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UBE2H Gene

protein-coding   GIFtS: 63

GC07M129257
ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
(Previous name: ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases &
Descriptions
for UBE2H

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
E2-20K 2, 3
EC 6.3.2.19 3
UBC8 1, 2, 5
UBCH 1, 2
UBCH2 2, 5
UbcH2 3
Descriptions
Ubiquitin carrier protein H 2, 3
Ubiquitin-protein ligase H 2, 3
ubiquitin-conjugating enzyme E2H 2
ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 2
ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) 1, 2
External Ids
HGNC: 124841
Entrez Gene: 73282
UniProtKB: P622563
Ensembl: ENSG000001865917
Search outside databases for aliases for UBE2H gene

Previous GC identifers: GC07M127950 GC07M129009 GC07M129026 GC07M129064

Summaries
for UBE2H

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for UBE2H:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting
abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes
of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and
ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating
enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98%
identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have
been found for this gene and they encode distinct isoforms. [provided by RefSeq]

UniProtKB/Swiss-Prot: UBE2H_HUMAN, P62256
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to
ubiquitinate histone H2A

Gene Wiki entry for UBE2H

Genomic Location
for UBE2H

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2H gene  

Entrez Gene cytogenetic band: 7q32   Ensembl cytogenetic band:  7q32.2   HGNC cytogenetic band: 7q32

UBE2H Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M129257:     (about GC identifiers)

Start:
 129,260,231 bp from pter
End:
 129,380,025 bp from pter
Size:
 119,795 bases
Orientation:
 minus strand

1 alternative location:
Chr7-,CRA_TCAG 128,857,649-128,977,424     
RefSeq DNA sequence:
NC_000007.12  NT_007933.14  NT_079596.2  

Proteins
for UBE2H

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: UBE2H_HUMAN, P62256 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 H  
Size: 183 amino acids; 20655 Da
PDB structures from and Proteopedia :
2Z5D (3D)    
Secondary accessions: P37286

REFSEQ proteins (2 alternative transcripts): 
NP_003335.1  NP_874356.1  


ENSEMBL proteins: 
ENSP00000347836 ENSP00000346395 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Millipore Purified and/or Recombinant UBE2H Protein
Sigma-Aldrich Proteins for UBE2H  
Browse R&D Systems for human recombinant proteins
Enzo Life Sciences proteins for UBE2H
Recombinant Proteins from Abcam (Ube2H)
Human Recombinant Proteins from Abnova (UBE2H)
               OriGene Purified Recombinant Human Proteins (see all 2): UBE2H  

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005739 mitochondrion IDA18029348
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Antibodies for UBE2H: 
Browse Antibodies Central at Invitrogen
Millipore Mono- and Polyclonal Antibodies for the study of UBE2H
Sigma-Aldrich Antibodies for UBE2H
Browse R&D Systems for Antibodies
Antibodies from Abcam (Ube2H), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2H)
Novus Biologicals Antibody for UBE2H

Assays for UBE2H: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Enzo Life Sciences assays for UBE2H

Protein Domains/
Families
for UBE2H

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
3 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like
 IPR015581 Ubq_conj_E2


   GeneDecks  UBE2H for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P62256

ProtoNet protein and cluster: P62256

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UBE2H_HUMAN, P62256
Similarity: Belongs to the ubiquitin-conjugating enzyme family

Gene Function
for UBE2H

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (UBE2H)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2H)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003344

              Applied Biosystems Silencer® siRNAs for UBE2H

              Sigma-Aldrich siRNA and siRNA Panels for UBE2H  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2H  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003344
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003344
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003344 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_003344

UniProtKB/Swiss-Prot: UBE2H_HUMAN, P62256
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to
ubiquitinate histone H2A
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
N-ubiquityllysine
Enzyme Number (IUBMB): EC 6.3.2.19 

Genatlas biochemistry entry for UBE2H:
ubiquitin conjugating enzyme H2A,homolog to yeast UBC8

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004842 ubiquitin-protein ligase activity TAS8132613
GO:0016874 ligase activity IEA--
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Pathways & Interactions
for UBE2H

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Millipore Pathway for UBE2H
 Common schema of ubiquitination

   GeneDecks  UBE2H for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2H  (Your Favorite Gene powered by Ingenuity) 
 Protein Ubiquitination Pathway
 Hypoxia Signaling in the Cardiovascular System

   GeneDecks  UBE2H for the pathways selected above  
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1 Kegg Pathway  (Kegg details for UBE2H):
 hsa04120 Ubiquitin mediated proteolysis

   GeneDecks  UBE2H for the pathways selected above  
About GeneDecksing
UniProtKB/Swiss-Prot: UBE2H_HUMAN, P62256
Pathway: Protein modification; protein ubiquitination


5/13 Interacting proteins for UBE2H (ENSP000003478363) via UniProtKB, MINT, and/or STRING (see all 13 )

InteractantInteraction Details
GeneCardExternal ID(s)
UBCENSP000003448183STRING (score=.963)
TMEM189ENSP000003441663STRING (score=.918)
ANAPC11ENSP000003499573STRING (score=.909)
PARP1ENSP000003557593STRING (score=.9)
UBA1ENSP000003384133STRING (score=.898)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process TAS8132613
GO:0016567 protein ubiquitination IEA--
GO:0051246 regulation of protein metabolic process IEA--
About this table

Drugs & Compounds
for UBE2H

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2H

Transcripts
for UBE2H

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (UBE2H)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2H)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003344

              Sigma-Aldrich siRNA and siRNA Panels for UBE2H  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2H  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003344  NM_182697  

REFSEQ mRNAs for UBE2H gene (2 alternative transcripts): 

NM_003344.2   NM_182697.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003344  NM_182697  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003344
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003344
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003344 

Additional cDNA sequence: 

AB451490.1 AK316590.1 AY302138.1 BC006277.2 BT006756.1 CR600409.1 CR604761.1 Z29328.1 
Z29330.1 Z29331.1 

24 DOTS entries:

DT.87017898  DT.87017436  DT.95243234  DT.99980512  DT.91994447  DT.91647119  DT.40300964  DT.121119802 
DT.100845527  DT.92008696  DT.95243162  DT.100845532  DT.40235291  DT.91886072  DT.100729584  DT.100845530 
DT.121119701  DT.121119747  DT.121119759  DT.91761141  DT.99989542  DT.91761162  DT.92406927  DT.95243169 

24/938 AceView cDNA sequences (see all 938 ):

CA447405 AI698973 AA262789 BE220446 AA831316 AA045443 BE326288 AA251789 
NM_003344 AI298176 BT006756 AI291574 AI698024 BQ188002 AI424926 AA613942 
AA670349 AA458858 AI912906 BU184072 AI246731 AW051443 AA551118 AA731706 

highest scoring ESTs for UBE2H:

AI929144 AA043199 AA287843 AA382220 AA627409 AA719538 AA836329 AA846218 AA846629 AA992519 

Unigene Cluster for UBE2H:

Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Hs.643548  [show with all ESTs]
Unigene Representative Sequence: NM_003344


GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:
ENST00000355621  ENST00000354414  

Expression
for UBE2H

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2H expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2H

1 / 2 / 3

12 probe-sets matching UBE2H gene


Affymetrix
probe-set		 Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

369_s_at2, 3 U95-A 1 1.00 1.00 0.93 0.97 Z29331 0.80 1.00 0.91 1

91147_at2, 3 U95-E 1 0.88 1.00 0.80 1.05 AI929144 1.00 1.00 1.00 1

222420_s_at2, 3 U133-B 1 1.00 1.00 -- -- Z29331 0.80 1.00 0.91 1

222421_at2, 3 U133-B 1 1.00 1.00 -- -- BF435617 0.60 1.00 0.82 1

222419_x_at2, 3 U133-B 1 1.00 1.00 -- -- AW205983 0.80 1.00 0.91 1

217799_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003344 0.60 1.00 0.82 1

221962_s_at2, 3 U133-A 1 1.00 1.00 -- -- AI829920 0.80 1.00 0.91 1

222420_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222421_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222419_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

217799_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

221962_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AGAAGCAGAC

SOURCE GeneReport for Unigene cluster: Hs.643548

Expression variation in blood from EXPOLDB for UBE2H

Orthologs
for UBE2H

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section
Orthologs for UBE2H gene from 5/19 species (see all 19 )
Organism Gene Locus Description Human
Similarity		 NCBI accessions
dog
(Canis familiaris)		 UBE2H1   -- ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 96.72(n)
100(a)		 607816  XM_843755.1  XP_848848.1 
chimpanzee
(Pan troglodytes)		 UBE2H1   -- ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 99.8(n)
100(a)		 741944  XM_001144807.1  XP_001144807.1 
cow
(Bos taurus)		 UBE2H1   -- ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 95.08(n)
100(a)		 539313  NM_001037614.1  NP_001032703.1 
mouse
(Mus musculus)		 Ube2h1, 5 6 (6.50 cM)5
 ubiquitin-conjugating enzyme E2H1, 5 93.81(n)1
100(a)1		 222141  NM_009459.21  NP_033485.11 
 AI1818395  AI3269655  (see all 18)
chicken
(Gallus gallus)		 UBE2H1   -- ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 89.25(n)
100(a)		 416678  NM_001030647.1  NP_001025818.1 
About this table        Species with no ortholog for UBE2H

ENSEMBL Gene Tree for UBE2H

Paralogs
for UBE2H

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --

SNPs/Variants
for UBE2H

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/620 NCBI SNPs in UBE2H are shown (see all 620 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 161)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 7 posSequenceRecsAA
Chg		TypeMoreRecsAllele
freq		PopTotal
sample		More
------------
rs69638611,2
A,C,F,H129380575(+) TAGTAC/TTAATA 2 -- ng314Minor allele frequency- T:0.34EU EA WA 412
rs10458971,2
A,C,F,H129379746(-) GGGACA/CGCGGG 2 -- ut51 ese31Minor allele frequency- C:0.47WA 112
rs10466911,2
C,F,H129260975(-) CCCACG/ATGGTG 2 -- ut3113Minor allele frequency- A:0.23MN NA EU EA WA 1348
--
rs26924421,2
C,F,H129381053(-) GGCTGT/CGTAAA 2 -- ng314Minor allele frequency- C:0.01EU EA WA 416
--
rs76391,2
C,F,H129260667(-) TCCTGC/TGTATG 2 -- ut3110Minor allele frequency- T:0.17MN EU EA WA 1208
--
rs69641871,2
C129380801(+) gctggC/Tggatc 2 -- ng31 tfbs30--------
rs125398001,2
A,C,F129266374(+) GCCAAT/CAACTG 2 L/L syn1 ese32Minor allele frequency- C:0.06NA 182
rs27275001,2
A,C,F,H,O129262559(-) CTGACC/TGTAAT 2 -- int19Minor allele frequency- N:0.00EA NA EU WA 1940
rs14718761,2
A,C,F,H,O129313068(+) AAAATA/GACAAA 2 -- int113Minor allele frequency- G:0.22MN EU EA WA NA 1256
rs27086371,2
A,C,F,H,O129294178(+) ATATGC/TTTCCC 2 -- int17Minor allele frequency- T:0.40EU EA WA NA 552
About this table

HapMap Linkage Disequilibrium images for UBE2H (up to first 250kb)

Disorders & Mutations
for UBE2H

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

 OMIM: 601082

Human Genome Epidemiology Navigator: UBE2H (2 documents)

Medical News
for UBE2H

(Possibly Related Articles in Doctor's Guide)
About This Section

   --

Publications
for UBE2H

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/18 PubMed articles for UBE2H gene (see all 18 ):

Search for UBE2H

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  
  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

Genome Databases showing UBE2H

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section
Entrez Gene: 7328 HGNC: 12484 AceView: UBE2H Ensembl:ENSG00000186591 euGenes: HUgn7328
ECgene: UBE2H H-InvDB: UBE2H

Other Databases showing UBE2H

(According to HUGE)
About This Section

   --

Specialized Databases showing UBE2H

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

   --

Licensable Technologies
for UBE2H

(Available from WIS Yeda, Salk, Tufts)
About This Section

  --

Services
for UBE2H

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2H:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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 Predesigned and custom siRNAs for UBE2H Antibodies for UBE2H
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 Lentivirus-delivered shRNAs for UBE2H Browse small molecules at Sigma-Aldrich
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 Recombinant Proteins
(Ube2H)
 Antibodies (Ube2H)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for UBE2H




 Assays/Proteins for UBE2H
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for UBE2H

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