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UBE2G1 Gene

protein-coding   GIFtS: 60

GC17M004120
ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
(Previous names: ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7), ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: UBE2G)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
E217K 2, 3
EC 6.3.2.19 3
UBC7 2, 3
UBE2G 2, 3
Descriptions
Ubiquitin carrier protein G1 2, 3
Ubiquitin-protein ligase G1 2, 3
ubiquitin-conjugating enzyme E2G 1 2
ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C.
elegans) 1, 2
ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 2
ubiquitin-conjugating enzyme E2G 1 (homologous to C.
elegans UBC7) 1, 2
External Ids
HGNC: 124821
Entrez Gene: 73262
UniProtKB: P622533
Ensembl: ENSG000001323887
Search outside databases for aliases for UBE2G1 gene

Previous GC identifers: GC17M004492 GC17M004124 GC17M004380

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for UBE2G1:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting
abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes
of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and
ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating
enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein
may be involved in degradation of muscle-specific proteins. [provided by RefSeq]

UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in
degradation of muscle-specific proteins

Gene Wiki entry for UBE2G1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2G1 gene  

Entrez Gene cytogenetic band: 1q42|17p13.2   Ensembl cytogenetic band:  17p13.2   HGNC cytogenetic band: 17p13.2

UBE2G1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M004120:     (about GC identifiers)

Start:
4,119,261 bp from pter
End:
4,216,718 bp from pter
Size:
97,458 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000017.9  NT_010718.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 G1  
Size: 170 amino acids; 19509 Da
PDB structures from and Proteopedia :
2AWF (3D)    
Secondary accessions: Q99462

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003333.1  

    ENSEMBL proteins: 
    ENSP00000254724 ENSP00000380178 


    Human Recombinant Proteins 
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    Recombinant Proteins from Abcam (Ube2G1)
    Human Recombinant Proteins from Abnova (UBE2G1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for UBE2G1: 
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    Antibodies from Abcam (Ube2G1), each with their AbpromiseSM
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    Novus Biologicals Antibodies for UBE2G1

    Assays for UBE2G1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
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    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugat/RWD-like


       GeneDecks  UBE2G1 for the domains selected above  
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    Graphical View of Domain Structure for InterPro Entry P62253

    ProtoNet protein and cluster: P62253

    1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253
    Similarity: Belongs to the ubiquitin-conjugating enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2G1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2G1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003342

                  Applied Biosystems Silencer® siRNAs for UBE2G1

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2G1  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2G1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003342
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003342
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003342 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003342

    UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253
    Function: Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in
    degradation of muscle-specific proteins
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
    N-ubiquityllysine
    Enzyme Number (IUBMB): EC 6.3.2.19 

    Genatlas biochemistry entry for UBE2G1:
    ubiquitin conjugating enzyme E2G,highly homolog to C elegans UBC7,highly expressed in skeletal
    muscle

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842 ubiquitin-protein ligase activity IEA--
    GO:0016874 ligase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for UBE2G1
     Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway

       GeneDecks  UBE2G1 for the pathways selected above  
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    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2G1  (Your Favorite Gene powered by Ingenuity) 
     Protein Ubiquitination Pathway
     Hypoxia Signaling in the Cardiovascular System

       GeneDecks  UBE2G1 for the pathways selected above  
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    2 Kegg Pathways  (Kegg details for UBE2G1):
     hsa04120 Ubiquitin mediated proteolysis
     hsa05012 Parkinson's disease

       GeneDecks  UBE2G1 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253
    Pathway: Protein modification; protein ubiquitination


    1 Interacting protein for UBE2G1 (ENSP000002547243) via UniProtKB, MINT, and/or STRING

    InteractantInteraction Details
    GeneCardExternal ID(s)
    AMFRENSP000002906493STRING (score=.804)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006511 ubiquitin-dependent protein catabolic process TAS8893823
    GO:0043687 post-translational protein modification IEA--
    GO:0051246 regulation of protein metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBE2G1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2G1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2G1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003342

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2G1  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2G1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003342  

    REFSEQ mRNAs for UBE2G1 gene: 

    NM_003342.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003342  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003342
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003342
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003342 

    Additional cDNA sequence: 

    AK313059.1 BC002775.2 BC017006.1 BC026288.1 BT007416.1 CR622319.1 CR623407.1 D78514.1 

    10 DOTS entries:

    DT.444928  DT.97847027  DT.95333171  DT.100789880  DT.100789882  DT.91751363  DT.120910165  DT.445070 
    DT.75172367  DT.91742715 

    24/350 AceView cDNA sequences (see all 350 ):

    BQ924413 BM723498 BG112905 CR623407 BC026288 AW150446 BG677778 BG676464 
    AW008557 Z42296 AI364886 AW499792 BU568576 BC002775 BU161770 Z42739 
    BF982430 BE673128 AA721173 BI858731 AI928773 AW016088 AA593118 AL138101 

    highest scoring ESTs for UBE2G1:

    D78514 AA113903 AU137034 BE396516 BE898059 BF981447 BG112905 BG115232 BG116490 BG289576 

    Unigene Cluster for UBE2G1:

    Ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
    Hs.714345  [show with all ESTs]
    Unigene Representative Sequence: NM_003342


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2G1 (see all 6 )

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c
    SP1:                          -                                                               
    SP2:                          -                       -     -     -                           
    SP3:                          -                                                               
    SP4:                                                  -                                       
    SP5:                          -                       -                                       

    About this scheme

    ECgene alternative splicing isoforms for UBE2G1

    2 Ensembl transcripts including schematic representations:
    ENST00000254724  ENST00000396981  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBE2G1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBE2G1

    1 / 2 / 3

    9 probe-sets matching UBE2G1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    1423_at2, 3 U95-A 1 1.00 1.00 0.95 0.94 D78514 1.00 0.90 0.95 1
    37662_at2, 3 U95-A 1 1.00 1.00 0.95 0.86 AI701164 0.80 0.80 0.80 1

    49474_at2, 3 U95-B 1 1.00 1.00 0.86 1.21 AI191413 0.40 1.00 0.76 1

    209141_at2, 3 U133-A 1 1.00 1.00 -- -- AW299555 0.60 1.00 0.82 1

    226005_at2, 3 U133-B 1 1.00 1.00 -- -- BG170762 0.40 1.00 0.76 1

    209142_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC002775 0.80 0.88 0.84 1

    209141_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    226005_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    209142_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  UBE2G1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGATGAGTGC

    SOURCE GeneReport for Unigene cluster: Hs.714345

    UniProtKB/Swiss-Prot: UB2G1_HUMAN, P62253
    Tissue specificity: Widely expressed, mainly in skeletal muscle

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBE2G1 gene from 5/22 species (see all 22 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    UBE2G11   -- ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 99.15(n)
    98.73(a)
    750513  XM_001174528.1  XP_001174528.1 
    cow
    (Bos taurus)
    UBE2G11   -- ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 95.29(n)
    100(a)
    613676  XM_864424.2  XP_869517.1 
    rat
    (Rattus norvegicus)
    Ube2g11   -- ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. more 95.69(n)
    100(a)
    64631  NM_022690.2  NP_073181.1 
    mouse
    (Mus musculus)
    Ube2g11, 5 115
    ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. more1, 5 95.29(n)1
    100(a)1
    671281  NM_025985.41  NP_080261.21 
     AI2567955  AK0125395  (see all 22)
    chicken
    (Gallus gallus)
    RCJMB04_6c151   -- ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 87.66(n)
    100(a)
    770961  XM_001234278.1  XP_001234279.1 
    About this table        Species with no ortholog for UBE2G1

    ENSEMBL Gene Tree for UBE2G1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBE2G1 gene
    UBE2G22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/548 NCBI SNPs in UBE2G1 are shown (see all 548 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 115)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10457381,2
    A,C,F,H,O4119692(-) TTGACT/GTGTCA 1 -- ut31 ese312Minor allele frequency- G:0.32EA MN EU WA NA 2508
    rs80645961,2
    A,F,H4218541(+) gagctA/Ggagac 1 -- ng311Minor allele frequency- G:0.29EU 120
    --
    rs99894591,2
    A,C4217987(+) acctcC/Tgcctc 1 -- ng310--------
    --
    rs99894381,2
    A,C4218058(+) gcgcaC/Tgcctc 1 -- ng310--------
    rs47906021,2
    A,C4118866(+) TAACTG/TACTAA 1 -- ng510--------
    rs283625471,2
    F4121321(-) GTTCTA/GTCTAG 1 -- ut312Minor allele frequency- G:0.01MN EA 1252
    rs18661761,2
    A,C,F,H,O4152194(+) CCTTTC/TATTGG 1 -- int111Minor allele frequency- T:0.41EA NA EU WA 706
    rs7336221,2
    A,C,F,H,O4127871(+) AAGCCA/GTGAGA 1 -- int122Minor allele frequency- G:0.43EA NA EU WA 1817
    rs23259411,2
    A,C,F,H,O4153475(+) GGAACG/ATTTGA 1 -- int17Minor allele frequency- A:0.05EU EA WA NA 554
    --
    rs129500481,2
    A,C,F,H4162497(+) TGAAAT/CAggga 1 -- int16Minor allele frequency- C:0.30EU EA NA 382
    About this table

    HapMap Linkage Disequilibrium images for UBE2G1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601569

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/14 PubMed articles for UBE2G1 gene (see all 14 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7326 HGNC: 12482 AceView: UBE2G1 Ensembl:ENSG00000132388 euGenes: HUgn7326
    ECgene: UBE2G1 H-InvDB: UBE2G1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBE2G1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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