 

1 MGI phenotypic allele for Ube2j2 (no phenotypes)


2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004842 ubiquitin-protein ligase activity IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Millipore Pathway for UBE2J2
 Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway

   GeneDecks  UBE2J2 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2J2  (Your Favorite Gene powered by Ingenuity) 
 Hypoxia Signaling in the Cardiovascular System
 Protein Ubiquitination Pathway

   GeneDecks  UBE2J2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for UBE2J2):
 hsa04120 Ubiquitin mediated proteolysis
 hsa05012 Parkinson's disease

   GeneDecks  UBE2J2 for the pathways selected above  
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UniProtKB/Swiss-Prot: UB2J2_HUMAN, Q8N2K1
Pathway: Protein modification; protein ubiquitination


1 Interacting protein for UBE2J2 (ENSP000003058263) via UniProtKB, MINT, and/or STRING

InteractantInteraction Details
GeneCardExternal ID(s)
UBA1ENSP000003384133STRING (score=.801)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0019941 modification-dependent protein catabolic process IEA--
GO:0043687 post-translational protein modification IEA--
GO:0051246 regulation of protein metabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2J2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2J2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2J2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_058167

              Sigma-Aldrich siRNA and siRNA Panels for UBE2J2  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2J2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_058167  NM_194315  NM_194457  NM_194458  

REFSEQ mRNAs for UBE2J2 gene (4 alternative transcripts): 

NM_058167.2   NM_194315.1   NM_194457.1   NM_194458.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_058167  NM_194315  NM_194457  NM_194458  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_058167
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_058167
                                 untagged cDNA clones in CMV expression vector (see all 6): AF296658 

Additional cDNA sequence: 

AF296658.1 AK056065.1 AK075017.1 AK098803.1 AK291935.1 BC027728.2 BC052579.1 BC094777.1 
BC104890.1 BC113430.1 BC143657.1 CR590185.1 CR592102.1 CR598255.1 CR601173.1 CR605285.1 
CR607169.1 CR610022.1 CR612438.1 

19 DOTS entries:

DT.100881363  DT.40283075  DT.100042059  DT.100881365  DT.419470  DT.425940  DT.95076950  DT.95193420 
DT.121442457  DT.95076949  DT.97836722  DT.121442506  DT.100809540  DT.100881349  DT.121442490  DT.91915343 
DT.95076884  DT.100881357  DT.95279782 

24/358 AceView cDNA sequences (see all 358 ):

BG391351 CD365765 BG421662 CR612438 BM766032 BG397868 AI140287 BG110522 
AW167553 BM765278 CB160932 AK098803 BM920381 AW404406 CK431124 NM_194457 
CR598255 BF037192 BE262422 BM473968 AI784467 AF116687 BE775090 BM794599 

highest scoring ESTs for UBE2J2:

AA314553 AA411279 AA442034 AI681334 AK075017 AL555293 BE382927 BE905217 BF035520 BF037192 

Unigene Cluster for UBE2J2:

Ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Hs.191987  [show with all ESTs]
Unigene Representative Sequence: NM_194458


GeneLoc Exon Structure

5/27 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2J2 (see all 27 )

ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b · 9c ^ 10a · 10b ^ 11a · 11b ^ 12a · 12b ·
SP1:              -     -     -     -     -                 -     -     -     -           -     -     -     -                       -     -                     
SP2:              -     -     -     -     -                 -     -     -                 -     -     -     -                       -     -                     
SP3:                                -     -                 -     -     -     -           -     -     -     -                       -     -                     
SP4:                                                        -     -     -                 -     -     -     -                       -     -                     
SP5:                                                        -     -     -     -           -           -     -                       -     -                     

ExUns: 12c · 12d · 12e · 12f · 12g · 12h · 12i · 12j · 12k · 12l
SP1:                                                            
SP2:                                                            
SP3:                                                            
SP4:                                                            
SP5:                                                            

About this scheme

ECgene alternative splicing isoforms for UBE2J2

7 Ensembl transcripts including schematic representations:
ENST00000348298  ENST00000349431  ENST00000360466  ENST00000339385  ENST00000400930  ENST00000400929  
ENST00000347370  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2J2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2J2

1 / 2 / 3

7 probe-sets matching UBE2J2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
44735_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI681334 0.80 1.00 0.91 1

225209_s_at2, 3 U133-B 1 1.00 1.00 -- -- BE962920 0.60 1.00 0.82 1

224347_x_at2, 3 U133-B 1 1.00 1.00 -- -- AF116687 0.40 1.00 0.76 1

226704_at2, 3 U133-B 1 1.00 1.00 -- -- R76659 0.60 1.00 0.82 1

225209_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

224347_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

226704_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  UBE2J2 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CGCCGGGGGC

SOURCE GeneReport for Unigene cluster: Hs.191987
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for UBE2J2 gene from 5/16 species (see all 16 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
UBE2J21   -- ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, more 89.58(n)
95.75(a)
606935  XM_843241.1  XP_848334.1 
cow
(Bos taurus)
UBE2J21   -- ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, more 89.7(n)
95.37(a)
506103  NM_001038043.1  NP_001033132.1 
rat
(Rattus norvegicus)
Ube2j21   -- ubiquitin-conjugating enzyme E2, J2 86.49(n)
94.98(a)
298689  NM_001007655.1  NP_001007656.1 
mouse
(Mus musculus)
Ube2j21, 5 4 (83.00 cM)5
ubiquitin-conjugating enzyme E2, J2 homolog (yeast)1, 5 86.87(n)1
94.21(a)1
1404991  NM_001039157.11  NP_001034246.11 
 AA5452215  AF2966565  (see all 21)
chicken
(Gallus gallus)
UBE2J21   -- ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, more 79.92(n)
91.51(a)
419421  XM_001233676.1  XP_001233677.1 
About this table        Species with no ortholog for UBE2J2

ENSEMBL Gene Tree for UBE2J2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for UBE2J2 gene
UBE2J12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/92 NCBI SNPs in UBE2J2 are shown (see all 92 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 43)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs10581611,2
C,H1180386(-) ACCTCG/AGCAGG 4 -- ut31 ese35Minor allele frequency- A:0.00MN EU EA WA 570
--
rs112605771,2
A,C1200334(+) CCCGCC/TCTGCC 4 -- ng310--------
rs37377221,2
A,C1199499(-) TCCACC/TGAGCC 4 -- ng310--------
rs37377231,2
F1199402(-) GAGCCT/GGGAGG 4 -- ng311Minor allele frequency- G:0.34EA 1286
rs344969351,2
F1193134(-) TGTGCC/TGAGCC 4 A/A syn1 int1 ut511Minor allele frequency- T:0.03NA 78
--
rs112605751,2
A1200215(+) GCCAAA/GCTGTT 4 -- ng310--------
--
rs112605761,2
A1200223(+) GTTTTC/TACAGC 4 -- ng310--------
rs121205411,2
H1178858(+) CTATTC/AGTTAG 4 -- ng514Minor allele frequency- A:0.00EU EA WA 420
rs40186081,2
A,C,F,H1193801(-) TGCCCA/GGTGAG 4 -- int112Minor allele frequency- G:0.38EU EA WA NA 1036
rs112605701,2
A,C,F,H1191503(+) ACACAT/CTTCCT 4 -- int14Minor allele frequency- C:0.06EU EA WA 414
About this table

HapMap Linkage Disequilibrium images for UBE2J2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for UBE2J2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 118424 HGNC: 19268 AceView: UBE2J2 Ensembl:ENSG00000160087 euGenes: HUgn118424
ECgene: UBE2J2 H-InvDB: UBE2J2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2J2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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UBE2J2 Gene at Home site.
Version 2.41.1
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